热淋清颗粒联合头孢地嗪钠治疗慢性淋菌性前列腺炎的临床效果观察

目的观察热淋清颗粒联合静脉滴注头孢地嗪钠治疗慢性淋菌性前列腺炎的临床效果。方法选取2018年1月至2020年6月河北省衡水市第二人民医院诊治的120例慢性淋菌性前列腺炎患者作为研究对象。随机分为对照组(n=60)和试验组(n=60)。给予对照组单纯静脉滴注头孢地嗪钠治疗,在此基础上,试验组加用热淋清颗粒联合治疗。对比两组治疗效果。结果试验组总有效率高于对照组,差异具有统计学意义(P0.05);两组前列腺液白细胞计数(EPS-WBC)、慢性前列腺炎症状指数(CPSI)评分均较治疗前有显著改善,且试验组患者改善更加明显,差异具有统计学意义(P0.05);两组白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和超敏C-反应蛋白(hs-CRP)均较治疗前均有显著改善,且试验组患者改善更加明显,差异具有统计学意义(P0.05)。结论慢性淋菌性前列腺炎患者采用热淋清颗粒联合静脉滴注头孢地嗪钠进行治疗,临床效果较好,可显著降低患者炎症反应。     

四君子汤加减对复发性尖锐湿疣患者血清IL-2、IL-4水平的影响与疗效观察

目的:通过观察四君子汤加减对复发性尖锐湿疣患者血清白细胞介素4(IL-4)和白细胞介素2(IL-2)的影响,探讨四君子汤加减治疗复发性尖锐湿疣的机理。方法:将80例复发性尖锐湿疣患者经除疣治疗后随机分为治疗组与对照组,治疗组给予中药治疗。检测两组患者治疗前后血清IL-4、IL-2的水平,同时观察两组治疗后疣体的复发情况。结果:治疗组治疗后血清IL-4水平较治疗前降低,且低于治疗后对照组水平,差异均具有统计学意义(P0.05)。治疗组治疗后血清IL-2水平较治疗前升高,且明显高于治疗后对照组的水平,差异均具有统计学意义(P0.05)。治疗后治疗组复发率为17.14%,明显低于对照组,差异具有统计学意义(P0.05)。结论:四君子汤加减能调节机体的细胞免疫功能,可有效防治尖锐湿疣的复发。     

清淋合剂治疗慢性前列腺炎(湿热下注型)的临床疗效研究

目的观察清淋合剂治疗慢性前列腺炎(湿热下注型)的临床疗效。方法选取2017年3月至2017年9月重庆市中医院泌尿外科诊治的慢性前列腺炎(湿热下注型)患者72例作为研究对象。随机分为治疗组(清淋合剂组)和对照组(八正散组),两组疗程为4周,观察两组患者治疗前后美国国立卫生研究院慢性前列腺炎症状评分(NIH-CPSI)、中医症状评分及前列腺液常规的变化。结果治疗组总有效率80.6%,对照组总有效率66.7%;两组患者治疗前后的前列腺液常规无明显变化,差异无统计学意义(P0.05),NIH-CPSI评分、中医症状评分均有明显改善,差异均具有统计学意义(均P0.05),且治疗组明显优于对照组,差异具有统计学意义(P0.05)。结论清淋合剂治疗慢性前列腺炎(湿热下注型)临床疗效优于传统八正散组方,能更好地改善患者临床症状。     

通前络汤与前列倍喜胶囊治疗慢性前列腺炎的比较分析

目的:研究通前络汤在慢性前列腺炎患者中的临床疗效,为临床提供依据。方法:选取2014年1月至2016年5月医院诊治的慢性前列腺炎患者100例,根据治疗措施将患者分为对照组50例和观察组50例。对照组口服前列倍喜胶囊治疗,3次/d,2g/次;观察组通前络汤治疗,1剂/d,250mL/次,分早晚服用,比较两组临床疗效。结果:两组治疗前疼痛不适、排尿情况、症状影响、生活质量及总评分差异无统计学意义(P0.05);观察组治疗后疼痛不适、排尿情况、症状影响、生活质量及总评分,显著低于对照组(P0.05);两组患者治疗前炎症指标IL-8、TNF-α及IFN-γ水平差异无统计学意义(P0.05);观察组治疗后炎症指标IL-8、TNF-α及IFN-γ水平,显著低于对照组(P0.05)。结论:与前列倍喜胶囊治疗相比,慢性前列腺炎患者采用通前络汤治疗效果理想,能改善患者症状,减轻炎症指标,值得推广应用。     

复方青黛胶囊对银屑病微环境下HaCaT细胞分泌细胞因子的影响

目的探讨复方青黛胶囊在银屑病微环境下对HaCaT细胞IL-2、IL-6、IL-8、IL-18、IFN-γ表达水平的影响,初步阐明复方青黛胶囊治疗银屑病的可能机制。方法在银屑病微环境下观察复方青黛胶囊对HaCaT细胞IL-2、IL-6、IL-8、IL-18、IFN-γ表达水平的影响,并设空白对照和阿维A阳性对照。将HaCaT细胞分为4组,对照组(C组)不加任何干预;模型组(M组):采用M5(IL-1a、IL-17A、IL-22、抑癌蛋白M和TNF-α)模拟银屑病炎性微环境造模;复方青黛胶囊组(MF组)在模型组基础上加10%复方青黛胶囊培养72 h;阿维A组(MA组)在模型组基础上+10μmol/L阿维A培养72 h。采用ELISA方法检查各组细胞培养上清中IL-2、IL-6、IL-8、IL-18、IFN-γ的水平。结果M组与C组相比,IL-6、IL-8、IL-2、IFN-γ、IL-18水平显著升高,差异均有统计学意义(P均<0.05);MF组与M组相比IL-6、IL-8、IL-2、IFN-γ、IL-18水平显著降低,差异均有统计学意义(P均<0.05);MA组与M组相比IL-6、IL-2、IFN-γ、IL-18水平显著降低,差异均有统计学意义(P均<0.05),两组IL-8水平比较差异无统计学意义(P>0.05);MF组与MA组比较IL-6、IL-8、IFN-γ水平差异均有统计学意义(P均<0.05),两组IL-2、IL-18水平差异无统计学意义(P均>0.05)。结论复方青黛胶囊可能通过降低IL-6、IL-8、IL-2、IFN-γ、IL-18等银屑病相关炎性因子的水平而发挥治疗银屑病的作用。     

早期梅毒患者血清IL-27、IL-33水平检测

目的:研究早期梅毒患者血清IL-27、IL-33水平。方法:以我院2014年1月至2015年1月期间接诊的80例早期梅毒患者作为研究对象,根据其治疗效果将其分为梅毒血清转阴组和梅毒治疗无效组两组,每组各40人,然后再选择40例正常人群作为对照组。在治疗前,对三组患者的血清RPR滴度进行检测;在治疗前后,分别对三组患者的血清IL-27和血清IL-33水平进行检测,比较其在治疗前后的不同以及各组患者之间的差异,并对其结果进行分析。结果:治疗前,梅毒血清转阴组以及梅毒治疗无效组的血清RPR滴度均没有明显的差异(P0.05),具有可比性。治疗前,三组患者的血清IL-27水平具有明显的差异(P0.05);治疗后,梅毒血清转阴组患者以及正常对照组患者的血清IL-27水平与治疗前比较,没有明显的变化(P0.05),而梅毒治疗无效组患者的IL-27水平变化明显(P0.05);治疗前,梅毒血清转阴组和梅毒治疗无效组两组患者的血清IL-33水平没有明显的差异(P0.05),而两组患者的血清IL-33水平均明显高于正常对照组(P0.05);治疗后,梅毒治疗无效组患者以及正常对照组患者的血清IL-33水平与治疗前比较,没有明显的变化,差异无统计学意义(P0.05),而梅毒血清转阴组患者的IL-33水平变化明显。结论:在患者的血清中,IL-27及IL-33均参与患者机体对梅毒螺旋体的免疫应答,其中IL-27水平对于机体清除梅毒螺旋体具有更加重要的意义,对于早期的梅毒患者,在患者体内有较高水平的IL-27,则其血清RPR转阴可能性更大,具有临床应用价值,值得推广。     

温针灸关元穴配合前列欣胶囊治疗慢性非细菌性前列腺炎的疗效观察

目的:观察温针灸关元穴配合前列欣胶囊治疗慢性非细菌性前列腺炎(CAP)的临床疗效和慢性前列腺症状指数(NIH-CPSI)变化。方法:将60例CAP患者随机分为治疗组和对照组各30例,治疗组每日给予温针灸关元穴配合前列欣胶囊治疗,4粒/次,3次/d;对照组单纯给予前列欣胶囊。2周为1疗程,治疗2个疗程后观察两组疗效和治疗前后NIH-CPSI评分变化。结果:治疗组总有效率为93.3%,对照组为80%,两组间差异有统计学意义(P0.05);治疗组治疗前后NIH-CPSI评分,差异具有统计学意义(P0.01);治疗组治疗后NIH-CPSI评分与对照组比较,差异具有统计学意义(P0.01),两组治疗前后NIHCPSI差值比较也有统计学差异(P0.05)。结论:温针灸关元穴配合前列欣胶囊治疗CAP的临床疗效优于单纯药物组,能显著缓解患者症状。     

前列舒通胶囊合用喹诺酮类抗生素治疗慢性细菌性前列腺炎的临床研究

目的:观察前列舒通胶囊联合喹诺酮类抗生素治疗慢性细菌性前列腺炎的临床疗效及安全性,为慢性细菌性前列腺炎的治疗提供参考。方法:选择我院2015年2月至2016年1月门诊收治的80例慢性细菌性前列腺炎患者作为研究对象,根据所采用的治疗方法的不同,将其分为对照组和观察组,对照组44例采用喹诺酮类抗生素治疗,观察组36例采用前列舒通胶囊联合喹诺酮类抗生素治疗,两组患者均连续治疗8周。采用美国国立卫生院制定的《慢性前列腺症状指数评分》(NIH-CPSI)对两组患者治疗前、治疗4周、治疗8周的症状进行评价比比较,对两组患者治疗前后的前列腺按摩液进行白细胞计数检查并比较,两组患者治疗8周后采用NIH-CPSI与前列腺按摩液白细胞计数指标判断临床疗效并比较,统计两组患者治疗期间的不良反应并比较。结果:两组患者治疗前NIH-CPSI评分、前列腺按摩液白细胞计数比较差异均无统计学意义(P0.05);两组患者治疗4周、8周后NIH-CPSI指数与前列腺按摩液白细胞计数均较治疗前明显降低,且治疗后观察组NIH-CPSI与前列腺按摩液白细胞计数明显低于对照组(P0.05)。治疗8周后两组患者临床总有效率比较,观察组总有效率明显高于对照组(P0.05)。两组患者治疗期间未收集到明显的不良反应。结论:前列舒通胶囊辅助喹诺酮类抗生素治疗慢性细菌性前列腺炎临床疗效确切,安全性高,具有较高的临床价值。     

尿道炎后慢性前列腺炎患者精浆中TNF-α及IL-18的检测及临床意义

目的检测尿道炎后慢性前列腺炎患者精浆中肿瘤坏死因子(TNF-α)、白细胞介素18(IL-18)的浓度并探讨其临床意义。方法采用ELISA法测定精浆中IL-18浓度;放射免疫法测定精浆中TNF-α浓度。结果尿道炎后慢性前列腺炎(Ⅱ型、Ⅲ型)患者精浆中TNF-α浓度与对照组比较,差异均有显著性(P<0.01);而在慢性细菌性前列腺炎(Ⅱ型)和慢性非细菌性前列腺炎(Ⅲ型)之间比较,差异无显著性(P>0.05)。尿道炎后慢性前列腺炎(Ⅱ型、Ⅲ型)患者精浆中IL-18浓度与对照组、Ⅱ型和Ⅲ型比较,差异均有显著性(P<0.01)。结论尿道炎后慢性前列腺炎患者精浆中TNF-α、IL-18明显升高,提示TNF-α,IL-18在尿道炎后慢性前列腺炎的发病过程中起了重要的作用。     

生殖支原体与性病性尿道炎后慢性非细菌性前列腺炎的关系

目的探讨生殖支原体(Mg)在性病性尿道炎后慢性非细菌性前列腺炎发病中的意义。方法同时收集78例性病性尿道炎后慢性非细菌性前列腺炎患者(炎症亚组45例和非炎症亚组33例)与51例无前列腺炎者的首段尿沉渣和前列腺液标本,均采用PCR方法检测Mg-DNA。结果所有尿沉渣标本Mg检测阴性。性病性尿道炎后慢性非细菌性前列腺炎组前列腺液Mg检出率为6.4%(5/78),无前列腺炎组为0,但差异无统计学意义(P=0.13);炎症亚组(11.1%,5/45)与非炎症亚组(0)也无统计学差异(P=0.14)。结论Mg可从前列腺液中检出,但尚不足提示其与性病性尿道炎后慢性非细菌性前列腺炎有关。     

白介素-19、20和24与银屑病的关系

白介素-19、20和24是新近发现的3种细胞因子，生物功能尚不完全明了，结构上与白介素-10有很高的同源性。相应的受体和基因结构也明显关联，它们及其受体在结构上有同源性，功能上有联系，但又有各自的独特陛。初步研究显示，它们主要分布在银屑病皮损的表皮，在银屑病炎症中发挥重要作用。     

IL-12 and IL-23 affect photocarcinogenesis differently

Induction of DNA damage by UVR is the key event in photocarcinogenesis. IL-12 and IL-23 are related heterodimeric cytokines consisting of a common p40 unit and a p35/IL-12 and a p19/IL-23 chain, respectively. Both exert immunomodulatory activities but are also found to reduce UVR-induced DNA damage presumably via induction of DNA repair. As both cytokines are also produced in the skin, they may mitigate the risk to develop UVR-induced skin cancer. This appears to be the case as mice lacking p40 were previously shown to be at higher risk for skin tumors upon chronic UVR exposure. As these mice express neither IL-12 nor IL-23, the individual effects of IL-12 or IL-23 could not be evaluated. Thus, mice lacking p35 (IL-12p35-/-) or p19 (IL-23p19-/-) were subjected to chronic UVR exposure. The Kaplan-Meier analysis indicated a significantly increased probability of tumor development in IL-23p19-/- but not in IL-12p35-/- mice. Taken together, in our model, loss of IL-23, but not of IL-12, enhances development of UVR-induced skin tumors, indicating that IL-23 but not IL-12 may counteract photocarcinogenesis. This may have impact on the development of future strategies utilizing antibodies against IL-12 and IL-23, respectively, for the treatment of inflammatory dermatoses.     

IL-17和IL-23拮抗剂治疗银屑病的研究进展

目前美国食品药品监督局（FDA）批准用于临床的IL-17抗体拮抗剂包括secukinumab、ixekizumab和brodalumab,三者治疗中度至重度斑块状银屑病皮损清除均优于对照组TNF-α拮抗剂,且安全性及耐受性均较好。三种IL-23拮抗剂risankizumab,guselkumab和tildrakizumab目前均在III期临床试验中,初步临床试验结果表明三种药物治疗银屑病较对照组TNF-α拮抗剂具有更快达到皮疹改善、更高皮疹清除率和注射次数更少的优势。本文就上述六种生物制剂治疗银屑病的研究进展进行了综述。     

Role of IL-17 and IL-22 in autoimmunity and cancer

The dysregulation of inflammatory cytokines can cause a variety of diseases, such as autoimmunity and cancer. Since their identification in 2005, Th17 cells and its signature cytokine IL-17, have been implicated in the pathogenesis of autoimmune diseases such as psoriasis and rheumatoid arthritis (RA), and inflammatory associated cancers such as colorectal carcinoma (CRC). Recently, IL-22 a Th17 related cytokine has been shown to be pathogenic in psoriasis and RA. In this review, we will summarize the biological functions of IL-17 and IL-22, their role in autoimmune diseases and briefly review results from clinical trials targeting IL-17 or its receptor for the treatment of autoimmune diseases. Next, we will discuss pre-clinical and clinical data supporting the rationale of targeting other cytokines implicated in the Th17/IL-17 pathway, such as IL-22 and IL-23. Finally, we discuss the role of IL-17, and in particularly IL-22 in tumour immunity and possible therapeutic interventions.     

产妇宫内感染对早产儿IL-1β、IL-6、IL-8、IL-10和TNF-α影响情况研究

目的:探讨产妇宫内感染对早产儿IL-1β、IL-6、IL-8、IL-10和TNF-α的影响。方法:回顾性分析2015年8月至2016年10月在我院接受分娩的宫内感染产妇的早产儿的临床资料,同时选取同期在我院分娩的非宫内感染产妇的早产儿作为对照。观察两组早产儿血清炎症因子水平、NIHSS评分、肾功能、心肌酶指标和出生时一般情况的差异。结果:宫内感染组早产儿的IL-1β、IL-6、IL-8、IL-10和TNF-α水平均高于对照组(t=7.044、12.208、7.289、20.185、5.421,P0.001);宫内感染组早产儿的Sp O2、Pa O2低于对照组(t=-6.353、-35.142,P0.001),Pa CO2水平高于对照组(t=35.720,P0.001);宫内感染组早产儿的NIHSS评分高于对照组(t=50.424,P0.001),出生1min和5min的Apgar评分低于对照组(t=-3.475、-4.398,P0.001);宫内感染组早产儿的尿素氮、肌酐和CK-MB水平均高于对照组(t=49.014、11.611、16.458,P0.001);Pearson相关分析法结果显示,宫内感染早产儿血清IL-1β、IL-6、IL-8、IL-10和TNF-α水平与NIHSS评分、肾功能、心肌酶指标水平正相关,与Apgar评分负相关。结论:宫内感染产妇分娩的早产儿的血IL-1β、IL-6、IL-8、IL-10和TNF-α水平较高,且与患儿的NIHSS评分、肾功能、心肌酶指标密切相关。     

Histamine-induced IL-6 and IL-8 production are differentially modulated by IFN-gamma and IL-4 in human keratinocytes

It is known that large amounts of histamine are stored in mast cells located in the superficial dermis of the skin and can be released upon appropriate stimulation. However, the effects of histamine on keratinocyte function have not been well characterized. We therefore examined the capacity of histamine to modulate the production of interleukin (IL)-6 and IL-8 by keratinocytes. We found that histamine significantly augmented the production of IL-6 and IL-8 in a dose- and time-dependent manner. The enhancing effects of histamine were completely inhibited by a potent H1 receptor (H1R) antagonist, emedastine difumarate. Pyrilamine (a much weaker H1R antagonist) and cimetidine (an H2R antagonist) only partially inhibited the enhancing effects of histamine. The histamine-induced up-regulation of IL-6 and IL-8 production, however, was completely abrogated by a combination of pyrilamine and cimetidine. The IL-6 production was significantly enhanced by interferon (IFN)-gamma. Interestingly, IFN-gamma and IL-4 both significantly augmented the histamine-induced IL-6 production. On the other hand, the production of IL-8 was inhibited by IFN-gamma, and IFN-gamma and IL-4 both completely abrogated the histamine-induced IL-8 production. These results suggest that the histamine-induced IL-6 production and IL-8 production are differentially regulated by IFN-gamma and IL-4. Histamine may be an important modulator of cytokine production in epidermal milieu.     

Role of fibroblasts in the regulation of proinflammatory interleukin IL-1, IL-6 and IL-8 levels induced by keratinocyte-derived IL-1

Received: 15 May 1995     

白介素-1和银屑病

银屑病的发病机制至今尚未完全明确，银屑病皮损中存在细胞因子的异常表达，IL—1是重要的炎症递质，在银屑病皮损中IL—1表达异常。研究表明，IL—1在银屑病发病中起重要作用。     

IL-17 and infections

IL-17 immunity has been shown to be essential for mucocutaneous protection against Candida albicans in mice and humans. However, mice with defective IL-17 immunity display broader susceptibility, as they are also prone to infections with diverse infectious agents at various sites. Humans with genetic defects affecting their IL-17 immunity usually suffer from chronic mucocutaneous candidiasis (CMC): recurrent or persistent infections of the skin, nails, and mucosae with C. albicans, with or without other clinical signs. Most patients with autosomal dominant (AD) hyper-IgE syndrome (HIES) due to STAT3 deficiency or AD STAT1 gain-of-function display impaired IL-17-producing T-cell development, and CMC is one of their principal clinical manifestations. Similarly, patients with autosomal recessive (AR) autoimmune polyendocrine syndrome type 1 (APS-1) caused by AIRE deficiency have high levels of neutralizing autoantibodies against IL-17A, IL-17F and/or IL-22 and present CMC as their only infectious disease. Finally, CMC is the main clinical phenotype observed in patients with inborn errors specifically affecting IL-17 immunity. Indeed, patients with AD IL-17F deficiency or AR IL-17RA or ACT1 deficiency display CMC and, to a lesser extent, superficial staphylococcal diseases. Candida infection was recently reported in psoriasis patients treated with anti-IL-17A antibodies. Careful monitoring for CMC is thus important during anti-IL-17 treatment.