系统性红斑狼疮患者血清IL-8、IL-10、IFN-γ的变化及意义

系统性红斑狼疮 (SL E)是一种以多脏器受累及血清中含有多种自身抗体为特征的自身免疫性疾病。实验证明 ,白介素- 8(IL- 8)、白介素 - 6 (IL- 6 )、白介素 - 10 (IL- 10 )在 SL E的发生发展中起重要作用。2 0 0 2年 3月至 2 0 0 3年 2月 ,我们对 48例SL E患者和 45例健康对照者进行了血清 IL - 8、IL - 10及 IFN-γ测定。现报告如下。临床资料 :观察组 48例 ,均为我院的 SL E患者。男 6例 ,女 42例 ;年龄 13～ 5 0岁 ,平均 (35 .8± 12 .4)岁 ;均符合美国风湿病协会 1982年诊断标准。对照组 45例 ,为同期在我院健康查体者 ,男 5例…     

自然流产与弓形虫感染的关系

弓形虫病是全球性人畜共患疾病 ,其主要感染途径为接触感染弓形虫的猫、狗或其它动物以及食用污染的生肉、生乳等。有报道孕妇弓形虫感染率约 10 % ,且新近感染和活动性感染可占 4 5 % [1 ,2 ] ,已成为影响胎儿、新生儿正常生长发育的重要原因。孕妇弓形虫感染有逐年上升趋势 ,我国每年大约有 9万例先天性弓形虫患儿出生 [3]。为探讨弓形虫与自然流产的关系 ,我们应用 PCR技术对自然流产的患者血清进行弓形虫 (Tox) - DNA检测 ,现报道如下。1　资料与方法1.1　研究对象　 1997年 1月～ 1998年 9月在我院就诊的自然流产者 12 8例 ,为观察…     

IL-10、IL-12与吉兰-巴雷综合征相关性研究

目的　探讨白细胞介素 10 (IL 10 )、白细胞介素 12 (IL 12 )与吉兰 巴雷综合征 (GBS)的关系。方法　用免疫酶联双抗体夹心 (ELISA)法对 3 6例急性期、恢复期GBS患者血清及脑脊液 (CSF)中IL 10、IL 12进行检测。结果　GBS急性期患者血清IL 10水平较对照组未见显著变化 (P >0 0 5 ) ,恢复期则明显升高 (P <0 0 1) ;GBS急性期患者血清IL 12水平较对照组明显升高 (P <0 0 1) ,且其水平与病情分级正相关 ,恢复期明显下降 ;恢复期血清IL 10与IL 12存在负直线相关关系 ;GBS急性期、恢复期CSF中IL 10、IL 12的水平与血清中类似。结论　IL 10、IL 12与GBS的致病过程明显相关 ,IL 10通过下调免疫起保护作用 ,它可能使脱失髓鞘修复 ;IL 12上调免疫参与致病 ,它可能促使脱髓鞘和轴索变性 ;GBS患者血 神经、脑屏障受到一定程度破坏     

病毒性肝炎患者血清IL-12及IL-18水平变化的研究

为探讨病毒性肝炎和肝炎后肝硬化患者血清中IL－12、IL－18的变化规律及其临床意义，应用ELISA法测定血清中IL－12及IL－18含量。各组肝炎及肝硬化患者IL－12及IL－18水平明显高于对照组（P＜0．001），以重型肝炎水平最高。重型肝炎死亡病例IL－12及IL－18明显高于生存组（P＜0．01）。IL－12和IL－18与AST、ALT及TBil正相关，且IL－12与CⅣ及HA水平正相关。IL－12、IL－18可能参与了病毒性肝炎和肝炎后肝硬化患者的肝脏炎性损伤过程，重型肝炎患者联合检测血清IL－12及IL－18有助于判断其预后。     

乙酰螺旋霉素联合阿奇霉素治疗孕期弓形虫感染的临床效果

目的 目的 探讨螺旋霉素联合阿奇霉素治疗孕期弓形虫感染的综合治疗方法。方法 方法 应用ELISA方法检测孕妇血清 特异性弓形虫IgG和IgM抗体, 并采用PCR方法检测羊水中弓形虫感染情况; 选取其中3例弓形虫IgM抗体阳性孕妇作为 治疗对象, 应用螺旋霉素联合阿奇霉素进行综合治疗, 通过血清学特异性抗体检测和PCR检测结果初步判断其疗效。结 结 果 果 经ELISA检测, 孕期妇女血清特异性弓形虫IgG和IgM抗体的阳性率分别为5.97% （17/285） 和1.05% （3/285）, 对3例血 清IgM抗体阳性孕妇的羊水进行PCR检测特异性弓形虫基因, 有2例呈阳性。经过2种药物的联合治疗, 这2例孕妇血清 特异性弓形虫IgM抗体滴度阴转, PCR检测产妇脐带血中弓形虫基因为阴性。结论 结论 螺旋霉素联合阿奇霉素治疗孕期弓 形虫感染安全、 有效。     

老年糖尿病肾病血IL-1、IL-6水平测定及临床意义

研究发现 ,糖尿病肾病与白介素 - 1(IL- 1)、白介素 - 6 (IL- 6 )密切有关 〔1〕。我们测定了老年及非老年糖尿病肾病血 IL - 1、IL -6水平 ,探讨其临床意义。1　对象和方法1.1　 对象　选择老年 2型糖尿病 39例 ,年龄 6 0～ 72岁 ,病程0 .5～ 2 5年 ,其中糖尿病肾病 19例 (老年 DN组 ) ,其余 2 0例为老年糖尿病组 (老年 DM组 ) ,合并糖尿病眼底病变者 17例 ;非老年 2型糖尿病 38例 ,年龄 35～ 5 8岁 ,病程 1个月～ 10年 ,其中糖尿病肾病 18例 (非老年 DN组 ) ,其余 2 0例为非老年糖尿病组 (非老年 DM组 ) ,合并眼底病变者 12例。均…     

河北隆化地区异常妊娠结局孕妇弓形虫感染血清学调查

目的了解河北省隆化地区异常妊娠结局孕妇弓形虫感染状况。方法选择2013-1-2016-6隆化县妇幼保健院就诊的异常妊娠结局孕妇393例为调查对象,根据检测结果分为急性感染组、既往感染组和活动性感染组,正常对照组为无异常妊娠史的孕妇256例。采用酶联免疫吸附实验检测各组孕妇血清弓形虫IgM、IgG抗体。采用问卷调查方式对各组孕妇进行弓形虫感染危险因素调查。结果异常妊娠结局孕妇弓形虫感染率为27.23%(107/393),显著高于正常对照组的8.20%(21/256),差异有统计学意义(χ~2=35.46,P0.01)。异常妊娠结局孕妇急性感染率为6.87%(27/393)、既往感染率为18.58%(73/393)、活动性感染率为2.54%(10/393),分别高于正常对照组的1.17%(3/256)、7.03%(18/256)和0(0/256),差异均有统计学意义(χ~2=11.43、17.15、7.90,P均0.01)。感染组中从事接触生肉的职业、饲养猫狗宠物、品尝生肉馅、菜板生熟不分、常吃涮锅或烧烤、经常在外就餐的比例显著高于非感染组,差异均有统计学意义(χ2=12.08、29.23、8.55、13.41、7.28、6.06,P均0.05)。结论本地区异常妊娠结局孕妇弓形虫感染率较高,避免与宠物密切接触、不食用未煮熟的肉类以及加强个人卫生防护等是避免弓形虫感染的重要环节。     

弓形虫特异IgE的诊断价值

对弓形虫感染的诊断和分期,多根据IgA,IgM和IgG 3种免疫球蛋白的检测结果,而对IgE诊断意义的研究则少见。作者报告用多克隆抗人IgE血清进行改良的免疫吸附凝集试验(ISAGA)检测孕妇血清中弓形虫特异IgE抗体的结果。 检测的孕妇血清共2279份,其中1991份为未经选择的血清,其余288份为经选择的各期弓形虫感染的人血清。1991份血清先用以下试验进行筛选:弓形虫染色试验、直接凝集试验、IgG-ELISA、IgM-ELISA或直     

妊娠合并弓形虫感染对胎婴儿的影响

弓形虫是一种专性细胞内寄生的原虫 ,感染人和牲畜 ,引起人和牲畜弓形虫病 ,孕妇感染了弓形虫除了自身患病外 ,还能通过胎盘将弓形虫垂直传播给胎儿 ,引起流产 ,死胎、胎儿畸形。现将我院 10 2例孕妇流产、死胎、胎儿畸形中 ,共检出 12例弓形虫感染患者 ,分析如下。1　材料与方法1 1　一般资料　选择 1999年 1月～ 2 0 0 0年 12月 ,妊娠合并晚期流产 4 5例、死胎 4 0例、胎儿畸形 17例 ,共 10 2例孕妇行血清弓形虫检测。检出阳性 12例占 11 76%。其中干部 4 7例 ,占 4 6 0 7% ;工人2 6例、占 2 5 4 9% ;个体及经商 2 9例 ,占 2 8 4 3%。其中…     

急性冠状动脉综合征患者血清白介素-18水平及其临床意义

目的　评价血清白介素 18(IL 18)水平与急性冠状动脉综合征的关系及其临床意义。方法　 2 0 0 3年 6月至2 0 0 4年 1月入选本院心脏科门诊和住院患者 88例 ,其中冠心病患者 70例 ,非冠心病者 18例 ,分为急性心肌梗死(AMI)组、不稳定型心绞痛 (UAP)组、稳定型心绞痛 (SAP)组及对照组。采用ELISA法测定IL 18,放射免疫平衡竞争法检测白介素 6 (IL 6 )。应用SPSS11.0进行统计分析 ,P <0 .0 5为具有统计学差异。结果　血清IL 18,6在AMI组和UAP组显著高于SAP组和对照组 (P <0 .0 1) ;血清IL 6水平在AMI组、UAP组和SAP组之间均具有显著差异 (P<0 .0 1) ;相关性分析显示IL 18,6与射血分数 (EF)均呈显著负相关 (IL 6 ,r=- 0 .70 7,P <0 .0 1;IL 18,r =- 0 .717,P <0 .0 1) ,IL 18与IL 6呈显著正相关 (r=- 0 .92 0 ,P <0 .0 1)。结论　IL 18可以反映动脉粥样硬化斑块的严重程度和稳定性状态 ,可作为监测病情的临床生化指标 ,推测其与IL 6、EF可同作为冠心病心血管事件的预测因子。     

DOWN-REGULATION OF FIBRINOGEN BIOSYNTHESIS BY IL-4, IL-10 AND IL-13

High levels of fibrinogen are recognized as an important vascular risk factor; however, it is not known if the increase of plasma fibrinogen is directly responsible for this risk, or is only a marker of vascular inflammation. To support this second hypothesis, Oncostatin M (OSM) is a potent stimulator of fibrinogen biosynthesis and induces smooth muscle cell proliferation. In the same way, we analysed whether interleukin-4 (IL-4), interleukin-10 (IL-10) or interleukin-13 (IL-13), which protect vessel walls from monocytes injuries leading to atherosclerosis, could influence fibrinogen biosynthesis. The two levels of regulation of fibrinogen biosynthesis were tested: firstly, the direct effect of these cytokines on fibrinogen production by the hepatoma cell line Hep G2, and secondly their effect on the secretion of hepatocyte stimulating factor (HSF) activity in the supernatant of lipopolysaccharide (LPS)-activated monocytes. IL-4 and IL-13 added to Hep G2 cells down-regulated both the increase of fibrinogen secretion induced by IL-6 and fibrinogen mRNA levels, this effect being more pronounced when Hep G2 were preincubated with the two cytokines before IL-6 addition. The effect of IL-10 was evidenced only on mRNA expression. IL-10 and IL-13 dose-dependently decrease HSF activity secreted by LPS-activated monocytes, whereas IL-4 had no effect. However, the three cytokines decreased HSF activity when monocytes were incubated with the cytokines before LPS activation. The effects of these cytokines on HSF activity are related to variations of IL-6 and OSM secretion. Our data strengthen the hypothesis that the fibrinogen level is a marker of vascular disease, since cytokines which have a protective vascular effect down-regulate fibrinogen production.     

Serum IL-4, IL-10 and IL-6 levels in inflammatory arthritis

As the available in vitro and in vivo data suggest that interleukin (IL)-4 and IL-10 have immunosuppressive activity, our hypothesis was that serum IL-4 and IL-10 levels would correlate inversely with parameters of inflammation in patients with inflammatory arthritis. IL-4 was detected in the serum of 12 out of 140 patients with rheumatoid arthritis (RA), which was increased compared to the proportion found with patients with osteoarthritis (OA; P< 0.02). In addition, IL-4 was detected in the serum of 2 of 19 patients with systemic lupus erythematosus (SLE), 2 of 24 patients with psoriatic arthritis and 1 of 5 patients with Behçet's syndrome. No IL-4 was detected in patients with the following conditions: OA (58 patients), gout (17 patients), ankylosing spondylitis (6 patients), Reiter's syndrome (6 patients), polymyalgia rheumatica (6 patients), temporal arteritis (5 patients) and scleroderma (3 patients). No IL-10 was detected in any of the sera tested. We discuss the possible relevance of these results to the regulation of the immune response evident in inflammatory arthritis.     

白细胞介素10基因转染对小鼠心脏移植排斥反应中细胞因子表达的影响

目的 :探讨白细胞介素 10 (IL 10 )基因转染对小鼠心脏移植排斥反应中IL 12、IL 15、IL 18和IL 4表达的影响。方法 :采用小鼠颈部心脏移植模型 ,随机分为 3组 :对照组、移植组和IL 10组。于术后第 5天取移植心脏 ,用逆转录聚合酶链式反应 (RT PCR)法观察IL 12、IL 15、IL 18、IL 4及IL 10的表达情况。结果 :移植组IL 12、IL 15、IL 18表达与对照组比较明显升高 ,IL 10、IL 4表达显著降低 (均P <0 .0 1)。IL 10组IL 12、IL 15、IL 18表达与移植组比较明显降低 ,而IL 4及IL 10表达显著升高 (均P <0 .0 1)。结论 :IL 10基因转染抑制心脏移植排斥反应主要与其抑制IL 12、IL 15、IL 18等Th1型细胞因子的表达 ,促进Th2型细胞因子IL 4的表达 ,使免疫反应由Th1型向Th2型偏移有关     

Expression of IL-23 and IL-17 and effect of IL-23 on IL-17 production in ankylosing spondylitis

The objective of this study was to investigate the expression of IL-23 and IL-17 and the influence of IL-23 on IL-17 production in ankylosing spondylitis (AS) patients. IL-23 and IL-17 levels in the serum and supernatants of cultured peripheral blood mononuclear cells (PBMCs) were determined by ELISA. IL-23p19 mRNA expression in PBMCs were analyzed using RT-PCR. The patients with AS at active stage showed elevated levels of IL-23 and IL-17 in the serum and supernatants of cultured PBMCs. A higher expression of IL-23p19 mRNA in PBMCs of AS patients was also observed. A significantly enhanced production of IL-17 in the supernatants of cultured PBMCs was found in the presence of recombinant IL-23 and this effect was more significant in patients with AS. The results suggest that IL-23 and IL-17 may play critical roles in the pathogenesis of AS and IL-23-stimulated production of IL-17 by PBMCs may be responsible for the development of AS.     

IL-12及IL-10在桥本甲状腺炎发病机制中作用的研究进展

桥本甲状腺炎是一种常见的慢性自身免疫性疾病,表现为辅助性T细胞(Th)1占优势。 Th1类细胞因子白细胞介素(IL)-12表达增加,在该病的诱发及慢性迁延中起重要作用,Th2类细胞因子IL-10表达的下调也与该病有关,且随病情变化二者表达水平有所不同。因此,这些细胞因子可作为病情变化的监测指标,并且可通过调节细胞因子的表达水平从而使失衡的Th1／Th2细胞趋于平衡。这可能为桥本甲状腺炎的治疗开拓一条新途径。     

A complex of the IL-1 homologue IL-1F7b and IL-18-binding protein reduces IL-18 activity

IL-1F7 was discovered in expressed sequence tag databases as a member of the increasing family of proteins sharing sequence homology to IL-1alpha/beta, IL-1Ra, and IL-18. In the present study using immunohistochemical staining, IL-1F7 was localized in human peripheral monocytic cells, suggesting its role in immune regulation. Recombinant human IL-1F7b was shown to bind to the IL-18Ralpha but without IL-18 agonistic or antagonistic function. Using chemical cross-linking, we observed that, unlike IL-18, IL-1F7b fails to recruit the IL-18Rbeta chain to form a functionally active, ternary complex with the IL-18Ralpha chain. IL-1F7b shares two conserved amino acids with IL-18 (Glu-35 and Lys-124), which participate in the interaction of IL-18 with the IL-18Ralpha chain as well as the IL-18-binding protein (IL-18BP), a secreted protein that neutralizes IL-18 activity. In testing whether IL-1F7b interacts with IL-18BP, we unexpectedly observed that IL-1F7b enhanced the ability of IL-18BP to inhibit IL-18-induced IFNgamma by 25-30% in a human natural killer cell line. This effect was observed primarily at limiting concentrations of IL-18BP (3.12-12.5 ng/ml) and at a 50- to 100-fold molar excess of IL-1F7b. Similar results were obtained by using isolated human peripheral blood mononuclear cells. To study the molecular basis of this effect we performed binding studies of IL-1F7b and IL-18BP. After cross-linking, a high molecular weight complex consisting of IL-1F7b and IL-18BP was observed on SDS/PAGE. We propose that after binding to IL-18BP, IL-1F7b forms a complex with IL-18Rbeta, depriving the beta-chain of forming a functional receptor complex with IL-18Ralpha and thus inhibiting IL-18 activity.     

Prognostic values of IL-6, IL-8, and IL-10 in acute pancreatitis

GOALS: The prognostic importance of interleukin-6 (IL-6), IL-8, and IL-10 in the prediction of acute pancreatitis severity. BACKGROUND: Early assessment of severity in acute pancreatitis could help the patients who are at risk of developing complications. Unfortunately, the used prognostic scoring systems generally are only moderately accurate in assessing disease severity. STUDY: We studied 117 consecutive patients with a diagnosis of acute pancreatitis admitted to our hospital during the past 2 years. Laboratory parameters and cytokines were analyzed from serum taken routinely on admission. Severity criteria were noted for each patient using Ranson, Glasgow, and APACHE II scoring systems. Local and systemic complications, developed during a follow-up period, were classified by Atlanta criteria. RESULTS: IL-6 was the only parameter that statistically significantly predicted complicated acute pancreatitis (P<0.05). IL-8 and IL-10 and the 3 prognostic scoring systems used did not properly assess complicated versus noncomplicated acute pancreatitis. CONCLUSIONS: Our prospective study supported the potential importance of IL-6 in the early assessment of complicated acute pancreatitis, but also suggested that pancreatitis classified as complicated in a large number of patients could not be correctly predicted with the Ranson, Glasgow, and APACHE II scoring systems.     

低氧条件下 IL-6、IL-8、IL-10作用机制研究进展

低氧(hypoxia)是机体循环血液中氧分压较低的状态,缺氧则引发机体的一系列反应。随着研究的深入,发现机体内不但存在氧化应激反应,还伴随着一系列炎症反应,IL-6、IL-8、IL-10作为常见的炎症因子也成为研究热点,本文就低氧条件下三种 IL 的作用机制作一总结,为低氧造成的机体损伤提供更多理论依据,为低氧损伤的预防及治疗提供新靶点。     

Whole Tumor Cell Vaccine Adjuvants: Comparing IL-12 to IL-2 and IL-15

Cancer immunotherapy (passive or active) involves treatments which promote the ability of the immune system to fight tumor cells. Several types of immunotherapeutic agents, such as monoclonal antibodies, immune checkpoint inhibitors, non-specific immunomodulatory agents, and cancer vaccines are currently under intensive investigation in preclinical and clinical trials. Cancer vaccines induce permanent activation of the immune system and may be considered the most promising method for cancer treatment, especially in combination with other agents of passive immunotherapy. Among various approaches to cancer vaccines, whole tumor cell vaccines have been attracting attention for several years. Despite their low to moderate clinical effects, these vaccines have numerous advantages. Their ability to generate immune responses against tumor-associated antigens reduces the possibility for tumor cells to escape and facilitates the development of “off-the-shelf” allogeneic tumor vaccines. Understanding the reciprocal interactions between tumor cells and leukocytes is a key to harness the full potential of whole cell vaccination. Cytokines are considered as potent immunomodulatory molecules which behave as adjuvants in whole tumor cell vaccines. Improved mechanistic understanding of key cytokines in tumor immunity will serve as a resource for rational design of whole cell cancer vaccines. Although there are several reports about the use of different immunostimulatory cytokines as adjuvants, interleukin (IL)-12 appears to have superior effects compared to other cytokines. This review describes the effects of IL-12 compared to other immunomodulatory cytokines, such as IL-2 and IL-15, and highlights its application in whole cell tumor vaccination.