超高频超声评价己酮可可碱治疗大鼠非酒精性脂肪性肝炎

目的探讨己酮可可碱治疗大鼠非酒精性脂肪性肝炎的作用。方法68只大鼠随机分为对照组(18只)、模型组(30只)及治疗组(20只),分别于第8周、12周连续4周给予已酮可可碱(PTX)治疗,并在相对应时期进行超声及病理检查,对测量的相关指标进行比较。结果8周模型组肝细胞出现脂肪样变为单纯性脂肪肝,在此基础上12周出现炎性细胞浸润及坏死为中-重度脂肪肝;16周进一步加重,从脂肪性肝炎进展到脂肪性肝纤维化。各组间比较具有显著性差异(P<0.05)。治疗组超声及病理学检查指标均较对照组有明显减轻但无统计学差异。结论己酮可可碱对大鼠非酒精性脂肪性肝炎具有治疗作用。     

己酮可可碱抑制大鼠非酒精性脂肪性肝炎肝细胞的凋亡

目的 探讨凋亡相关基因在非酒精性脂肪性肝炎(NASH)发生发展中的作用机制,以及己酮可可碱(PTX)对非酒精性脂肪性肝炎(NASH)大鼠的抗凋亡作用. 方法 SD大鼠68只,标准饲料喂养1周后,随机分为8组:8周对照组(6只),8周模型组(10只),12周对照组(6只),12周模型组(10只),12周治疗组(10只),16周对照组(6只),16周模型组(10只),16周治疗组(10只).用高脂饮食法建立大鼠非酒精性脂肪性肝炎模型,腹主动脉取血检测血浆谷丙转氨酶(ALT)、谷草转氮酶(AST)水平,采用免疫组织化学的方法检测肝Bcl-2、Bax、Caspase-3蛋白的表达情况,应用RT-PCR检测Caspase-3 mRNA的转录情况. 结果 各模型组ALT、AST均高于对照组,且模型组的ALT、AST逐渐增高.免疫组织化学实验结果表明,与同周数的对照组相比,各周模型组Bcl-2、Bax、Caspase-3的表达均有显著性增加(P<0.05),与同周数的模型组相比,12周、16周PTX治疗组Bcl-2、Bax、Caspase-3的蛋白表达有显著的降低(P<0.05). 结论 细胞凋亡是NASH的一个重要发病机制,己酮可可碱对NASH大鼠肝脏细胞凋亡有一定的抑制作用.     

己酮可可碱对非酒精性脂肪性肝炎大鼠肝NF-κB活性作用的研究

目的探讨己酮可可碱(PTX)对非酒精性脂肪性肝炎(NASH)大鼠肝核因子-κB(NF-κB)蛋白的活性作用。方法SD大鼠40只,标准饲料喂养1周后,随机分为4组:对照组、12周模型组、16周模型组和PTX治疗组。用高脂饮食法建立大鼠非酒精性脂肪性肝炎模型,采用免疫组织化学染色法检测大鼠肝组织NF-κB和κB抑制蛋白α(IκB-α)的表达,应用Western blotting的方法检测肝组织中NF-κB的表达。结果免疫组织化学及Westernblotting结果表明,与对照组相比,模型组及PTX治疗组大鼠肝NF-κB的表达显著增加(P<0.05),IκB-α显著降低(P<0.05)。与16周模型组相比,PTX治疗组NF-κB的表达有所降低,IκB-α有显著提高(P<0.05)。结论PTX能抑制大鼠肝IκB的降解,减少NF-κB的活化。     

丹参注射液对大鼠非酒精性脂肪性肝炎肝细胞NF-κB表达的影响

目的研究肝细胞核因子-κB（NF-κB）在非酒精性脂肪性肝炎中的作用,并探讨丹参注射液对大鼠非酒精性脂肪性肝炎的保护机制。方法采用高脂饮食16周建立大鼠非酒精性脂肪性肝炎模型。并用丹参注射液5ml／（kg·d）进行干预治疗,观察其对非酒精性脂肪性肝炎大鼠肝细胞核因子-κB表达及病理变化的影响。结果丹参治疗组血清ALT、AST分别为（87．6±13．4）、（160．7±32．5）U／L,较模型组的（102．1±31．1）、（210．3±30．2）U／L明显降低,差异均有统计学意义（P〈0．05）。模型组肝组织出现重度脂肪变性,不同程度的炎细胞浸润及坏死,丹参治疗组肝组织炎症程度较模型组明显减轻,丹参组炎症计分（4．85±0．39）,模型组炎症记分（6．30±0．51）（P〈0．05）。丹参治疗组NF-κB表达为（1．77±1．07）,较模型组的（5．63±1．45）显著降低,差异有统计学意义（P〈0．05）。结论肝细胞核因子．xB表达增强与非酒精性脂肪性肝炎的发生密切相关,丹参注射液能抑制肝细胞核因子．KB表达而达到治疗非酒精性脂肪性肝炎的目的。     

罗格列酮对非酒精性脂肪性肝炎小鼠肝组织肿瘤坏死因子α表达的作用

探讨过氧化物酶体增殖物激活受体γ(peroxisome proliforator-activated receptor-γ,PPAR-γ)激动剂罗格列酮对非酒精性脂肪性肝炎(non-alcoholic steatohepatitis, NASH)小鼠肝组织肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)表达的影响及其在NASH进展中的作用.选取健康雄性C57BL/6J小鼠15只,随机分为3组:对照组、模型组和罗格列酮干预组.酶法检测小鼠血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)和甘油三酯(triglyceride,TG)水平.HE染色光镜下观察肝组织切片脂肪变程度、炎症活动度和纤维化程度.采用RT-PCR和Western印迹法检测TNF-α mRNA和蛋白的表达.结果表明:HE染色显示对照组小鼠肝脏未出现明显脂肪变、炎症和纤维化,而模型组肝脂肪变及炎症明显,罗格列酮干预组上述病变减轻.肝组织TNF-α mRNA及蛋白表达依次为模型组>罗格列酮干预组>对照组(TNF-α mRNA和蛋白条带吸光度值依次为:1.11±0.01、0.99±0.02、0.91±0.00和0.99±0.01、0.60±0.01、0.47±0.01,P＜0.01).结论:在高脂、胆碱-蛋氨酸缺乏饮食(high fat, methionine and choline deficienct diet, MCD)诱导的小鼠NASH模型中,肝组织TNF-α的表达与NASH的进展有关;PPAR-γ特异性激动剂罗格列酮可抑制TNF-α的表达,阻止NASH的进展,为NASH的靶向性治疗药物的选择提供了新思路.     

Nrf2减轻小鼠非酒精性脂肪性肝炎

目的研究Nrf2对小鼠非酒精性脂肪性肝炎(NASH)的作用及其可能机制。方法通过蛋氨酸-胆碱缺乏饲料(MCD)饲喂小鼠获得NASH模型;随机分为对照组、模型组以及Nrf2组,Nrf2组灌胃姜黄素0.9 mL/d,对照组和模型组给予相应体积的0.9%氯化钠注射液灌胃。4周后检测肝组织中Nrf2含量,肝指数,血清谷丙转氨酶(ALT)、谷草转氨酶水平(AST)、葡萄糖(GLU)、三酰甘油(TG)和胆固醇(Chol)含量;对肝脏进行油红O染色;检测肝组织中TG、Chol、丙二醛(MDA)和谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)的活性;并检测细胞色素C和ATP含量。结果与模型组小鼠相比,Nrf2组小鼠的血清中ALT,AST和GLU含量下降(P<0.05),肝组织中TG、Chol和MDA含量下降(P<0.05),GSH水平、SOD活性升高(P<0.05);同时细胞色素C漏出降低,ATP含量上升(P<0.05)。结论 Nrf2对由蛋氨酸-胆碱缺乏所引起的NASH具有一定的保护作用,其机制可能是通过减少氧化应激,提高线粒体活性实现的。     

红景天苷对非酒精性脂肪性肝炎大鼠肝组织氧化应激的抑制作用

 目的：观察红景天苷（salidroside ,SDS）对大鼠非酒精性脂肪性肝炎（NASH）肝组织氧化应激的影响。方法：以高脂高胆固醇饮食14周诱导建立大鼠NASH模型,药物干预组在造模第8周末时给予SDS 300 mg·kg-1·d-1体重灌胃连续6周,在14周末检测大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆固醇（TC）和甘油三酯（TG）,以及肝组织TC、TG、丙二醛（MDA）、超氧化物歧化酶（SOD）和谷胱甘肽（GSH）含量的变化,ELISA检测8-异前列腺素F2α(8-iso-PGF2α)的水平变化, HE染色观察肝组织病理学变化,免疫组化观察8-羟基脱氧鸟苷酸（8-OHdG）表达的变化。结果：在14 周末, NASH模型组大鼠肝组织病理学检查显示肝组织呈中重度脂肪变性并同时伴有炎症细胞浸润;与正常对照组相比,NASH模型组大鼠血清ALT、AST、TG和TC,以及肝TG、TC和MDA的含量显著上升,而肝SOD和GSH的含量显著下降,8-iso-PGF2α的水平显著上升,免疫组化显示8-OHdG表达的阳性细胞数显著增多。与模型组相比,SDS药物干预组大鼠ALT、AST、TG、TC、MDA和8-iso-PGF2α的含量均显著下降,而肝SOD和GSH的含量显著上升,肝病理学变化得到显著改善,8-OHdG表达的阳性细胞数明显减少。结论：SDS对高脂高胆固醇饮食诱导的NASH有较好的抑制效果,其机制可能与SDS的抗氧化作用有关。     

罗格列酮抑制非酒精性脂肪性肝炎大鼠肝组织IKK-β的表达

目的探讨罗格列酮对非酒精性脂肪性肝炎(NASH)大鼠肝组织中IκB激酶β(IKK-β)表达的影响及意义。方法健康雄性Wistar大鼠高脂饲料喂养16周建立NASH模型,应用不同剂量罗格列酮灌胃治疗,并继续高脂饲料喂养12周后空腹收集大鼠血清及肝组织,ELISA检测血清TNF-α水平,RT-PCR和免疫组化染色观察大鼠肝组织IKK-βmRNA、蛋白的表达。结果模型组大鼠血清TNF-α水平显著增高,肝组织IKK-βmRNA及蛋白表达明显增强(P<0.01),肝组织有不同程度的脂肪变性、炎症坏死和纤维化。罗格列酮治疗组上述变化显著缓解,且与罗格列酮剂量正相关。结论罗格列酮可以阻止NASH大鼠IKK-β的表达和TNF-α的生成,抑制肝脏炎症反应。     

内毒素诱发大鼠非酒精性脂肪性肝炎和胰岛素抵抗

目的:观察多次皮下注射小剂量内毒素是否能诱导大鼠发生非酒精性脂肪性肝炎(NASH)与胰岛素抵抗(IR)。方法:将24只大鼠随机分为4组:Ⅰ组为4周正常组;Ⅱ组为4周内毒素组;Ⅲ组为9周正常组;Ⅳ组为9周内毒素组;测定血浆丙氨酸氨基转移酶(ALT)、白细胞介素-10(IL-10)、脂联素(APN)、肿瘤坏死因子-α(TNF-α)、内毒素(ET)、空腹胰岛素(FINS)、空腹血糖(FPG)水平,计算胰岛素抵抗指数(IRI),取肝组织进行HE染色和苏丹Ⅳ染色,以确定是否发生了非酒精性脂肪性肝炎与胰岛素抵抗。结果:4周内毒素组与4周正常组相比,血浆ALT、TNF-α、FINS、ET有升高趋势,IL-10有下降趋势,但均无显著差异(P0.05)。9周内毒素组与9周正常组比较,血浆TNF-α、FINS、FPG、ET以及IRI明显升高,IL-10明显降低且均有显著差异(P0.05);但ALT仅有升高趋势而无显著差异(P0.05),APN亦无明显差异(P0.05)。结论:多次皮下注射小剂量内毒素可使大鼠发生非酒精性脂肪性肝炎与胰岛素抵抗,这提示非酒精性脂肪性肝炎与胰岛素抵抗的发生可能与内毒素密切相关。     

柴芪汤对非酒精性脂肪肝大鼠模型血清TNF-α和IL-6表达的影响

目的:观察柴芪汤对非酒精性脂肪肝(non-alcoholic fatty liver disease,NAFLD)大鼠炎症因子TNF-α和IL-6的影响,探讨其延缓NAFLD进展的作用机制.方法:SPF级雄性SD大鼠40只,随机分为空白组、模型组和治疗组,高脂饲料喂养造模,8周后,空白组与模型组各取8只处死取材,评价模型.造模成功后治疗组每天给予柴芪汤5.67 g/kg灌胃,8周后,处死各组动物,检测血清AST,ALT,TG,TC,LDL-C,HDL-C,TNF-α及IL-6水平,空腹血糖(fasting blood glucose,FBG)及胰岛素(fasting insulin,FINS)水平,评价胰岛素抵抗指数(insulin resistance index,IRI);取肝组织,镜下观察病理形态.结果:高脂饲养8周后,模型组大鼠血清ALT,AST,FBG,FINS及IRI水平高于空白组,差异有统计学意义(P<0.05),可见肝细胞脂肪变性;柴芪汤治疗8周后,大鼠血清ALT,AST,TG,TC,LDL-C,TNF-α及IL-6水平低于模型组,差异有统计学意义(P<0.05),肝细胞脂肪变性程度较模型组轻,肝细胞坏死及炎性细胞浸润不明显.结论:高脂饲养8周后,NAFLD大鼠模型制备成功;柴芪汤可减轻NAFLD大鼠模型肝脏炎症反应和肝细胞脂肪变性程度,下调炎性因子TNF-α和IL-6的表达,降低血清TG,TC及LDL-C,改善胰岛素抵抗.     

姜黄素治疗大鼠脑出血后血清IL-6、IL-1β、TNF-α变化的实验研究

目的 本研究旨在探索大鼠脑出血(intracerebral hemorrhage,ICH)用姜黄素治疗后血清IL-6、IL-1β、TNF-α变化情况,评价姜黄素在治疗脑出血中的抗炎价值,从而为临床应用姜黄素治疗脑出血提供科学的实验数据.方法 24只健康雄性成年SD大鼠,单纯随机分为术前正常对照组(A组),脑出血姜黄素治疗组(B组),脑出血空白对照组(C组),假手术组(D组),每组6只大鼠.立体定位下右侧基底节注射胶原酶法制备大鼠脑出血模型.于建模成功后6h、24h、48h、3d、5d、7d、14d,给各组大鼠尾静脉取血测IL-6、IL-1β、TNF-α.将B组与C组、D组的IL-6、IL-1β、TNF-α值行两样本t检验比较,P＜0.05为差异有统计学意义.结果 B组的神经生物学评分较相应时期的C组低,较相应时期的D组高,差异有统计学意义.B组的血清IL-6、IL-1β、TNF-α水平在术后6h即升高,3d达最高水平,随后开始逐渐恢复,且各个时期的血清IL-6、IL-1β、TNF-α水平均较同时期C组低,较同时期的D组高,差异均有统计学意义.结果 姜黄素能加快大鼠脑出血后血清炎症因子的吸收,对大鼠脑出血有抗炎作用.     

Changes in intramuscular cytokine levels during masseter inflammation in male and female rats

The present study was conducted to examine cytokine profiles in the masseter muscle before and after complete Freund's adjuvant (CFA)-induced inflammation and possible sex differences in the cytokine levels. Age matched male and female Sprague Dawley rats were injected with CFA in the mid-region of the masseter muscle. Muscle tissue surrounding the injection site was extracted 6 h, 1, 3 and 7 days after the injection to measure TNF-α, IL-1β, IL-6 and IL-4 levels with Luminex multi-analyte profiling (xMAP) technology. The cytokine levels were compared to those obtained from naïve rats. CFA injection into the masseter muscle led to a significant time effect in the level of TNF-α compared to that of naïve rats. The pattern of changes in TNF-α level after CFA injection was significantly different between the male and female rats owing to the differences in basal levels. CFA injection induced significant time-dependent increases in the levels of IL-1β and IL-6 in the masseter muscle in both male and female rats. The level of IL-4 was slightly, but significantly, reduced in both sexes at 6 h and 3 days after CFA-induced inflammation. No significant sex differences were observed in the levels of IL-1β, IL-6 or IL-4. The results provided novel information about distinct cytokine profiles during CFA-induced muscle inflammation, and the basis for further pursuing contributions of each cytokine in pain processing and analgesic responses in both sexes.     

Inter-individual differences in cytokine release in patients undergoing cardiac surgery with cardiopulmonary bypass

Cardiac surgery with cardiopulmonary bypass (CPB) leads to a systemic inflammatory response with secretion of cytokines (e.g. IL-6, TNF-alpha, IL-1 beta and sIL-2R). The objective of the following study was to investigate in vitro and in vivo cytokine responses and white blood cell counts (WBC) of patients with high versus low cytokine secretion after a coronary artery bypass grafting (CABG) procedure. Twenty male patients undergoing elective CABG surgery with CPB under general anaesthesia were enrolled in the study. On the day of surgery (postoperatively), serum levels of TNF-alpha and IL-1 beta were significantly higher in patients of the high IL-6 level group compared to the respective values in the patient group with low IL-6 levels. The inter-individual differences in IL-6 release in patients undergoing CABG surgery with CPB were accompanied by differences in the release of other cytokines, such as TNF-alpha, IL-1 beta and sIL-2R. To understand whether genetic background plays a role in influencing cytokine plasma levels under surgical stress, we examined the distribution of polymorphic elements within the promoter regions of the TNF-alpha and IL-6 genes, and determined their genotype regarding the BAT2 gene and TNF-beta intron polymorphisms. Our preliminary data suggests that regulatory polymorphisms in or near the TNF locus, more precisely the allele set 140/150 of the BAT2 microsatellite marker combined with the G allele at -308 of the TNF-alpha gene, could be one of the genetic constructions providing for a less sensitive response to various stimuli. Our results suggest: (1) close relationships between cytokine release in the postoperative period, and (2) inter-individually varying patterns of cytokine release in patients undergoing CABG surgery with CPB.     

乌司他丁对膝关节置换术后关节炎症及周围肿胀的影响

目的 探讨乌司他丁对全膝关节置换术后膝关节炎症及周围肿胀的影响。方法 将择期行全膝关节置换手术的60例患者随机分为W组和C组,每组30例。W组于术中切皮前、术后第12 h分别静脉注射乌司他丁0.5万U/kg;C组给予等量的生理盐水,两组患者均采用相同的围手术期镇痛方案。记录两组患者术后关节腔引流量,关节周围肿胀程度及下肢深静脉血栓发生率;比较两组患者术后12 h、24 h关节腔引流液中白细胞介素1β（IL-1β）、白细胞介素6（IL-6）、肿瘤坏死因子-α（TNF-α）水平。结果 两组患者术后关节腔     

牙周炎对慢性细菌性前列腺炎大鼠前列腺的影响

目的研究牙周炎对慢性细菌性前列腺炎大鼠前列腺的影响。方法 80只SD大鼠随机分为4周组及8周组,每组40只,再将4周组和8周组分别随机分为空白对照组(N组)、慢性细菌性前列腺炎组(CBP组)、牙周炎组(PE组)和慢性细菌性前列腺炎合并牙周炎组(CBP+PE组),每组各10只。观察比较大鼠前列腺组织病理形态学改变、炎症评分、TNF-α、IL-1β的含量及牙周各项指标。结果在4周组中,PE组、CBP+PE组牙周各项指标高于N组、CBP组(P0.05),而PE组与CBP+PE组之间以及N组与CBP组之间牙周各项指标无明显差异(P0.05);CBP+PE组和CBP组前列腺组织病理、炎症评分、TNF-α、IL-1β比N组和PE组均高(P0.05),而N组与PE组之间以及CBP+PE组与CBP组之间前列腺组织病理、炎症评分、TNF-α、IL-1β无明显差异(P0.05)。在8周组中PE组、CBP+PE组牙周各项指标高于N组和CBP组(P0.05),而PE组与CBP+PE组之间以及N组与CBP组之间牙周各项指标无明显差异(P0.05);CBP+PE组、CBP组前列腺组织病理、炎症评分、TNF-α、IL-1β较N组、PE组高(P0.05),而N组与PE组之间前列腺组织病理、炎症评分、TNF-α、IL-1β无明显差异(P0.05),CBP+PE组前列腺组织病理、炎症评分、TNF-α、IL-1β则较CBP组高(P0.05)。4周组和8周组比较,N组各项观察指标无明显差异;8周PE组牙周各项指标高于4周PE组(P0.05),前列腺组织病理、炎症评分、TNF-α、IL-1β无明显差异(P0.05);8周CBP组前列腺组织病理、炎症评分、TNF-α、IL-1β较4周CBP组降低(P0.05),而牙周各项指标无明显差异(P0.05);8周CBP+PE组与4周CBP+PE组比较前列腺组织病理、炎症评分、TNF-α、IL-1β较低(P0.05),但8周CBP+PE组牙周各项指标较4周组高(P0.05)。结论慢性细菌性前列腺炎较长时间合并牙周炎,在大鼠模型上能抑制大鼠慢性细菌性前列腺炎的自愈倾向使其保持在慢性炎症阶段。     

Anti-apoptotic and anti-inflammatory effects of Silybum marianum in treatment of experimental steatohepatitis

In this study, we were aimed to evaluate the probable effect of the crud extract of Silybum marianum, with high polyphenolic content, on experimental nonalcoholic steatohepatitis (NASH). To induce NASH, a methionine and choline deficient (MCD) diet was given to N-Mary rats for 8 weeks. After NASH development, MCD-fed rats were divided into two groups: MCD groups received MCD diet and MCD + S group was fed MCD diet plus crude extract of S. marianum orally for 3 weeks. Control group was fed a normal diet for 11 weeks. Finally, all rats were sacrificed. Plasma alanine amino transferase (ALT) and aspartate amino transferase (AST) levels were evaluated. In addition, the following hepatic factors were also evaluated: liver histology, malondialdehyde (MDA) and reduced glutathione (GSH) contents, gene expressions of TNF-α and TGF-β and immunoblot evaluations of caspase-3, ERK/p-ERK, JNK/pJNK and p38/pp38. Histopathological evaluations of the liver samples revealed that treatment with the S. marianum extract has abated the severity of NASH among the MCD-fed rats. Also, a significant reduction was observed in the sera ALT and AST activities. In addition, the extract caused dramatic reduction in the elevated hepatic TNF-α and TGF-β mRNA and MDA levels along with an increase in the GSH content. Moreover, the plant extract treatments significantly lowered activation of procaspase-3 to active caspase-3 and also lowered the phosphorylated form of JNK among the same group of rats. These results suggest that the S. marianum crude extract beneficial effects on NASH are mainly due to its antioxidant and anti-inflammatory activities.     

嘌呤P2Y受体激动剂ADPβS对脊髓来源小胶质细胞产生IL-1β、IL-6和TNF-α的刺激作用

目的:观察嘌呤P2Y受体激动剂ADPβS对脊髓背角来源小胶质细胞炎症因子IL-1β、IL-6和TNF-α表达和释放的影响。方法:培养新生SD大鼠脊髓背角小胶质细胞,PCR检测ADPβS作用下小胶质细胞IL-1β、IL-6和TNF-α在mRNA水平表达变化;ELISA检测ADPβS作用下小胶质细胞IL-1β、IL-6和TNF-α释放变化。结果:与正常对照组相比,ADPβS(10μmol/L)可以刺激脊髓背角小胶质细胞Iba-1、IL-1β、IL-6和TNF-αmRNA表达上调。P2Y_(12)受体拮抗剂MRS2395和P2Y_(13)受体拮抗剂MRS2211部分阻断ADPβS刺激小胶质细胞IL-1β、IL-6和TNF-αmRNA表达上调,MRS2395和MRS2211联合预处理几乎全部阻断ADPβS刺激小胶质细胞Iba-1、IL-1β、IL-6和TNF-αmRNA表达上调。结论:P2Y_(12)/P2Y_(13)受体激活可以促进脊髓背角小胶质细胞激活和IL-1β、IL-6和TNF-αmRNA表达上调及释放。     

人巨细胞病毒IE1蛋白促进巨噬细胞分泌IL-1β、TNF-α及凋亡

为了研究HCMV IE1-GFP融合蛋白瞬时高表达对巨噬细胞分泌活性及其凋亡的影响,将真核表达载体pEGFP/IE1转染至巨噬细胞(Mφ),转染48h后,用荧光显微镜观察GFP-IE1融合蛋白或GFP的表达与定位。ELISA法检测细胞培养上清中IL-1β或TNF-α的含量以及用RT-PCR检测其mRNA的表达情况,并收集Mφ经PI染色后流式细胞术(FCM)检测其凋亡率。结果发现,GFP-IE1融合蛋白在Mφ内瞬时表达且定位于细胞核,GFP定位于整个细胞中。GFP-IE1融合蛋白在Mφ内瞬时高表达使Mφ分泌IL-1β和TNF-α量及其mRNA表达量均增加且Mφ凋亡率明显上升,与对照组比较差异有统计学意义(P<0.01),而GFP表达对Mφ分泌及其凋亡无影响(P>0.05)。HCMV IE1瞬时高表达可上调Mφ分泌细胞因子IL-1β和TNF-α并促进Mφ凋亡。     

CPAF,HSP60 and MOMP antigens elicit pro-inflammatory cytokines production in the peripheral blood mononuclear cells from genital Chlamydia trachomatis-infected patients

Background

Persistent inflammation caused by Chlamydia trachomatis in the female genital compartment represents one of the major causes of pelvic inflammatory disease (PID), ectopic pregnancy and infertility in females. Here, we examined the pro-inflammatory cytokine response following stimulation with three different types of C. trachomatis antigens, viz. chlamydial protease-like factor (CPAF), heat shock protein 60 (HSP60) and major outer membrane protein (MOMP).