Atorvastatin attenuates oxidative stress in Alzheimer＇s disease

Objective： To investigate serum level of SOD, MDA, ox-LDL, AchE and Ach in AD, to study atorvastatin influence on serum level of SOD, MDA, ox-LDL, Ache and Ach in AD and its neuroprotection mechanisms. Methods Subjects were divided into： normal blood lipid level group with Alzheimer＇s disease （A）, higher blood lipid level group with Alzheimer＇s disease （AH）, normal blood lipid level Alzheimer＇s disease group with atorvastatin treeatment （AT）, higher blood lipid level Alzheimer＇s disease group with atorvastatin treeatment（AHT）. Ox-LDL was measured by enzyme linked immunosorbent assay; SOD, MDA, ox-LDL, AchE, Ach and blood lipid level in AD was measured by biochemistry. Results： The serum level of MDA, Ache in AH group after atorvastatin treatment is lower ;The serum level of SOD, Ach in AH group is more increased than that of in A group; The serum level of ox-LDL in AH, A groups is lower than that of in A group; The dementia degree is lower after atorvastatin treatment. Conclusion： Atorvastatin can decrease serum level of MDA, AchE and ox-LDL, and increase that of SOD, Ach, and attenuate dementia symptom in AD, especially, with hyperlipemia. The hypothesis of atorvastatin neuroprotection is concluded that atorvastatin may restrain free radical reaction and retard oxidation in AD.     

The serum levels of MMP-9, slCAM-1, CRP and WBC increased in patients with acute coronary syndrome

Objective：To evaluate the association of the peripheral levels of the defined inflammatory markers with different types of acute coronary syndrome （ACS） and stable angina, and the role inflammation played in the pathogenesis of ACS. Methods ：For understanding the variation of serum concentrations of matrix metalloproteinase-9 （MMP-9）, soluble intercellular adhesion molecule-1 （sICAM-1） ,C-reactive protein （CRP）, and white blood cell count （WBC） and their association with ACS, 90 patients with coronary heart disease （CHD） and 30 healthy volunteers were recruited. The enrolled people were assigned into four equal groups, including acute myocardial infarction （AMI） group, unstable angina pectoris（UAP） group, stable angina pectoris（SAP） group and healthy control group. The serum levels of MMP-9 and sICAM-1 were measured with ELISA kits, CRP were measured with im munoturbidimetric assay, and WBC number were assessed all before any treatment was administrated. Results： （1）The serum levels of MMP-9 ,sICAM-1 ,CRP and WBC in the patients with ACS were significantly higher than those in the control group （P 〈 0.01 ）. （2）Compared with control group, patients with SAP only had higher serum level of sICAM-1 （P 〈 0.01 ）. While the levels of MMP-9, CRP, and WBC had no significant difference between them （P 〉 0.05 all）. （3）Significant positive correlation between the serum levels of MMP-9 and sICAM-1 and CRP and WBC all were observed in the patients with ACS （P 〈 0.05）. Conclusion： The elevation of serum concentrations of inflammatory markers including MMP-9, slCAM-1, CRP and WBC were associated with initiation and progression of ACS, and they may help predicting cardiovascular events.     

Levels of soluble adhesion molecules in patients with various clinical presentations of coronary atherosclerosis

Background Adhesion molecules play an important role in the development and progression of coronary atherosclerosis. The aim of this study was to compare concentrations of soluble forms of adhesion molecules in patients with different clinical presentations of coronary artery disease （CAD）.Methods One hundred and twenty-eight patients with CAD were divided into three groups; the first group was acute myocardial infarction group （AMI group, n=45）, the second group was unstable angina pectoris group （UAP group, n=48）,the third group was stable angina pectoris group （SAP group, n=35）. We compared them with patients with normal coronary arteries （control group, n=31）. The serum levels of vascular cell adhesion molecule （VCAM-1）, intercellular adhesion molecule-1 （ICAM-1）, E-selectin and P-selectin were measured in all subjects.Results The serum level of VCAM-1 in the AMI group was significantly higher than in the UAP, SAP and control groups （P 〈0.01）. The level in the UAP group was significantly higher than the SAP group and control group （P 〈0.01） and the level in the SAP group was significantly higher than in the control group （P 〈0.01）. The serum ICAM-1 level was significantly elevated in the AMI, UAP and SAP groups as compared to the control group （P 〈0.01）. The levels of serum E-selectin and P-selectin in the AMI and UAP groups were significantly higher than in the SAP and control groups （P〈0.01）.Conclusions Increased levels of VCAM-1 and ICAM-1, E-selectin and P-selectin, as markers of inflammation, showed the importance of inflammatory processes in the development of atherosclerosis and clinical expression of CAD. Soluble ICAM-1, VCAM-1, E-selectin and P-selectin concentrations are useful indicators of the presence of atherosclerosis and the severity of CAD clinical presentation.     

Effect of Atorvastatin on Serum MMP-2,MMP-9 and TIMP-1 in Rabbits with Chronic Heart Failure

Objective: To observe the effects of atorvastatin on serum matrix metalloproteinase-2(MMP-2), matrix metalloproteinase-9(MMP-9), and the tissue inhibitor of metalloproteinase-1(TIMP-1) in the development of chronic heart failure. To investigate the role of atorvastatin in the therapy of chronic heart failure and determine its possible mechanism of action. Methods: Thirty Japanese Big Ear rabbits were randomly selected and divided into 3 groups: sham-operated group(SO group), heart failure control group(HC group) and heart failure atorvastatin therapy group(HA group), with 6, 12 and 12 animals in the respective groups. Volume overloading was produced in the HC group and HA group animals by creating an aortic insufficiency, induced by damaging the aortic valve with a catheter introduced through the carotid artery. After 14 days, abdominal aorta constriction was performed in order to obtain a pressure overload. Six weeks later rabbits in the HA group were administered atorvastatin 3mg. Kg-1.d-1 for 4 weeks, at which time the experiment was terminated. Arterial blood was drawn and serum levels of MMP-2, MMP-9 and TIMP-1 were measured in all groups at the same time using an ELISA method. Results: Structural and functional indicators of chronic heart failure(CHF) were seen in both the HC and HA groups, but atorvastatin significantly reduced the observed effects. The serum concentrations of MMP-2, MMP-9 and TIMP-1 were at low levels in all three groups at the start of the study, with no difference between them(P < 0.05). At the end of 6th week concentrations were significantly increased in the HC and HA groups compared with the SO group(P < 0.05), but there were no differences between the HC group and HA group(P > 0.05). The increased concentrations in HC group continued to the end of the experiment, but values in the HA group were all lower than those in the HC group by the end of the experiment(P < 0.05). Conclusion: Serum concentrations of MMP-2, MMP-9 and TIMP-1 increase significantly during the course of CHF, paralleling the pathological progress of CHF. Atorvastatin benefits CHF, and the decreased serum levels of MMP-2, MMP- 9 and TIMP-1 may be one of the drug's mechanism of action.     

Characteristics of fat metabolism in skeletal muscle of rats after hypobaric hypoxic acclimation

Objective：To investigate the characteristics of fat metabolism in rat skeletal muscle after hypobaric hypoxia acclimation. Methods： Sprague-Dawley rats were divided into 3 groups randomly： control group （H0）, hypoxic 5-day group （HS）, and hypoxic 15-day group （H15）. Animals of H5 and 15 groups were exposed to hypobaric hypoxia chamber simulating 5 000 m high altitude for 5 d or 15 d respectively, 23 h per day. H0 group stayed outside of chamber The level of fatty acid oxidation and uptake, and glucose oxidation were examined, and the level of non-esterified fatty acids （NEFA）, ATP and phosphocreatine （PCr） were also assayed in rat skeletal muscles. Results： The contents of ATP and PCr in H5 group were lower than those in H0 and H15 groups （P〈0.05）, while there was no significant difference between H0 and H15. Compared with H0, the blood NEFA level in all hypoxia groups was increased significantly （P〈0.05）. The muscle NEFA level in H15 group was greatly higher than that in H0 and H5 groups. The rates of fatty acid oxidation and uptake in H15 group were significantly higher than those in H0 and H5 groups （P〈0.05）, and the rate of glucose oxidation in all hypoxia groups was significantly decreased than that in H0 group （P〈0.05）. Conclusion： It is concluded that the enhanced fat oxidation may be one of the mechanisms in the maintenance of energy homeostasis after hypobaric hypoxic acclimation.     

Modulating Effects of Chlorogenic Acid on Lipids and Glucose Metabolism and Expression of Hepatic Peroxisome Proliferator-activated Receptor-α in Golden Hamsters Fed on High Fat Diet

Objective To examine the effects of chlorogenic acid （CGA） on lipid and glucose metabolism under a high dietary fat burden and to explore the possible role of peroxisome proliferator-activated receptor-α （PPAR-α） in these effects. Methods Twenty male golden hamsters were randomly divided into CGA treatment group （n=10, given peritoneal injection of CGA solution prepared with PBS, 80 mg CGA/kg body weight daily）, and control group （n=10, given PBS i.p. at the average volume of the treatment group）. Animals in both groups were given 15% high fat diet. Eight weeks after treatment with CGA, the level of biochemical parameters in fasting serum and tissues and the expression of hepatic mRNA and protein PPAR-α were determined. Results Eight weeks after treatment with CGA, the levels of fasting serum triglyceride （TG）, free fatty acid （FFA）, total cholesterol （TC）, low density lipoprotein cholesterol （LDL-C）, high density lipoprotein cholesterol （HDL-C）, glucose （FSG）, and insulin （FSI） were significantly lower in the GGA treatment group than in the control group. CGA also led to higher activity of hepatic lipase （HL） lower contents of TG and FFA in liver, and lower activity of lipoprotein lipase （LPL） in skeletal muscle. Furthermore, CGA significantly elevated significantly elevated the expression level of mRNA and protein expression in hepatic PPAR-α. Conclusion CGA can modify lipids and glucose metabolism, which may be attributed to PPAR-α facilitated lipid clearance in liver and improved insulin sensitivity.     

Pulmonary artery perfusion with HTK solution prevents lung injury in infants after cardiopulmonary bypass

Background Pulmonary artery perfusion during cardiopulmonary bypass （CPB） is a novel adjunctive method, which can minimize the lung ischemic-reperfusion injury and inflammatory response. This study evaluated the protective effect of pulmonary perfusion with hypothermic HTK solution in corrections of congenital heart defects with pulmonary hypertension. Methods Between June 2009 and December 2009, 24 consecutive infants with congenital heart defects and pulmonary hypertension were randomly divided into perfused group （n=12） and control group （n=-12）. Oxygen index, alveolar-arterial 02 gradient, serum levels of malondialchehyche （MDA）, interleukin （IL）-6, -8, -10, soluble intercellular adhesion molecule-1 （slCAM-1）, and P-seiectin were measured before commencement and serially for 48 hours after termination of bypass. Results Oxygenation values were better preserved in the perfused group than in the control group. The serum levels of IL-6 increased immediately after CPB in both groups and returned to baseline at 48 hours after CPB, but it was restored faster and earlier in the perfused group. The serum levels of IL-8, slCAMol, and MDA remained at baseline at each point after CPB in the perfused group and elevated significantly immediately after CPB in the control group, except for slCAM-1 The serum level of IL-10 increased immediately after CPB and decreased to baseline at 48 hours after CPB in both groups, but the IL-10 level in the perfused group was significantly higher than in the control group at 12 hours after CPB. The serum P-selectin levels in the control group immediately after CPB were significantly higher than prebypass levels. Moreover, there were no significant differences in postoperative clinical characters, except for the intubated time. Conclusion In infants with congenital heart defects, pulmonary perfusion with hypothermic HTK solution during cardiopulmonary bypass could ameliorate lung function and reduce the inflammatory response.     

Serum metrix metalloproteinase-9 combined with homocysteine, IL-6, TNF-α, CRP, HbAlc and lipid profile in the incipient diabetic nephropathy with or without macrovascular diseases

Objective ： To evaluate the changes of serum matrix metalloproteinase-9 （romp-9） in patients of incipient diabetic nephropathy with or without macrovascular disease and to analyze the factors associated with homocysteine（hcy）, interleukin-6（IL-6）, tumor necrosis factor-alpha （TNF-α）, highly sensitive C-reactive protein （hsCRP）, HbAlc and lipid profile in those patients in order to know whether this marker or other factors are more important to induce diabetic macrovascular disease. Methods： Type 2 diabetes mellitus（T2DM） subjects with incipient diabetic nephropathy with or without macrovascular disease were selected for participation and divided into 2 groups. The patients in group 1 （n= 38） used insulin, and patients in group 2 （n=34） were treated with an oral antidiabetic drug. Then serum mmp-9, hey, IL-6 and TNF-α in these patients were measured, and compared to the healthy subjects as control （n= 16）. The results were analyzed by SPSS13. Results： Serum romp-9 and hcy of the patients having incipient diabetic nephropathy with macrovascular disease were higher than that of patients without macrovascular disease （P〈0.01）. For insulin-injected patients, whether they accompanied with macrovascular diseases or not, the serum levels of romp-9, hcy, IL-6 and TNF-α were all lower, but no significant statistics compared with non-insulin used patients or the healthy subjects. The serum level of romp-9 was more correlated with the serum hcy in antidiabetic drug used patients. （P〈0. 000） Conclusion： The serum level of romp-9 plays an important role of pathogenesis in the macrovascular disease in the incipient diabetic patients, and the serum level of hcy also can reflect the severely degree of macrovascular disease in these patients, insulin can reduce these markers.     

Preventive Administration of Juanbi Capsules (蠲痹胶囊) for Knee Osteoarthritis: Effects on Serum MMP-2 and MMP-9 levels and Cartilage Repair

Objective: To explore the mechanism of action of Juanbi Capsules (蠲痹胶囊), a Chinese medicine for invigorating the kidney and replenishing qi, in preventing osteoarthritis of the knee in rabbits. Methods: Seventy-two 4-month-old, Japanese long-eared white rabbits were randomly divided into 6 groups: control (group A), model (group B), Chinese drug; high-dose (group C), Chinese drug; mid-dose (group D), Chinese drug; low-dose (group E), and drug control (group F). With the exception of the rabbits in group A, each rabbit was subjected to plaster cast fixation for 6 weeks to induce osteoarthritis. In addition, rabbits were administrated with an intragastric injection of the Chinese drug (groups C, D and E) or an aminoglucose hydrochloride capsule (group F) for 4 weeks. Blood was drawn from the central ear artery for serum MMP-2 and MMP-9 concentrations, and the knee joint cartilage was harvested for gross observation and light microscopy. Results: There were significant differences in serum MMP-2 and MMP-9 concentrations between group B and groups C, D and E (P<0.05), with no significant differences between groups D and F. Histological results showed various changes in tissue staining with treatment, with osteophyte and bone cyst formation, and superficial erosion in the articular surface of the cartilage; in some cases, the defect reached the mid-layer of the cartilage, and these changes were lower than those in the model group. Conclusion: Juanbi Capsules assist in preventing osteoarthritis in the rabbit, possibly by decreasing serum MMP-2 and MMP-9 levels.     

醒脑开窍针刺法对血管性痴呆患者IGF-1、MMP-2、MMP-9表达的影响

目的:探讨醒脑开窍针刺法对血管性痴呆患者胰岛素样生长因子-1(insulin-like growth factors,IGF-1)以及基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)、MMP-9表达的影响。方法:收集140例血管性痴呆患者,随机分为观察组和对照组,每组各70例。观察组患者采用基础治疗联合醒脑开窍针法,对照组患者采用基础治疗,并针刺观察组所取穴位的旁开0.5 cm非经非穴点。观察并比较两组患者治疗前后简易智能状态量表(mini-mantal state examination,MMSE)评分、IGF-1、MMP-2、MMP-9水平。结果:与治疗前相比,治疗后两组患者的MMSE评分、IGF-1水平均升高,MMP-2、MMP-9水平均下降,差异具有统计学意义(P0.05);与对照组相比,观察组患者的MMSE评分、IGF-1水平较高,MMP-2、MMP-9水平较低,差异具有统计学意义(P0.05)。结论:醒脑开窍针刺法能够明显改善血管性痴呆患者血清IGF-1、MMP-2、MMP-9水平,并可显著改善患者的认知功能,其作用机制可能与下调血清MMP-2、MMP-9水平,提高IGF-1的水平有关,具体作用机制有待于进一步论证研究。     

急性脑梗死血清MMPs水平及阿托伐他汀对其的影响

目的检测急性脑梗死（acute ceretral infarct ACI）患者治疗前后和对照组的血清基质金属蛋自酶-2（MMP-2）、基质金属蛋自酶-9（MMP-9）和组织型金属蛋自酶抑制剂-1（TIMP-1）的水平,评估基质金属蛋自酶在ACI的作用机制,探讨阿托伐他汀对ACI的治疗保护机制。方法实验分为ACI血脂正常组（A）、ACI高血脂组（AH组）、阿托伐他汀治疗急性ACI血脂正常组（AT组）、阿托伐他汀治疗ACI高血脂组（AHT组）。双抗体夹心ELISA法测定MMP-2、MMP-9和TIMP—1．生物化学法测定血脂。结果A、AH组经过阿托伐他汀治疗后MMP-2、MMP-9浓度明显降低．TIMP-1浓度明显增高（p〈0.05）。结论阿托伐他汀能够降低急性脑梗死患者血清MMPs水平和增高血清TIMP-1的水平．阿托伐他汀可以调节细胞外基质动态平衡防治ACI。     

中西医结合对宫腔粘连患者血清MMP-9和瘦素的影响

目的:观察中西医结合对宫腔粘连患者血清MMP-9和瘦素的影响。方法:选取宫腔镜下宫腔粘连分解术后患者40例,随机分为西药组、中西医组,每组20例,同时选取健康妇女20例为对照组。西药组采用芬吗通治疗,中西医组在西药治疗的基础上加用中药汤剂口服治疗。两组患者均治疗3个月经周期。观察治疗后临床疗效,治疗前后血清MMP-9、瘦素的变化。结果:宫腔粘连患者血清MMP-9和瘦素水平均明显低于正常对照组,经治疗后,西药组、中西医组都能增加血清MMP-9及瘦素水平。与西药组比较,中西医组能明显增加血清瘦素水平,中西医组优于西药组,差异有统计学意义(P0.01)。中西医组血清MMP-9水平高于西药组,但差异无统计学意义(P0.05)。结论:宫腔粘连患者存在血清MMP-9和瘦素水平的异常,而中药发挥疗效的作用机理之一是通过升高血清瘦素、MMP-9的水平来实现的。     

T2DM合并CHD患者血清MMP-9与炎性因子的相关性研究

目的通过观察2型糖尿病(T2DM)及合并冠心病(CHD)患者血清基质金属蛋白酶-9(MMP-9)、高敏C反应蛋白(hs-CRP)、白介素-6、18(IL-6、IL-18)水平变化,探讨血清MMP-9与炎性因子的相关性及其临床价值。方法选择临床确诊的182例T2DM患者,据诊断标准分为T2DM组和T2DM合并CHD(T2DM+CHD)组,酶联免疫吸附(ELISA)法检测血清MMP-9、IL-6及IL-18水平,超敏乳胶增强免疫比浊法检测血清hs-CRP水平,检测结果进行统计学分析。结果①T2DM组的血清MMP-9、hs-CRP、IL-6及IL-18水平分别显著高于对照组(P<0.01);T2DM+CHD组的血清MMP-9、hs-CRP、IL-6及IL-18水平分别显著高于T2DM组(P<0.01)。②T2DM+CHD组血清MMP-9水平分别与hs-CRP、IL-6及IL-18呈显著性正相关(r=0.672,0.591,0.603,P<0.01),相关性良好。结论 T2DM患者体内存在MMP-9与炎性因子水平的异常表达,合并CHD患者尤为显著,且血清MMP-9与炎性因子相关联。MMP-9与炎性因子参与了T2DM并发CHD的发生发展,此为早期干预提供了理论依据。     

急性缺血性脑卒中患者血清MMP-9与卒中相关性肺炎的相关性

目的 探讨急性缺血性脑卒中（acute ischemic stroke,AIS）患者入院血清基质金属蛋白酶9（matrix metalloproteinase-9,MMP-9）水平与卒中相关性肺炎（stroke associated pneumonia,SAP）的相关性。方法 回顾性分析2020年8月至2022年8月在金华市中心医院首次确诊AIS的96例患者作为研究对象,根据SAP诊断标准分为SAP组（n=40）和非SAP组（n=56）。比较两组入院美国国立卫生研究院卒中量表（National Institute of Health stroke scale,NIHSS）评分和SAP风险评分（SAP risk assessment,A2DS2）、TOAST分型、血脂和空腹血糖（fasting blood glucose,FBG）、血清白细胞计数、尿酸和同型半胱氨酸（homocysteine,Hcy）、超敏C反应蛋白（high sensitivity C-reactive protein,hs-CRP）、降钙素原（procalcitonin,PCT）和MMP-9水平。结果 SAP组入院NIHSS和A2DS2评分高于非SAP组,吞咽障碍增多,血清尿酸、hs-CRP、PCT和MMP-9水平升高,差异均有统计学意义（P<0.05）。多因素Logistic回归分析显示,入院NIHSS评分、血清hs-CRP、PCT和MMP-9水平升高是SAP的独立危险因素（P<0.05）。Spearman检验显示,血清MMP-9与NIHSS评分、hs-CRP和PCT呈正相关（P<0.05）。受试者操作特征曲线显示,入院NIHSS评分、血清hs-CRP、PCT和MMP-9水平预测SAP的曲线下面积分别为0.722[95%（confidence interval,CI）：0.659~0.804）]、0.798（95%CI：0.742~0.856）、0.831（95%CI：0.756~0.902）和0.878（95%CI：0.833~0.945）,预测价值较高。结论 MMP-9不仅具有组织蛋白溶解活性,同时具有炎症介质特性。AIS患者入院血清MMP-9水平升高可能与SAP发生密切相关,可作为SAP早期诊断的重要新型生化标志物。     

血小板输注对晚期肺癌患者血清中MMP-9、MMP-2及VEGF水平的影响

目的 探讨血小板输注对晚期肺癌患者血清中金属基质蛋白酶9 (metallomatrix protease9,MMP-9)、金属基质蛋白酶2(metallomatrix protease2,MMP-2)及血管内皮生长因子（vascular endothelial growth factor,VEGF）水平的影响,观察输注的血小板是否能促进晚期肺癌的转移。 方法 选取输注血小板的89例晚期肺癌患者,按照血小板输注是否有效［血小板增加指数(corrected count increment,CCI)＞7.5为输注有效］分成血小板输注有效组和无效组,比较2组血小板输注前及血小板输注后（12~24 h）血清中MMP-9、MMP-2及VEGF水平的变化。 结果 研究发现血小板输注有效率为80.90%（72/89）。在血小板输注后,有效组血清中MMP-9、MMP-2及VEGF水平明显高于无效组,有效组血清中MMP-9、MMP-2及VEGF水平高于血小板输注前,差异均有统计学意义（P＜0.05）。 结论 输注有效的血小板能使晚期肺癌患者血清MMP-9、MMP-2及VEGF的水平升高,可能对肺癌的侵袭和转移有促进作用,提示在给肺癌患者输注血小板时应该更谨慎。     

COX-2和MMP-9在乳腺癌患者血清中检测的意义

目的探讨乳腺癌患者血清COX-2与MMP-9的表达及其意义。方法采用ELISA法检测乳腺癌组30例,乳腺良性病变组23例,健康对照组20例血清中COX-2与MMP-9的水平。结果 （1）乳腺癌组血清COX-2水平高于乳腺良性病变组及健康对照组,乳腺癌组血清MMP-9也高于乳腺良性病变组及健康对照组,两者在3组间比较差异均有统计学意义（P〈0.05）;（2）两者联合检测对乳腺癌诊断灵敏度为91.3%,特异度为75.2%。结论血清COX-2与MMP-9的水平在一定程度上反映了乳腺癌的生物学特性;两者联合检测可提高乳腺癌诊断的灵敏度,对乳腺癌的辅助诊断可能有一定意义。     

普罗布考对颈动脉硬化症的抗氧化和抗炎症作用

目的:观察口服普罗布考对颈动脉硬化症患者氧化低密度脂蛋白(ox-LDL)及血清炎性指标水平的变化,探讨普罗布考对颈动脉硬化症的抗氧化、抗炎症作用。方法:将72例颈动脉硬化症患者随机分为两组:普罗布考组38例,在常规药物治疗的基础上口服普罗布考片0.5g,2次/d,持续12周;对照组34例,常规药物治疗,不服用任何调脂药物及抗氧化剂。分别于治疗前后检测血清ox-LDL、高敏C反应蛋白(hs-CRP)、白细胞介素-lβ(IL-lβ)、基质金属蛋白酶-9(MMP-9)水平及血脂系列等。结果:普罗布考组治疗后总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、ox-LDL、hs-CRP、IL-lβ、MMP-9显著下降(P<0.05);对照组治疗后上述指标均无显著变化。治疗后两组间TC、LDL-C、ox-LDL、hs-CRP、IL-lβ、MMP-9差异有统计学意义(P<0.05)。结论:普罗布考可降低颈动脉硬化症患者的TC、LDL-C、ox-LDL、hs-CRP、IL-lβ和MMP-9水平,并具有一定程度的抗氧化和抑制动脉粥样硬化慢性炎症过程的作用。     

MMP-3、MMP-9及TIMP-1在急性心肌梗塞中的表达及其意义

目的: 观察急性心肌梗塞（AMI）患者血清中基质金属蛋白酶-3（MMP-3）、基质金属蛋白酶-9（MMP-9）以及基质金属蛋白酶抑制剂-1（TIMP-1）水平的变化,探讨冠状动脉粥样硬化斑块破裂的机制。方法:选择67例AMI患者,发病3　h～5　d,选择同期住院非冠心病患者40例作为对照组,应用ELISA法测定血清MMP-3、MMP-9、TIMP-1含量以及血清肌钙蛋白Ⅰ（cTnⅠ）、肌酸激酶-MB（CK-MB）水平。结果：与对照组比较,AMI组血清中MMP-3、MMP-9和TIMP-1含量明显增高（P<0.05或P<0.01）;AMI组MMP-3/TIMP-1比值和MMP-9/TIMP-1比值均高于对照组（P<0.05）。MMP-3、MMP-9、TIMP-1的含量与cTnI、CK-MB水平无相关性。结论：MMP-3、MMP-9过度表达以及MMP-3/TIMP-1、MMP-9/TIMP-1比例失衡,参与冠状动脉粥样斑块的破裂和心肌梗塞的发生。