准分子激光角膜原位联合角膜瓣背面磨镶术治疗LASIK术后屈光回退

目的 探讨准分子激光角膜原位联合角膜瓣背面磨镶术治疗LASIK术后屈光回退的有效性和安全性.方法 对10例(15眼)LASIK术后屈光回退,剩余的角膜基质层厚度不足以矫正的患者行准分子激光角膜原位联合角膜瓣背面磨镶术治疗.结果 术后复查角膜瓣平整、复位良好.术后视力第1天、1周、3个月均值分别为0.95±0.26、0.98±0.25、1.02±0.26;术前屈光度(-2.5±1.25)DS,散光(-1.05±0.65)DC;术后3个月的屈光度(-0.37±0.50)DS,散光(-0.50±0.37)DC,术后与术前相比差异有统计学意义(F=6.250,P=0.002;F=3.086,P=0.043),术后3个月时15眼均达到或超过术前最佳矫正视力.结论 准分子激光角膜原位联合角膜瓣背面磨镶术可以安全有效地治疗LASIK术后屈光回退、术前预测角膜基质层厚度不够的近视患者,而且无明显并发症,但远期疗效还有待进一步观察.     

准分子激光原位角膜磨镶术治疗近视的临床研究

目的观察准分子激光原位角膜磨镶术(LASIK)治疗近视的临床疗效。方法对1 000只眼LASIK术前与术后的视力、屈光度进行比较、分析、随访。结果中低度组患者在术后随访6个月后视力1.04±0.22,屈光度 0.43±0.15,高度组视力0.84±0.36,屈光度0.25±0.76;超高度组视力0.54±0.76,屈光度-2.25±1.24。结论准分子激光原位角膜磨镶术(LASIK)具有安全、有效、稳定等特点,逐渐得到广大近视患者的接受,成为角膜屈光手术的主导术式。     

准分子激光原位角膜镶磨术治疗近视患者80例效果观察

目的探讨准分子激光原位角膜镶磨术治疗近视的临床效果,为近视的治疗提供方法参考。方法将近视患者80例分为低度近视组10例,中度近视组30例,高度近视组40例,三组均采用准分子激光原位角膜镶磨术治疗。结果术前三组眼压值、角膜厚度、屈光度无明显差异;经过治疗后都有明显下降,其中低度组与中度组、低度组与高度组、中度组与高度组,手术前后眼压、角膜厚度变化有统计学意义（P〈0.05）;术后三组间屈光度对比差异无统计学意义（P〉0.05）。结论准分子激光原位角膜镶磨术治疗不同程度的近视都能取得很好的治疗效果,能降低眼压、角膜厚度与屈光度,值得推广应用。     

准分子激光原位角膜磨镶术治疗近视的临床观察

目的：观察准分子激光原位角膜磨镶术（LASIK）治疗近视的临床疗效。结论：准分子激光原位角膜磨镶术（LASIK）具有安全、有效、稳定等特点,适合治疗各种不同屈光度的近视,超高度近视由于受角膜厚度的限制,其疗效稍差。     

LASEK与LASIK术后角膜后表面形态临床观察

目的比较准分子激光上皮瓣下角膜磨镶术(laser subepitheliar keratomileusis, LASEK)术后与准分子激光原位角膜磨镶术(laser in situ keratomileusis, LASIK)后角膜后表面形态的变化。方法将134例268眼近视患者分为两组,其中接受LASEK治疗的为1组79例158眼,平均屈光度( -5.67±0.59)D,接受LASIK治疗的为2组55例110眼,平均屈光度( -5. 97±0.83)D。术后采用orbscan -2角膜地形图系统随访6个月。结果术后6个月时,1、2组的角膜中央3mm区后表面屈光力和角膜后顶点到理想球面之间的距离(diff值)的改变量,2组相比差异有显著性(P<0.01)。结论 LASEK与LASIK术后角膜表面均有不同程度的前突且与残留角膜床厚度相关。LASEK在角膜后表面形态的稳定性方面优于LASIK。     

LASIK术后眼压测量值的改变及影响因素

目的探讨准分子激光原位角膜磨镶术（LASIK）后影响眼压变化的因素。方法 181例近视患者均行LASIK。术前、术后3个月分别测量患者眼压、角膜中央厚度、角膜前表面曲率及进行医学验光,并进行比较,对眼压的改变与性别、年龄、术前眼压、术前角膜前表面曲率、术前等效球镜屈光度、术前角膜中央厚度、术中角膜切削深度、术前术后角膜曲率的差值进行相关分析。结果 181例患者LASIK术后3个月眼压、角膜中央厚度、角膜前表面曲率均明显低于术前（P〈0.05）;眼压改变与角膜中央厚度、切削深度、角膜曲率差、术前等效球镜屈光度、术前眼压呈线性正相关;与性别、年龄及术前角膜前表面曲率无相关性。结论 LASIK术后眼压测量值的下降与术前角膜中央厚度、切削深度、术前术后角膜曲率差、术前等效球镜屈光度、术前眼压相关。     

LASIK术后角膜地形图变化及临床疗效观察

目的 探讨准分子激光原位角膜磨镶术(Laser in situ keratomileusis,LASIK)治疗近视前后角膜地形图的变化及其与临床效果和术后并发症的关系.方法 对131例(250只眼)近视患者行LASIK术,术前及术后1月行视力、屈光度、角膜地形图及裂隙灯显微镜检查.结果 LASIK术后视力100%较术前提高;术后角膜地形图图形以平滑型(64.5%)为主;中央岛和偏心切削发生率分别为6.29%、5.03%;SimK值较术前显著降低,降低幅度与切削度数有关;PVA值与术前相比显著降低,SRI和SAI值较术前显著增高.结论 LASlK手术是目前治疗近视的最有效的方法之一,角膜地形图可帮助设计手术方案,判断手术效果,为处理术后并发症和再次补充矫正提供依据.     

准分子激光原位角膜磨镶术前后非接触眼压测量值变化的分析

目的　探讨准分子激光原位角膜磨镶术 (laserinsitukeratomileusis,LASIK)前后非接触眼压计 (noncontacttonommeter,NCT)测量值的变化及其影响因素。方法　对 181例随访时间 >6月的LASIK手术患者资料进行回顾 ,分析NCT测量值变化及其与中央角膜厚度 (centralcornealthickness ,CCT)、屈光度的关系。结果　LASIK后NCT测量值下降 ,差异有非常显著性意度 (P <0 .0 1) ,术后NCT测量值下降与术前屈光度、角度切削深度有关 (P <0 .0 1)。结论　LASIK术后NCT测量值降低 ,屈光度高、角膜切削深度大 ,眼压值下降多。对LASIK术后患者眼压 (intracocularpressure ,IOP)评估必须进行校正。     

准分子激光原位角膜磨镶术前后非接触眼压的影响因素

准分子激光原位角膜磨镶术(LASIK)治疗近视由于安全、可靠及术后无痛苦而被广泛接受.随着手术的大量开展,手术引起的角膜厚度、角膜屈率、眼压等的变化也逐渐引起人们的重视.作者采用非接触式眼压计测量了164例近视患者LASIK手术前后的眼压,并分析其影响因素,报道如下.     

LASIK术后非接触式眼压计测量值的变化

目的 探讨准分子激光原位角膜磨镶术(LASIK)后影响眼压测量值的因素.方法 对实施准分子激光原位角膜磨镶术(LAISK)的159例患者,于术前和术后6个月分别以非接触式眼压计测量眼压,分析眼压变化的原因.结果 LASIK术后眼压测量值明显低于术前,差异有统计学意义.结论 LASIK术后眼压明显下降,这与激光切削角膜变薄有关.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.