染铅小鼠海马不饱和脂肪酸含量研究

Objective To study possible impairment mechanisms of learning and memory abilities from unsaturated fatty acids in hippocampus of mice exposed to lead.Methods Forty-eight healthy mice were divided into 4 groups:low dose(0.625 g/L),middle dose(1.250 g/L) and high dose(2.500 g/L) of lead solution in diet and control group (distilled water).The mice in treatment groups were fed with lead solution every day while the mice in control group were fed with distilled water for 50 days.After learning and memory abilities were measured,the mice were killed and contents of oleic acid (C 18:1),linoleic acid (C 18:2),linolenic acid (C18:3),arachidonic acid (AA,C20:4),eicosapentaenoic acid (EPA,C20:5) and docosabexaenoie acid (DHA,C22:6) in hippocampus of mice were determined by high performance liquid chromatography (HPLC).Results (1) In the four training days,the mice treated with lead in the middle dose group and high dose group significantly increased the escape latencies compared with the mice treated with distilled water (P＜0.05) ,and on the 4th day,the low dosage mice' s escape latencies were delayed (P＜O.05).The escape latencies of the I st,2nd,3rd and 4th day had significantly positive linear relation with lead dose.Their relative coefficient in turn is r=0.973,0.985,0.929 and 0.936,indicating that lead harmed spatial memory of mice in Morris water maze (MWM).(2)The contents of C18:2 and AA were obviously enhanced in hippocampus of middle and high dosage (P＜0.05);while there was evident decrease in the contents of C 18:3,EPA and DHA (P＜0.05);the content of C 18:1 was decreased significantly in high dosage group (P＜0.01).The mice's escape latencies had significantly negative linear relation with contents of C 18:1,C 18:3,EPA and DHA,while there was positive linear relation significantly with contents of C18:2 and AA.Their relative coefficient in turn was r=-0.901,-0.914,-0.893,-0.855,0.936,0.727.Conclusion Lead interferes with the metabolism of hippocampus fatty acids and affects membrane function in hippocampus of mice,which might contribute to change of the synthesis,metabolism and release of central neurotransmitter and decrease of the learning and memory abilities.     

染铅小鼠海马不饱和脂肪酸含量研究

Objective To study possible impairment mechanisms of learning and memory abilities from unsaturated fatty acids in hippocampus of mice exposed to lead.Methods Forty-eight healthy mice were divided into 4 groups:low dose(0.625 g/L),middle dose(1.250 g/L) and high dose(2.500 g/L) of lead solution in diet and control group (distilled water).The mice in treatment groups were fed with lead solution every day while the mice in control group were fed with distilled water for 50 days.After learning and memory abilities were measured,the mice were killed and contents of oleic acid (C 18:1),linoleic acid (C 18:2),linolenic acid (C18:3),arachidonic acid (AA,C20:4),eicosapentaenoic acid (EPA,C20:5) and docosabexaenoie acid (DHA,C22:6) in hippocampus of mice were determined by high performance liquid chromatography (HPLC).Results (1) In the four training days,the mice treated with lead in the middle dose group and high dose group significantly increased the escape latencies compared with the mice treated with distilled water (P＜0.05) ,and on the 4th day,the low dosage mice' s escape latencies were delayed (P＜O.05).The escape latencies of the I st,2nd,3rd and 4th day had significantly positive linear relation with lead dose.Their relative coefficient in turn is r=0.973,0.985,0.929 and 0.936,indicating that lead harmed spatial memory of mice in Morris water maze (MWM).(2)The contents of C18:2 and AA were obviously enhanced in hippocampus of middle and high dosage (P＜0.05);while there was evident decrease in the contents of C 18:3,EPA and DHA (P＜0.05);the content of C 18:1 was decreased significantly in high dosage group (P＜0.01).The mice's escape latencies had significantly negative linear relation with contents of C 18:1,C 18:3,EPA and DHA,while there was positive linear relation significantly with contents of C18:2 and AA.Their relative coefficient in turn was r=-0.901,-0.914,-0.893,-0.855,0.936,0.727.Conclusion Lead interferes with the metabolism of hippocampus fatty acids and affects membrane function in hippocampus of mice,which might contribute to change of the synthesis,metabolism and release of central neurotransmitter and decrease of the learning and memory abilities.     

染铅小鼠海马不饱和脂肪酸含量研究

Objective To study possible impairment mechanisms of learning and memory abilities from unsaturated fatty acids in hippocampus of mice exposed to lead.Methods Forty-eight healthy mice were divided into 4 groups:low dose(0.625 g/L),middle dose(1.250 g/L) and high dose(2.500 g/L) of lead solution in diet and control group (distilled water).The mice in treatment groups were fed with lead solution every day while the mice in control group were fed with distilled water for 50 days.After learning and memory abilities were measured,the mice were killed and contents of oleic acid (C 18:1),linoleic acid (C 18:2),linolenic acid (C18:3),arachidonic acid (AA,C20:4),eicosapentaenoic acid (EPA,C20:5) and docosabexaenoie acid (DHA,C22:6) in hippocampus of mice were determined by high performance liquid chromatography (HPLC).Results (1) In the four training days,the mice treated with lead in the middle dose group and high dose group significantly increased the escape latencies compared with the mice treated with distilled water (P＜0.05) ,and on the 4th day,the low dosage mice' s escape latencies were delayed (P＜O.05).The escape latencies of the I st,2nd,3rd and 4th day had significantly positive linear relation with lead dose.Their relative coefficient in turn is r=0.973,0.985,0.929 and 0.936,indicating that lead harmed spatial memory of mice in Morris water maze (MWM).(2)The contents of C18:2 and AA were obviously enhanced in hippocampus of middle and high dosage (P＜0.05);while there was evident decrease in the contents of C 18:3,EPA and DHA (P＜0.05);the content of C 18:1 was decreased significantly in high dosage group (P＜0.01).The mice's escape latencies had significantly negative linear relation with contents of C 18:1,C 18:3,EPA and DHA,while there was positive linear relation significantly with contents of C18:2 and AA.Their relative coefficient in turn was r=-0.901,-0.914,-0.893,-0.855,0.936,0.727.Conclusion Lead interferes with the metabolism of hippocampus fatty acids and affects membrane function in hippocampus of mice,which might contribute to change of the synthesis,metabolism and release of central neurotransmitter and decrease of the learning and memory abilities.     

染铅小鼠海马不饱和脂肪酸含量研究

Objective To study possible impairment mechanisms of learning and memory abilities from unsaturated fatty acids in hippocampus of mice exposed to lead.Methods Forty-eight healthy mice were divided into 4 groups:low dose(0.625 g/L),middle dose(1.250 g/L) and high dose(2.500 g/L) of lead solution in diet and control group (distilled water).The mice in treatment groups were fed with lead solution every day while the mice in control group were fed with distilled water for 50 days.After learning and memory abilities were measured,the mice were killed and contents of oleic acid (C 18:1),linoleic acid (C 18:2),linolenic acid (C18:3),arachidonic acid (AA,C20:4),eicosapentaenoic acid (EPA,C20:5) and docosabexaenoie acid (DHA,C22:6) in hippocampus of mice were determined by high performance liquid chromatography (HPLC).Results (1) In the four training days,the mice treated with lead in the middle dose group and high dose group significantly increased the escape latencies compared with the mice treated with distilled water (P＜0.05) ,and on the 4th day,the low dosage mice' s escape latencies were delayed (P＜O.05).The escape latencies of the I st,2nd,3rd and 4th day had significantly positive linear relation with lead dose.Their relative coefficient in turn is r=0.973,0.985,0.929 and 0.936,indicating that lead harmed spatial memory of mice in Morris water maze (MWM).(2)The contents of C18:2 and AA were obviously enhanced in hippocampus of middle and high dosage (P＜0.05);while there was evident decrease in the contents of C 18:3,EPA and DHA (P＜0.05);the content of C 18:1 was decreased significantly in high dosage group (P＜0.01).The mice's escape latencies had significantly negative linear relation with contents of C 18:1,C 18:3,EPA and DHA,while there was positive linear relation significantly with contents of C18:2 and AA.Their relative coefficient in turn was r=-0.901,-0.914,-0.893,-0.855,0.936,0.727.Conclusion Lead interferes with the metabolism of hippocampus fatty acids and affects membrane function in hippocampus of mice,which might contribute to change of the synthesis,metabolism and release of central neurotransmitter and decrease of the learning and memory abilities.     

染铅小鼠海马不饱和脂肪酸含量研究

Objective To study possible impairment mechanisms of learning and memory abilities from unsaturated fatty acids in hippocampus of mice exposed to lead.Methods Forty-eight healthy mice were divided into 4 groups:low dose(0.625 g/L),middle dose(1.250 g/L) and high dose(2.500 g/L) of lead solution in diet and control group (distilled water).The mice in treatment groups were fed with lead solution every day while the mice in control group were fed with distilled water for 50 days.After learning and memory abilities were measured,the mice were killed and contents of oleic acid (C 18:1),linoleic acid (C 18:2),linolenic acid (C18:3),arachidonic acid (AA,C20:4),eicosapentaenoic acid (EPA,C20:5) and docosabexaenoie acid (DHA,C22:6) in hippocampus of mice were determined by high performance liquid chromatography (HPLC).Results (1) In the four training days,the mice treated with lead in the middle dose group and high dose group significantly increased the escape latencies compared with the mice treated with distilled water (P＜0.05) ,and on the 4th day,the low dosage mice' s escape latencies were delayed (P＜O.05).The escape latencies of the I st,2nd,3rd and 4th day had significantly positive linear relation with lead dose.Their relative coefficient in turn is r=0.973,0.985,0.929 and 0.936,indicating that lead harmed spatial memory of mice in Morris water maze (MWM).(2)The contents of C18:2 and AA were obviously enhanced in hippocampus of middle and high dosage (P＜0.05);while there was evident decrease in the contents of C 18:3,EPA and DHA (P＜0.05);the content of C 18:1 was decreased significantly in high dosage group (P＜0.01).The mice's escape latencies had significantly negative linear relation with contents of C 18:1,C 18:3,EPA and DHA,while there was positive linear relation significantly with contents of C18:2 and AA.Their relative coefficient in turn was r=-0.901,-0.914,-0.893,-0.855,0.936,0.727.Conclusion Lead interferes with the metabolism of hippocampus fatty acids and affects membrane function in hippocampus of mice,which might contribute to change of the synthesis,metabolism and release of central neurotransmitter and decrease of the learning and memory abilities.     

染铅小鼠海马不饱和脂肪酸含量研究

Objective To study possible impairment mechanisms of learning and memory abilities from unsaturated fatty acids in hippocampus of mice exposed to lead.Methods Forty-eight healthy mice were divided into 4 groups:low dose(0.625 g/L),middle dose(1.250 g/L) and high dose(2.500 g/L) of lead solution in diet and control group (distilled water).The mice in treatment groups were fed with lead solution every day while the mice in control group were fed with distilled water for 50 days.After learning and memory abilities were measured,the mice were killed and contents of oleic acid (C 18:1),linoleic acid (C 18:2),linolenic acid (C18:3),arachidonic acid (AA,C20:4),eicosapentaenoic acid (EPA,C20:5) and docosabexaenoie acid (DHA,C22:6) in hippocampus of mice were determined by high performance liquid chromatography (HPLC).Results (1) In the four training days,the mice treated with lead in the middle dose group and high dose group significantly increased the escape latencies compared with the mice treated with distilled water (P＜0.05) ,and on the 4th day,the low dosage mice' s escape latencies were delayed (P＜O.05).The escape latencies of the I st,2nd,3rd and 4th day had significantly positive linear relation with lead dose.Their relative coefficient in turn is r=0.973,0.985,0.929 and 0.936,indicating that lead harmed spatial memory of mice in Morris water maze (MWM).(2)The contents of C18:2 and AA were obviously enhanced in hippocampus of middle and high dosage (P＜0.05);while there was evident decrease in the contents of C 18:3,EPA and DHA (P＜0.05);the content of C 18:1 was decreased significantly in high dosage group (P＜0.01).The mice's escape latencies had significantly negative linear relation with contents of C 18:1,C 18:3,EPA and DHA,while there was positive linear relation significantly with contents of C18:2 and AA.Their relative coefficient in turn was r=-0.901,-0.914,-0.893,-0.855,0.936,0.727.Conclusion Lead interferes with the metabolism of hippocampus fatty acids and affects membrane function in hippocampus of mice,which might contribute to change of the synthesis,metabolism and release of central neurotransmitter and decrease of the learning and memory abilities.     

染铅小鼠海马不饱和脂肪酸含量研究

Objective To study possible impairment mechanisms of learning and memory abilities from unsaturated fatty acids in hippocampus of mice exposed to lead.Methods Forty-eight healthy mice were divided into 4 groups:low dose(0.625 g/L),middle dose(1.250 g/L) and high dose(2.500 g/L) of lead solution in diet and control group (distilled water).The mice in treatment groups were fed with lead solution every day while the mice in control group were fed with distilled water for 50 days.After learning and memory abilities were measured,the mice were killed and contents of oleic acid (C 18:1),linoleic acid (C 18:2),linolenic acid (C18:3),arachidonic acid (AA,C20:4),eicosapentaenoic acid (EPA,C20:5) and docosabexaenoie acid (DHA,C22:6) in hippocampus of mice were determined by high performance liquid chromatography (HPLC).Results (1) In the four training days,the mice treated with lead in the middle dose group and high dose group significantly increased the escape latencies compared with the mice treated with distilled water (P＜0.05) ,and on the 4th day,the low dosage mice' s escape latencies were delayed (P＜O.05).The escape latencies of the I st,2nd,3rd and 4th day had significantly positive linear relation with lead dose.Their relative coefficient in turn is r=0.973,0.985,0.929 and 0.936,indicating that lead harmed spatial memory of mice in Morris water maze (MWM).(2)The contents of C18:2 and AA were obviously enhanced in hippocampus of middle and high dosage (P＜0.05);while there was evident decrease in the contents of C 18:3,EPA and DHA (P＜0.05);the content of C 18:1 was decreased significantly in high dosage group (P＜0.01).The mice's escape latencies had significantly negative linear relation with contents of C 18:1,C 18:3,EPA and DHA,while there was positive linear relation significantly with contents of C18:2 and AA.Their relative coefficient in turn was r=-0.901,-0.914,-0.893,-0.855,0.936,0.727.Conclusion Lead interferes with the metabolism of hippocampus fatty acids and affects membrane function in hippocampus of mice,which might contribute to change of the synthesis,metabolism and release of central neurotransmitter and decrease of the learning and memory abilities.     

染铅小鼠海马不饱和脂肪酸含量研究

Objective To study possible impairment mechanisms of learning and memory abilities from unsaturated fatty acids in hippocampus of mice exposed to lead.Methods Forty-eight healthy mice were divided into 4 groups:low dose(0.625 g/L),middle dose(1.250 g/L) and high dose(2.500 g/L) of lead solution in diet and control group (distilled water).The mice in treatment groups were fed with lead solution every day while the mice in control group were fed with distilled water for 50 days.After learning and memory abilities were measured,the mice were killed and contents of oleic acid (C 18:1),linoleic acid (C 18:2),linolenic acid (C18:3),arachidonic acid (AA,C20:4),eicosapentaenoic acid (EPA,C20:5) and docosabexaenoie acid (DHA,C22:6) in hippocampus of mice were determined by high performance liquid chromatography (HPLC).Results (1) In the four training days,the mice treated with lead in the middle dose group and high dose group significantly increased the escape latencies compared with the mice treated with distilled water (P＜0.05) ,and on the 4th day,the low dosage mice' s escape latencies were delayed (P＜O.05).The escape latencies of the I st,2nd,3rd and 4th day had significantly positive linear relation with lead dose.Their relative coefficient in turn is r=0.973,0.985,0.929 and 0.936,indicating that lead harmed spatial memory of mice in Morris water maze (MWM).(2)The contents of C18:2 and AA were obviously enhanced in hippocampus of middle and high dosage (P＜0.05);while there was evident decrease in the contents of C 18:3,EPA and DHA (P＜0.05);the content of C 18:1 was decreased significantly in high dosage group (P＜0.01).The mice's escape latencies had significantly negative linear relation with contents of C 18:1,C 18:3,EPA and DHA,while there was positive linear relation significantly with contents of C18:2 and AA.Their relative coefficient in turn was r=-0.901,-0.914,-0.893,-0.855,0.936,0.727.Conclusion Lead interferes with the metabolism of hippocampus fatty acids and affects membrane function in hippocampus of mice,which might contribute to change of the synthesis,metabolism and release of central neurotransmitter and decrease of the learning and memory abilities.     

染铅小鼠海马不饱和脂肪酸含量研究

Objective To study possible impairment mechanisms of learning and memory abilities from unsaturated fatty acids in hippocampus of mice exposed to lead.Methods Forty-eight healthy mice were divided into 4 groups:low dose(0.625 g/L),middle dose(1.250 g/L) and high dose(2.500 g/L) of lead solution in diet and control group (distilled water).The mice in treatment groups were fed with lead solution every day while the mice in control group were fed with distilled water for 50 days.After learning and memory abilities were measured,the mice were killed and contents of oleic acid (C 18:1),linoleic acid (C 18:2),linolenic acid (C18:3),arachidonic acid (AA,C20:4),eicosapentaenoic acid (EPA,C20:5) and docosabexaenoie acid (DHA,C22:6) in hippocampus of mice were determined by high performance liquid chromatography (HPLC).Results (1) In the four training days,the mice treated with lead in the middle dose group and high dose group significantly increased the escape latencies compared with the mice treated with distilled water (P＜0.05) ,and on the 4th day,the low dosage mice' s escape latencies were delayed (P＜O.05).The escape latencies of the I st,2nd,3rd and 4th day had significantly positive linear relation with lead dose.Their relative coefficient in turn is r=0.973,0.985,0.929 and 0.936,indicating that lead harmed spatial memory of mice in Morris water maze (MWM).(2)The contents of C18:2 and AA were obviously enhanced in hippocampus of middle and high dosage (P＜0.05);while there was evident decrease in the contents of C 18:3,EPA and DHA (P＜0.05);the content of C 18:1 was decreased significantly in high dosage group (P＜0.01).The mice's escape latencies had significantly negative linear relation with contents of C 18:1,C 18:3,EPA and DHA,while there was positive linear relation significantly with contents of C18:2 and AA.Their relative coefficient in turn was r=-0.901,-0.914,-0.893,-0.855,0.936,0.727.Conclusion Lead interferes with the metabolism of hippocampus fatty acids and affects membrane function in hippocampus of mice,which might contribute to change of the synthesis,metabolism and release of central neurotransmitter and decrease of the learning and memory abilities.     

百草枯对发育中小鼠学习记忆能力的影响

目的 探讨百草枯对发育中小鼠学习记忆能力的影响及可能的氧化损伤机制.方法 80只健康21 日龄断奶未成熟昆明种小鼠随机分为低、中、高剂量百草枯染毒组和对照组(四蒸水),每组20只,百草枯染毒剂量分别为0.89、2.67、8.00mg/kg,经口灌胃(1次/d),连续染毒28 d.采用Morris水迷宫和避暗穿梭实验测定小鼠学习记忆能力,微孔板比色法测定血清和海马组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力及谷胱甘肽过氧化物酶(GSH-Px)活力.结果 Morris水迷宫实验中,各染毒组小鼠逃避潜伏期[(57.98±2.78)、(62.35±3.18)、(85.57±5.10)s]均明显高于对照组[(21.74±1.36)s],差异有统计学意义(P<0.05),并有剂量-反应关系(R=0.8629,P<0.05).避暗穿梭实验中,各染毒组小鼠主动回避潜伏期[(5.56±0.29)、(6.08±0.22)、(8.32±0.38)s]高于对照组[(3.50±0.13)s],差异有统计学意义(P<0.05),并有剂量-反应关系(R=0.9579,P<0.05).中、高剂量染毒组血清中MDA含量[(24.76±1.76)、(31.10±4.57)nmol/ml]明显高于对照组[(16.38±6.26)nmol/ml],差异有统计学意义(P<0.05);各染毒组海马组织中MDA含量[(2.26±0.18)、(2.77±0.20)、(3.37±0.39)nmol/mg Pro]明显高于对照组[(1.93±0.39)nmol/mg Pro],差异有统计学意义(P<0.05);各染毒组小鼠血清和海马组织中SOD及GSH-Px活力均低于对照组,差异有统计学意义(P<0.05).结论 百草枯可诱导的发育中小鼠海马组织氧化损伤,并导致小鼠学习记忆能力减退.

Abstract：

Objective To explore the damages of paraquat to the learning and memory ability of developing mice and explore the possible mechanism involving oxidative stress.Methods Eighty healthy Kunming mice in aged 21 days were divided into 4 groups randomly:a control group (distilled water) and three paraquat treatment groups.The doses of paraquat were 0.89,2.67 and 8mg/kg body weight,respectively.Paraquat was administered orally in doses of 0.1 ml/10 g body weight,respectively,once a day and for 28 consecutive days.The Morris water maze test and the shuttling and avoid dark box test were used to detect the learning and memory abilities of mice.The levels of MDA and the activities of SOD and GSH-PX were detected according to the commercial kits manual using a microplate reader.Results Morris water maze test showed that the escape latency of mice after paraquat treatment (57.98 ±2.78,62.35 ±3.18,85.57 ±5.10) were significantly increase compared with the control (21.74±1.36),respectively (P<0.05).There were good dose-response relationship (R=0.8629,P<0.05).The shuttling and avoid dark box test showed that initiative avoidance latency of mice after paraquat treatment (5.56 ±0.29,6.08 ±0.22,8.32 ±0.38) were significantly increase compared with the control (3.50 ±0.13),respectively (P<0.05).There were good dose-response relationship (R=0.9579,P<0.05 ).The levels of MDA in serum of mice in paraquat treatment groups (2.67 and 8mg/kg) (24.76±1.76,31.10±4.57) and in hippocampus of mice in each paraquat treatment groups were significantly increase compared with the control (serum:16.38±6.26,hippocampus:1.93±0.39) (P<0.05,respectively).The activities of SOD in serum and hippocampus of mice in each paraquat treatment groups were significantly decrease compared with the control (serum:213.25±6.78,hippocampus:197.36±6.37) (P<0.05,respectively).The activities of GSH-PX in serum and hippocampus of mice in each paraquat treatment groups were significantly decrease compared with the control (serum:583.47±11.23,hippocampus:412.38±13.16) (P<0.05,respectively).Conclusion Paraquat can induce the oxidative damage in hippocampus,and then influence the learning and memory abilities of developing mice.     

对医院护理管理队伍建设的几点思考

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医学科技成果信息资源网络开放利用的原则探析

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政府公共财政支出分配公平性研究进展

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长春新碱过量引起严重毒副反应1例的护理体会

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