共查询到19条相似文献,搜索用时 218 毫秒
1.
2.
3.
莪术油诱导小鼠HepA肝癌细胞凋亡及其对bcl-2蛋白表达的影响 总被引:2,自引:0,他引:2
目的研究莪术油诱导HepA肝癌细胞凋亡的生物学活性,探讨莪术油对肝癌细胞bcl-2表达水平的影响及其作用的分子机制。方法用莪术油进行小鼠肝癌体内抑制实验,运用细胞凋亡原位末端标记及免疫组化方法分析莪术油对小鼠肝癌细胞凋亡的影响。结果莪术油能有效降低小鼠肝癌细胞bcl-2的表达,诱导细胞凋亡。结论莪术油对小鼠肝癌细胞具有明显抑制作用,其主要作用机制为降低bcl-2蛋白表达、诱导肿瘤细胞凋亡。 相似文献
4.
5.
6.
目的:探析中药治疗大肠癌机理的研究进展情况。方法:搜集近5 a来关于中药治疗大肠癌机理的实验研究相关文献,按中药不同作用机制进行分析、归纳。结果:中药在临床实践及体内外实验中已证实有明确抗大肠癌活性,现代药理学证实其可能的主要机制包括:抑制细胞增殖、诱导细胞凋亡、抑制血管生成、逆转多药耐药、抑制粘附与侵袭转移、调节免疫功能等。结论:在中医理论指导下运用中药治疗大肠癌有较强的临床应用价值和独特的优势。 相似文献
7.
8.
9.
《中药材》2020,(10)
目的:研究走马胎活性组分对肝癌细胞株HepG_2增殖、凋亡、侵袭和转移的影响以明确走马胎活性组分的抗肝癌作用。方法:MTT法测定走马胎活性组分不同浓度和不同作用时间对HepG_2增殖的影响;AnnexinV/PI染色法和流式细胞仪观察其诱导HepG_2细胞的凋亡作用;细胞划痕实验、Transwell侵袭小室和迁移小室观察其对HepG_2侵袭转移的影响。结果:走马胎活性组分作用HepG_2细胞24、48、72 h的IC_(50)分别为(8.8±0.9)、(8.2±0.9)、(7.7±0.9)μg/mL。AnnexinV/PI染色法及流式细胞仪检测均提示走马胎活性组分(4、8μg/mL)作用24 h均可明显诱导HepG_2细胞凋亡。细胞划痕实验提示走马胎活性组分(4、8μg/mL)能明显抑制肝癌HepG_2细胞的运动能力。Transwell侵袭/转移小室实验结果显示,与对照组比较,走马胎活性组分(4、8μg/mL)可显著抑制HepG_2细胞穿过小室和Matrigel胶的数量。结论:走马胎活性组分可明显抑制HepG_2细胞的增殖,诱导肝癌细胞凋亡,抑制侵袭和转移,具有较强的抗肝癌作用。 相似文献
10.
11.
Berberine shows anticancer, antibacterial, antiinflammatory and antioxidant effects and may be useful in many clinical applications. The effects of berberine on articular cartilage metabolism remain unknown, so this study was performed to evaluate these effects in vitro and in vivo. For the in vitro work, rat articular chondrocytes were cultured in a monolayer and matrix metalloproteinase-1 (MMP-1), -3, -13 and tissue inhibitor of metalloproteinase (TIMP-1) expression was evaluated by real-time quantitative PCR. Nitric oxide (NO) levels were determined using the Griess reaction, and glycosaminoglycan (GAG) release was measured using the dimethylmethylene blue method. For the in vivo work, berberine was administered by intraarticular injection, and the effects on MMPs and TIMP-1 were examined at the gene and protein levels. Berberine was found to inhibit the expression of MMP-1, -3 and -13, and increased the level of TIMP-1 at the mRNA level in a dose-dependent manner. In IL-1β-induced rat articular chondrocytes, berberine decreased IL-1β-induced GAG release and NO production. Meanwhile, high-dose berberine exhibited an anticatabolic effect in an IL-1β-induced rat osteoarthritis (OA) model. These findings suggest that berberine may play a protective role in the development of OA and may be useful in the treatment of OA. 相似文献
12.
目的:观察中药提取物梓醇和小檗碱及其配伍对3T3-L1脂肪细胞增殖,葡萄糖代谢及细胞分化的影响,初步探讨药物改善胰岛素抵抗(IR)的可能机制。方法:采用四甲基偶氮唑盐(MTT)方法检测3T3-L1前脂肪细胞的增殖;以葡萄糖氧化酶法检测药物干预后培养液中葡萄糖消耗量,并以油红O染色检测细胞分化过程中胞浆脂质的堆积。结果:与对照组比较,小檗碱组使前脂肪细胞MTT值增加29%,而小檗碱加梓醇组、梓醇组均无差异;梓醇、小檗碱及其配伍组分别使成熟脂肪细胞的葡萄糖消耗增加64%、76.5%和98%;小檗碱处理脂肪细胞胞浆脂质堆积明显低于对照组,梓醇组与对照组无差异。结论:小檗碱能促进前脂肪细胞增殖,增加葡萄糖消耗,抑制脂肪细胞分化;梓醇能促进葡萄糖吸收;其作用均不依赖胰岛素的存在。直接增加葡萄糖的吸收可能是这两种药物降低血糖,改善胰岛素抵抗的机制之一。 相似文献
13.
14.
15.
Yuan Liang Chongxi Fan Xiaolong Yan Xi Lu Hua Jiang Shouyin Di Zhiqiang Ma Yingtong Feng Zhengbin Zhang Pan Feng Xiao Feng Jianyu Feng Faguang Jin 《Phytotherapy research : PTR》2019,33(1):130-148
A fundamental element of acute lung injury (ALI) is the inflammatory response, which can affect the entire respiratory system, including the respiratory tract and alveoli. Berberine has gained attention because of its anti‐inflammatory effects. Nuclear factor‐erythroid 2‐related factor 2 (Nrf2) and endoplasmic reticulum (ER) stress are involved in lung injury. Nrf2 also acts as a protein kinase‐like ER kinase (PERK) substrate in heart disease. Therefore, this study investigated the effect of berberine against lipopolysaccharide (LPS)‐induced ALI and the role of the PERK‐mediated Nrf2/HO‐1 signaling axis. Berberine promoted Nrf2 nuclear translocation and phosphorylation in vitro. After LPS stimulation, this effect was further enhanced, whereas inflammatory factor (IL‐6 and IL‐8) release and reactive oxygen species generation were significantly decreased. Berberine effectively alleviated lung injury by reducing lung edema and neutrophil infiltration. Berberine also significantly reduced histopathological inflammatory changes via inhibition of ER stress and activation of Nrf2 signaling. Thapsigargin‐induced ER stress and small interference RNA (siRNA)‐mediated Nrf2 inhibition abrogated the protective effects of berberine in vitro, whereas siRNA‐mediated suppression of ER stress and sulforaphane‐induced Nrf2 activation further improved those effects. Importantly, ER stress induction led to Nrf2 activation, whereas PERK depletion partly reduced the level of Nrf2 phosphorylation and translocation in LPS‐induced cells. Therefore, berberine inhibits LPS‐induced ALI through the PERK‐mediated Nrf2/HO‐1 signaling axis. 相似文献
16.
赵斐 《中国民族民间医药杂志》2012,(10):48-49
目的:用HPLC法测定清热化毒丸中黄芩苷的含量。方法:采用高效液相色谱法,使用Agilent ZORBAX SB—C18(250mm×4.6mm,5μm)色谱柱,以甲醇-水-磷酸(47:53:0.2)为流动相,检测波长为315nm,流速:1.0mL·min-1,柱温:40℃。结果:黄芩苷在0.1028~1.0275μg范围内呈良好的线性关系,回归方程为Y=10225920x-1083344(r2=0.9925)。平均回收率为99.61%,RSD=1.4%。结论:本法灵敏、准确、专属性强,回收率高,重现性好,可作为清热化毒丸中黄芩苷的含量测定的检测方法。 相似文献
17.
甘露 《中国实验方剂学杂志》2011,17(14):240-242
目的:观察大鼠局灶性脑缺血再灌注后缺血脑区细胞间黏附分子-1(ICAM-1)以及P-选择素(P-selectin)的表达和黄连素对两者的影响。方法:将36只大鼠随机分为3组:假手术组,模型组,给药组。给药组分别于栓塞前15 min和栓塞后2 h,再灌注时ip给黄连素30 mg.kg-1;模型组和假手术组ip等量生理盐水。采用大脑中动脉线栓法制作大脑局灶缺血再灌注模型。再灌注24 h后取脑,应用免疫组化方法检测ICAM-1和P-selectin的表达。结果:黄连素能够显著降低缺血再灌注引起的脑梗死体积以及脑组织ICAM-1,P-selectin的表达增高(P<0.05)。其中梗死体积从25%降至17%;ICAM-1阳性细胞百分率从平均65%降至35%;P-selectin从平均60%降至30%。结论:黄连素能够通过脑缺血再灌注炎性反应的免疫调节机制保护脑缺血再灌注引起的损害。 相似文献
18.
目的 探讨小檗碱增强耐药K562/DOX细胞对化疗药多柔比星的敏感性的作用。方法 四甲基偶氮唑蓝法检测小檗碱的细胞毒性及其对多柔比星抗肿瘤活性的增强作用;高内涵活细胞成像系统检测无毒剂量小檗碱作用后,多柔比星在K562/DOX细胞内的蓄积量;PI/Hoechst33342双染法检测小檗碱对多柔比星诱导的K562/DOX细胞凋亡的影响;罗丹明123蓄积实验检测小檗碱对P-糖蛋白外排功能的影响。结果 1 μmol·L-1为小檗碱的无毒剂量,在此无毒剂量下,小檗碱使多柔比星对K562/DOX细胞的IC50降低了1.5倍;1 μmol·L-1小檗碱可使多柔比星在K562/DOX细胞内的蓄积量增加,增强多柔比星诱导的K562/DOX细胞凋亡, 增加K562/DOX细胞内罗丹明123的蓄积量,从而抑制P-糖蛋白的外排功能。结论 小檗碱可通过抑制K562/DOX细胞膜上P-糖蛋白的外排功能,增加K562/DOX细胞内多柔比星浓度,促进多柔比星对耐药细胞的诱导凋亡作用,逆转K562/DOX细胞的多药耐药性。 相似文献
19.
目的:研究8-羟基二氢小檗碱和小檗碱与多药耐药基因之间的作用。方法:采用MTT比色法测定药物的细胞毒浓度,适时定量PCR测定不同多药耐药基因的表达。结果:在1~100μM浓度范围内,8-羟基二氢小檗碱和小檗碱对Caco-2细胞无毒性作用;8-羟基二氢小檗碱和小檗碱对mdr1和mrp 1的表达作用一致,在25~100μM范围内,小檗碱能明显降低mrp2的mRNA表达,而8-羟基二氢小檗碱对其无明显影响。结论:8-羟基二氢小檗碱与小檗碱对mrp2的基因表达差异较大,其对MRP2蛋白表达的作用以及与基因调控之间的作用关系还有待更进一步的研究。 相似文献