共查询到20条相似文献,搜索用时 125 毫秒
1.
Hyunki Kim Pablo J. Arnoletti John Christein Martin J. Heslin James A. Posey III Amol Pednekar T. Mark Beasley Desiree E. Morgan 《Abdominal imaging》2014,39(4):744-752
Purpose
To confirm the feasibility of breath-hold DCE-MRI and DWI at 3T to obtain the intra-abdominal quantitative physiologic parameters, K trans, k ep, and ADC, in patients with untreated pancreatic ductal adenocarcinomas.Methods
Diffusion-weighted single-shot echo-planar imaging (DW-SS-EPI) and dynamic contrast-enhanced (DCE) MRI were used for 16 patients with newly diagnosed biopsy-proven pancreatic ductal adenocarcinomas. K trans, k ep, and apparent diffusion coefficient (ADC) values of pancreatic tumors, non-tumor adjacent pancreatic parenchyma (NAP), liver metastases, and normal liver tissues were quantitated and statistically compared.Results
Fourteen patients were able to adequately hold their breath for DCE-MRI, and 15 patients for DW-SS-EPI. Four patients had liver metastases within the 6 cm of Z axis coverage centered on the pancreatic primary tumors. K trans values (10?3 min?1) of primary pancreatic tumors, NAP, liver metastases, and normal liver tissues were 7.3 ± 4.2 (mean ± SD), 25.8 ± 14.9, 8.1 ± 5.9, and 45.1 ± 15.6, respectively, k ep values (10?2 min?1) were 3.0 ± 0.9, 7.4 ± 3.1, 5.2 ± 2.0, and 12.1 ± 2.8, respectively, and ADC values (10?3 mm2/s) were 1.3 ± 0.2, 1.6 ± 0.3, 1.1 ± 0.1, and 1.3 ± 0.1, respectively. K trans, k ep, and ADC values of primary pancreatic tumors were significantly lower than those of NAP (p < 0.05), while K trans and k ep values of liver metastases were significantly lower than those of normal liver tissues (p < 0.05).Conclusions
3T breath-hold quantitative physiologic MRI is a feasible technique that can be applied to a majority of patients with pancreatic adenocarcinomas. 相似文献2.
Jennifer G. Whisenant Todd E. Peterson Jacob U. Fluckiger Mohammed Noor Tantawy Gregory D. Ayers Thomas E. Yankeelov 《Molecular imaging and biology》2013,15(1):87-96
Purpose
The objective of this study is to determine the reproducibility of static 2-deoxy-2-[18F]fluoro-D-glucose (18F-FDG), 3′-deoxy-3′-[18F]fluorothymidine (18F-FLT), and [18F]-fluoromisonidazole (18F-FMISO) microPET measurements, as well as kinetic parameters returned from analyses of dynamic 18F-FLT and 18F-FMISO data.Procedures
HER2+ xenografts were established in nude mice. Dynamic data were acquired for 60 min, followed by a repeat injection and second scan 6 h later. Reproducibility was assessed for the percent-injected dose per gram (%ID/g) for each radiotracer, and with kinetic parameters (K 1 –k 4 , K i ) for 18F-FLT and 18F-FMISO.Results
The value needed to reflect a change in tumor physiology is given by the 95 % confidence interval (CI), which is ±14, ±5, and ±6 % for 18F-FDG (n?=?12), 18F-FLT (n?=?11), and 18F-FMISO (n?=?11) %ID/g, respectively. V d (=K 1 /k 2), k 3, and K FLT are the most reproducible 18F-FLT (n?=?9) kinetic parameters, with 95 % CIs of ±18, ±10, and ±18 %, respectively. V d and K FMISO are the most reproducible 18F-FMISO kinetic parameters (n?=?7) with 95 % CIs of ±16 and ±14 %, respectively.Conclusions
Percent-injected dose per gram measurements are reproducible and appropriate for detecting treatment-induced changes. Kinetic parameters have larger threshold values, but are potentially sufficiently reproducible to detect treatment response. 相似文献3.
Virginie Frings Adrianus J. de Langen Maqsood Yaqub Robert C. Schuit Astrid A. M. van der Veldt Otto S. Hoekstra Egbert F. Smit Ronald Boellaard 《Molecular imaging and biology》2014,16(1):136-145
Purpose
To investigate the effect of image-derived input functions (IDIF), input function corrections and volume of interest (VOI) size in quantification of [18F]FLT uptake in non-small cell lung cancer (NSCLC) patients.Procedures
Twenty-three NSCLC patients were scanned on a HR+ scanner. IDIFs were defined over the aorta and left ventricle. Activity concentration and metabolite fraction were measured in venous blood samples. Venous blood samples at 30, 40 and 60 min after injection were used to calibrate the IDIF time–activity curves. Adaptive thresholds were used for VOI definition. Full kinetic analysis and simplified measures were performed.Results
Non-linear regression analysis showed better fits for the irreversible model compared to the reversible model in the majority. Calibrated and metabolite corrected plus plasma-to-blood ratio corrected input function resulted in high correlations between SUV and Patlak K i (Pearson correlation coefficients 0.86–0.96, p value?<?0.001). No significant differences in correlation between SUV and Patlak K i were observed with variation of IDIF structure or VOI size.Conclusions
Plasma-to-blood ratio correction, metabolite correction and calibration improved the correlation between SUV and Patlak K i significantly, indicating the need for these corrections when K i is used to validate semi-quantitative measures, such as SUV. 相似文献4.
Olivia J. Kelada Sara Rockwell Ming-Qiang Zheng Yiyun Huang Yanfeng Liu Carmen J. Booth Roy H. Decker Uwe Oelfke Richard E. Carson David J. Carlson 《Molecular imaging and biology》2017,19(6):893-902
Purpose
The purpose of this study is to use dynamic [18F]fluoromisonidazole ([18F]FMISO) positron emission tomography (PET) to compare estimates of tumor hypoxic fractions (HFs) derived by tracer kinetic modeling, tissue-to-blood ratios (TBR), and independent oxygen (pO2) measurements.Procedures
BALB/c mice with EMT6 subcutaneous tumors were selected for PET imaging and invasive pO2 measurements. Data from 120-min dynamic [18F]FMISO scans were fit to two-compartment irreversible three rate constant (K 1, k 2, k 3) and Patlak models (K i). Tumor HFs were calculated and compared using K i, k 3, TBR, and pO2 values. The clinical impact of each method was evaluated on [18F]FMISO scans for three non-small cell lung cancer (NSCLC) radiotherapy patients.Results
HFs defined by TBR (≥1.2, ≥1.3, and ≥1.4) ranged from 2 to 85 % of absolute tumor volume. HFs defined by K i (>0.004 ml min cm?3) and k 3 (>0.008 min?1) varied from 9 to 85 %. HF quantification was highly dependent on metric (TBR, k 3, or K i) and threshold. HFs quantified on human [18F]FMISO scans varied from 38 to 67, 0 to 14, and 0.1 to 27 %, for each patient, respectively, using TBR, k 3, and K i metrics.Conclusions
[18F]FMISO PET imaging metric choice and threshold impacts hypoxia quantification reliability. Our results suggest that tracer kinetic modeling has the potential to improve hypoxia quantification clinically as it may provide a stronger correlation with direct pO2 measurements.5.
Objective
Prostacyclins have been suggested to exert positive inotropic effects which would render them particularly suitable for the treatment of right ventricular (RV) dysfunction due to acute pulmonary hypertension (PHT). Data on this subject are controversial, however, and vary with the experimental conditions. We studied the inotropic effects of epoprostenol at clinically recommended doses in an experimental model of acute PHT.Design and setting
Prospective laboratory investigation in a university hospital laboratory.Subjects
Six pigs (36?±?7?kg).Interventions
Pigs were instrumented with biventricular conductance catheters, a pulmonary artery (PA) flow probe, and a high-fidelity pulmonary pressure catheter. Incremental doses of epoprostenol (10, 15, 20, 30, 40?ng?kg–1?min–1) were administered in undiseased animals and after induction of acute hypoxia-induced PHT.Measurements and results
In acute PHT epoprostenol markedly reduced RV afterload (slopes of pressure-flow relationship in the PA from 7.0?±?0.6 to 4.2?±?0.7?mmHg?min?l–1). This was associated with a paradoxical and dose-dependent decrease in RV contractility (slope of preload-recruitable stroke-work relationship from 3.0?±?0.4 to 1.6?±?0.2?mW?s?ml–1; slope of endsystolic pressure-volume relationship from 1.5?±?0.3 to 0.7?±?0.3?mmHg?ml–1). Left ventricular contractility was reduced only at the highest dose. In undiseased animals epoprostenol did not affect vascular tone and produced a mild biventricular decrease in contractility.Conclusions
Epoprostenol has no positive inotropic effects in vivo. In contrast, epoprostenol-induced pulmonary vasodilation in animals with acute PHT was associated with a paradoxical decrease in RV contractility. This effect is probably caused indirectly by the close coupling of RV contractility to RV afterload. However, data from normal animals suggest that mechanisms unrelated to vasodilation are also involved in the observed negative inotropic response to epoprostenol. 相似文献6.
Jonathan T. Elliott Kenneth M. Tichauer Kimberley S. Samkoe Jason R. Gunn Kristian J. Sexton Brian W. Pogue 《Molecular imaging and biology》2014,16(4):488-494
Purpose
With the goal of facilitating tracer kinetic analysis in small-animal planar fluorescence imaging, an experimental method for characterizing tracer arterial input functions is presented. The proposed method involves exposing the common carotid arteries by surgical dissection, which can then be imaged directly during tracer injection and clearance.Procedures
Arterial concentration curves of IRDye-700DX-carboxylate, IRDye-800CW-EGF, and IRDye-800CW conjugated to anti-EGFR Affibody are recovered from athymic female mice (n?=?12) by directly imaging exposed vessels. Images were acquired with two imaging protocols: a slow-kinetics approach (temporal resolution?=?45 s) to recover the arterial curves from two tracers simultaneously, and a fast-kinetics approach (temporal resolution?=?500 ms) to characterize the first-pass peak of a single tracer. Arterial input functions obtained by the carotid imaging technique, as well as plasma curves measured by blood sampling were fit with a biexponential pharmacokinetic model.Results
Pharmacological fast- and slow-phase rate constants recovered with the proposed method were 0.37?±?0.26 and 0.007?±?0.001 min?1, respectively, for the IRDye700DX-C. For the IRDye800CW-EGF, the rate constants were 0.11?±?0.13 and 0.003?±?0.002 min?1. These rate constants did not differ significantly from those calculated previously by blood sampling, as determined by an F test; however, the between-subject variability was four times lower for arterial curves recovered using the proposed technique, compared with blood sampling.Conclusions
The proposed technique enables the direct characterization of arterial input functions for kinetic analysis. As this method requires no additional instrumentation, it is immediately deployable in commercially available planar fluorescence imaging systems. 相似文献7.
Stéphane Guillouet Delphine Patin Olivier Tirel Jérôme Delamare Fabienne Gourand Jean Bernard Deloye Michel Leporrier Louisa Barré 《Molecular imaging and biology》2014,16(1):28-35
Purpose
An efficient and fully automated radiosynthesis of 2-[18F]fluoro-9-β-d-arabinofuranosyl-adenine (2-[18F]fludarabine, [18F]-5) based on a GE TRACERlab? FX-FN module has been developed.Procedures
A 2-nitro purine derivative 3 was developed as precursor for labeling with fluorine-18. The radiosynthesis of [18F]-5 was performed in two steps in a single reactor with an intermediary purification on Sep-Pak® silica which involved the addition of a three-way valve on the original module. After hydrolysis, [18F]-5 was purified by semi-preparative high-pressure liquid chromatography (HPLC) and a quality control was established.Results
The labeling precursor 3 was obtained in 45 % overall yield. Nucleophilic substitution with K18F/K2.2.2 afforded protected 2-[18F]fludarabine ([18F]-4) in 73?±?4 % , radiochemical yield (decay corrected to the end of bombardment (EOB)) and based on the initial [18F]F? activity. An aqueous ammonia/methanol solution was used for the deprotection reaction and gave the desired [18F]-5 in 67?±?3 % yield after 20 min at 70 °C based on HPLC profile.Conclusions
The process afforded pure 2-[18F]fludarabine in 48?±?3 % yield (decay corrected to the EOB) in 85 min, with a specific activity of 310?±?72 GBq/μmol at the end of synthesis (EOS) and a radiochemical purity up to 99 %. 相似文献8.
Antonella Marangoni Cristina Nanni Carmelo Quarta Claudio Foschi Incoronata Russo Paola Nardini Antonietta D’Errico Francesca Rosini Alice Ferretti Rita Aldini Roberto Cevenini Domenico Rubello 《Molecular imaging and biology》2013,15(4):450-455
Purpose
The aim of this study is to explore the feasibility of 11C-Choline PET in the assessment of the degree of inflammation in the Chlamydia muridarum genital infection model.Procedures
Forty female Balb/c mice received 2.5 mg of medroxyprogesterone acetate i.m. 9 and 2 days prior to the infection: 21 mice were infected by C. muridarum into the vaginal vault, 12 mice were treated with inactivated chlamydiae, and 7 mice were SPG buffer-treated as negative controls. Three healthy control mice were not treated with progesterone. Mice in each category were randomly subdivided in two groups: (1) sacrificed at 5, 10, 15, and 20 days for histological analysis and (2) undergoing 11C-Choline PET at days 5, 10, and 20 post-infection (20 MBq of 11C-Choline, uptake time of 10 min, acquisition through a small-animal PET tomograph for 15 min).Results
Infected animals showed a significantly higher standardized uptake value than both controls and animals inoculated with heat-inactivated chlamydiae in each PET scan (P?<?0.05). All organs of the infected animals had scores of inflammation ranging between 2 and 3 at day 5, decreasing to 1–2 at day 20.Conclusions
This preliminary result demonstrated that 11C-Choline PET can highlight a specific proliferation mechanism of inflammatory cells induced by C. muridarum, thanks to a very high sensitivity in detecting very small amounts of tracer in inflammatory cells. 相似文献9.
Mei Tian Wei Lu Rui Zhang Chiyi Xiong Joe Ensor Javier Nazario James Jackson Colette Shaw Katherine A. Dixon Jennifer Miller Kenneth Wright Chun Li Sanjay Gupta 《Molecular imaging and biology》2013,15(5):614-624
Purpose
This study was designed to investigate the intratumoral uptake of hollow gold nanospheres (HAuNS) after hepatic intra-arterial (IA) and intravenous (IV) injection in a liver tumor model.Materials and methods
Fifteen VX2 tumor-bearing rabbits were randomized into five groups (n?=?3 in each group) that received either IV 64Cu-labeled PEG-HAuNS (IV-PEG-HAuNS), IA 64Cu-labeled PEG-HAuNS (IA-PEG-HAuNS), IV cyclic peptide (RGD)-conjugated 64Cu-labeled PEG-HAuNS (IV-RGD-PEG-HAuNS), IA RGD-conjugated 64Cu-labeled PEG-HAuNS (IA-RGD-PEG-HAuNS), or IA 64Cu-labeled PEG-HAuNS with lipiodol (IA-PEG-HAuNS-lipiodol). The animals underwent PET/CT 1 h after injection, and uptake expressed as percentage of injected dose per gram of tissue (%ID/g) was measured in tumor and major organs. The animals were euthanized 24 h after injection, and tissues were evaluated for radioactivity.Results
At 1 h after injection, animals in the IA-PEG-HAuNS-lipiodol group showed significantly higher tumor uptake (P?<?0.001) and higher ratios of tumor-to-normal liver uptake (P?<?0.001) than those in all other groups. The biodistribution of radioactivity 24 h after injection showed that IA delivery of PEG-HAuNS with lipiodol resulted in the highest tumor uptake (0.33 %ID/g; P?<?0.001) and tumor-to-normal liver ratio (P?<?0.001) among all delivery methods. At 24 h, the IA-RGD-PEG-HAuNS group showed higher tumor uptake than the IA-PEG-HAuNS group (0.20 vs. 0.099 %ID/g; P?<?0.001).Conclusion
Adding iodized oil to IA-PEG-HAuNS maximizes nanoparticle delivery to hepatic tumors and therefore may be useful in targeted chemotherapy and photoablative therapy. PET/CT can be used to noninvasively monitor the biodistribution of radiolabeled HAuNS after IV or IA injection. 相似文献10.
Anwar AM Soliman OI Nemes A Geleijnse ML ten Cate FJ 《The international journal of cardiovascular imaging》2008,24(1):45-52
Methods
The study included 25 hypertrophic cardiomyopathy (HCM) patients (15 non-obstructive and 10 obstructive) and 25 controls for assessment of left atrial (LA) volume, mass and function by two-dimensional echocardiography. Measurement included mean LA diameter (LAD), LA mass = {(mean LAD + anterior LA wall + posterior LA wall)3 ? mean LAD3} × 0.8 + 0.6, LA volume = [(8/3 π L · A1 · A2), where L is LA length, A1 and A2 are LA area in 4-chambers and 2-chambers, respectively] including maximum (V max), minimum (V min), and pre-atrial contraction (V pre-A), total atrial stroke volume (TA-SV), TA emptying fraction (TA-EF), active atrial SV (AA-SV), AA-EF, passive atrial SV (PA-SV), PA-EF, atrial expansion index (AEI), and LA kinetic energy (LA-KE) = ½ × AA-SV × P × V2.Results
LAD, LA mass, V max, V min, and V pre-A were significantly higher in HCM than controls. TA-SV and TA-EF were comparable in both HCM subgroups and controls. AA-SV and LA-KE were significantly higher in both HCM subgroups than controls. LA-KE was significantly higher in obstructive HCM than non-obstructive (P < 0.001). PA-EF and AEI were significantly lower in obstructive HCM than controls (P < 0.05).Conclusion
HCM is associated with increased LA size and augmented LA pump function especially obstructive type. LA conduit and reservoir functions are impaired in obstructive HCM. 相似文献11.
Qin Lu Cassio Girardi Mao Zhang Belaïd Bouhemad Kamel Louchahi Olivier Petitjean Frédéric Wallet Marie-Helene Becquemin Gilles Le Naour Charles-Hugo Marquette Jean-Jacques Rouby 《Intensive care medicine》2010,36(7):1147-1155
Purpose
Emergence of multidrug-resistant strains in intensive care units has renewed interest in colistin, which often remains the only available antimicrobial agent active against resistant Pseudomonas aeruginosa. The aim of this study is to compare lung tissue deposition and antibacterial efficiency between nebulized and intravenous administration of colistin in piglets with pneumonia caused by P. aeruginosa.Methods
In ventilated piglets, colistimethate was administered 24 h following bronchial inoculation of Pseudomonas aeruginosa (minimum inhibitory concentration of colistin = 2 μg ml?1) either by nebulization (8 mg kg?1 every 12 h, n = 6) or by intravenous infusion (3.2 mg kg?1 every 8 h, n = 6). All piglets were killed 49 h after inoculation. Colistin peak lung tissue concentrations and lung bacterial burden were assessed on multiple post mortem subpleural lung specimens.Results
Median colistin peak lung concentration following nebulization was 2.8 μg g?1 (25–75% interquartile range = 0.8–13.7 μg g?1). Colistin was undetected in lung tissue following intravenous infusion. In the aerosol group, peak lung tissue concentrations were significantly greater in lung segments with mild pneumonia (median = 10.0 μg g?1, 25–75% interquartile range = 1.8–16.1 μg g?1) than in lung segments with severe pneumonia (median = 1.2 μg g?1, 25–75% interquartile range = 0.5–3.3 μg g?1) (p < 0.01). After 24 h of treatment, 67% of pulmonary segments had bacterial counts <102 cfu g?1 following nebulization and 28% following intravenous administration (p < 0.001). In control animals, 12% of lung segments had bacterial counts <102 cfu g?1 49 h following bronchial inoculation.Conclusion
Nebulized colistin provides rapid and efficient bacterial killing in ventilated piglets with inoculation pneumonia caused by Pseudomonas aeruginosa. 相似文献12.
Prashanth K. Padakanti Xiang Zhang Hongjun Jin Jinquan Cui Ruike Wang Junfeng Li Hubert P. Flores Stanley M. Parsons Joel S. Perlmutter Zhude Tu 《Molecular imaging and biology》2014,16(6):773-780
Purpose
The vesicular acetylcholine transporter (VAChT) is a specific biomarker for imaging presynaptic cholinergic neurons. Herein, two potent and selective 11C-labeled VAChT inhibitors were evaluated in rodents and nonhuman primates for imaging VAChT in vivo.Procedures
For both (?)-[11C]2 and (?)-[11C]6, biodistribution, autoradiography, and metabolism studies were performed in male Sprague Dawley rats. Positron emission tomography (PET) brain studies with (?)-[11C]2 were performed in adult male cynomolgus macaques; 2 h dynamic data was acquired, and the regions of interest were drawn by co-registration of the PET images with the MRI.Results
The resolved enantiomers (?)-2 and (?)-6 were very potent and selective for VAChT in vitro (K i ?5 nM for VAChT with >35-fold selectivity for VAChT vs. σ receptors); both radioligands, (?)-[11C]2 and (?)-[11C]6, demonstrated high accumulation in the VAChT-enriched striatum of rats. (?)-[11C]2 had a higher striatum to cerebellum ratio of 2.4-fold at 60 min; at 30 min, striatal uptake reached 0.550?±?0.086 %ID/g. Uptake was also specific and selective; following pretreatment with (±)-2, striatal uptake of (?)-[11C]2 in rats at 30 min decreased by 50 %, while pretreatment with a potent sigma ligand had no significant effect on striatal uptake in rats. In addition, (?)-[11C]2 displayed favorable in vivo stability in rat blood and brain. PET studies of (?)-[11C]2 in nonhuman primates indicate that it readily crosses the blood-brain barrier (BBB) and provides clear visualization of the striatum; striatal uptake reaches the maximum at 60 min, at which time the target to nontarget ratio reached ~2-fold.Conclusions
The radioligand (?)-[11C]2 has high potential to be a suitable PET radioligand for imaging VAChT in the brain of living subjects. 相似文献13.
Lina Y. Hu Nadine Bauer Leah M. Knight Zibo Li Shuanglong Liu Carolyn J. Anderson Peter S. Conti Julie L. Sutcliffe 《Molecular imaging and biology》2014,16(4):567-577
Purpose
The integrin αvβ6 is overexpressed in a variety of aggressive cancers and serves as a prognosis marker. This study describes the conjugation, radiolabeling, and in vitro and in vivo evaluation of four chelators to determine the best candidate for 64Cu radiolabeling of A20FMDV2, an αvβ6 targeting peptide.Procedures
Four chelators were conjugated onto PEG28-A20FMDV2 (1): 11-carboxymethyl-1,4,8,11-tetraazabicyclo[6.6.2]hexadecane-4-methanephosphonic acid (CB-TE1A1P), 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA), 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA), and 4,4′-((3,6,10,13,16,19-hexazazbicyclo[6.6.6]ico-sane-1,8-diylbis(aza-nediyl))bis(methylene)dibenzoic acid (BaBaSar). All peptides were radiolabeled with 64Cu in ammonium acetate buffer at pH 6 and formulated to pH 7.2 in PBS for use. The radiotracers were evaluated using in vitro cell binding and internalization assays and serum stability assays. In vivo studies conducted include blocking, biodistribution, and small animal PET imaging. Autoradiography and histology were also conducted.Results
All radiotracers were radiolabeled in good radiochemical purity (>95 %) under mild conditions (37–50 °C for 15 min) with high specific activity (0.58–0.60 Ci/μmol). All radiotracers demonstrated αvβ6-directed cell binding (>46 %) with similar internalization levels (>23 %). The radiotracers 64Cu-CB-TE1A1P-1 and 64Cu-BaBaSar-1 showed improved specificity for the αvβ6 positive tumor in vivo over 64Cu-DOTA-1 and 64Cu-NOTA-1 (+/? tumor uptake ratios—3.82?+/-?0.44, 3.82?±?0.41, 2.58?±?0.58, and 1.29?±?0.14, respectively). Of the four radiotracers, 64Cu-NOTA-1 exhibited the highest liver uptake (10.83?±?0.1 % ID/g at 4 h).Conclusions
We have successfully conjugated, radiolabeled, and assessed the four chelates CB-TE1A1P, DOTA, NOTA, and BaBaSar both in vitro and in vivo. However, the data suggests no clear “best candidate” for the 64Cu-radiolabeling of A20FMDV2, but instead a trade-off between the different properties (e.g., stability, selectivity, pharmacokinetics, etc.) with no obvious effects of the individual chelators. 相似文献14.
Justin C. Brown Lilie L. Lin Saya Segal Christina S. Chu Ashley E. Haggerty Emily M. Ko Kathryn H. Schmitz 《Supportive care in cancer》2014,22(11):3017-3025
Purpose
We sought to quantify the proportion of uterine cancer survivors who self-report poor physical function. We then sought to quantify the association of poor physical function with physical activity (PA), walking, and lower limb lymphedema (LLL), among women with a history of uterine cancer.Methods
Physical function was quantified using the Medical Outcomes Study 12-Item Short-Form Health Survey (SF-12) questionnaire. PA, walking, and LLL were measured using self-report questionnaire. PA was calculated using metabolic equivalent hours per week (MET-h week?1), and walking was calculated using blocks per day (blocks day?1). Logistic regression estimated odds ratios (OR) and 95 % confidence intervals (95 % CI).Results
Among the 213 uterine cancer survivors in our survey (43 % response rate), 35 % self-reported poor physical function. Compared to participants who reported <3.0 MET-h week?1 of PA, participants who reported ≥18.0 MET-h week?1 of PA were less likely to have poor physical function (OR 0.03, 95 % CI 0.01–0.10; P trend ?0.0001). Compared to participants who reported <4.0 blocks day?1 of walking, participants who reported ≥12.0 blocks day?1 of walking were less likely to have poor physical function (OR 0.07, 95 % CI 0.03–0.19; P trend ?0.0001). Compared to participants who did not have LLL, participants with LLL were more likely to have poor physical function (OR 5.25, 95 % CI 2.41–11.41; P?0.0001).Conclusion
Higher levels of PA and walking associate with a lower likelihood of reporting poor physical function. The presence of LLL associates with a higher likelihood of reporting poor physical function. These findings are hypothesis-generating and should be evaluated in future prospective studies. 相似文献15.
Ryuichi Harada Nobuyuki Okamura Shozo Furumoto Takeo Yoshikawa Hiroyuki Arai Kazuhiko Yanai Yukitsuka Kudo 《Molecular imaging and biology》2014,16(1):19-27
Purpose
Selective visualization of amyloid-β and tau protein deposits will help to understand the pathophysiology of Alzheimer’s disease (AD). Here, we introduce a novel fluorescent probe that can distinguish between these two deposits by multispectral fluorescence imaging technique.Procedures
Fluorescence spectral analysis was performed using AD brain sections stained with novel fluorescence compounds. Competitive binding assay using [3H]-PiB was performed to evaluate the binding affinity of BF-188 for synthetic amyloid-β (Aβ) and tau fibrils.Results
In AD brain sections, BF-188 clearly stained Aβ and tau protein deposits with different fluorescence spectra. In vitro binding assays indicated that BF-188 bound to both amyloid-β and tau fibrils with high affinity (K i ?<?10 nM). In addition, BF-188 showed an excellent blood–brain barrier permeability in mice.Conclusion
Multispectral imaging with BF-188 could potentially be used for selective in vivo imaging of tau deposits as well as amyloid-β in the brain. 相似文献16.
17.
Yuping Xu Donghui Pan Chen Zhu Qing Xu Lizhen Wang Fei Chen Runlin Yang Shineng Luo Min Yang Yongjun Yan 《Molecular imaging and biology》2014,16(4):578-585
Purpose
Follicle-stimulating hormone receptor (FSHR) is overexpressed in primary and metastatic tumor. Molecular imaging of FSHR is beneficial for prognosis and therapy of cancer. FSHβ(33–53) (YTRDLVYKDPARPKIQKTCTF), denoted as FSH1, is a FSHR antagonist. In the present study, maleimide-NOTA conjugate of FSH1 (NOTA-MAL-FSH1) was designed and labeled with [18F] aluminum fluoride. The resulting tracer, 18F-Al-NOTA-MAL-FSH1, was preliminarily evaluated in PET imaging of FSHR-positive tumor.Procedures
NOTA-MAL-FSH1 was synthesized and radiolabeled with Al18F complex. The tumor-targeting potential and pharmacokinetic profile of the 18F-labeled compound were evaluated in vitro and in vivo using a PC3 human prostate tumor model.Results
18F-Al-NOTA-MAL-FSH1 can be efficiently produced within 30 min with a non-decay-corrected yield of 48.6?±?2.1 % and a radiochemical purity of more than 95 %. The specific activity was at least 30 GBq/μmol. The radiotracer was stable in phosphate-buffered saline and human serum for at least 2 h. The IC50 values of displacement 18F-Al-NOTA-MAL-FSH1 with FSH1 were 252?±?1.12 nM. The PC3 human prostate tumor xenografts were clearly visible with high contrast after injection of 18F-Al-NOTA-MAL-FSH1 via microPET. At 30, 60 and 120 min postinjection, the tumor uptakes were 2.98?±?0.29 % injected dose (ID)/g, 2.53?±?0.20 %ID/g and 1.36?±?0.12 %ID/g, respectively. Dynamic PET scanning showed that tumor uptake reached a plateau by about 6 min. Heart peaked earlier and then cleared quickly. Biodistribution studies confirmed that the normal organs except kidney uptakes were all below 1 %ID/g at 1 h p.i. The tumor-to-blood and tumor-to-muscle ratio at 10 min, 0.5, 1, and 2 h after injection were 1.64?±?0.36, 2.97?±?0.40, 9.31?±?1.06, and 13.59?±?2.33 and 7.05?±?1.10, 10.10?±?1.48, 16.17?±?3.29, and 30.88?±?4.67, respectively. The tracer was excreted mainly through the renal system, as evidenced by high levels of radioactivity in the kidneys. FSHR-binding specificity was also demonstrated by reduced tumor uptake of 18F-Al-NOTA-MAL-FSH1 after coinjection with an excess of unlabeled FSH1 peptide.Conclusion
NOTA-MAL-FSH1 could be labeled rapidly and efficiently with 18F using one step method. Favorable preclinical data suggest that 18F-Al-NOTA-MAL-FSH1 may be a suitable radiotracer for the non-invasive visualization of FSHR positive tumor in vivo. 相似文献18.
Andrei Todica Stefan Brunner Guido Böning Sebastian Lehner Stephan G. Nekolla Moritz Wildgruber Christopher Übleis Carmen Wängler Martina Sauter Karin Klingel Paul Cumming Peter Bartenstein Ralf Schirrmacher Wolfgang Michael Franz Marcus Hacker 《Molecular imaging and biology》2013,15(4):441-449
Purpose
The purpose of this study is to evaluate left ventricular functional parameters in healthy mice and in different murine models of cardiomyopathy with the novel blood pool (BP) positron emission tomography (PET) tracer [68Ga]-albumin.Procedures
ECG-gated microPET examinations were obtained in healthy mice, and mice with dilative (DCM) and ischemic cardiomyopathy (ICM) using the novel BP tracer [68Ga]-albumin (AlbBP), as well as [18F]-FDG microPET. Cine-magnetic resonance imaging (MRI) examination performed on a clinical 1.5-T MRI provided the reference standard measurements.Results
When considering the combined group of healthy controls, DCM and ICM AlbBP-PET significantly overestimated the magnitudes of EDV (AlbBP, 181?±?86 μl; cine-MRI, 125?±?80 μl; P?<?0.001) and ESV (AlbBP, 136?±?92 μl; cine-MRI, 96?±?77 μl; P?<?0.001), whereas the EF (AlbBP, 31?±?16 %; cine-MRI, 33?±?21 %; P?=?0.910) matched closely to cine-MRI results, as did findings with [18F]-FDG. High correlations were found between the measured cardiac parameters (EDV: R?=?0.978, ESV: R?=?0.989, and LVEF: R?=?0.992).Conclusions
Measuring left ventricular function in mice with [68Ga]-albumin BP PET is feasible and showed a high correlation compared to cine-MRI, which was used as a reference standard. 相似文献19.
Franck Sobrio Marie Médoc Ludovic Martial Jérôme Delamare Louisa Barré 《Molecular imaging and biology》2013,15(1):12-18
Purpose
[18F]ML-10 is the most advanced radiopharmaceutical for the clinical imaging of the apoptosis phenomenon by PET. The preparation of this radiopharmaceutical on a commercial radiosynthesis module and the requested quality controls for its release are presented herein.Procedures
ML-10 as reference and its mesyloxy derivative as precursor for labelling with fluorine-18 were prepared. [18F]ML-10 was synthesized via a [18F]fluorine-de-mesyloxy aliphatic nucleophilic substitution via a GE TRACERLab® FX-FN module. Quality controls were performed.Results
The labelling precursor was obtained in a four step synthesis in 28 % overall yield affording ML-10 in two steps (88 % yield). Pure [18F]ML-10 was obtained with a decay corrected yield of 39.8 %?±?8.4 % (n?=?7) in 70 min and a specific activity of 235?±?85 GBq/μmol at the end of synthesis.Conclusions
[18F]ML-10 was prepared on a widely available automated module and passed the quality control. A LC/MS method was developed to measure specific radioactivity. 相似文献20.
Giovanna Guiotto Mario Masarone Fiorella Paladino Enrico Ruggiero Sean Scott Sossio Verde Fernando Schiraldi 《Intensive care medicine》2010,36(4):692-696