丙烯腈对男工性激素水平的影响

目的探讨丙烯腈(ACN)对男工性激素水平的影响,全面评价ACN生殖危害,为保护工人及子代健康提供科学依据。方法选112名职业性接触ACN的男工为接触组,同时选择地理位置和经济条件相近的128名不接触ACN男工为对照组,用放射免疫分析法(RIA)测定其血清中睾酮(T)、黄体生成素(FSH)、卵泡刺激素(LH)和雌二醇(E2)浓度。结果接触组男工血清中T和FSH浓度明显低于对照组(P<0.05),LH和E2浓度两组之间差异无显著性。当按车间分组时,ACN浓度较低的溶剂车间的男工血清中T浓度高于其他3个车间,但差异无显著性。当接触组按个人累计外接触剂量(包括吸烟)分组时,接触组男工血清中FSH和T浓度随接触剂量增加呈下降趋势。当外接触剂量≥50g时,T浓度明显降低(P<0.05),E2浓度明显升高(P<0.05);当外接触累计剂量≥100g时,血清中FSH浓度也明显低于对照组(P<0.05)。结论ACN及其代谢产物(CEO)能诱导男工血清中FSH、T和E2水平发生改变;提示,接触ACN可能对内分泌功能和睾丸中支持细胞和间质细胞有损伤,从而可能引起性功能障碍。     

丙烯腈对男工性激素水平影响的研究

目的 探讨丙烯腈（AN）对作业男工性激素水平的影响。方法 选择71名长期接触AN的男工为接触组,选与其生活环境、经济条件、年龄相近的不接触任何毒物的男工50人为对照组,测定其血清中T、LH、FSH和E2水平。结果 接触组血清中T水平明显下降而E2明显升高,与对照组比较差异均有细胞受损,因而使男工血中T下降、E2升高,其确切机制尚需深入研究。     

锰对雄性小鼠血清性激素水平的影响

目的 探讨锰暴露对雄性小鼠血清性激素分泌的影响。 方法 选用健康雄性昆明小鼠48只,随机分为对照组和低、中、高染锰组。各组小鼠分别腹腔注射生理盐水、12.5、25.0、50.0 mg/kg MnCl₂,注射容量5 ml/kg,每天染毒1次,持续14 d,最后一天染毒24 h后处死小鼠,测量体重、睾丸和附睾的重量,腹主动脉采血,离心后取血清用酶联免疫试剂盒检测促性腺激素释放激素(gonadotropin-releasing hormone,GnRH)、卵泡刺激素(folide-stimulating hormone,FSH)、黄体生成素(luteinizing hormone,LH)和睾酮(testosterone,T)的水平。HE染色观察下丘脑神经元细胞组织形态的改变。 结果 与对照组相比,各染锰组小鼠体重、睾丸和附睾脏器系数差异无统计学意义(P>0.05),而高锰组血清中GnRH、FSH和LH含量显著升高至68.22 ng/L、11.43 U/L、2 055.82 pg/ml(P<0.05),T水平明显降低至81.25 nmol/L(P<0.05)。HE染色发现对照组可见结构清楚,排列规整,核膜清晰,染锰组逐渐形态紊乱,胞体皱缩,细胞周围间隙增大。 结论 锰暴露可引起雄性小鼠血清性激素分泌异常以及下丘脑组织形态受损,GnRH、FSH、LH明显升高,睾酮降低。     

血清FSH、LH、TSTO水平变化对多囊卵巢综合征不孕患者的临床价值

目的探讨血清卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(TSTO)水平变化对多囊卵巢综合征不孕患者的临床价值。方法选取2015年1月到2016年6月期间我院收治的多囊卵巢综合征不孕患者60例作为观察组,另外选取40例具有正常生育能力的女性体检者作为对照组,采用化学发光法检测两组对象的血清FSH、LH、TSTO水平。结果两组的血清FSH水平比较,差异无统计学意义(P>0.05)。观察组的血清LH、TSTO水平均显著高于对照组,差异具有统计学意义(P<0.05)。观察组在接受IVF-ET治疗后,IVF-ET成功率为63.3%,失败率为36.7%。IVF-ET成功患者的血清FSH水平与IVF-ET失败患者比较,差异无统计学意义(P>0.05);IVF-ET成功患者的血清LH、TSTO水平均显著低于IVF-ET失败患者,差异具有统计学意义(P<0.05)。结论多囊卵巢综合征不孕患者的FSH水平偏低或正常,血清中多见LH、TSTO的升高,尤其以LH升高较明显,多囊卵巢综合征不孕患者的IVF-ET成功率与血清LH、TSTO水平是否接近正常水平呈正相关。     

丙烯腈对雌性大鼠生殖内分泌影响的研究

目的 研究丙烯腈对雄性大鼠血清中雌二醇（E2）、卵泡刺激素（FSH）及黄体生成素（LH）水平及卵巢、子宫、肾上腺病理改变的影响。方法 采用放射免疫法测定丙烯腈经皮下注射染毒30天雄性大鼠血表中上述各激素的水平;光镜观察卵巢、子宫和肾上腺的病理改变。结果 各染毒组与对照组比较三种激素水平差异均无显著性（P＞0．05）;但卵巢及肾上腺有不同程度的病理改变。结论 在此条件下,丙烯腈对雄性大鼠的内分泌激素无影响,但对生殖系统有直接损害作用。     

大豆异黄酮对老龄雌性大鼠血清性激素水平影响

目的 观察大豆异黄酮对老龄雌性大鼠卵巢及血清中主要性激素水平影响。方法 选用20月龄老龄雌性大鼠50只, 按体重随机分为5组, 每组10只, 分别为空白对照组, 大豆异黄酮低、中、高剂量组(100、200、300 mg/kg)、雌激素组(己烯雌酚0.6 mg/kg), 连续给予8周。采用酶联免疫吸附实验测定各组大鼠卵巢及血清中雌二醇、孕酮、睾酮、黄体生成素(LH)、促卵泡生成素(FSH)、性激素结合球蛋白(SHBG)水平。结果 与对照组比较, 高剂量大豆异黄酮组大鼠卵巢/体比(0.031±0.008)明显降低(P<0.05), 血清中雌二醇含量[(5.177±0.453)μg/L]明显升高, LH含量[(3.225±0.405)ng/L]、FSH含量[(1.536±0.186)IU/L]降低(P<0.05);与对照组比较, 大豆异黄酮组大鼠血清中孕酮、睾酮、SHBG水平无明显变化(P>0.05)。结论 大豆异黄酮可通过调节老龄雌性大鼠血清中雌二醇、LH、FSH水平缓解卵巢功能衰退。     

应用男性性功能康复治疗仪治疗儿童阴茎发育不良的效果及其对患儿性激素水平的影响

目的 探讨男性性功能康复治疗仪对儿童阴茎发育不良患儿血清睾酮（T）、促卵泡刺激素（FSH）、促黄体生成素（LH）水平的影响,为临床治疗该病提供参考依据。方法 选取2021年1月至12月牡丹江市妇幼保健院收治的51例阴茎发育不良患儿作为研究对象,按照随机数字表法分为对照组（25例）和观察组（26例）。对照组患儿采用重组人绒促性素+电磁波谱治疗仪治疗,观察组患儿在对照组的基础上联合使用男性性功能康复治疗仪治疗,两组患儿均治疗20 d,并随访3个月。比较两组患儿治疗前后阴茎松弛长度、阴茎直径、阴茎牵伸长度、睾丸横纵径及血清T、FSH、LH水平,以及治疗前与治疗后3个月儿童少年生活质量量表（QLSCA）评分。结果 与治疗前比,治疗后两组患儿阴茎松弛长度、阴茎直径、阴茎牵伸长度、睾丸横纵径均增长,血清T、FSH、LH水平及治疗后3个月QLSCA分值均升高,且观察组高于对照组（均P<0.05）。结论 男性性功能康复治疗仪可调节阴茎发育不良患儿性激素水平,促进阴茎的生长发育,提高患儿生活质量。     

极低频电磁场对雄性成年大鼠生殖激素影响的实验研究

目的研究ELF EMFs对雄性生殖系统的毒性作用,系统观察ELF EMFs对暴露在不同参数及不同接触时间的雄性大鼠血清生殖激素的影响。方法健康雄性成年大鼠100只,随机分为对照组和实验组。将实验组大鼠按设计要求暴露在电磁场中,一定时间后,取对照组和实验组大鼠的血清,采用放射免疫分析(RIA)方法进行睾酮(T)、雌二醇(E_2)、卵泡刺激素(FSH)、黄体生成素(LH)等生殖激素进行检测。结果随着大鼠暴露ELF EMFs参数的加大,以及接触时间的延长,血清T、E2、FSH、LH浓度均呈现下降的趋势。结论ELF EMFs暴露对雄性大鼠血清T、E_2、FSH、LH等生殖激素的浓度具有影响,结果导致血清T、E_2、FSH、LH浓度呈现不同程度的下降,最终影响雄性大鼠的生殖能力。     

三硝基甲苯的雄性生殖毒性研究

从小鼠睾丸精母细胞染色体畸变，大鼠睾丸损伤的病理组织学以及血清睾酮（Ｔ）、卵泡刺激素（ＦＳＨ）和黄体生成素（ＬＨ）水平几个方面研究三硝基甲苯（ＴＮＴ）对雄性生殖系统的毒性。发现：ＴＮＴ可引起小鼠睾丸精母细胞染色体的畸变率较高，以染色体的断裂为主，可使大鼠睾丸组织严重损伤直至萎缩。高剂量组动物血清睾酮水平下降，其它染毒组血清ＦＳＨ、ＬＨ水平与对照组比较无差异，上述变化提示，ＴＮＴ对睾丸的损伤可能是毒物的直接作用。     

Impact of cadmium exposure on male sex hormones: a population-based study in China

The objective of this study was to investigate the possible effects of environmental cadmium (Cd) exposure on the levels of serum sex hormones in a Chinese population group. A total of 263 male volunteers were included. Blood samples were collected for the determination of serum testosterone (T), measured by radioimmunoassay, and follicle stimulating hormone (FSH) and luteinizing hormone (LH), both measured by enzyme immunoassays. Urinary and blood Cd were analyzed by atomic absorption spectroscopy (AAS). We found a dose-response relationship between urinary Cd excretion and the prevalence of abnormally high serum T levels, but, through multiple regression analysis, we could not trace exposure to Cd as a significant determinant of serum T levels. Exposure to Cd also failed to influence the levels of FSH and LH in serum. In contrast, we found that age, body mass index (BMI), and smoking habits are significant determinants of FSH and LH and of T and LH, respectively. We conclude that oral Cd exposure is not a critical determinant of hormone homeostasis in males, but lifestyle and some biological factors, such as age and BMI, are important. The relationship found between urinary Cd and high T levels may be of importance for male reproductive morbidity and should be investigated further.     

增产菊胺酯对雄性小鼠生殖激素的影响

为了探讨增产菊胺酯引起小鼠精了生成障碍的可能机理，本文研究了增产菊胺酯对雄性小鼠卵泡刺激素（ＦＳＨ）、黄体生成素（ＬＨ）及睾酮（Ｔ）的影响。小鼠经口染毒，隔天一次，连续１０次，第３５天处死。检验因清ＦＳＨ、ＬＨ没有明显变化，而睾丸组织睾酮含量出现剂理依赖性降低，高剂量组与对照组比较有显著性差异（Ｐ〈０．０５），结果表明，增产菊胺酯不影响ＦＳＨ、ＬＨ，而影响Ｔ的合成和分泌。这可能是增产菊胺酯对小鼠精     

Ethanol and the pulsatile release of luteinizing hormone, follicle stimulating hormone and prolactin in ovariectomized rats

Conscious ovariectomized rats were administered either saline or an ethanol (ETOH)-saline solution via a permanent intragastric cannula, and plasma LH, FSH and PRL were measured by RIA of jugular blood samples drawn every 10 min through an indwelling silastic catheter. Control injections of saline into the gastric cannula did not modify any of the plasma hormone concentrations. Animals which were administered ETOH, showed marked decreases in the plasma concentrations of LH. Compared to basal levels, a significant decrease in the area under the secretion curve of LH occurred during the initial hour after ETOH administration. This decline continued with the lowest levels of plasma LH being detected at approximately 1.5 hours following the ETOH injection. Additionally, no LH pulses were detected in any of the ETOH-treated animals during the second hour after ETOH; thus, reducing the number of LH pulses observed in ETOH vs. saline-injected animals. Comparable increases in the area under the LH curve occurred following a challenge dose of LHRH in both saline and ETOH-injected rats, indicating that pituitary responsiveness was the same for both groups. In contrast to LH, ETOH did not significantly alter the pattern of FSH secretion, as represented by the area under the curve and the number of FSH pulses. In addition to the differential effects of ETOH on the pulsatile release of LH and FSH, the present data also indicate that these two gonadotropins have different secretory patterns. With regard to PRL, ETOH-injected animals showed a significant elevation in plasma PRL levels during the first hour following ETOH administration.(ABSTRACT TRUNCATED AT 250 WORDS)     

Differential effects of ethanol on luteinizing hormone, follicle stimulating hormone and prolactin secretion in the female rat

Serum luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin (Prl) levels were determined in ovariectomized rats following short-term (3 day) ethanol (ETOH) administration. ETOH was given either as an ETOH-saline solution, or via a liquid diet regimen (Bio-Serve, Inc.). Rats receiving the ETOH-saline solution (3.0 g ETOH/kg) were injected via a permanent gastric cannula every 8 hr for 3 consecutive days, while control animals received injections of saline only. Each animal receiving the liquid diet regimen was provided with 40 ml of the ETOH or the isocaloric control diet ad lib during the lights-off period, followed by 40 ml of the respective diet via the gastric cannula (4 injections of 10 ml each) equally divided over the lights-on period. Additional control animals were cannulated and maintained on Lab Chow and water, but were left untreated. Both groups of ETOH-treated rats had significantly lower serum LH levels with significantly higher Prl levels when compared to values in their respective control animals. By contrast, ETOH failed to alter FSH levels. These data indicate that ETOH can differentially affect LH, FSH, and Prl secretion following short-term ETOH exposure. The dissociation observed between LH and FSH secretion following ETOH supports the hypothesis that there are separate hypothalamic control mechanisms for LH and FSH secretion.     

丙烯腈对雄性大鼠生殖内分泌系统影响的研究

目的探讨丙烯腈（ＡＮ）对雄性生殖内分泌系统的影响,为保护男工健康提供科学依据。方法应用ＲＩＡ测定大鼠血清和睾丸匀浆中Ｔ、ＬＨ、ＦＳＨ和Ｅ２水平,并对睾丸、附睾做光、电镜组织病理学检查。结果皮下注射染毒ＡＮ３８天各组大鼠各检测指标均未发现有意义的改变;染毒７７天２５ｍｇ·ｋｇ－１组血清中Ｔ下降,ＬＨ升高,睾丸中Ｔ含量下降,ＬＨ、ＦＳＨ、Ｅ２均升高;光、电镜可见间质细胞及生精上皮受到不同程度的损害。结论丙烯腈可对雄性生殖内分泌系统产生损害作用,影响血清及睾丸匀浆中性激素水平,可能危害工人及子代健康,应引起高度重视。     

亚慢性染毒2,3,7,8-四氯二苯并二噁英对雄性大鼠生殖系统的影响

目的了解2,3,7,8-四氯二苯并二噁英(TCDD)对雄性大鼠生殖系统的影响。方法清洁级Wistar雄性大鼠32只,体重(100±10)g,随机分成4组,每组8只,连续经口灌胃染毒90 d,剂量分别为2.5,25,250 ng/kg,对照组给予二甲基亚砜(DMSO),测定血清中睾酮(T)、促黄体生成素(LH)、促卵泡生成素(FSH)的激素水平以及睾丸、前列腺、精囊腺的脏器质量系数,附睾尾精子畸形率。结果与对照组比较,各染毒组T水平下降,差异有显著性(P<0.05);各染毒组FSH和LH水平上升,但差异无显著性(P>0.05);中、高剂量组的睾丸、精囊腺及所有染毒组的前列腺脏器质量系数均低于对照组(P<0.05);各染毒组精子畸形率随剂量的增加而上升,中、高剂量组与对照组相比差异显著(P<0.01)。结论TCDD亚慢性染毒,可影响精子和生殖系统的正常发育,并在一定程度上对生殖激素的稳态造成了干扰。     

The relationships between blood lead levels and serum follicle stimulating hormone and luteinizing hormone in the third National Health and Nutrition Examination Survey

The relationships between blood lead levels and serum follicle stimulating hormone and luteinizing hormone were assessed in a nationally representative sample of women, 35-60 years old, from the third National Health and Nutrition Examination Survey. The blood lead levels of the women ranged from 0.7 to 31.1 microg/dl. The estimated geometric mean was 2.2 microg/dl, and the estimated arithmetic mean was 2.8 microg/dl. As the blood lead level increased across women, the concentration of serum follicle stimulating hormone increased in post-menopausal women, women who had both ovaries removed, and pre-menopausal women. The concentration of follicle stimulating hormone decreased in pre-menopausal women who were taking birth control pills. The concentration of luteinizing hormone increased as blood lead level increased in post-menopausal women and women who had both ovaries removed. The lowest concentrations of blood lead at which a relationship was detected were 1.7 microg/dl for follicle stimulating hormone and 2.8 microg/dl for luteinizing hormone. The increase in follicle stimulating hormone and luteinizing hormone in women with no ovaries indicates that lead may act at a non-ovarian site in the female reproductive system, along with a possible effect on the ovaries.     

不同类型多囊卵巢综合征患者的临床特点

目的：探讨多囊卵巢综合征（PCOS）患者内分泌紊乱的特点,为临床的个体化治疗提供依据。方法：依据2003鹿特丹诊断标准纳入PCOS患者232例,按照体质量指数（BMI,界值23 kg/m2）和黄体生成激素/卵泡刺激素比值（LH/FSH,界值2）分组。比较不同分类后患者的基础性激素水平、抗苗勒管激素、亚急性炎症反应指标及血脂代谢指标。结果：LH/FSH≥2组PCOS患者突出表现为下丘脑-垂体-卵巢轴功能紊乱,而脂代谢异常或亚急性炎症反应并不明显。BMI≥23 kg/m2组PCOS妇女具有典型肥胖所伴随的脂代谢紊乱表型,表现为高危的致心血管疾病风险倾向。结论：PCOS以BMI=23 kg/m2,LH/FSH比值2为界值区分为显著不同代谢倾向,所揭示的各类患者的内分泌代谢特点能较好地指导临床PCOS患者的个体化治疗。     

绝经后阴道出血妇女性激素水平与子宫内膜病变的关系

目的 :探讨绝经后阴道出血的妇女性激素变化及与子宫内膜病变的关系。方法 :采用酶免疫法对 6 6例绝经后阴道出血的患者性激素水平测定。结果 :绝经后阴道出血妇女血清 E2 水平远高于正常绝经后妇女血清 E2 值 ,两者存在显著性差异( P<0 .0 1)。FSH、L H与正常绝经后妇女比较明显降低 ( P<0 .0 5 )。子宫内膜增生过长和子宫内膜癌的患者雌激素水平增高者分别占 72 .7%和 6 9.6 %。结论 :绝经后阴道出血与雌激素水平增高有关 ,并且子宫内膜癌的发生率明显增高