RP—HPLC法测定复方氧氟沙星栓剂中氧氟沙星和甲硝唑的含量

目的：建立反相高效液相色谱法,测定复方氧氟沙星栓剂中氧氟沙星和甲硝唑的含量。方法：采用ＯＤＳ－Ｃ１８（４．６×２５０ｍｍ）５μｍ不锈钢柱,以盐酸雷尼替丁为内标物,甲醇：水：磷酸：乙乙胺（３０：７０：０．３９：０．７０;ｐＨ＝３．１５）为流动相,检测波长２７５ｎｍ。结果：氧氟沙星在３．０￣２６．０μｇ·ｍｌ＾－１范围内线性关系良好,ｒ＝０．９９９９（ｎ＝５）,平均回收率为９８．８２％,ＲＳＤ＝０．７     

高效毛细管电泳法测定何首乌中二苯乙烯甙的含量

目的：建立测定何首乌中二苯乙烯甙含量的高效毛细管电泳法。方法：电解液为２０ｍｍｏｌ·Ｌ＾－１硼砂,检测波长２１４ｎｍ,二苯乙烯甙为对照品,测定了何首乌及其制剂中二苯乙烯甙的含量。结果：进样量在１０．０￣１００．０μｇ·ｍｌ＾－１范围内线性关系良好（ｒ＝０．０９９９９）,加样回收率为９８．７１％,ＲＳＤ为１．５１％（ｎ＝３）,测得生何首乌,制何首乌及优生宝胶囊中二苯乙烯甙的含量分别为０．１２７％、０     

高效液相色谱法测定疏络止痛胶囊中粉防己碱的含量

目的：测定疏络止痛胶囊中粉防己碱的含量。方法：ＲＰ－ ＨＰＬＣ法选用Ｃ１８ 分析柱,流动相：甲醇－ 水－ 二乙胺（８２∶１８∶０．２） ,检测波长为２８３ｎｍ ,流速：１．０ｍｌ·ｍｉｎ－１ ,柱温：４０℃。结果：该法平均回收率为９８．１２ ％ , ＲＳＤ ＝ １．０６％ （ ｎ ＝ ５） 。结论：本法快速、准确,样品处理简便易行,重现性好。     

心肌肌钙蛋白－IRIA建立及初步临床应用

从人心肌组织中提纯并鉴定了心肌肌钙蛋白－１（ＣＴｎＩ），免疫新西兰纯系兔而获特异性抗血清，其亲和常数Ｋａ值为４．１×１０１０Ｌ／ｍｏｌ．Ｉｏｄｏｇｅｎ法制１２５Ｉ－ＣＴｎＩ，比活度为３６５９ＧＢｑ／ｇ．建立了ＣＴｎＩ双抗体ＲＩＡ，标准曲线ＥＤ５０为６．４±０．３４μｇ／Ｌ（ｘ±ｓ，ｎ＝８），灵敏度达１．５μｇ／Ｌ，工作范围为２－６４μｇ／Ｌ，批内、批间ＣＶ分别为２．１％－５．７％（ｎ＝８）和５．４％－９．０％（ｎ＝４），方法回收率为９０．１％－９５．３％．与骨胳肌肌钙蛋白－Ｉ（ＳＴｎＩ）未见交叉反应，测定８７例正常人血清ＣＴｎＩ浓度为２．１７±０．６６μｇ／Ｌ（ｘ±ｓ），８０例有关疾病患者ＣＴｎＩ血清浓度测定表明该方法对诊断心肌细胞损伤具有心肌特异性．并且ＣＴｎＩ于心肌细胞损伤后在血清中出现时间早，维持时间长，是一个理想的心肌细胞损伤标志物．     

高效液相色谱法测定人血清中伊曲康唑浓度

目的：本文建立了ＨＰＬＣ法测定血清样品中伊曲康唑的定量分析方法。方法：色谱条件：以ＺＯＲＢＡＸＴＭ－ Ｃ１８（５μｍ ,４－６×１５０ｍｍ） 为色谱柱;乙晴－ 水（６７－５∶３２－５Ｖ／Ｖ） 为流动相,检测波长２６３ｎｍ ;用正庚烷：异戊醇（９８－５∶１－５Ｖ／Ｖ） 作为提取液。结果：３ 种浓度４０、８０、３００ｎｇ·ｍｌ－１ 回收率分别为１０５－０５％ 、１００－５７ ％ 、９７－９１％ （ ｎ ＝ ６） ;日内、日间ＲＳＤ 分别为３－８３％ 、４－０５％ 、３－０９ ％ 及６－００％ 、４－９０ ％ 、４－７２％ （ ｎ ＝ ６）,血清中药物的最低检测浓度为５ｎｇ·ｍｌ－ １,在５ ～６００ｎｇ·ｍｌ－１ 血药浓度范围内线性关系良好,ｒ ＝０－９９９５。结论：本方法灵敏、准确、结果令人满意,适用于临床血药浓度监测     

HPLC法测定丁香油—β—环糊精中丁香酚的含量

目的：测定丁香油－β－环糊精中丁香酚的含量。方法：固定相：Ｗａｔｅｒｓ Ｓｙｍｍｅｔｒｙ Ｃ１８柱（２５０ｍｍ×４．６ｍｍ,５μｍ）,流动相：甲醇－水（６５：３５）为,流速０．８ｍｌ／ｍｉｎ,检测波长２８０ｎｍ,选用丹皮酚作为人标物。结果：平均加样回收率为９７．５０％,ＲＳＤ＝０．４９％（ｎ＝６）;在０．０５￣１．６μｇ范围内线性关系良好（ｒ＝０．９９９２）。结论：本法快速、准确,样品处理简便易行     

高效液相色谱法测定顽痹通胶囊中士的宁的含量

目的：建立测定顽痹通胶囊中士的宁含量的高效液相色谱法。方法：采用高效液相色谱法。以ＺＯＲＢＡＸＲＸ－ ＳＩＬ（１５０ｍｍ ×４．６ｍｍ ５μｍ） 为色谱柱;流动相：正已烷－ 二氯甲烷－ 甲醇－ 浓氨水（２７０∶２７０∶２０∶１．７）;检测波长：２５４ｎｍ ;流速：１．０ｍｌ·ｍｉｎ－１ 。结果：士的宁的加样回收率为９７．８２％ ,ＲＳＤ为１．８５％（ ｎ ＝ ５）。结论：该方法准确、灵敏度高,重现性好。     

高效液相色谱法测定退烧冲剂中黄芩苷的含量

目的：测定退烧冲剂中黄芩苷的含量。方法：采用ＨＰＬＣ法,Ｃ１８ 柱,流动相醋酸缓冲液（ｐＨ４．５）－ 甲醇（１∶１）,检测波长２５４ｎｍ 。结果：黄芩苷在０．５～１０ｍｇ·Ｌ－１ 浓度范围内线性关系良好,ｒ ＝ ０．９９９８,平均回收率９８．６％ , ＲＳＤ 为１．５９％ 。结论：所建立的含量测定方法简便、准确,可作为该产品的质量控制方法。     

高效液相色谱法测定复方庆大霉素合剂中4种主要成分

目的：建立测定复方庆大霉素合剂中盐酸普鲁卡因、盐酸麻黄碱、对羟基苯甲酸乙酯和硫酸庆大霉素的高效液相色谱法。方法：前３ 种药物用反相色谱－ 波长和灵敏度切换法同时测定，硫酸庆大霉素用柱前衍生化色谱法测定。结果：质控结果显示药物浓度与峰高响应值线性关系良好，相关系数分别０－９９９５，０－９９７８，０－９９６６ 和０－９９９２；４ 种药物的加样回收率分别平均为９５－５±５－６％ ，９６－２±４－５％ ，９６－０±４－７％ ，９２－５±６－２％ ；日内ＲＳＤ 均＜ ６－０ ％ （ ｎ ＝５），连续测定５ｄ 的日间ＲＳＤ ＜ ７－２ ％（ ｎ ＝５），精密度和重现性良好。结论：该法适于作为药检室检测该合剂的方法     

高效液相色谱法测定复方美沙芬胶囊中美沙芬与布洛芬含量

目的：建立复方美沙芬胶囊中美沙芬与布洛芬的高效液相色谱测定法。方法：固定相Ｓｈｉｍｐａｃｋ ＣＬＣ－ ＯＤＳ柱,流动相乙腈－ 水（６５∶３５,含０．００７ｍｏｌ·Ｌ－１ 硝酸铵、０．００７ｍｏｌ·Ｌ－ １ ＳＤＳ,用冰醋酸调ｐＨ至３．４）,流速１．０ｍｌ·ｍｉｎ－１,在２７０ｎｍ 处测定。结果：美沙芬含量线性范围０ ．０２ ～０．１０ｍｇ·ｍｌ－１ ,回归方程：Ｙ＝２．５１×１０３ ＋１．８４×１０６Ｘ,ｒ ＝０．９９９６ ;布洛芬含量线性范围０ ．２０～１．００ｍｇ·ｍｌ－１ ,Ｙ＝ １．８６×１０３＋ ５．１６×１０５Ｘ,ｒ ＝０．９９９７ 。结论：含量测定方法简便快速,结果准确,可用于该制剂的质量控制。     

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version

The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

Do Balance Board Training Programs Reduce the Risk of Ankle Sprains in Athletes?

Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).     

High Intensity Interval Training:New Insights

KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief，intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.，≥90%of VO2 peak).     

Endovascular management of carotid-cavernous fistulas

Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas （CCF） and their complications such as pseudoaneurysms. Methods： Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% （4/22）. A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% （8/22）. Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents.     

Developmental validation of the PowerPlex(®) ESI 16 and PowerPlex(®) ESI 17 Systems: STR multiplexes for the new European standard

In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega^® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex^® ESI 16 (European Standard Investigator 16) and the PowerPlex^® ESI 17 Systems. The PowerPlex^® ESI 16 System combines the 11 loci compatible with the UK National DNA Database^®, contained within the AmpFlSTR^® SGM Plus^® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR^® SGM Plus^® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex^® ESI 17 System amplifies the same loci as the PowerPlex^® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR^® SGM Plus^® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex^® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors.     

Prevalence and severity of hip and groin pain in sub‐elite male football: a cross‐sectional cohort study of 695 players

The purpose of this study was twofold: (a) to investigate the prevalence of hip and groin pain in sub‐elite male adult football in Denmark and (b) to explore the association between prevalence and duration of hip and groin pain in the previous season with the Copenhagen Hip and Groin Outcome Score (HAGOS) in the beginning of the new season. In total 695 respondents from 40 teams (Division 1–4) were included. Players completed in the beginning of the new season (July–Sept 2011) a self‐reported paper questionnaire on hip and/or groin pain during the previous season and HAGOS. In total 49% (95% CI: 45–52%) reported hip and/or groin pain during the previous season. Of these, 31% (95% CI: 26–36%) reported pain for >6 weeks. Players with the longest duration of pain during the previous season had the lowest HAGOS scores, when assessed at the beginning of the new season, P < 0.001. This study documents that half of sub‐elite male adult football players report pain in the hip and/or groin during a football season. The football players with the longest duration of pain in previous season displayed the lowest HAGOS scores in the beginning of the new season.     

Bronchial stump fistula :treatment with covered retrievable hinged metallic stents-preliminary clinical experience

Objective To evaluate the preliminaily clinical efficacy and retrievability of a retrievable hinged covered metallic stent in the treatment of the bronchial stump fistula （BSF）. Methods Between April 2003 and March 2005, 8 patients with bronchial stump fistula after pneumonectomy or lobectomy were treated with two types （A and B） of retrievable hinged covered metallic stents. Type A stent was placed in 6 patients and type B in 2 under fluoroscopic guidance. The stent was removed with a retrieval set when BSF was healed or complications occurred. Results Stent placement in the bronchial tree was technically successful in all patients, without procedure-related complications. Immediate closure of the BSF was achieved in all patients after the procedure. Stents were removed from all patients but one. Removal of the stents was difficult in two patients due to tissue hyperplasia. Patients were followed up for 6 - 21 months. Placement of the stents remained stable in all patients except one due to severe cough. Permanent closure of BSF was achieved in 7 （87.5%） of 8 patients. Conclusion Use of a retrievable hinged covered expandable metallic stent is a simple, safe, and effective procedure for closure of the BSF. Retrieval of the stent seems to be feasible. （J Intervent Radiol, 2007, 16： 253-257）