P504s、P63及CK34E12在前列腺良恶性病变中的鉴别意义

目的探讨P504s结合P63、CK34 E12免疫组化在前列腺良恶性中的表达情况及诊断意义。方法收集88例前列腺良恶性病变组织,对其行HE常规染色,并用P504s、P63、CK34 E12作为免疫标记物标记前列腺良恶性组织。结果 P504s在前列腺癌、高级别PIN与良性增生组织间、P63在前列腺癌与高级别PIN、良性前列腺组织间及CK34 E12在前列腺癌与高级别PIN、良性前列腺组织间差异均有统计学意义(均<0.05),P63能在高级别PIN与良性前列腺组织100%表达;CK34 E12在高级别PIN与良性前列腺组织间表达差异无统计学意义(>0.05)。结论 P504s结合P63、CK34 E12对前列腺癌、高级别PIN、良性前列腺组织有鉴别作用。     

免疫组化染色在前列腺穿刺活检中的应用

目的探讨免疫组化染色在前列腺穿刺活检中的应用价值。方法在组织学观察基础上,对321例前列腺穿刺标本进行P504s、P63、CK34βE12免疫组化检测。结果P504s在前列腺癌、前列腺上皮内瘤变(prostatic intraepithelial neoplasia,PIN)和良性增生组织中的阳性表达差异有统计学意义(P<0.01);在不同Gleason评分的前列腺癌组织中,P504s阳性表达差异无统计学意义(P>0.05);P63、CK34βE12在前列腺癌组织中表达呈阴性,在上皮内瘤变中呈不连续表达,在良性增生病变中呈强阳性表达。结论P504s是前列腺癌敏感而特异性标记物,与P63、CK34βE12联合标记在前列腺病变鉴别诊断中具有重要意义,可提高前列腺癌的诊断准确率。     

免疫组化联合检测诊断前列腺良恶性疾病的研究(附36例报告)

目的探讨CK34βE12、P63、P504s联合免疫组化染色在前列腺良、恶性病变鉴别诊断中的意义。方法采用免疫组化方法,观察CK34βE12、P63、P504s在不同前列腺疾病中的表达情况。结果36例前列腺病变中,28例良性前列腺增生及低度的不典型增生(PIN)的腺泡及导管周围可见连续的CK34βE12和P63呈阳性,少数呈间断表达。特别以P63染色明显,但P504s染色皆呈阴性。高度不典型增生(PIN)CK34βE12、P63染色呈不连续表达或阴性,而增生的腺上皮,部分细胞P504s呈弱(+)或(+);非典型腺瘤性增生,CK34βE12、P63呈(+)、P504s(-);前列腺癌8例中7例CK34βE12、P63染色呈(-),1例显色局灶性(+),但P504s均呈(+)。结论P63、CK34βE12、P504s联合检测可明显提高前列腺癌诊断的正确性。     

P504s、P63和Ck34βE12联合检测在前列腺腺癌鉴别诊断中的应用价值

目的探讨P504s、P63和Ck34βE12联合免疫组化染色在前列腺良、恶性病变鉴别诊断中的价值。方法采用免疫组织化学方法观察P504s、P63、Ck34βE12和PSA在82例不同前列腺疾病中的表达情况。结果在18例前列腺腺癌中P63、Ck34βE12染色均(-),17例P504s呈( );良性前列腺增生及低度前列腺上皮内瘤(PIN)的腺泡和导管周围可见连续的P63和Ck34βE12( ),少数呈间断表达,但P504s染色皆呈(-);PIN,P63和Ck34βE12染色基底细胞呈不连续表达或(-);而增生的腺上皮,部分细胞P504s呈弱( );非典型腺瘤性增生P63和Ck34βE12呈( ),P504s呈(-)。PSA在所有病例中均强阳性表达。结论P504s对判断前列腺腺癌具有重要参考价值;P504s、P63和Ck34βE12联合检测可提高前列腺癌诊断的正确率;PSA对前列腺组织有极高的敏感性,但在良、恶性病变鉴别诊断中意义不大。     

组织芯片检测前列腺癌中PSA、P504s、CK34 β E12、P63和Ki-67的表达

目的 利用组织芯片及免疫组织化学染色技术,研究PSA、P504s、CK34βE12、P63、和Ki-67在前列腺疾病中的表达及其在前列腺癌病理诊断中的价值.方法 用24例前列腺疾病(腺癌14例、良性增生8例、平滑肌瘤1例、转移癌1例)的组织芯片做快捷免疫组织化学染色,对以上5个蛋白在组织中的表达水平进行半定量分析,比较了它们在良性前列腺增生和前列腺癌中的表达水平.结果 良性前列腺增生PSA染色全部强阳性,前列腺癌强阳性仅1例(P=0.0001).良性前列腺增生CK34βE12和P63全部阳性,而前列腺癌这两个指标均阴性,两者相比差异有显著性(P<0.05).P504s标记在前列腺癌中均为强阳性,而良性前列腺增生仅1例有表达(P<0.05).前列腺癌Ki-67高表达占57.1%(8/14),显著高于良性前列腺增生(0/8)(P=0.0264).结论 P504s、CK34βE12、P63和Ki-67抗体联合使用,在前列腺癌的病理诊断中有参考价值.P504s阳性可以辅助前列腺癌的诊断,CK34βE12和P63表达可帮助排除前列腺癌变.组织芯片技术能使免疫组织化学成为高通量、经济而准确的检测手段,其在病理学研究和诊断中有重要价值.     

免疫组化在前列腺癌诊断中的作用

目的探讨P504s、P63、CK34βE12在前列腺癌诊断中的价值。方法采用免疫组织化学SP法,对45例血清PSA>4.0μg/L病人,行经直肠前列腺穿刺活检,分别检测P504s、P63、CK34βE12在前列腺癌和良性前列腺增生中的表达。结果 45例前列腺癌中,32例P504s阳性,但P63、34βE12在所有前列腺癌腺体组织中均呈阴性。良性前列腺增生表现为P504s阴性,P63和CK34βE12阳性。结论 P504s和P63、CK34βE12联合检测,可极大地提高前列腺癌诊断的准确率。     

113例前列腺疾病临床病理观察

目的 探讨免疫组化在前列腺疾病病理诊断中的作用.方法 采用免疫组化方法,察PSA、34βE12、p63、p504S在不同前列腺疾病中的表达情况.结果 42例BPH、5例LGPIN、3例HGPIN、8例AAH、7例PAH均有34βE12、p63、PSA不同程度阳性表达;p504S在8例前列腺癌和3例HGPIN中具有较好阳性表达.结论 尽管PSA、34βE12、p63、p504S联合应用可以提高前列腺疾病的诊断正确率,在经尿道切除前列腺和根治性前列腺标本中意义不如在穿刺活检中那样重要.     

P504s和34βE12检测在前列腺病变良恶性鉴别中的意义

目的:探讨免疫组化染色在前列腺穿刺活检中的应用价值.方法:在组织学基础上,对112例前列腺穿刺标本进行P504s、34βE12免疫组化检测.结果:P504s在前列腺癌、前列腺上皮内瘤变(PIN)和良性增生组织中的阳性表达差异有统计学意义(P＜0.01);在不同Gleason评分的前列腺癌组织中,P504s阳性表达差异无统计学意义(P＞0.05);34βE12在前列腺癌组织中表达呈阴性,在上皮内瘤变中呈不连续表达,在良性增生病变中呈强阳性表达.结论:P504s是前列腺癌特异性标记物,与CK 34βE12联合标记在前列腺病变鉴别诊断中具有重要意义,可提高前列腺癌的诊断准确率.     

P504s/P63/34βE12联合双染标记早期诊断前列腺癌的应用研究

目的探讨联合使用P504s、P63、34βE12双染标记前列腺组织标本早期诊断及鉴别诊断前列腺癌的可行性、特异性、敏感性以及临床应用价值。方法使用P504s单抗、P63单抗、34βE12单抗先后在同一前列腺穿刺活检组织标本上行前列腺腺泡细胞和基底细胞免疫组化双重标记染色。P504s表达于癌变的前列腺细胞浆;P63表达于前列腺基底细胞核,34βE12表达于前列腺基底细胞浆。结果 P504s表达阳性而34βE12和P63表达阴性或不连续表达提示为前列腺癌,并可行Gleason评分;P504s表达阳性、34βE12和P63完整阳性表达提示前列腺上皮内瘤;504s表达阴性、34βE12和P63连续阳性表达提示良性前列腺病变。结论通过联合使用P504s、P63和34βE12在同一前列腺标本上进行双重染色标记前列腺癌变细胞、前列腺基底细胞,通过观察前列腺基底细胞的完整性、前列腺癌细胞的形态学表现等可以有效诊断和鉴别前列腺癌、前列腺上皮内肿瘤(PIN)、良性前列腺增生等,增加对前列腺癌的早期诊断率和诊断的准确性,促进临床前列腺癌的早期治疗。     

40例前列腺穿刺组织病理诊断分析

目的探讨穿刺活检对前列腺疾病病理诊断的价值。方法对2014年2月~2015年2月在我院收治的40例疑似前列腺癌患者的临床资料进行回顾性的分析,并对穿刺活检组织进行常规病理学检查分析。结果穿刺活检组织病理检查显示,本组40例患者中前列腺增生症27例,前列腺癌(PC)13例,其中3例高分化腺癌,6例中分化腺癌,4例低分化腺癌。免疫组化法分析显示,13例前列腺癌患者CK34βE12、p63表达均为阴性,P504S表达均为阳性,27例前列腺增生症CK34βE12、p63表达均为阳性,P504S均为阴性,全部病例PSA均为阳性。结论前列腺穿刺组织病理诊断和免疫组化在疾病鉴别诊断中具有较高应用价值,还可用于前列腺癌分化程度评估。     

P504s在前列腺癌中的表达研究

目的探讨P504s和前列腺特异性抗原(PSA)在不同前列腺组织中的表达及其在前列腺癌(PCa)诊断中的价值。方法回顾性分析107例前列腺手术或穿刺活检患者的临床资料,其中前列腺癌(PCa)患者40例,前列腺上皮内瘤(PIN)患者13例,前列腺增生(BPH)患者54例,对存档病理蜡块进行免疫组化染色。结果 PCa、PIN中P504s的表达的阳性率与BPH中P504s的表达的阳性率比较,差异有统计学意义(P0.05),而PCa与PIN中P504s的表达阳性率比较,差异无统计学意义(P0.05)。P504s、PSA与PCa的Gleason分级无明显相关性(P0.05)。结论 P504s具有良好的前列腺组织特异性和肿瘤特异性,在PCa早期诊断及鉴别诊断方面具有重要价值。     

人前列腺癌中P21CIP1/WAF1, Rb的表达意义

目的：研究P21^CIP1/WAF1及Rb在人前列腺癌标本中的表达以及两的相关性,以阐述它们与前列腺癌的病理分级及临床分期的关系。方法：应用免疫组化SABC法染色技术对36例前例腺癌标本进行检测,观察P21^CIP1/WAF1及Rb在前列腺癌各病理分级,临床分期中的表达及相关性。结果：在36例人前列腺癌标本中,P21^CIP1/WAF1和Rb免疫组化阳性染色为棕黄色或棕褐色,分别定位于细胞质和细胞核,其中P21^CIP1/WAF1阳性19例,阳性率52.8%。Rb阳性17例,阳性率47.2%。在不同病理分级、临床分期之间差异均有显性,但实验结果未发现P21^CIP1/WAF1与Rb有显相关性。结论：P21^CIP1/WAF1,Rb的表达与前列腺癌细胞的病理学分级、临床分期呈负相关性,在前列腺癌分子发病机制中可能涉及到P21^CIP1/WAF1,Rb调节通道的异常。     

Urethra, prostate

Questionnaire survey of Chinese urologists concerning the diagnosis and treatment of chronic prostatitis; Clinical value of high grade prostatic intraepithdial neoplasia for predicting prostate cancer; Accuracy of Partin tables to predict final pathological features in prostate cancer of Chinese population     

Urethra, prostate

Diagnostic and differential value of 3D ^1H MR spectroscopy in prostate cancer;Construction and expression of replication-deficient recombinant adenoviral vector carrying prostate specific membrane antigen gene;Transurethral suspendplasma resection of prostate for treatment of benign prostatic hyperplasia patients;Three-dimensional computer reconstruction of male canine prostatic duct system;Clinical pathological study on biologic markers for prostate cancer     

Urethra, prostate

Comparison of treatment planning by carbon ion radiotherapy and by intensity-modulated radiotherapy for prostatic adenocarcinoma, Pedicled rectus abdominis muscle flap for suspending the bulbar urethra in the treatment of male acquired urinary incontinence： an experimental and clinical study, Effect of mifepristone on cell cycle arrest and its regulators in prostate cancer cell line PC- 3, Extraperitoneai laparoscopic radical prostatectomy for prostate cancer and functional recovery of urinary continence, Comparison of intermittent versus continuous androgen blockade in the treatment of advanced prostate eaneer     

Urethra,prostate

<正>209578 Diagnosis and treatment of posterior urethral stricture:twenty-year clincal experience/Zhang Jiong(张炯,Dept Urol 6th Peop Hosp Shanghai Jiaotong Univ,     

Urethra, prostate

Preliminary experience in managing renal ca. lyceal calculi with actively deflectable, flexible ureteroscopy; Primary repair of iatrogenic injury to the ureter; Study of tension-free vaginal tape for surgical treatment of female stress urinary incontinence and prevention of its complications; Cell cycle arrest and apoptosis of androgen independent prostate cancer cell line PC-3 induced by curcumin in vitro; Expression of AMACR and p63 in prostate cancer and its diagnostic value; Effect of nitric oxide on the prostatic prolifer. ation apoptosis in rats;Molecular mechanism underlies LNCaP-flu cell line＇s flutamide insensitivity;……     

Urethra,prostate

<正>209268 Diagnostic value of perfusion weighted magnetic resonance imaging in prostate cancer and benign prostatic hyperplasia/Zhao Xin(Dept Radiol,Tianjin Med Univ Gen Hosp,Tianjin 300052)…∥Chin J Urol.-2009,30(3).-207~209Objective To evaluate the diagnosis and differential diagnostic value of perfusion weighted magnetic resonance imaging for prostate cancer(PCa)and benign prostatic hyperplasia(BPH).Methods Fifty-three cases were divided into 3 groups:group 1 including 23 cases of PCa,group 2 including 22 cases of BPH,and 8 healthy men enrolled in group 3 as normal control.The differences of perfusion related parameters(including rNEI,rMTE and rMSD)among the 3 groups were studied and analyzed.Results In peripheral zone,the value of rNEL in PCa patients was higher than that in BPH patients(t=11.64,P<0.001)and control healthy men(t=11.79,P<0.001).The value of rMTE of PCa patients was lower than that in BPH patients(t=3.50,P=0.002)and control(t=3.78,P=0.001).In central zone,the value of rNEI in PCa patients was higher than that in BPH patients(t=6.99,P<0.001)and control(t=10.32,P<0.001).According to ROC,the rNEI had the best differential diagnostic value.The optimal threshold was 6.44 with sensitivity of 91.3% and specificity of 81.8%for the PCa differentiation with BPH.Conclusion rNEI is and effective parameter to differentiate PCa from BPH.6 refs,2 tabs.     

Urethra,prostate

<正>209429 Clinical significance of differential expression of inflammatory factors in chronic non-bacterial prostatitis/chronic pelvic pain syndrome/Zhou Qing(周青,Dept Trad Chin Med Surg,1st Affil Hosp,Human Univ TCM,Changsha 410007)…∥Chin J Urol.-2009,30(6).-386～389Objective To investigate the role of inflammatory cytokines in the pathogenesis of chronic non-bacterial prostatitis/chronic pelvic pain syndrome(CAP/CPPS)patients.Methods The 38 cases with CAP/CPPS patients(18 cases of CAP and 20 cases of CPPS)and 20 cases of healthy controls were selected.The differential expressions of 40 kinds of inflammatory cytokines were detected by antibody arrays in prostate fluid.Results The inflammatory cytokines which increased more than1.5 times expression were found.There were seven kinds in CAP including monocyte chemoattractant protein(MCP)-1,solution tumor necrosis factor receptor Ⅱ(s TNF R Ⅱ),platelet-derived growth factor-BB(PDGF-BB),interleukin(IL)-1β,IL-11,IL-6,MCP-2 and five kinds in CPPS groups including MCP-1,PDGF-BB,MCP-2,s TNF R Ⅱ,IL-11 respectively,compared with healthy control group.The cluster analysis results showed that protein expression of Monocyte chemoattractant protein 1(MCP-1)and platelet-derived growth factor BB(PDGF-BB)were significantly increased in CAP(3.47 and 2.07 times)and CPPS(2.25 and 2.19 time)compared with helathy control group and were the final polymerization of inflammatory cytokines.The protein expression of interleukin 1 β(IL-1β),MCP-1 and soluble tumor necrosis factor Ⅱ(s TNF R Ⅱ)in CAP group was increased more than 1.85,1.55,1.67 times compared with CPPS group.Conclusion Elevated expression of inflammatory cytokines may play an impotant role in the course of CAP/CPPS disease.The extent of the inflammatory response of CAP was higher than that of CPPS.The inflammatory factors of MCP-1 and PDGF-BB could serve as a novel diagnostic marker.12 refs,2 tabs.     

Urethra, prostate

206644Different effects of various mutants of PAK6kinase on the proliferation of prostate cancer cells/ WenXingqiao(温星桥,Dept Urol ,3rd Affil Hosp,Sun Yat-sen Univ,Guangzhou510630)…∥ChinJ Urol .-2006 ,27(8) .-552 ~555ObjectiveTo evaluate the effects of various mu-tants of PAK6 kinase onthe proliferation of prostate can-cer cells and their roles in the pathology of prostate can-cer .MethodsVariant mutants of PAK6 kinase ,includ-ing wildtype ( WT) ,kinase activated type (SN) and k…