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1.
背景:近年来研究表明,Toll样受体和核因子κB与白血病的发生发展关系密切。蝎毒对肿瘤细胞表面Toll样受体和细胞内核因子κB的影响还未见文献报道。目的:观察蝎素组分Ⅲ对人白血病单核细胞系THP1细胞Toll样受体4和p65表达的影响。方法:将THP1细胞接种于24孔细胞培养板中,实验分5组:对照组、脂多糖(终浓度为1mg/L)组及不同浓度蝎素组分Ⅲ处理组(脂多糖+蝎素组分Ⅲ终浓度为1,10,20mg/L)。Real-timePCR法检测THP1细胞Toll样受体4和p65mRNA的表达;Westernblot检测Toll样受体4和p65蛋白的表达。结果与结论:脂多糖组THP1细胞Toll样受体4、p65mRNA和蛋白的表达均显著高于对照组(P<0.01);不同浓度蝎素组分Ⅲ处理组Toll样受体4、p65mRNA和蛋白表达均低于脂多糖组(P<0.01),并呈剂量依赖性。提示蝎素组分Ⅲ能有效下调脂多糖诱导的THP1细胞中TLR4的表达,并可有效抑制核因子κB的活性,从而抑制细胞的增殖。  相似文献   

2.
为研究和比较人骨髓间充质干细胞 (MSC)和脐血单个核细胞在体外的免疫调控能力 ,从人骨髓分离培养间充质干细胞 ,用Ficoll分离得到脐血单个核细胞 ,分别以不同剂量加入到外周血混合淋巴细胞培养体系和植物血凝素 (PHA)刺激的外周血淋巴细胞转化体系中 ,用MTT还原法测定细胞增殖 ,分别观察MSC和脐血单个核细胞对混合淋巴细胞反应和淋巴细胞转化的影响。结果显示 ,5× 10 4 ,1× 10 4 MSC以及 2× 10 5脐血单个核细胞均抑制混合淋巴细胞反应和PHA诱导的淋巴细胞增殖 ,而 <1× 10 4 MSC和 <2× 10 5脐血单个核细胞对混合淋巴细胞反应和PHA诱导的淋巴细胞增殖的抑制作用均不明显 ;5× 10 4 MSC对淋巴细胞增殖的抑制能力明显强于 2× 10 5脐血单个核细胞。结论 :大剂量的骨髓间充质干细胞和脐血单个核细胞在体外对混合淋巴细胞反应和PHA诱导的淋巴细胞转化均具有抑制作用 ,而且间充质干细胞的抑制能力强于脐血单个核细胞。  相似文献   

3.
背景:研究证实全髋关节置换后松动假体表面生物膜中表达Toll样受体,但其变化规律尚不清楚.目的:观察关节假体置入患者外周血白细胞Toll样受体2,4的表达.方法:选择关节置换患者和同期10例行关节镜检查患者,于入院次日和假体置入后第3天早晨空腹抽取肘静脉血,流式细胞术分析外周血白细胞中Toll样受体2和Toll样受体4的阳性表达,同时送血样至检验科检查白细胞、血沉、C-反应蛋白水平.结果与结论:两组白细胞、血沉、C-反应蛋白均在正常范围内.Toll样受体2,4均主要表达于外周血单核细胞,而在外周血淋巴细胞和中性粒细胞的表达率较低;关节置换组术后外周血单核细胞Toll样受体2阳性表达率明显低于术前(P < 0.05);关节置换组Toll样受体4、关节镜组Toll样受体2,4手术前后阳性表达率差异无显著性意义(P > 0.05).关节置换组手术前后外周血单核细胞Toll样受体2阳性表达率低于关节镜组(P < 0.05),两组Toll样受体4表达率无差异.提示在无感染的情况下,手术本身应激和局部损伤不影响Toll样受体2,4的表达,假体的置入下调了外周血Toll样受体2的表达.  相似文献   

4.
目的:细胞表面Toll样受体对激发先天性免疫、抵抗微生物感染有重要作用。最近研究发现激活细胞表面Toll样受体不仅可以预防微生物感染,还可以促进组织再生。实验通过脂多糖激活体外培养的角质细胞株Hacat细胞表面Toll样受体,观察其促进创面愈合的可能机制。 方法:实验于2007-07在解放军第四军医大学西京医院烧伤与皮肤外科完成。①实验分组及方法:角质细胞株Hacat由解放军第四军医大学西京医院皮肤科馈赠;脂多糖为Sigma公司产品;小鼠抗入Toll样受体2单克隆抗体、兔抗人Toll样受体4多克隆抗体购于ebioscience公司。体外培养Hacat细胞,根据脂多糖(500ng/L)与Hacat细胞共培养24,48,72h将细胞随机分为脂多糖作用24h组、脂多糖作用48h组、脂多糖作用72h组,另设对照组,只加溶剂二甲基亚枫。②实验评估:蛋自免疫印迹技术检测脂多糖对Hacat细胞Toll样受体2、Toll样受体4及核因子KB表达的影响:荧光定量聚合酶链反应检测脂多糖作用后Hacat细胞内基质金属蛋白酶3、基质金属蛋白酶9及血管内皮生长因子的表达。 结果:光学显微镜下正常入皮肤表皮及角质细胞Toll样受体2、Toll样受体4表达呈蓝黑色:脂多糖作用于Hacat细胞24,48,72h后Toll样受体2、Toll样受体4、核因子-κB表达均高于对照组(P〈0.01)。与对照组相比,其他3组基质金属蛋白酶3及血管内皮生长因子表达均增高(P〈0.01,P〈0.05)。脂多糖作用24h组基质金属蛋白酶9表达与对照组差异无显著性(P〉0.05),脂多糖作用48,72h组与对照组比较,差异显著(P〈0.05)。 结论:激活Hacat细胞表面Toll样受体有促进创面愈合作用,其机制可能与脂多糖作用了:Toll样受体从而激活核因子KB信号传导通路促进细胞释放基质金属蛋白酶3、基质金属蛋白酶9及血管内皮生长因子等因素有关?  相似文献   

5.
背景:研究证实全髋关节置换后松动假体表面生物膜中表达Toll样受体,但其变化规律尚不清楚。目的:观察关节假体置入患者外周血白细胞Toll样受体2,4的表达。方法:选择关节置换患者和同期10例行关节镜检套患者,于入院次日和假体置入后第3天早晨空腹抽取肘静脉血,流式细胞术分析外周血白细胞中Toll样受体2和Toll样受体4的阳性表达,同时送血样至枪验科检查A细胞、血沉、C-反应蛋白水平。结果与结论:两组白细胞、血沉、C-反应蛋白均在正常范围内。Toll样受体2,4均主要表达于外周血单核细胞,而在外周血淋巴细胞和中性粒细胞的表达率较低;关节置换组术后外周血单核细胞Toll样受体2阳性表达率明显低于术前(P〈O.05);关节筐换组Toll样受体4、关节镜组Toll样受体2,4手术前后阳性表达率差异无显著性意义(P〉0.05)。关节置换组手术前后外周血单核细胞Toll样受体2阳性表达率低于关节镜组(P〈0.05),两组Toll样受体4表达率无差异。提示在无感染的情况下,手术本身应激和局部损伤不影响Toll样受体2,4的表达,假体的置入下调了外阍血Toll样受体2的表达。  相似文献   

6.
背景:滑膜组织中可分离出具有多向分化潜能干细胞特性的细胞。目的:探索滑膜间充质干细胞体外分离、培养的可行性、免疫表型的鉴定及对混合淋巴细胞反应体系中淋巴细胞增殖的影响,评价其免疫学特性。方法:关节镜下获取10例半月板损伤患者的膝关节滑膜组织,胶原酶消化获得有核细胞。挑选单细胞克隆,筛选获得滑膜间充质干细胞。检测其增殖能力、细胞活力,流式检测其细胞免疫表型,采用人双向混合淋巴细胞反应体系及植物血凝素刺激的淋巴细胞增殖反应体系,根据滑膜间充质干细胞与外周血单个核细胞的不同比例加入丝裂霉素处理滑膜间充质干细胞。MTT法检测并计算其抑制率。结果与结论:10组滑膜间充质干细胞系增殖能力和细胞活力差异无显著性意义(P>0.05)。10组细胞系免疫表型:CD44、CD90、CD105呈阳性,CD14、CD34、CD45和HLA-DR呈阴性。滑膜间充质干细胞抑制混合淋巴细胞反应体系中及植物血凝素刺激的淋巴细胞增殖反应体系中淋巴细胞的增殖、抑制率与加入的滑膜间充质干细胞数量成正比。提示关节滑膜组织可以分离、培养获得滑膜间充质干细胞,其具有间充质干细胞特异性表型,且滑膜间充质干细胞具有免疫调节作用。  相似文献   

7.
背景:目前对关节置换后机体内Toll样受体变化和相关机制研究甚少,有研究证实全髋关节置换后松动假体表面生物膜中表达Toll样受体。目的:观察关节假体置入后Toll样受体4,9在关节置换者外周血白细胞上的表达。方法:实验组选择11例行关节置换患者,对照组为10例行关节镜检查患者,于入院次日和术后第3天早晨空腹采血,流式细胞术分析外周血白细胞中Toll样受体4,9的阳性表达,同时送血样至检验科检查白细胞计数、血细胞沉降率、C-反应蛋白水平。结果与结论:两组白细胞计数、血细胞沉降率、C-反应蛋白均在正常范围内。Toll样受体4,9均主要表达于外周血单核细胞,而在外周血淋巴细胞和中性粒细胞的表达率较低;两组患者干预后Toll样受体4,9在外周血单核细胞阳性表达率较置换前明显降低(P〉0.05)。实验组患者Toll样受体9置换前后外周血单核细胞阳性表达率的变化明显大于关节镜组(P〈0.05);而Toll样受体4变化差异无显著性意义(P〉0.05)。说明在无感染的情况下,手术本身的应激和局部损伤不影响Toll样受体4,9的表达,假体置入则下调外周血白细胞对Toll样受体9的表达。  相似文献   

8.
背景:目前对关节置换后机体内Toll样受体变化和相关机制研究甚少,有研究证实全髋关节置换后松动假体表面生物膜中表达Toll样受体.目的:观察关节假体置入后Toll样受体4,9在关节置换者外周血白细胞上的表达.方法:实验组选择11例行关节置换患者,对照组为10例行关节镜检查患者,于入院次日和术后第3天早晨空腹采血,流式细胞术分析外周血白细胞中Toll样受体4,9的阳性表达,同时送血样至检验科检查白细胞计数、血细胞沉降率、C-反应蛋白水平.结果与结论:两组白细胞计数、血细胞沉降率、C-反应蛋白均在正常范围内.Toll样受体4,9均主要表达于外周血单核细胞,而在外周血淋巴细胞和中性粒细胞的表达率较低;两组患者干预后Toll样受体4,9在外周血单核细胞阳性表达率较置换前明显降低(P > 0.05).实验组患者Toll样受体9置换前后外周血单核细胞阳性表达率的变化明显大于关节镜组(P < 0.05);而Toll样受体4变化差异无显著性意义(P > 0.05).说明在无感染的情况下,手术本身的应激和局部损伤不影响Toll样受体4,9的表达,假体置入则下调外周血白细胞对Toll样受体9的表达.  相似文献   

9.
目的观察革兰阴性菌感染所致脓毒症患者外周血单个核细胞Toll样受体4(TLR4)的表达与内毒素耐受之间的关系。方法以重症监护病房治疗的32例革兰阴性菌感染所致脓毒症患者为研究对象,另选20例为健康对照组。两组入选人员均晨起空腹采血,分离外周血单个核细胞,采用流式细胞仪技术检测单个核细胞表面TLR4的表达;另一部分外周血分离单个核细胞,并加入1μg/ml脂多糖(LPS)培养24h,同样进行流式细胞仪检测单个核细胞表面TLR4的表达。并应用放射免疫法测定血清及外周血单个核细胞培养液中TNF-α、IL-6的浓度。结果 (1)脓毒症组外周血单个核细胞表面TLR4表达、血清细胞因子TNF-α、IL-6浓度均明显高于健康对照组(均P<0.001)。(2)脓毒症组患者外周血单个核细胞经LPS刺激后培养上清液中TNF-α、IL-6的浓度显著低于健康对照组(均P<0.05),脓毒症组患者外周血单个核细胞经LPS刺激后TLR4表达较刺激前轻度下调,但刺激前后组间无统计学差异(P>0.05)。结论脓毒症患者外周血单个核细胞体外给予大剂量LPS刺激后可以产生内毒素耐受,但这种现象不能单纯用TLR4的表达下调解释,其可能存在更复杂的机制。内毒素耐受状况下,单个核细胞表现为促炎因子表达的明显下调。  相似文献   

10.
背景:胎盘由滋养细胞及大量起源于胚外中胚层的间充质和血管共同组成,提示胎盘中存在间充质干细胞成分.目的:探索胎盘间充质干细胞对混合淋巴细胞反应体系中淋巴细胞增殖和细胞因子分泌的影响,评价其免疫学特性.方法:分离培养胎盘间充质干细胞,采用人以及兔双向混合淋巴细胞反应体系,根据胎盘间充质干细胞:外周血单个核细胞的不同比例(1:5,1:10,1:20,1:50,1:100,1:500)加入丝裂霉素处理胎盘间充质干细胞.3H掺入法测定淋巴细胞增殖率并用 ELISA法测定混合培养上清液中白细胞介素2和γ-干扰索的含量.结果与结论:胎盘间充质于细胞抑制人或兔混合淋巴细胞反应体系中淋巴细胞的增殖,抑制率与加入的胎盘间充质干细胞数量成正比,同时胎盘间充质干细胞减少混合淋巴细胞反应中细胞因了白细胞介素2、γ-干扰素的分泌.提示胎盘间充质于细胞具有免疫调节作用,可以抑制同种异体或异种混合淋巴细胞反应体系中淋巴细胞的增殖.  相似文献   

11.
We examined the sensitivity of lymphocytes from different age groups to inhibition by prostaglandin E2. Phytohemagglutinin-stimulated cultures of peripheral blood mononuclear cells from 12 healthy subjects over the age of 70 were much more sensitive to inhibition by exogenously added prostaglandin E2 than were cells from 17 young controls (ID50 congruent to 10 nM for the subjects over 70 vs. greater than 3 micronM for the young controls). The more senstivie lymphocytes from a subject over 70 were to prostaglandin E2, the lower was his or her response to phytohemagglutinin (r = 0.75, P less than 0.01). The mean responses to phytohemagglutinin of the peripheral blood mononuclear cells from the subjects over 70 were significantly depressed compared to the young controls. Addition of indomethacin, a prostaglandin synthetase inhibitor, to the cultures resulted in an increase in [3H]thymidine incorporation of 140 +/- 16% in the cells of the subjects over 70 vs. a 36 +/- 3% increase in the young controls (mean +/- SEM, P less than 0.001). The mean phytohemagglutinin response of the subjects over 70 was 40% of the control response without indomethacin. With addition of indomethacin the response of subjects over 70 rose to 72% of control. Thus, increased sinsitivity to prostaglandin E2 appears to be responsible in part for the depressed mitogen response of peripheral blood mononuclear cells from healthy subjects over 70.  相似文献   

12.
本研究探讨前列腺素E2(PGE2)对外周血T淋巴细胞体外增殖的影响,以及对Th1/Th2和Tc1/Tc2细胞的免疫平衡的调节作用。不同浓度PGE2与抗CD3和抗CD28单克隆抗体(mAb)和健康成人外周血单个核细胞(MNC)共同培养120小时,测定细胞增殖程度。ELISA方法测定24、48、72和120小时细胞培养上清液中IFN-γ和IL-4浓度变化。流式细胞仪测定CD4+IL-4+T细胞和CD4+1FN-γT细胞以及CD8+IL-4+T细胞和CD8+IFN-γ+ T细胞比值。各实验均以不加PGE2为对照。结果表明:①随PGE:的浓度增加,T细胞体外增殖的抑制率明显增高(p=0.001);T细胞增殖抑制率与PGE2浓度之间呈明显的正相关(r=0.889,P=0.000)。②实验组培养120小时的IFN-γ浓度与第72小时的IFN-γ浓度差异无显著性(P=0.917),对照组细胞培养上清液中的IFN-γ浓度随时间持续增高(P=0.046);实验组不同时间的IFN-γ浓度均明显低于对照组(P〈0.05)。实验组在不同时间产生IL-4浓度无明显变化(P=0.400);对照组24小时细胞培养上清中IL-4浓度高于48、72和120小时(P值分别为0.007、0.003和0.002);实验组细胞培养24小时时IL-4浓度明显低于对照组(P=0.037);实验组细胞培养48、72和120小时与对照组IL-4浓度差异无显著性(P〉0.05)。⑧实验组与对照组CD4+IFN-γT细胞的比例无明显变化(P=0.767);实验组CD4+IL-4+T细胞比例略高于对照组(P=0.051);实验组CD4 IL-4+T细胞与CD4+IFN-γT细胞的比值明显高于对照组(P=0.011)。实验组与对照组CD8+IFN-γT细胞的比例无明显变化(P=O.441);实验组CD8+IL-4+T细胞的比例明显高于对照组(P=0.015);实验组CD8+ IL4+T细胞与CD8+IFN-γT细胞的比值明显高于对照组(P=0.038)。结论:PGE2体外抑制外周血T细胞的增殖;PGE2作用24小时即可抑制IFN-γ和儿4的产生,并且明显影响T细胞1FN-γ的高峰出现,对IFN-γ具有持续性的抑制作用,对见-4的持续性影响并不明显;PGE2使CD4+IL-4+T细胞与CD4+IFN-γT细胞的比值和CD8+IL-4+T细胞与CD8+IFN-γT细胞的比值增加,调节T细胞的免疫反应向Th2/T02方向发展。  相似文献   

13.
Periodontal ligament stem cells (PDLSCs) have great potential for regenerating periodontal ligament tissue, which is involved in attaching teeth to the underlying alveolar bone. Recently, PDLSCs were characterized as having both low immunogenicity and profound immunomodulation abilities. Further, transplanted PDLSCs differentiate into osteoblasts in vivo. In the present study, we investigated the immunological characteristics of osteogenic differentiated PDLSCs. We found that PDLSCs expressed mesenchymal stem cells markers, including STRO‐1 and CD146, but were negative for CD14, CD34 and CD45. RT–PCR indicated that NCAM1, MSX1 and S100A4 were expressed in PDLSCs. The cells underwent osteogenic and adipogenic differentiation when cultured in defined medium. Osteogenic differentiated PDLSCs failed to stimulate allogeneic T cell proliferation and suppressed phytohaemagglutinin‐triggered T cell proliferation. Indomethacin, an inhibitor of prostaglandin E2 (PGE2) production, restored the T cell proliferation inhibited by osteogenic differentiated PDLSCs. These data confirm that osteogenic differentiated PDLSCs have low immunogenicity and demonstrate that they suppress T cell proliferation in vitro through secretion of PGE2. Copyright © 2012 John Wiley & Sons, Ltd.  相似文献   

14.
Purified populations of both human peripheral blood monocytes and murine peritoneal macrophages synthesize and release Prostaglandin E in vitro. In contrast, prostaglandin E was detected in neither the supernate fluids from cultures of highly enriched human lymphocytes and granulocytes, nor in nonadherent murine peritoneal cells. Macrophage prostaglandin E production was markedly enhanced by endotoxin, and completely suppressed by indomethacin. All neoplastic monocyte-macrophage cell lines examined elaborated prostaglandin E in vitro, either constitutively or after induction with endotoxin. In contrast, prostaglandin E production could not be detected from either a T- or B-cell lymphoma, whether or not they were treated with endotoxin. These findings thus indicate that the blood monocyte and tissue macrophage represent an important source of prostaglandin E, a function shared by both normal and neoplastic mononuclear phagocytes.  相似文献   

15.
Human peripheral blood mononuclear cells (lymphocyte-monocyte) in culture release a solube factor which can stimulate, up to 200-fold, production of prostaglandin E2 by isolated, adherent, rheumatoid synovial cells. Production of the factor by the mononuclear cells is enhanced by phytohemagglutinin. This factor is similar in apparent mol vt (10,000-20,000) to that which also stimulates collagenase production by the same cells.  相似文献   

16.
Ciprofloxacin, a fluorinated 4-quinolone, is useful for the clinical treatment of infections due to its antibacterial properties and also modulates the immune response of monocytes isolated from human peripheral blood mononuclear cells. In the present study, we found that ciprofloxacin induced the production of prostaglandin E(2) in monocytes in a concentration-dependent manner regardless of the presence of interleukin-18 by enhancing the expression of cyclooxygenase-2 protein and that this in turn led to the elevation of intercellular cyclic AMP in monocytes via the stimulation of prostaglandin receptors. The prostaglandin E(2) and cyclic AMP production increased by ciprofloxacin was inhibited by indomethacin, a nonselective cyclooxygenase-2 inhibitor, and NS398, a selective cyclooxygenase-2 inhibitor. In addition, ciprofloxacin suppressed the interleukin-18-induced production of tumor necrosis factor alpha, gamma interferon, and interleukin-12 in peripheral blood mononuclear cells by inhibiting the expression of intercellular adhesion molecule 1, B7.1, B7.2, and CD40 on monocytes, and this effect could be reversed by the addition of indomethacin or NS398. These results indicate that ciprofloxacin exerts immunomodulatory activity via the production of prostaglandin E(2) and imply therapeutic potential of ciprofloxacin for the treatment of systemic inflammatory responses initiated by interleukin-18.  相似文献   

17.
In this study we further characterize the properties of the prostaglandin-producing suppressor cell. Overnight preincubation of peripheral blood mononuclear cells results in an increased response of the cells to phytohemagglutinin or Concanavalin A compared to the response of fresh cells. This increase in mitogen response with preincubation was similar in magnitude to the increase in mitogen response of fresh cells after the addition of indomethacin. The two manipulations were not additive; that is, after preincubation, indomethacin caused much less enhancement of mitogen stimulation of peripheral blood mononuclear cells (100 ± 12% increase before preincubation vs. 12 ± 6% after preincubation; mean±SEM, P < 0.001). Preincubated cells also lose sensitivity to inhibition by exogenous prostaglandin E2. It requires the addition of 100- to > 1,000-fold more exogenous PGE2 to produce comparable inhibition of phytohemagglutinin-stimulated preincubated cells than is required for inhibition of phytohemagglutinin-stimulated fresh cells.  相似文献   

18.
The role of immune cell products in modulating connective tissue metabolism was investigated. Supernates of both unstimulated and phytohemagglutinin-stimulated human mononuclear cell cultures suppressed fibroblast proliferation (up to 90%) and concomintantly stimulated fibroblast prostaglandin E(PGE) synthesis (20- to 70-fold). The growth suppression was, at least in part, a secondary result of the increased fibroblast PGE synthesis; growth suppression (a) paralled the increased fibroblast PGE synthesis, (b) was reversed by addition of inhibitors of prostaglandin synthesis (indomethacin, meclofenamate, and eicostaetraynoic acid), and (c) was reproduced by addition of exogenous PGE2 to fibroblast cultures. The prostaglandin-stimulatory, growth-suppressive activity was a product of non-T-lymphocyte, adherent cells and was present within 6 h of mononuclear cell culture. The activity was heat (56 degrees C) and trypsin sensitive, nondialyzable, and appeared in the 12,000-20,000 mol wt fractions by Sephadex G-100 chromatography. The activity in supernates of mononuclear cell cultures was removed by incubation with fibroblasts but not by similar incubation with peripheral blood mononuclear cells. Mononuclear cells release a factor(s) which modulates fibroblast proliferation by altering prostaglandin metabolism.  相似文献   

19.
目的 通过体外实验检测人脐带间充质干细胞的体外多向分化及对犬T淋巴细胞增殖的影响,探讨其作为组织工程种子细胞的可能性.方法 体外培养人脐带间充质干细胞,分别使用含有地塞米松、转化生长因子、3-异丁基-1-甲基黄嘌呤的培养基诱导其成骨、成软骨、成脂肪三系分化后进行染色鉴定,并将经丝裂霉素处理的人脐带间充质干细胞与植物凝集素刺激下的犬外周血T淋巴细胞共培养,5d后多功能酶标仪检测吸光度值.结果 人脐带间充质干细胞体外生长良好,呈长梭形,形态均一,使用成骨、成软骨和成脂培养基分别诱导分化后碱性磷酸酶(ALP)染色、骨钙素免疫细胞化学染色、茜素红染色、亚甲基蓝染色和油红0染色分别呈阳性,人脐带间充质干细胞与犬外周血T淋巴细胞混合组吸光度值较单纯犬外周血T淋巴细胞组吸光度值明显降低.结论 人脐带间充质干细胞具有多向分化能力,并能够抑制犬外周血淋巴细胞的增殖,有望成为创伤组织及骨组织修复的种子细胞.  相似文献   

20.
In previous studies we found that sera from patients with AIDS and AIDS-related disorders inhibit mitogen-stimulated proliferation of peripheral blood mononuclear cells from healthy donors. We have extended these studies to examine the effect of AIDS sera on proliferation of various IL- 2independent, well-characterized T4-positive and T4-negative continuous human lymphoid cell lines. The results suggest that AIDS serum inhibition results from several mechanisms, one involving the induction of prostaglandin E2.  相似文献   

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