Mnk2和eIF4E在食管鳞状细胞癌中的表达及其临床意义

目的:探讨MAPK相互作用激酶-2(MAPK-interacting kinase-2,Mnk2)和真核细胞翻译起始因子4E(eukaryotic initiation factor 4E,eIF4E)在食管鳞状细胞癌中的表达及其与食管鳞状细胞癌临床病理特征的关系。方法:收集临床食管鳞癌石蜡标本98例及正常食管黏膜上皮组织20例,应用免疫组化SP法检测癌组织及正常食管黏膜组织中Mnk2和eIF4E的表达,并分析其与食管鳞癌临床病理特征的关系。结果:Mnk2在食管癌组织中的阳性率68.4%(67/98),eIF4E的阳性率为61.2%(60/98),Mnk2与eIF4E表达呈正相关(P0.05),且Mnk2蛋白过表达与食管鳞癌的浸润深度、病理分期密切相关(P0.05)。结论:Mnk2在食管癌组织中的过表达与浸润深度、TNM分期有关,同时与eIF4E在食管癌的表达相关,两者在食管癌的发展中有协同作用。     

食管鳞状细胞癌肿瘤间质成熟度的临床病理学意义及与PD-L1表达的相关性

目的 探讨食管鳞状细胞癌(esophageal squamous cell cancer,ESCC)中肿瘤间质成熟度(tumor stromal maturity,TSM)的临床病理学意义,及其作为免疫治疗疗效预测组织学特征的潜在价值.方法 收集129例ESCC手术切除标本,根据HE染色评估肿瘤间质并将其分为成熟型、中...     

PKM2与头颈部鳞状细胞癌淋巴结转移及临床分期相关性的Meta分析

目的探讨PKM2与头颈部鳞状细胞癌(head and neck squamous cell carcinoma, HNSCC)淋巴结转移及临床分期的相关性。方法检索PubMed和EMBASE数据库,最终纳入5篇文献共611例HNSCC组织标本进行Meta分析,分析指标包括淋巴结转移和临床分期。结果 HNSCC组织中PKM2表达与淋巴结转移(OR=0.43,95%CI=0.29～0.64,P0.000 1)和临床分期(OR=0.33,95%CI=0.22～0.48,P0.000 01)呈显著性相关。结论 HNSCC组织中PKM2表达与淋巴结转移及临床分期具有相关性,提示PKM2可作为HNSCC淋巴结转移及临床分期的预测指标。     

细胞角蛋白20的表达与乳腺癌进展、转移及预后的相关性

目的: 探讨细胞角蛋白20(CK20)在乳腺癌组织中的表达及其与乳腺癌进展、转移和预后的相关性.方法: 选取乳腺癌患者86例,以20例乳腺良性肿瘤组织作为对照,应用免疫组织化学染色方法检测CK20的表达,并分析其与乳腺癌临床病理特征及预后的关系.结果: CK20在乳腺癌组织中的阳性表达率为80.23%(69/86),明显高于在乳腺良性肿瘤中的表达[20.00%(4/20),P<0.01].CK20的表达与乳腺癌组织学分级(P<0.05) 和病理类型(P<0.01) 有关,与TNM 分期(r=0.86,P<0.05)、淋巴结转移(r=0.73,P<0.05) 和HER-2 (r=0.69,P<0.05) 呈正相关,与ER (r=-0.58,P<0.05) 呈负相关.CK20阳性表达组和CK20阴性表达组的5年生存率分别为38.46%(25/65)和66.67%(10/15),差异有统计学意义(P<0.01).结论: CK20与乳腺癌的进展、转移有关,作为新的乳腺癌标记物,有一定的临床实际应用价值.     

δ-连环蛋白在食管鳞状细胞癌中的表达及其与患者预后之间的关系

δ-连环蛋白(δ-catenin)作为连环蛋白家族中的一员,其在肿瘤组织中的表达及其临床意义仍不明确。本组旨在探讨δ-catenin在食管鳞状细胞癌(ESCC)中的表达及其预后价值。作者对299例ESCCδ-catenin免疫组化结果进行了评估。结果发现,47例ESCC邻近正常鳞状上皮δ-catenin均为     

P53和Id2蛋白在食管鳞状细胞癌的表达及其意义

目的研究P53、Id2蛋白在食管鳞状细胞癌组织的表达及其意义。方法采用免疫组织化学SP法和图像分析技术检测122例食管鳞状细胞癌及90例癌旁正常组织中P53、Id2的表达情况。结果 122例食管鳞状细胞癌中P53、Id2表达水平均高于癌旁正常组织(p0.01)。P53表达强度与患者的性别、年龄及肿瘤的分化程度未见明显相关性(p0.05),但与肿瘤的浸润深度及淋巴结转移情况相关(p0.05);Id2的表达与患者的性别、年龄及淋巴结转移情况未见明显相关性(p0.05),但与肿瘤的分化程度成负相关(p0.05),且与肿瘤浸润深度正相关(p0.05)。结论 P53、Id2在食管鳞状细胞癌的高表达可能作为判断食管鳞状细胞癌生物学行为的潜在指标。     

拓扑异构酶Ⅱα(TOP2A)基因的表达与CD4+T细胞在肝细胞癌中的数量相关性及临床预后意义

目的 分析拓扑异构酶Ⅱα(TOP2A)基因的表达与CD4+T细胞在肝细胞癌(HCC)中的数量相关性及临床预后意义.方法 利用基因注释资源数据库(BioGPS)、基因表达谱交互作用分析(GEPIA)和Kaplan-Meier Plotter数据库分析TOP2A mRNA在正常肝组织和HCC组织中的表达以及对HCC患者生存...     

P53和1d2蛋白在食管鳞状细胞癌的表达及其意义

目的研究P53、Id2蛋白在食管鳞状细胞癌组织的表达及其意义。方法采用免疫组织化学SP法和图像分析技术检测122例食管鳞状细胞癌及90例癌旁正常组织中P53、Id2的表达情况。结果122例食管鳞状细胞癌中P53、Id2表达水平均高于癌旁正常组织（P〈0．01）。P53表达强度与患者的性别、年龄及肿瘤的分化程度未见明显相关性（P〉0．05）,但与肿瘤的浸润深度及淋巴结转移情况相关（P〈0．05）;Id2的表达与患者的性别、年龄及淋巴结转移情况未见明显相关性（P〉0．05）,但与肿瘤的分化程度成负相关（P〈0．05）,且与肿瘤浸润深度正相关（P〈0．05）。结论P53、Id2在食管鳞状细胞癌的高表达可能作为判断食管鳞状细胞癌生物学行为的潜在指标。     

食管鳞状细胞癌组织中miR-21与ERK1/2表达及临床意义的研究

目的：研究miR-21和ERK1/2在食管鳞状细胞癌(esophageal squamous carcinoma,ESCC)组织中的表达并分析二者的临床意义。方法：原位杂交技术和免疫组化方法分别检测80例食管鳞状细胞癌及癌旁正常组织中miR-21和ERK1/2的表达,Spearman相关统计方法分析miR-21和ERK1/2表达的相关性,并分析二者与ESCC患者临床病理资料的关系。结果：miR-21和ERK1/2在ESCC组织中的阳性表达率和表达量都显著高于癌旁正常组织,尤其在有淋巴结转移的ESCC组织中显著增高,但在ESCC不同性别、年龄、民族、病理类型、肿瘤侵及范围和肿瘤大小间的差异无统计学意义。进一步分析发现miR-21与磷酸化的ERK1/2(p-ERK1/2)在ESCC及癌旁正常组织的表达呈正相关,相关系数为0.570。结论：miR-21和p-ERK1/2与ESCC发生发展密切相关,二者可能协同通过促进肿瘤细胞淋巴结转移增加ESCC的恶性程度。     

口腔鳞状细胞癌组织中HPV感染及EGFR、Sox2蛋白表达与肿瘤预后的相关性

目的探讨口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)组织中人乳头状瘤病毒(human papiloma virus,HPV)感染及EGFR、Sox2表达与肿瘤复发的关系。方法收集OSCC组织标本85例,分别采用PCR-DNA反向点杂交及免疫组化SP法检测蜡块组织中HPV感染及EGFR、Sox2表达,收集患者病理资料并进行随访。结果 HPV在OSCC组织中的检出率为29.4%(25/85),阳性患者以非吸烟者居多,高于阴性患者(P0.001)。HPV感染与患者性别、年龄、肿瘤部位、临床分期、分级及淋巴结转移无明显相关性(P0.05);EGFR、Sox2的阳性率分别为69.4%(59/85)、71.8%(61/85);EGFR表达与肿瘤分化程度有关(P0.001);Sox2表达与肿瘤分化程度及TNM分期有关(P0.001、P0.009)。随访2年以上的OSCC患者中17例出现复发(复发组),HPV阳性患者治疗后复发率为5.9%(1/17),低于HPV阴性患者(P=0.037);EGFR、Sox2蛋白在复发组和未复发组中阳性率分别为88.2%、94.1%及64.7%、66.2%;Sox2蛋白在复发组中的阳性率明显高于未复发组(P=0.047)。结论 OSCC中存在一定比例的HPV感染及EGFR、Sox2表达;HPV阳性患者复发率低于阴性患者;EGFR表达与肿瘤分级有关;Sox2表达与肿瘤分级、TNM分期及复发相关。HPV阴性、EGFR及Sox2阳性患者治疗后应定期随诊。     

Overexpression of periostin is significantly correlated to the tumor angiogenesis and poor prognosis in patients with esophageal squamous cell carcinoma

Recent studies have found that periostin (PN), as a kind of secreted glycoprotein, is closely related to the metastatic potential and prognosis of many kinds of tumors. This study aimed to examine the expression of PN in patients with esophageal squamous cell carcinoma (ESCC) and explore the relationship of PN expression with clinicopathologic factors, tumor angiogenesis and prognosis. The results showed that increased PN protein expression was prevalent in ESCC and was significantly associated with lymphatic metastasis (P=0.008), tumor differentiation (P=0.04), venous invasion (P=0.014) and TNM stage (P=0.001). Additionally, expression of PN was found to be an independent prognostic factor in ESCC patients. High expression of PN protein is closely correlated to the tumor progression and angiogenesis and poor survival of ESCC. Taken together, PN is a promising biomarker to identify individuals with poor prognostic potential and concludes the possibility of its use as a prognostic marker in patients with ESCC.     

Mnk2蛋白水平与食管鳞癌预后的相关性

目的:检测丝裂原活化蛋白激酶相互作用激酶2(mitogen-activated protein kinase-interacting kinase-2,Mnk2)在食管鳞状细胞癌中的表达水平,并探讨其与患者生存预后的相关性。方法:收集临床食管鳞癌标本86例及癌旁正常食管组织54例,应用Western blot法和免疫组化SP法检测肿瘤组织及正常食管黏膜组织中Mnk2蛋白表达水平,并用Kaplan-Meier生存曲线和Cox比例风险回归模型的方法探究其与食管鳞癌患者预后的关系。结果:Mnk2在食管癌组织中呈高表达,并且Mnk2蛋白表达与食管鳞癌的TNM分期密切相关(P0.05),同时Mnk2蛋白高表达组的无疾病进展生存期和总生存期均少于Mnk2低表达组,多因素分析提示Mnk2是食管鳞癌的独立预后因子。结论:Mnk2在食管鳞癌组织中的表达与TNM分期有关,同时可作为预测食管鳞癌患者预后的指标。     

Decreased expression of microRNA-20a promotes tumor progression and predicts poor prognosis of cutaneous squamous cell carcinoma

Background: MicroRNA-20a (miRNA-20a or miR-20a) plays a key role in tumorigenesis and progression. But the prognostic value of miR-20a in cutaneous squamous cell carcinoma (CSCC) remains unclear. The aim of this study was to identify the association of miR-20a and the prognosis of CSCC patients. Methods: The miR-20a expression was detected using quantitative real-time polymerase chain reaction (qRT-PCR) in 152 CSCC tissues and matched adjacent normal tissues. Kaplan-Meier and Cox regression analysis were utilized to determine the association of miR-20a with overall survival as well as the prognosis of CSCC patients. Results: The expression of miR-20a was lower in CSCC tissues compared with adjacent normal tissues (P=0.000). Moreover, the expression of miR-20a was closely correlated with TNM stage (P=0.013). Kaplan-Meier analysis showed that patients with low miR-20a expression had significantly poorer overall survival than those with high miR-20a expression (P<0.05). Multivariate analysis revealed that miR-20a expression (P=0.001, HR=3.262, 95% CI: 1.635-6.520) could influence the prognosis and might be an independent prognostic predictor in CSCC. Conclusions: Our results indicated that low miR-20a expression was associated with tumor stage of CSCC and suggested that miR-20a expression would be a novel biomarker for predicting clinical outcomes in CSCC patients. The inhibition of miR-20a might even become a new therapeutic method for the treatment of CSCC.     

Overexpression of CD9 correlates with tumor stage and lymph node metastasis in esophageal squamous cell carcinoma

Objective: Esophageal squamous cell carcinoma (ESCC) is one of the leading causes of cancer deaths worldwide. CD9 has been reported to play a critical role in cell motility, growth and metastasis of multiple cancers. The present study investigated the clinicopathological features of CD9, and its biological characteristics in ESCC. Methods: Fifteen normal esophageal tissue specimens, fifty-three ESCC adjacent tissues and one hundred and four ESCC tissues were included in this study. Using immunohistochemistry (IHC), the expression levels of CD9 were evaluated among different samples. And its clinicopathological parameters and its prognostic factors were analyzed. Western blotting was used to measure CD9 expression and colony formation was performed to determine the effect of CD9 on cell growth in ESCC TE-1 cells. Results: Compared with normal esophageal tissues and tumor adjacent tissues, CD9 expression level is significantly higher in ESCC tissues. CD9 expression correlated with tumor stage (P = 0.022) and lymph node metastasis (P = 0.019) in ESCC patients. Furthermore, the small interfering RNA-mediated silencing of CD9 expression in TE-1 cells resulted in increased proliferation as evidenced by increased colony number and colony size. Conclusion: CD9 expression is upregulated in ESCC tissues and its expression is correlated with tumor stage and lymph node metastasis in ESCC patients. CD9 suppresses the proliferation of TE-1 cells. CD9 may present a potential in tumor progression in ESCC.     

Up-regulation of miR-335 predicts a favorable prognosis in esophageal squamous cell carcinoma

Introduction: MicroRNAs (miRNAs) are noncoding RNAs that regulate multiple cellular processes during cancer progression. MiR-335 has recently been identified to be involved in tumorigenesis of several cancers such as ovarian cancer and gastric cancer. However, the regulation of miR-335 in esophageal squamous cell carcinoma (ESCC) has not been reported yet. Methods: Expression of miR-335 in tumor and their normal matched tissues was determined by quantitative real-time PCR in 67 ESCC patients and its association with overall survival of patients was analyzed by statistical analysis. Results: The expression level of miR-335 was reduced in malignant tissue samples in comparison to normal matched tissue (P < 0.05). It was also proved that miR-335 expression was associated with ESCC histological grade, lymph node metastasis, tumor stage and clinical stage (P < 0.05). In addition, the Kaplan-Meier survival curves revealed that low miR-335 expression was associated with poor prognosis in ESCC patients. Multivariate analysis showed that miR-335 expression was an independent prognostic marker of overall survival of ESCC patients. Conclusions: The study proves for the first time that miR-335 is down regulated in a majority of ESCC patients. Our results indicate that miR-335 expression is an independent prognostic factor for patients with esophageal cancer, which might be a potential valuable biomarker for ESCC.     

PINCH expression and its significance in esophageal squamous cell carcinoma

Particularly interesting new cysteine-histidine rich protein (PINCH), as a newly discovered protein of LIM family members, may play a role in signal transduction of integrin and growth factor, and involved in the incidence and development of tumors. PINCH protein is overexpressed in tumor-associated stroma of several types of tumors. However, there is no study of the PINCH in esophageal cancer, therefore we investigated PINCH expression in esophageal squamous cell carcinomas and its clinicopathological significance in the patients. PINCH expression was immunohistochemically examined in 20 normal esophageal samples and 64 esophageal squamous cell carcinomas. The results showed that PINCH expression in the stroma of cancers was heterogeneous, and its positive rate (56%) was higher than that of normal esophageal mucosa (5%, p<0.0001). The stronger staining was observed at the invasive edge of tumor when compared to the inner area of tumor. The rate of positive PINCH (90%) in the cases with lymph node metastasis was higher than that (41%) in the cases without metastasis (p<0.0001). PINCH expression was not correlated with patients' gender, age, tumor location, size and differentiation (p>0.05). The results suggest that PINCH protein may be a marker of tumor associated-stroma involving tumor development, and predicting the ability of invasion and metastasis of esophageal squamous cell carcinoma.     

P53、P21WAFI、MDM2及BAX在食管鳞癌中的表达和相关性分析

目的：检测P53、MDM2、P21^WAFI、BAX在食管鳞癌中的表达,探讨它们与食管鳞癌临床病理特征之间的关系。方法：采用免疫组化检测食管鳞癌、正常食管粘膜和不典型增生组织中上述基因蛋白的表达。结果：各指标在上述组织中的表达有显著性差异。MDM2在淋巴结转移组中的阳性表达率为80％,未转移组中为35．7％,两者之间有显著性差异。随着食管鳞癌组织学分级的增高,P53、MDM2的表达逐渐增高,P21^WAFI、BAX的表达逐渐降低。结论：P53、P21、MDM2、BAX多个基因的异常在食管癌的发生、发展过程中共同发挥作用;MDM2过表达与肿瘤的侵袭性和转移有关。P53、P21^WAFI、MDM2在食管鳞癌中的表达无相关性,P53与BAX的表达呈负相关。     

Association of p53 expression with prognosis in patients with esophageal squamous cell carcinoma

It has been well accepted that p53 overexpression is associated with advanced stages of cancer. However, the prognostic role of p53 overexpression in esophageal squamous cell carcinoma (ESCC) remains unclear. To investigate the prognostic role of p53 overexpression in patients with ESCC, a retrospective cohort study of 136 ESCC patients was carried out. The expression of p53 protein in tumor tissues was investigated immunohistochemically. Positive expression of p53 protein was detected in 57 ESCC patients (41.9%). The p53 overexpression was associated with smoking (P < 0.001), tumor differentiation (P < 0.001), and tumor size (P < 0.001). In the Kaplan-Meier analysis, patients with p53 overexpression had significantly shorter overall survival than those patients with negative p53 expression (log-rank P < 0.001). Multivariable analysis by Cox regression model further showed that p53 overexpression was a significantly independent predictor of poorer overall survival (hazard ratio [HR] = 1.91; 95% confidence interval [95% CI] 1.03-3.54, P = 0.04). Thus, p53 overexpression is associated with poor prognosis in patients with early stage esophageal squamous cell carcinoma, and it’s a significantly independent predictor of poorer overall survival.     

A nomogram for predicting lymph node metastasis in superficial esophageal squamous cell carcinoma

Superficial esophageal squamous cell carcinoma (SESCC) is defined as carcinoma with mucosal or submucosal invasion, regardless of regional lymph node metastasis (LNM). The lymph node status is not only a key factor to determine the training strategy, but also the most important prognostic factor in esophageal cancer. In this study, we establish a clinical nomogram for predicting LNM in patients with SESCC. A predictive model was established based on the training cohort composed of 711 patients who underwent esophagectomy for SESCC from December 2009 to June 2018. A prospective cohort of 203 patients from June 2018 to January 2019 was used for validation. Favorable calibration and well-fitted decision curve analysis were conducted and good discrimination was observed (concordance index [C-index], 0.860; 95% confidence interval [CI], 0.825–0.894) through internal validation. The external validation cohort presented good discrimination (C-index, 0.916; 95% CI, 0.860–0.971). This model may facilitate the prediction of LNM in patients with SESCCs.