利用杆状病毒早期启动子在昆虫细胞中持续表达人β—干扰素

在含苜蓿尺蠖核多角体病毒（ＡｃＮＰＶ）早期基因启动子的载体ｐＡｃＩＥ１中，插入人β－干扰素（ＨｕＩＦＮ－β）基因，构建成转移载体ｐＩＥ１－ＩＢ；ｐＩＥ１－ＩＢ与含新霉素抗性基因（ＮＥＯ＾ｒ）的质粒ｐＩＥ１－ＮＥＯ共转染秋粘虫细胞（Ｓｆ９细胞），使ＨｕＩＦＮ－β基因和ＮＥＯ＾ｒ基因整合到细胞基因组上产生转化细胞，含Ｇ４１８的培基分离新霉素抗性克隆，并增殖传代；转化细胞株传至５０代以上，不同传代水平细     

人β－干扰素在杆状病毒载体与Sf细胞体系中的表达

采用苜蓿尺蠖核多角休病毒（Ａｕｔｏｇｒａｐｈａｃａｌｉｆｏｒｎｉｃａｎｕｃｌｅａｒｐｏｌｙｈｅｄｒｏｓｉｓｖｉｒｕｓ，ＡｃＮＰＶ）ＤＮＡ中多角体蛋白基因启动子及其前后序列作为转移载体，将人β－干扰素（ＨｕＩＦＮ－β基因插入转移载体ｐＵＡｃ－５，构建成ｐＡｃ－ＩＦＮ－β载体。该质粒ＤＮＡ与野生型ＡｃＮＰＶＤＮＡ经ｌｉｐｏｆｅｃｔｉｎ共转染秋粘虫（Ｓｐｏｄｏｐｔｅｒａｆｒｕｇｉｐｅｒｄａ）传代细胞（Ｓｆ９细胞），利用重组病毒不产生多角体蛋白的特征，进行病毒空斑筛选。用核酸杂交方法鉴定出携带ＨｕＩＦＮ－β基因的主组病毒。用重组病毒感染Ｓｆ９细胞，可表达ｒＨｕＩＦＮ－β。在１．０×１０￣６细胞／ｍｌ感染上清中测定ｒＨｕＩＦＮ－β最高活性为３．０×１０￣６ＩＵ／ｍｌ，抗天然ＨｕＩＦＮ－β抗体能完全中和ｒＨｕＩＦＮ－β的抗病毒活性。     

人β—干扰素在杆状病毒载体与Sf细胞体系中的表达

采用苜蓿尺蠖核多角体病毒ＤＮＡ中多角体蛋白基因启动子及其前后序列作为转移载体，将人β－干扰素（ＨｕＩＦＮ－β）基因插入转移载体ｐＵＡｃ－５，构建成ｐＡｃ－ＩＦＮ－β载体。该质粒ＤＮＡ与野生型ＡｃＮＰＶ ＤＮＡ经ｌｉｐｏｆｅｃｔｉｎ共转染秋粘虫传代细胞（Ｓｆ９细胞），利用重组病毒不产生多角体蛋白的特征，进行病毒空斑筛选。用核酸杂交方法鉴定出携带ＨｕＩＦＮ－β基因的重组病毒。用重组病毒感染Ｓｆ９细胞，     

REGULATORY EFFECTS OF WOUND MACROPHAGE PRODUCTS ON WOUND FIBROBLASTS

ＲＥＧＵＬＡＴＯＲＹＥＦＦＥＣＴＳＯＦＷＯＵＮＤＭＡＣＲＯＰＨＡＧＥＰＲＯＤＵＣＴＳＯＮＷＯＵＮＤＦＩＢＲＯＢＬＡＳＴＳＷＡＮＧＹａｎ－ｚｈｕａｎｇ（王彦庄），ＸＵＥｒ－ｚｈｅｎ（徐尔真），ＨｅＦａｎｇ（何方），ＤＩＮＧＷｅｉ（丁薇）Ｄｅｐａｒｔｍｅ...     

肝癌干扰素-γ基因修饰瘤苗的实验研究

构建携带人干扰素－γ（ＩＦＮ－γ）ｃＤＮＡ的逆转录病毒载体ｐＺＩＰ－ＩＦＮ－γ，上清感染法导入人肝癌细胞系ＨｅｐＧ２，分泌ＩＦＮ－γ活性为每４８ｈ０￣２７３Ｕ／１０＾６。Ｓｏｕｔｈｅｒｎ Ｂｌｏｔ和Ｓｌｏｔ Ｂｌｏｔ验证目的基因的整合及表达。Ｗｅｓｔｅｒｎ Ｂｌｏｔ证明ＭＨＣⅠ类分子的表达水平增加。体外混合淋巴细胞、肿瘤细胞培养，证实该瘤苗能刺激肝癌病人外周血淋巴细胞的增殖。该瘤苗还能增强ＨＬＡＡ     

STUDY OF AN ABNORMAL PROTEIN FROM INJURED RABBITS ON IMMUNE FUNCTION

ＳＴＵＤＹＯＦＡＮＡＢＮＯＲＭＡＬＰＲＯＴＥＩＮＦＲＯＭＩＮＪＵＲＥＤＲＡＢＢＩＴＳＯＮＩＭＭＵＮＥＦＵＮＣＴＩＯＮＬＩＬｅｉ（李磊），ＷＡＮＧＺｈｅｎｇ－ｇｕｏ（王正国），ＺＨＡＮＧＹｕ－ｃｈｕｎ（张玉纯），ＨＵＣｈｅｎｇ－ｘｉａｎｇ（胡承香）Ｒｅ...     

人表皮细胞生长因子基因真核表达质粒pcDNA3—hEGF的构建

目的：构建人表皮细胞生长因子（ｈＥＧＦ）基因真核表达质粒，为深入研究ｈＥＧＦ在组织修复中的分子调节机制及临床应用提供物质基础。方法：含ｈＥＧＦ ｃＤＮＡ的ｐＵＣ１８－ｈＥＧＦ质粒于大肠杆菌ＪＭ１０９内扩增；提纯及纯化ｐＵＣ１８－ｈＥＧＦ质粒；经ＤＮＡ序列分析其所含ｈＥＧＦ ｃＤＮＡ；限制性酶切ｈＥＧＦ ｃＤＮＡ片段，连接酶连接到真核表达质粒ｐｃＤＮＡ３－ｎｅｏ内，克隆出真核表达质粒ｐｃＤＮＡ３－ｈ     

（99m）~Tc_4和（99m）~Tc－MIBI对甲状腺结节显像的比较

０４４（９９ｍ）￣Ｔｃ＿４和（９９ｍ）￣Ｔｃ－ＭＩＢＩ对甲状腺结节显像的比较［英］／ＦｏｌｅｓＩ…ＥｕｒＪＮｕｃｌＭｅｄ．－１９９３，２０．－３３０通过９９ｍＴｃＯ４和９９ｍＴｃ－ＭＩＢＩ对５８例病人行甲状腺显像，比较了ＭＩＢＩ浓集于冷热结节中与９９...     

不同时间生理刺激对躯体感觉皮层转录因子表达的影响

近年来的药理学研究表明，毒蕈碱样激动剂刺激神经原突触后膜可在神经元样的ＰＣ１２细胞中诱导即刻早期基因（ＩＥＧ，ＩｍｍｅｄｉａｔｅＥａｒｌｙＧｅｎｅ）ｃ－ｆｏｓ［１］；癫痫作为强烈的刺激亦可在活体神经元中诱导出这些ＩＥＧ［２］；近来研究着重于生理刺激是否影响ＩＥＧ在大脑皮层的表达。阻断视觉传入活动导致Ｚｉｆ２６８（ＮＧＦＩ－Ａ）在枕叶皮质的表达下降［３］；刺激胡须产生躯体感觉皮层中一些ＩＥＧ的诱导，包括ＮＧＦＩ－Ａ，ＮＧＦＩ－Ｂ，ｆｏｓ－相关抗原［４］；同样的刺激也产生了这些转录因子的靶基因，如谷…     

EFFECTS OF EMBRYONIC SPINAL CORD TRANSPLANTATION ON RECONSTRUCTION OF NERVE TRACT OF INJURED SPINAL CORD OF ADULT RATS

ＥＦＦＥＣＴＳＯＦＥＭＢＲＹＯＮＩＣＳＰＩＮＡＬＣＯＲＤＴＲＡＮＳＰＬＡＮＴＡＴＩＯＮＯＮＲＥＣＯＮＳＴＲＵＣＴＩＯＮＯＦＮＥＲＶＥＴＲＡＣＴＯＦＩＮＪＵＲＥＤＳＰＩＮＡＬＣＯＲＤＯＦＡＤＵＬＴＲＡＴＳＬＩＢｉｎｇ－ｃａｎｇ（李兵仓），ＷＡＮＧＺｈｅ...     

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version

The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

Do Balance Board Training Programs Reduce the Risk of Ankle Sprains in Athletes?

Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).     

High Intensity Interval Training:New Insights

KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief，intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.，≥90%of VO2 peak).     

Endovascular management of carotid-cavernous fistulas

Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas （CCF） and their complications such as pseudoaneurysms. Methods： Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% （4/22）. A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% （8/22）. Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents.     

Developmental validation of the PowerPlex(®) ESI 16 and PowerPlex(®) ESI 17 Systems: STR multiplexes for the new European standard

In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega^® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex^® ESI 16 (European Standard Investigator 16) and the PowerPlex^® ESI 17 Systems. The PowerPlex^® ESI 16 System combines the 11 loci compatible with the UK National DNA Database^®, contained within the AmpFlSTR^® SGM Plus^® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR^® SGM Plus^® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex^® ESI 17 System amplifies the same loci as the PowerPlex^® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR^® SGM Plus^® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex^® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors.     

Bronchial stump fistula :treatment with covered retrievable hinged metallic stents-preliminary clinical experience

Objective To evaluate the preliminaily clinical efficacy and retrievability of a retrievable hinged covered metallic stent in the treatment of the bronchial stump fistula （BSF）. Methods Between April 2003 and March 2005, 8 patients with bronchial stump fistula after pneumonectomy or lobectomy were treated with two types （A and B） of retrievable hinged covered metallic stents. Type A stent was placed in 6 patients and type B in 2 under fluoroscopic guidance. The stent was removed with a retrieval set when BSF was healed or complications occurred. Results Stent placement in the bronchial tree was technically successful in all patients, without procedure-related complications. Immediate closure of the BSF was achieved in all patients after the procedure. Stents were removed from all patients but one. Removal of the stents was difficult in two patients due to tissue hyperplasia. Patients were followed up for 6 - 21 months. Placement of the stents remained stable in all patients except one due to severe cough. Permanent closure of BSF was achieved in 7 （87.5%） of 8 patients. Conclusion Use of a retrievable hinged covered expandable metallic stent is a simple, safe, and effective procedure for closure of the BSF. Retrieval of the stent seems to be feasible. （J Intervent Radiol, 2007, 16： 253-257）     

Prevalence and severity of hip and groin pain in sub‐elite male football: a cross‐sectional cohort study of 695 players

The purpose of this study was twofold: (a) to investigate the prevalence of hip and groin pain in sub‐elite male adult football in Denmark and (b) to explore the association between prevalence and duration of hip and groin pain in the previous season with the Copenhagen Hip and Groin Outcome Score (HAGOS) in the beginning of the new season. In total 695 respondents from 40 teams (Division 1–4) were included. Players completed in the beginning of the new season (July–Sept 2011) a self‐reported paper questionnaire on hip and/or groin pain during the previous season and HAGOS. In total 49% (95% CI: 45–52%) reported hip and/or groin pain during the previous season. Of these, 31% (95% CI: 26–36%) reported pain for >6 weeks. Players with the longest duration of pain during the previous season had the lowest HAGOS scores, when assessed at the beginning of the new season, P < 0.001. This study documents that half of sub‐elite male adult football players report pain in the hip and/or groin during a football season. The football players with the longest duration of pain in previous season displayed the lowest HAGOS scores in the beginning of the new season.