姜黄素诱导人肝癌细胞系Huh7自噬和凋亡

目的 探讨姜黄素诱导人肝癌细胞Huh7自噬和凋亡的作用,以及抑制自噬对姜黄素诱导凋亡的影响。方法 将姜黄素（5、10、20、40 μmol/L）加入人肝癌细胞系Huh7的培养液中,用CCK-8检测细胞增殖水平变化。Huh7细胞用5~40 μmol/L姜黄素培养48 h,通过蛋白质印迹法检测自噬微管相关蛋白1轻链3（LC3）-Ⅱ/LC3-Ⅰ的比值,免疫荧光法检测自噬小体的形成,评估Huh7细胞自噬水平的变化。通过流式细胞术检测Huh7细胞凋亡情况。加入5 mmol/L自噬抑制剂3-甲基嘌呤（3-MA）和20 μmol/L姜黄素培养Huh7细胞,检测Huh7细胞凋亡水平和自噬水平的变化。结果 CCK-8检测显示姜黄素能抑制Huh7细胞的增殖（P<0.05,P<0.01）,且细胞凋亡率随着姜黄素浓度的升高而上升;蛋白质印迹检测显示加入姜黄素后LC3-Ⅱ/LC3-Ⅰ的比值上升（P<0.05,P<0.01）,免疫荧光检测显示加入20 μmol/L姜黄素后自噬小体增多。与单独使用20 μmol/L姜黄素培养的Huh7细胞相比,姜黄素联用3-MA培养的Huh7细胞LC3-Ⅱ/LC3-Ⅰ的比值下降（P<0.01）,自噬小体减少。流式细胞术检测发现5~40 μmol/L姜黄素促进了Huh7细胞凋亡（P<0.05,P<0.01）,而联用3-MA后与单独使用20 μmol/L姜黄素培养相比引起的Huh7细胞凋亡减少（P<0.05）。结论 姜黄素能诱导Huh7细胞凋亡、抑制细胞增殖并促进细胞自噬,抑制自噬能减弱姜黄素对Huh7细胞的诱导凋亡效应。     

尼古丁对血管内皮细胞自噬的影响

目的 初步探讨尼古丁对人脐静脉内皮细胞（human umbilical vein endotheliel cell,HUVEC）自噬的影响。方法 采用蛋白免疫印迹（Western blot）技术分别检测尼古丁不同浓度（0、6×10^-8、6×10^-7、6×10^-6和6×10^-5mol/L）和不同时间（浓度为6×10^-6mol/L时,分别干预0、1.5、3、6和12h）作用后,人脐静脉内皮细胞中自噬标志性蛋白微管相关蛋白轻链3Ⅱ（LC3Ⅱ）蛋白表达水平的变化,以确定尼古丁的最佳刺激浓度和最佳刺激时间。依据尼古丁最佳条件将体外培养的HUVE细胞随机分组：对照组、尼古丁处理组,Western blot法检测LC3Ⅱ、多聚泛素结合蛋白p62/SQSTM1（p62）和溶酶体相关膜蛋白-2（LAMP-2）的蛋白表达,Annexin Ⅴ-FITC/PI双染流式细胞术（flow cytometry,FCM）检测细胞凋亡率。结果 体外实验显示,LC3Ⅱ蛋白表达呈浓度和时间依赖性,且尼古丁浓度为6×10^-6mol/L、时间为6h时,LC3Ⅱ蛋白表达最多（P<0.01）。与对照组相比,尼古丁处理组LC3Ⅱ、p62蛋白表达显著增加（P<0.05）,LAMP2蛋白表达减少（P<0.05）,细胞凋亡率升高（P<0.01）。结论 尼古丁可以引起自噬体与溶酶体融合障碍,使自噬降解异常,导致内皮细胞自噬功能紊乱,促进细胞凋亡。     

TLR2/NF⁃κB通路介导MPP+诱导的RSC96细胞凋亡与自噬

目的：探讨1-甲基-4-苯基-吡啶离子（1-methyl-4-phenyl-pyridinium,MPP⁺ ）诱导的RSC96细胞凋亡与自噬功能障碍与TLR2/NF-κB信号通路的关系。方法：将RSC96细胞分为PBS组、MPP⁺ 组和MPP⁺ +CU-CPT22组;CCK-8检测不同MPP⁺ 浓度 （0.1、0.3、0.5、0.7、0.9 mmol/L）处理后细胞存活率;TUNEL检测细胞凋亡水平;RT-qPCR检测Toll样受体2（TLR2）mRNA转录水平;Western blot检测凋亡相关指标Bcl-2/Bax、cleaved caspase-3/caspase-3,自噬相关指标LC3Ⅱ/LC3Ⅰ和P62,以及TLR2、p-NF- κB/NF-κB蛋白表达水平。结果：与PBS组相比,MPP⁺ 组细胞存活率下降且呈浓度依赖性,TUNEL染色阳性细胞数增多,Bcl-2/ Bax蛋白比值水平下降,cleaved caspase-3/caspase-3比值增高。同时LC3Ⅱ/LC3Ⅰ比值下降,P62表达增加,p-NF-κB/NF-κB比值表达增加。RT-qPCR和Western blot结果显示,MPP⁺ 上调TLR2的表达。此外,与MPP⁺ 组相比,MPP⁺ +CU-CPT22组TUNEL阳性细胞数目减少,Bcl-2/Bax比值升高,cleaved caspase-3/caspase-3比值降低;同时,LC3Ⅱ/LC3Ⅰ比值上升,P62水平下降。结论： MPP⁺ 刺激诱导RSC96细胞凋亡和自噬水平失衡,发生机制可能与TLR2/NF-κB通路的激活有关。     

低糖培养对H9c2心肌细胞的自噬功能和细胞凋亡的影响

目的 研究低糖培养对H9c2心肌细胞自噬活性的影响及此时自噬功能对细胞凋亡水平的影响。方法 用33.3mmol/L葡萄糖浓度的培养基培养H9c2细胞后用2.5mmol/L葡萄糖浓度培养基处理细胞4h作为低糖组（LG组）,高糖组（HG组）维持33.3mmol/L的葡萄糖浓度,对照组（Con组）维持5.5mmol/L的葡萄糖浓度;LG组加入自噬抑制剂3-MA作为抑制剂组（LG+3-MA组）。采用LDH检测试剂盒检测细胞毒性;MTT法检测H9c2细胞增殖能力; Western blot法检测caspase3、LC3、Beclin-1的表达量。结果 与Con组和HG组相比,LG组LDH活性显著增加,细胞增殖能力显著降低,caspase3、LC3-Ⅱ和Beclin-1表达显著增加;而与LG组比较,抑制剂组LC3-Ⅱ、Beclin-1表达降低,同时caspase3以及细胞毒性降低,细胞活力增加。结论 低糖培养通过激活H9c2心肌细胞的自噬活性引起细胞凋亡。     

姜黄素联合雷帕霉素诱导去势难治性前列腺癌Pten-CaP8细胞自噬及凋亡研究

目的 探讨姜黄素联合雷帕霉素对去势难治性前列腺癌（CRPC）Pten-CaP8细胞增殖、自噬及凋亡的影响。方法 将不同浓度的姜黄素单独或与雷帕霉素共同处理Pten-CaP8细胞24 h后,MTT法检测细胞增殖,光镜下观察细胞形态,Western blotting法检测相关蛋白表达。结果 姜黄素联合雷帕霉素可显著抑制Pten-CaP8细胞增殖（P＜0.05）,上调LC3-II/LC3-I表达,诱导PARP裂解,还可下调p-AKT（S473）、p-S6（S240/244）、AR（N-20）和Cyclin D1蛋白表达水平,使Pten-CaP8细胞形态变短且胞浆内出现空泡改变。结论 姜黄素联合雷帕霉素可共同诱导Pten-CaP8细胞自噬及凋亡且效果显著,两者协同抗癌机制与其拮抗PI3K/Akt/mTOR信号通路相关。     

血红素加氧酶-1的表达对氟尿嘧啶诱导食管癌细胞凋亡的影响

目的 本研究探讨血红素加氧酶-1与氟尿嘧啶诱导食管癌细胞凋亡的关系。 方法 培养ECa109食管鳞癌细胞,实验组分别加入正常培养基,含20μmol/L、80μmol/L锌原卟啉(ZnppIX)的培养基培养12h,后更换为含100μg/ml 5-FU组培养;对照组予正常培养基培养;共培养96h后检测各组细胞凋亡情况。 结果 在凋亡早期细胞中(Annexin V⁺/PI^-),20μmol/L ZnppIX组(25.63%±6.52%)及80μmol/L ZnppIX(22.48%±5.59%)组显著高于5-FU组(12.89%±4.42%,P均<0.05)。在中晚期凋亡细胞(Annexin V⁺/PI⁺)中,20μmol/L ZnppIX组(25.17%±5.53%)显著低于5-FU组(39.89%±6.66%)及80μmol/L ZnppIX组(41.95%±2.92%,P均<0.01)。80μmol/L ZnppIX组caspase-3的活化率显著高于正常细胞(P<0.05)。 结论 ZnppIX抑制HO-1表达后,可以显著增加5-FU诱导的Eca109食管癌细胞凋亡率,caspase-3参与了其诱导细胞凋亡的过程。     

雷帕霉素对高糖诱导的肾系膜细胞自噬抑制、氧化损伤和衰老的影响

目的探讨雷帕霉素及3-甲基腺嘌呤(3-MA)对高糖诱导的原代大鼠系膜细胞自噬、氧化损伤及衰老的影响。方法从大鼠肾分离培养原代肾小球系膜细胞(GMCs),分为正常对照组、高糖组、高糖+雷帕霉素(自噬增强剂)及高糖+3-甲基腺嘌呤(自噬抑制剂)干预组。在细胞培养24 h、72 h及10 d时,Western blotting观测自噬标志物-自噬相关基因LC3及泛素结合蛋白p62/SQSTMI的表达变化;硫代巴比妥酸法检测细胞脂质损伤产物-丙二醛(MDA)水平,2,4-二硝基苯肼(DNPH)比色法测定细胞蛋白质损伤产物-羰基含量,通过进行衰老相关β-半乳糖苷酶活性(SA-β-gal)染色及衰老相关异染色质斑块(SAHF)分析检测细胞的衰老程度。结果与对照组相比,细胞经高糖处理72 h和10 d后,自噬标志物LC3表达降低,p62/SQSTMI升高,MDA及羰基含量上升(均P<0.05);处理72 h细胞SA-β-gal染色阳性率增加(P<0.05),细胞核异染色质斑块形成增加。高糖培养的细胞用雷帕霉素干预72 h和10 d后,LC3表达升高,p62/SQSTMI表达下降,MDA及蛋白质羰基含量均下降(均P<0.05),72 h后细胞SA-β-gal染色阳性率下降(P<0.05),细胞核异染色质斑块形成减少;高糖培养的细胞用3-MA干预72 h和10 d后,LC3表达下降,p62/SQSTMI表达升高,MDA及蛋白质羰基含量升高(均P<0.05),72 h细胞SA-β-gal染色阳性率上升(P<0.05),细胞核斑块形成增多。结论高糖可抑制系膜细胞自噬功能,促进系膜细胞氧化损伤,加速细胞衰老;雷帕霉素干预可减轻高糖对系膜细胞自噬功能抑制,减少氧化损伤,延缓衰老;3-MA加重高糖对系膜细胞自噬功能的抑制,进一步加重细胞氧化损伤,加速衰老。     

花旗松素通过PI3K/AKT/mTOR通路对宫颈癌SiHa细胞自噬、凋亡和衰老的影响

[目的] 观察花旗松素（Taxifolin）对宫颈癌SiHa细胞自噬,凋亡和衰老的影响。[方法] 采用0、5、10、20μmol/LTaxifolin处理宫颈癌SiHa细胞。CCK-8法检测细胞细胞增殖倍数;蛋白免疫印迹检测Beclin1、p62、LC3II/LC3I蛋白表达水平;免疫荧光检测LC3+含量;流式细胞仪检测细胞凋亡;流式分选检测线粒体膜电位;蛋白免疫印迹检测Bcl-2、Bax、Caspase-3、cleavedCaspase-3、Caspase-9、cleavedCaspase-9、磷脂酰肌醇-3-激酶（PI3K）、pPI3K、蛋白激酶B（AKT）、p-AKT、哺乳动物雷帕霉素靶蛋白（mTOR）、p-mTOR蛋白表达水平,加入AKT激活剂SC79进行验证。[结果] 与Taxifolin0μmol/L组相比较,Taxifolin10、20μmol/L组细胞增殖倍数显著降低（P<0.05）,p62蛋白水平显著降低（P<0.05）,Beclin1、LC3II/LC3I蛋白水平显著升高（P<0.05）,LC3+含量显著升高（P<0.05）,凋亡率显著升高（P<0.05）,线粒体膜电位显著升高（P<0.05）,Bcl-2/Bax比值显著降低（P<0.05）,cleavedCaspase-3/Caspase-3、cleavedCaspase-9/Caspase-9比值升高（P<0.05）,p-PI3K/PI3K、p-AKT/AKT、p-mTOR/mTOR比值显著降低（P<0.05）。加入PI3K/AKT信号通路激活剂SC79可逆转花旗松素对SiHa细胞凋亡、自噬及PI3K/AKT信号通路相关蛋白表达的影响。[结论] Taxifolin通过抑制PI3K/AKT/mTOR通路的活化诱导宫颈癌SiHa细胞自噬,凋亡和衰老。     

紫草素抑制PI3K/Akt/mTOR通路逆转人胃癌细胞奥沙利铂耐药

目的 探讨紫草素对人胃癌细胞奥沙利铂耐药的影响及机制。方法 以0、2.5、5.0、10.0、20.0、40.0μmol/L 紫草素干预对数生长期人胃癌奥沙利铂耐药细胞(MGC803/L-OHP)24h,CCK-8法检测细胞增殖抑制率,参照半抑制浓度(IC₅₀)设定后续实验紫草素浓度。取对数生长期MGC803/L-OHP细胞,设空白对照组、奥沙利铂组、紫草素+奥沙利铂组、紫草素+奥沙利铂+IGF-1(PI₃K激活剂)组。药物干预24h后,检测细胞增殖抑制率和凋亡率,GFP-LC3质粒转染后观察自噬小体,Western blot法检测p-PI₃K、p-Akt、p-mTOR、caspase-3、cleaved caspase-3、Beclin1、LC3表达。结果 紫草素对MGC803/L-OHP细胞增殖抑制作用呈剂量依赖性,IC₅₀值为9.58μmol/L,后续实验紫草素浓度设定为10μmol/L。与空白对照组比较,奥沙利铂组细胞增殖抑制率、凋亡率升高(P<0.05),自噬小体增多。与奥沙利铂组比较,紫草素+奥沙利铂组细胞增殖抑制率、凋亡率升高(P<0.05),自噬小体增多;p-PI₃K、p-Akt、p-mTOR表达下调,cleaved caspase-3、Beclin1表达上调,cleaved caspase-3/caspase-3、LC3-Ⅱ/ LC3-Ⅰ升高(P<0.05)。与紫草素+奥沙利铂组比较,紫草素+奥沙利铂+IGF-1组细胞增殖抑制率、凋亡率降低(P<0.05),自噬小体减少;p-PI₃K、p-Akt、p-mTOR表达上调,cleaved caspase-3、Beclin1表达下调,cleaved caspase-3/caspase-3、LC3-Ⅱ/ LC3-Ⅰ降低(P<0.05)。结论 紫草素可逆转人胃癌细胞奥沙利铂耐药,其机制可能与抑制PI₃K/Akt/mTOR通路而促进胃癌细胞凋亡和自噬有关。     

依普利酮对大鼠系膜细胞凋亡的影响

目的 探讨醛固酮受体拮抗剂依普利酮对大鼠系膜细胞凋亡的影响.方法 体外培养大鼠永生系膜细胞,分为3组:对照组(A组)、醛固酮(10-7 mol/L)组(B组)、醛固酮(10-7 mol/L)+依普利酮(10-7 mol/L)组(C组).各组细胞加药培养24 h后,应用流式细胞术及Hoechst 33258染色检测细胞凋亡.应用casepase-3活性检测试剂盒检测系膜细胞casepase-3活性.结果 与对照组相比,醛固酮组系膜细胞凋亡率明显增加,casepase-3活性明显增加(P＜0.05).与醛固酮组相比,醛固酮+依普利酮组细胞凋亡率明显降低,casepase-3活性明显降低(P＜0.05).结论 依普利酮可以通过caspase途径抑制醛固酮诱导的大鼠系膜细胞凋亡.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.