PERIPHERAL NERVE REPAIR IN RATS USING VASCULARIZED FROZEN IN-SITU MUSCLE AUTOGRAFT

ＰＥＲＩＰＨＥＲＡＬＮＥＲＶＥＲＥＰＡＩＲＩＮＲＡＴＳＵＳＩＮＧＶＡＳＣＵＬＡＲＩＺＥＤＦＲＯＺＥＮＩＮ┐ＳＩＴＵＭＵＳＣＬＥＡＵＴＯＧＲＡＦＴＷＡＮＧＹａｎ（王岩）１，ＺＨＵＳｈｅｎｇ－ｘｉｕ（朱盛修）１，ＨＵＮＧＬＫ２，ＬＥＵＮＧＰＣ２１．Ｄｅｐ...     

PCR-SSCP在恶性血液病N-ras癌基因点突变检测中的应用

目的：检测血液系统肿瘤中Ｎ－ｒａｓ癌基因点突变的活性。方法：采用ＰＣＲＳＳＣＰ技术分析了２８例恶性血液病Ｎｒａｓ基因的突变活性，包括：ＡＮＬＬ６例，ＡＬＬ１２例，ＨＤ２例，ＮＨＬ３例，ＭＤＳ５例。结果：２８例中ＰＣＲＳＳＣＰ检测与正常对照，发生阳性者４例（１４．６％），其中ＡＬＬ１例（８．３％）、ＡＮＬＬ１例（１６．６％）、ＭＤＳ２例（４０％）。４例中有２例临床缓解后持续阳性，１例为ＡＬＬ患者，于骨髓移植后１０４ｄ复发死亡。１例ＭＤＳ患者转化为急性白血病，另２例临床缓解后３个月检测转阴，其中１例为ＡＮＬＬ，１例为ＭＤＳ。结论：ＰＣＲＳＳＣＰ方法可作为一种癌基因点突变检测手段常规应用于临床，但值得注意的是，ＰＣＲＳＳＣＰ方法只能提供分析区域是否存在突变点，而不能提供突变性质的信息     

PCNA基因反义RNA表达质粒的构建及对人胃癌裸鼠移植瘤的抑制效应

为观察ＰＣＮＡ基因反义ＲＮＡ表达质粒对人胃癌裸鼠移植瘤的抑制效应,用ＤＮＡ重组法将人ＰＣ－ＮＡ基因反向克隆到真核细胞表达质粒ｐＤＯＲ－ｎｅｏ中,构建成ＰＣＮＡ基因真核表达质粒ｐＤＲ－ＰＣＮＡ,用ＬｉｐｏｆｅｃｔＡＭＩＮＴＭ介导转染人胃癌细胞系ＳＧＣ－７９０１,并接种于裸鼠背部皮下。经Ｇ４１８筛选获得的转染细胞系ＳＧＣ／ＰＣＮＡ与亲本细胞相比,其生长速度减慢,ＲＮＡ、蛋白质生物合成受到抑制,Ｓ期、Ｇ２／Ｍ期ＤＮＡ含量降低,移植瘤生长速度减慢,瘤体平均直径（０．５４±０．１３）ｃｍ,明显小于对照组（１．５１±０．１１）ｃｍ。结果表明,ＰＣＮＡ基因反义ＲＮＡ表达质粒对胃癌裸鼠移植瘤生长具有明显的抑制作用。     

A Comparison in Healing of Skull Defect Repaired with Four Different Kinds of Graft Materials in Rabbits

ＡＣＯＭＰＡＲＩＳＯＮＩＮＨＥＡＬＩＮＧＯＦＳＫＵＬＬＤＥＦＥＣＴＲＥＰＡＩＲＥＤＷＩＴＨＦＯＵＲＤＩＦＦＥＲＥＮＴＫＩＮＤＳＯＦＧＲＡＦＴＭＡＴＥＲＩＡＬＳＩＮＲＡＢ┐ＢＩＴＳＤＩＮＧＺｈｅｎ－ｑｉ（丁真奇）１，ＴＡＮＦｕ－ｓｈｅｎｇ（谭富生）２，...     

受电离辐射的细胞中p53介导增殖细胞核抗原表达的调控

增殖细胞核抗原（ＰＣＮＡ）是一种高度保守的细胞蛋白,在ＤＮＡ复制和修复中起作用。本实验用γ射线照射大鼠胚胎成纤维细胞（ＣＲＥＦ）诱导ｐ５３,研究照射后ＰＣＮＡ表达的机制。方法：实验用ｐＢＡＣＡＴ（其ＰＣＮＡ－氯霉素转移酶报告基因区域内含有人ＰＣＮＡ启动子序列）和ｐＣＭＶ－ＤＭｐ５３（表达突变型ｐ５３蛋白）两种质粒,照前２４小时用磷酸钙介导法将报告质粒转染细胞,６小时后实行甘油休克;转染２４小时后用１３７　Ｃｓγ射线照射ＣＲＥＦ１２Ｇｙ,剂量率为１．２５Ｇｙ·ｍｉｎ－１。照后在４８小时内进行细胞瞬…     

THE EFFECT OF STANDARD TOTAL PARENTERAL NUTRITION ON THE GUT BARRIER FUNCTION AND ENTEROGENIC INFECTION

ＴＨＥＥＦＦＥＣＴＯＦＳＴＡＮＤＡＲＤＴＯＴＡＬＰＡＲＥＮＴＥＲＡＬＮＵＴＲＩＴＩＯＮＯＮＴＨＥＧＵＴＢＡＲＲＩＥＲＦＵＮＣＴＩＯＮＡＮＤＥＮＴＥＲＯＧＥＮＩＣＩＮＦＥＣＴＩＯＮＡＦＴＥＲＨＥＭＯＲＲＨＡＧＩＣＳＨＯＣＫＺＨＡＯＪｕｎ（赵军），ＬＩＺ...     

GANGLIOSIDE GM_1 POTENTIATES NERVE GROWTH FACTOR EFFECTS ON REGENERATION OF CRUSHED SCIATIC NERVE IN RATS

ＧＡＮＧＬＩＯＳＩＤＥＧＭ１ＰＯＴＥＮＴＩＡＴＥＳＮＥＲＶＥＧＲＯＷＴＨＦＡＣＴＯＲＥＦ┐ＦＥＣＴＳＯＮＲＥＧＥＮＥＲＡＴＩＯＮＯＦＣＲＵＳＨＥＤＳＣＩＡＴＩＣＮＥＲＶＥＩＮＲＡＴＳＷＡＮＧＭｉｎ－ｓｈｅｎｇ（王民生），ＣＨＥＮＺｈｏｎｇ－ｗｅｉ（陈...     

离子型和非离子型造影剂在头颈部血管DSA中的应用研究

目的：探讨各种脑血管造影方法中离子型和非离子型造影剂（ＩＣＭ和ＮＩＣＭ）的合理应用及临床、技术等因素与造影剂不良反应（ＡＤＲｓ）发生率的关系。材料与方法：２６１例分别行ＩＶＤＳＡ（外围法，中心法和选择法）和ＩＡＤＳＡ（非选择法和选择法）共４５９例次造影，注射造影剂９７６次，１２例分别同时行ＩＶＤＳＡ外围法和中心法或ＩＡＤＳＡ非选择法和选择法，应用ＩＣＭ１６５例（６３．２１％），ＮＩＣＭ９６例（３６．７９％）。ＩＶＤＳＡ３７例次全部用ＩＣＭ，ＩＡＤＳＡ４２２例次用ＩＣＭ１９９例次，ＮＩＣＭ２２３例次。结果：本组ＡＤＲｓ发生率１１．１１％（２９／２６１），ＩＣＭ为１５．１５％（２５／１６５），ＮＩＣＭ为４．１６％（４／９６），两者差别有显著性（Ｐ＜０．０１），中重度反应１２例均见于经动脉注射（７例为选择性动脉注射）且经造影证实有脑内病变。结论：ＩＡＤＳＡ选择法，尤其椎动脉造影且术前已明确某些脑内病变，选用ＮＩＣＭ可明显降低ＡＤＲｓ发生率，合理选用ＩＣＭ可降低医疗费用。     

INTRADURAL HERNIATION OF LUMBAR INTERVERTEBRAL DISC：ARE－PORTOFSEVENCASESANDDISCUSSIONOFTHEPATHOLOGICALMEC

ＩＮＴＲＡＤＵＲＡＬＨＥＲＮＩＡＴＩＯＮＯＦＬＵＭＢＡＲＩＮＴＥＲＶＥＲＴＥＢＲＡＬＤＩＳＣ：ＡＲＥ－ＰＯＲＴＯＦＳＥＶＥＮＣＡＳＥＳＡＮＤＤＩＳＣＵＳＳＩＯＮＯＦＴＨＥＰＡＴＨＯＬＯＧＩＣＡＬＭＥＣＨ－ＡＮＩＳＭＬｉａｎＰｉｎｇ连平ＳｕｎＲｏｎｇ－...     

THE SIGNIFICANCE OF ADRENOCEPTORS IN THE ANTI－SHOCK EFFECTS OF TRH：PARTⅡ．STUDIES ON THE ROLE OF TRH－

ＴＨＥＳＩＧＮＩＦＩＣＡＮＣＥＯＦＡＤＲＥＮＯＣＥＰＴＯＲＳＩＮＴＨＥＡＮＴＩ－ＳＨＯＣＫＥＦＦＥＣＴＳＯＦＴＲＨ：ＰＡＲＴⅡ．ＳＴＵＤＩＥＳＯＮＴＨＥＲＯＬＥＯＦＴＲＨ－ＳＥＮＳＩ－ＴＩＺＡＴＩＯＮＯＦＡＤＲＥＮＯＣＥＰＴＯＲＳＩＮＴＨＥＡＮＴＩ－...     

Tri-allelic patterns of STRs and partially homologous non-sister chromatid crossover observed in a parentage test

A maternity testing case is reported, in which the child showed tri-allelic patterns in two short tandem repeat (STR) loci. The genotypes of Penta D of the mother and the child were 9,13 and 9,10,13, respectively. Those of D21S11 were 32.2,35 and 29,35, respectively, but intensity ratio of alleles 29 and 35 of the child was 1:2. These results suggested the copy number variations (CNVs) or trisomy of chromosome 21. By further examination using STR-based chromosome aneuploidy detection kit, three alleles were detected in D21S1411, LFG21 and Penta D, and 2 alleles with intensity ratio of 1:2 were observed in D21S2502, D21S1435, D21S11 and D21S1246. Karyotype and whole-genome SNP array analyses showed that the child had a free trisomy 21. In addition, partially homologous non-sister chromatid crossover occurred at the region 19181770-39499178 on the long arm of chromosome 21.     

用DSCR Cosmid DNA探针快速基因诊断先天愚型

目的　采用DSCRCosmidDNA探针作为检测先天愚型的特异性探针。方法　对 46例先天愚型患者外周血淋巴细胞分别在经过细胞培养和不经细胞培养两种处理条件下同时进行FISH检测 ,结果　在中期染色体和间期细胞核中观察到 3个杂交信号 ,杂交率均为 81%～ 95 %。结论　表明DSCRCosmidDNA探针可直接在未经培养的淋巴细胞间期核中进行FISH检测。 48h内能对先天愚型患者做出快速、准确的诊断。     

A dutch population study of the STR loci D21S11 and HUMFIBRA

To introduce a duplex PCR system consisting of the STR loci D21S11 and HUMFIBRA in forensic identity testing we analysed a Dutch Caucasian database of 205 individuals. The combined power of discrimination of the two loci is 0.9978 and there was no evidence for linkage equilibrium between the two loci (p=0.91). However, we noticed departure from Hardy-Weinberg equilibrium for the D21S11-locus in our database (p=0.03) but the differences between observed and expected D21S11 allele pair frequencies were of negligible practical significance in forensic calculations.     

Population genetics of ten STR loci (AmpFℓSTR SGM plus) in Austria

A population study on the ten short tandem repeat (STR) loci D3S1358, VWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01 and FGA was performed on 204 unrelated Austrian Caucasians. The DNA was amplified by multiplex PCR using the AmpFℓSTR SGM plus kit. All loci met Hardy-Weinberg expectations. The combined power of exclusion for the ten STR loci was 0.999976. The results show that these loci are very useful for forensic purposes. Received: 28 February 2000 / Accepted: 22 May 2000     

青海蒙族群体8个STR位点遗传多态性研究

目的：研究青海蒙族人群第21号染色体D21S1432、D21S1435、D21S1270、D21S1440、D21S1446、GATA24H09、ATA42C09、GATA129D11等8个STR位点的遗传多态性。方法：运用PCR扩增、6%变性聚丙烯酰胺凝胶电泳结合银染技术对30位无关个体蒙族人群进行多态性研究。结果：8个位点分别检测出6、5、7、5、6、5、5、5个等位基因片段,共140个基因型,频率分布在0.025 09~0.046 43之间,多态性分布符合Hardy-weinberg平衡定律。8个STR位点多态信息量（PolymorpHism information content,PIC）分别为0.699 4、0.703 9、0.759 2、0.638 0、0.672 0、0.685 0、0.674 0、0.691 9,累积多态信息量为0.688 9。期望杂合度（heterozygosity,HET）分别为0.740 2、0.748 3、0.790 4、0.682 2、0.741 5、0.7271、0.742 5、0.725 5,累积杂合度为0.758 9。累积个体识别率（discrimination power,DP）为0.989 6,累积排除率（probabilities of paternity exclusion,PE）为0.999 6。结论：青海蒙族8个位点STR基因座的多态性数据显示其基因分布特征具有特异性。     

超声在孕11-13~（＋6）周筛查21-三体综合征中的作用

目的：探讨孕早期超声测量胎儿鼻骨和颈项透明层厚度（NT）在筛查21-三体综合征中的作用。方法：对在苏州市母子医疗保健中心建卡的孕11-13＋6w的孕妇,随机检查3000例,重点对胎儿的颈项透明层厚度和鼻骨进行评估,并与细胞遗传学实验室确诊结果相对照。结果：除658例因体位等原因未检测出鼻骨或NT外,共成功检查出NT及鼻骨2342例。孕妇的平均年龄为26.4岁（21~42岁）,大于等于35岁占总数的2%。110例NT＆gt;3.0,2例鼻骨缺失,2例被确诊为21-三体,2例为18-三体,1例为13-三体,另4例胎儿为先天性心脏畸形。在上述2342例中,只有1例正常胎儿存在鼻骨缺失,而其NT值仍然＆gt;3.0。所以单独以鼻骨缺失为标准,21-三体的检出率为50%;单独以NT增厚为标准,21-三体的检出率为1.8%,两者相比有显著差异（P＆lt;0.01）。但通过NT来预测胎儿其他异常方面,其敏感性为64.3%,特异性为95.7%,阳性预测值8.2%,阴性预测值为99.8%,准确性为95.5%,较之鼻骨缺失,有更大的临床意义。结论：鼻骨缺失对诊断21-三体的敏感性要高于NT检查;但是,用NT增厚来预测胎儿其他方面畸形,仍有重要的临床意义。我们认为：鼻骨检测和Nrr测量相结合,对胎儿21-三体及其他畸形的筛查更有意义。     

Population data on 15 STR loci using AmpF/STR Identifiler kit in a Malay population living in and around Kuala Lumpur, Malaysia

Allele frequencies for 15 short tandem repeat (STR) loci D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA (AmpF/STR Identifiler PCR Amplification kit, PE Applied Biosystems) were obtained from a sample of 110 unrelated individuals from the Malay population living in and around Kuala Lumpur, Malaysia, and the characteristics of the population was compared with other East Asian populations.     

Unusual variant alleles in commonly used short tandem repeat loci

Unusually large variant alleles were observed in the short tandem repeat (STR) systems D3S1358 and D21S11, both of which are included in the international standard set of loci (ISSOL) and routinely typed in National DNA intelligence databases worldwide. The observed alleles fell within the size range of the adjacent STR marker, which could easily cause problems with respect to correct allele assignments for both loci concerned. We compared the amplification and potential interpretation with three different commercially available kits, which are frequently used in forensic work. PCR products were cloned and sequenced in order to determine the structure of these unusual allele variants and confirm their size and designation (D3S1358 allele 26, D21S11 allele 46). In the locus D21S11 we observed an as yet undescribed partial duplication of the constant region.     

Evaluation and application of the AmpFlSTR Profiler Plus PCR amplification kit in a Bavarian population sample

Allele frequencies for the nine tetrameric STR loci D3S1358, VWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317 and D7S820 were determined in a population sample of 155 unrelated Bavarians using the AmpFlSTR Profiler Plus PCR amplification kit. No deviations from the Hardy-Weinberg equilibrium were observed. The influence of the PCR cycle number as well as the template DNA concentration on the performance of the kit was studied. DNA concentrations lower than 75 pg DNA per 25 μl reaction volume resulted in allelic drop-out. Received: 19 April 1999 / Accepted: 27 September 1999     

Gabon black population data on the ten short tandem repeat loci D3S1358, VWA,D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01 and FGA

Allele frequencies for ten short tandem repeat (STR) loci D3S1358, VWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01 and FGA were determined in a Black African sample population from Gabon. All loci were highly polymorphic and except for TH01, D21S11 and D16S539, all met Hardy-Weinberg expectations. There was little evidence of association of alleles between the loci in this database. The combined power of exclusion for the ten STR loci was 0.999981. While significant differences between the Gabon population and the Austrian Caucasian population were found at all loci, significant differences were found between the Gabon population and Zimbabweans only for D3S1358 and between the Gabon population and African Americans only for TH01 and D8S1179. Received: 14 March 2001 / Accepted: 15 May 2001