Effect of diquertin on platelet content of cyclic nucleotides

The effects of diquertin on the content of cyclic nucleotides in human platelets was studied. Diquertin in a concentration of 5 mM increased the content of cAMP and cGMP in native and thrombin-activated platelets probably due to inhibition of phosphodiesterase. Increasing the concentration of diquertin above 5 mM did not potentiate this effect. The antiaggregation effect of diquertin was probably associated with the increase in platelet levels of cyclic nucleotides. Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny,Vol. 128, No. 9, pp. 267–269, September, 1999     

Content of prostaglandins and cyclic AMP in rat tissue

The content of prostaglandins (PG) and cyclic adenosine-3,5-monophosphate (cyclic AMP) in rat tissues was studied by a radioisotope method. The maximal content of both PG and cyclic AMP was found in the same organs, those functioning most actively: the brain, endocrine glands, and small intestine, and the minimal content in adipose tissue. The investigations show close functional interdependence between PG synthesis and adenylate cyclase activity in the tissues of the body.Kiev Scientific-Research Institute of Endocrinology and Metabolism. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 83, No. 4, pp. 416–418, April, 1977.     

Effect of low-intensity infrared laser radiation on ultrastructure and proliferation of liver cells in experimental hepatitis and cirrhosis

Laboratory of Pathological Anatomy, Tashkent Branch, All-Union Research Center for Surgery, Academy of Medical Sciences. (Presented by Academician D. S. Sarkisov, Academy of Medical Sciences.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 113, No. 4, pp. 424–427, April, 1992.     

Investigation of chloramphenicol acetyltransferase formation byEscherichia coli K-12 cells after changes in intracellular cyclic AMP concentration

The effect of cylic AMP, ACTH, and glucose on formation of the enzyme chloramphenicol acetyltransferase by cells ofEscherichia coli CSH-2/R222 andE. coli WZ-78/R222 (cya₈₅₅) was investigated. Glucose was shown to reduce synthesis of the enzyme inE. coli CSH-2/R222 by inducing catabolite repression; this could be overcome by 5 mM cyclic AMP and 1000 g/ml ACTH. Synthesis of the enzyme inE. coli WZ-78/R222 was resistant to catabolite repression and ACTH did not stimulate the synthesis of chloramphenicol acetyltransferase by this strain.Researh Laboratory of Experimental Immunobiology, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR N. N. Zhukov-Verezhnikov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 81, No. 3, pp. 294–295, March, 1976.     

Effect of melatonin on cyclic nucleotide content and intensity of lipid peroxidation in the hippocampus and habenula of rats exposed to acute hypoxia

Single intraperitoneal injection of melatonin in a dose of 1 mg/kg prevented accumulation of cGMP and intensification of lipid peroxidation in the hippocampus and habenula of rats exposed to acute hypobaric hypoxia (12,000 m). Changes in habenular content of cGMP suggest that melatonin prevents hypoxia-induced activation of heme-oxygenase. Translated fromByulleten' Eksperimental" noi Biologii i Meditsiny, Vol. 130, No. 8, pp. 168–171, August, 2000     

Fluorimetric determination of histamine and serotonin in mast cells of normal and germfree rats treated with reserpine and cyclic AMP

The content of histamine (by the reaction with orthophthalic aldehyde) and serotonin (by Falk's reaction) in mesenteric mast cells of normal and germfree rats was determined microspectrometrically. Reserpine (10 mg/kg, intramuscularly) was shown not to change the histamine content, whereas the serotonin content was reduced by 50%. Injection of cyclic AMP (3.5 mg/kg, intraperitoneally) caused the serotonin content to be increased by 80% and the histamine content by 280–320%.Institute of General Pathology and Pathological Physiology, Academy of Medical Sciences of the USSR. Research Laboratory of Experimental Biological Models, Academy of Medical Sciences of the USSR, Moscow. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny. Vol. 83, No. 2, pp. 175–177, February, 1977.     

Role of prostaglandins and cyclic nucleotides in the mechanism of development of drug-induced thrombocytopenia

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 115, N ^o 4, pp. 354–356, April, 1993     

Content and metabolism of cyclic AMP at different stages of growth of hepatoma 22a

The intracellular content of cyclic 3,5-adenosine monophosphate (AMP) and activity of adenylate cyclase and cyclic AMP phosphodiesterase were determined in the lag period and the exponential and stationary phases of growth of mouse hepatoma 22a. In the stage of transition of the tumor cells from the lag period to the exponential phase of growth, the intracellular cyclic AMP concentration was found to be reduced by half as the result of sudden activation of phosphodiesterase. Later the cyclic AMP content fell more slowly until the cells entered the stationary phase of growth. Since adenylate cyclase activity remained unchanged during growth of the hepatoma 22a, this suggests that an increase in phosphodiesterase activity is the signal for escape of the tumor cells from the resting period and their entry into the mitotic cycle.Laboratory of Biochemical Pharmacology, Oncologic Scientific Center, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR L. M. Shabad.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny Vol. 84, No. 10, pp. 472–474, October, 1977.     

Arginine-vasopressin effect on the content of cyclic nucleotides, cellular protein phosphorylation, and adhesion to plastic of peritoneal mouse macrophages

Intracellular concentration of cAMP and phosphorylation of 15 kD protein in cultured mouse peritoneal macrophages are increased in the presence of arginine-vasopressin. Vasopressin, decreases adhesion of macrophages to plastic. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 7 pp. 80–83, July 1998     

Effect of laser radiation proliferative activity of cultured cells

Using morphometry and radioautography methods, it is found that a single laser irradiation (0.5 and 0.05 J/cm²) of cultured human fibroblasts stimulates their proliferationin vitro. The effect of the laser is realized via intensification of DNA synthesis. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, Nno 3, pp. 313–315, March, 1994 Presented by D. S. Sarkisov, Member of Russian Academy of Medical Sciences     

Investigation of the mechanism of action of ouabain and cyclic AMP of the transport of calcium ions by rat heart mitochondria

The action of ouabain and cyclic AMP on the Ca-accumulating capacity and outflow of Ca²⁺ ions from loaded rat heart mitochondria was studied by the tetracycline probe method. In the course of the investigations no effect of ouabain on these processes was found. Cyclic AMP did not act on Ca binding by the mitochondrial membrane but it induced rapid liberation of Ca²⁺ from organelles loaded with these ions.Department of Molecular Pharmacology, Medico-Biological Faculty, N. I. Pirogov Second Moscow Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR S. S. debov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 83, No. 2, pp. 158–160, February, 1977.     

Effect of He−Ne laser radiation on the amount of ATF in HeLa cells

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 115, N ^o 6, pp. 617–618, June, 1993     

Antioxidant and immunomodulating effects of ceruloplasmin in experimental influenza infection

Exogenous ceruloplasmin is shown to increase the resistance of mice to influenza virus, reduce the immunodepressive effect of the virus, and improve the biochemical parameters in the acute period of experimental infection. A possible positive effect of ceruloplasmin on delayed complications of influenza is discussed. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 9, pp. 285–287, September, 1994 Presented by T. T. Berezov, Member of the Russian Academy of Medical Sciences     

Effect of uridine derivatives on myocardial stunning during postischemic reperfusion of rat heart

Uridine and uridine-5’-monophosphate prevent myocardial stunning during postischemic reperfusion of isolated rat heart. Uridine-5’-diphosphate does not prevent postischemic myocardial dysfunction, while uridine-5’-triphosphate aggravates it. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 10, pp. 411–414, October, 2000     

Effect of cyclic nucleotides and of isopropylnoradrenalin on the partial pressure and consumption of oxygen

Isopropylnoradrenalin, cyclic AMP, and dibutyryl-3,5-AMP (DB) lower the partial pressure of oxygen (pO₂) in the liver and spleen and increase the oxygen consumption of the body. The time course of these two effects for isopropylnoradrenalin and cyclic AMP correlates closely. The increase in heat production was not accompanied by any significant change in the respiratory quotient. The effects of cyclic AMP were not abolished by pempidine or by dihydroergotamine and were only reduced to some degree by propranolol. The effect of catecholamines on pO₂ is evidently mediated through an increase in the O₂ consumption as a result of the effect of cyclic AMP; the action of cyclic AMP is basically direct, but in vivo it cannot take place through the adrenoreceptor; the compound 2,3-AMP reduces pO₂ and also the O₂ consumption.Department of Biochemistry, Krasnoyarsk Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR S. S. Debov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 83, No. 2, pp. 177–180, February, 1977.     

Effect of adrenalin,hydrocortisone, insulin,and dibutyryl-cyclic AMP on glycolysis and glycogenolysis in surviving liver splices from albino rats

Adrenalin, hydrocortisone, and dibutyryl-cyclic AMP inhibited glycolysis and glycogenolysis in surviving liver slices from albino rats. An inhibitory effect also was found when glucose-6-phosphate (G6P), but not fructore-1, 6-diphosphate, was used as the substrate for glycolysis. This indicates that activity of hexokinase and also, probably, of, phosphorylase and phosphofructokinase, in inhibited under the influence, of these hormones and dibutyrul-cyclic AMP. In a reconstituted cell-free system the hormone had no effect and dibutyryl-cyclic AMP inhibited hexokinase only. For the hormones to exert their effect interaction between them and the cell membrane was essential. Inhibition of glycogen and G6P breakdown to lactic acid in liver slices is not connected with the action of the hormones on the corresponding enzymes (phosphorylase and phosphofructokinase) directly through cyclic AMP and protein kinase. The results are evidence of an additional mechanism modifying the action of cyclic AMP on the activity of the above-mentioned enzymes. Insulin had no, effect in all cases.Laboratory of Biochemistry, Institute of Clinical and Experimental Medicine, Siberian Branch, Academy of Medical Sciences of the USSR, Novosibirsk. (Presented by Academician of the Academy of Medical Sciences of the USSR V. P. Kaznacheev.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 86, No. 11, pp. 541–544, November, 1978.