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1.
冬虫夏草多糖治疗慢性丙型肝炎患者的临床研究 总被引:14,自引:2,他引:14
目的研究冬虫夏草多糖(CP)治疗慢性丙型肝炎的疗效.方法慢性丙型肝炎患者21例,口服CP15mL,3次/d,连服3mo,治疗前后检测肝功能、血清肝纤维化标志物外周血T细胞亚群及NK活性的变化.结果慢性丙型肝炎患者经CP治疗后,血清ALT(U/L,61±35vs35±15)及rGT(U/L,169±85vs118±52)较治疗前显著降低(P<005).血清HA(μg/L,293±109vs214±96)、PⅢP(μg/L,143±48vs114±42)及CⅣ(μg/L,245±98vs188±87)均较治疗前显著下降(P<001,<005及<005);CD4(364%±66%vs410%±56%)、CD4/CD8(114±040vs143±022)、NK(167%±46%vs197%±42%)均较治疗前显著增加(P<001),而CD8(326%±47%vs289%±37%)则明显降低(P<005);血清胆红素略减、清蛋白略增但差异均无显著性.结论冬虫夏草多糖可以增强慢性丙型肝炎患者细胞免疫功能,改善肝功能,并具有一定的抗纤维化作用 相似文献
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门脉高压大鼠门静脉及周围血NO水平观察 总被引:2,自引:0,他引:2
目的了解门脉高压鼠血清一氧化氮(NO)含量变化及意义.方法以部分门静脉结扎大鼠为模型(n=12),在部分门静脉结扎及假手术组大鼠(n=8)术后2周取门静脉血和周围静脉血,采用荧光分析法测量NO-2含量反应NO水平.结果门脉高压组门静脉血NO-2为0766μmol/L±0097μmol/L,周围静脉血为0687μmol/L±0092μmol/L,两者比较相差显著(P<001);对照组门脉血NO-2为0613μmol/L±0084μmol/L,周围血为0591μmol/L±0045μmol/L,二者无明显区别(P>005).门脉高压组与对照组比较,门脉血和外周血中NO-2含量均显著高于对照组(P<001).结论门脉高压大鼠血清NO-2浓度升高,尤以门静脉血含量升高显著,表明门脉高压大鼠血中NO生成增多,可能在门脉高压症发病中具有一定作用. 相似文献
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目的探讨导致糖尿病酮症(DK)及酮症酸中毒(DKA)患者肝损害的相关因素.方法DK或DKA患者99例,其中ALT及AST均异常升高11例(A组),单项ALT异常升高13例(B组),肝功能正常75例(C组),对以上各组患者的血二氧化碳结合力(CO2CP)、尿素氮(BUN)、血糖(BG)和血浆渗透压(OSM)进行了统计分析.结果A,B两组患者的CO2CP明显低于C组(P<001,t=633和t=643),而BUN则明显升高(P<001,t=361,AvsC;P<001,t=435,BvsC),A组的BG(P<005,t=284)和血浆OSM(P<005,t=310)水平也显著高于C组,而B组患者的BG及血浆OSM与C组比较无差异;与B组相比,A组患者的CO2CP明显降低(P<002,t=271),BG(P<005,t=289)和血浆OSM(P<005,t=236)明显升高.此外,Ⅰ型糖尿病患者血清转氨酶异常升高的发生率明显高于Ⅱ型糖尿病患者(P<005,χ2=438).结论酸中毒和脱水是导致糖尿病酮症及酮症酸中毒患者肝损害的重要因素,酸中毒及脱水程度与肝损害程度相关. 相似文献
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亚硒酸钠对胃粘膜细胞非程序DNA合成、LPO和ras P21表达的影响 总被引:1,自引:1,他引:1
目的研究亚硒酸钠对甲基硝基亚硝基胍(MNNG)所致胃粘膜细胞损伤的防护作用.方法观察了亚硒酸钠对MNNG所致胃粘膜细胞非程序DNA合成(UDS)、脂质过氧化物(LPO)和rasP21表达的影响.结果胃粘膜细胞先用10μmol/L或1μmol/L亚硒酸钠预处理4h,再给MNNG组细胞的非程序DNA合成水平(cpm/×10-6min-1,1166±156或1566±187vs1838±205,P<001~005),脂质过氧化物(20d,μmol/L,45±06或47±06vs74±07,P<001)和rasP21蛋白含量(20d,A,068±008或086±007vs108±011,P<001~005)均显著低于MNNG组.结论一定剂量亚硒酸钠对MNNG诱导的胃粘膜细胞损伤有防护作用. 相似文献
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老年胃酸分泌与生长抑素的研究 总被引:2,自引:2,他引:2
目的探讨老年胃泌酸功能与生长抑素的调节机制.方法我室应用RIA法对20例老年人和15例青年人胃液及9只Wistar老年鼠,20只青年鼠胃粘膜组织、血浆生长抑素含量进行测定,以及对老年人和青年人进行胃酸分泌试验.结果老年人组比青年组胃液生长抑素呈高水平分泌,空腹胃液SS为(1365±443)ng/Lvs(859±345)ng/L,P<001;刺激后SS为(1967±664)ng/Lvs(1141±1073)ng/L,P<001.老年人比青年人胃酸分泌量明显减少,BAO为(35±21)mmol/hvs(69±44)mmol/h,P<001;PAO为(136±64)mmol/hvs(208±112)mmol/h,P<005.不同年龄Wistar大鼠胃粘膜组织生长抑素具有不同的生理浓度,在老龄鼠比青年鼠SS也呈高水平分布,为(4525±1842)ng/g蛋白vs(1509±938)ng/g蛋白,P<001.结论老年胃泌酸功能改变与生长抑素调节作用存在重要关系. 相似文献
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缺血预处理对大鼠肝缺血再灌注损伤的保护 总被引:1,自引:3,他引:1
目的反复短时间缺血可保护组织免受随之而来的长时间缺血性损伤,这种现象称之为缺血预处理(IPC).现探讨IPC在肝脏缺血再灌注(I/R)损伤中的作用及机制.方法采用大鼠肝脏原位I/R模型,术前腹腔注射L精氨酸(LArg)250mg·kg-1或L硝基精氨酸(LNNA)15mg·kg-1,分别观察血一氧化氮(NO),ALT,AST;肝组织NO,脂质过氧化物(LPO)、超氧化物歧化酶(SOD)及肝组织病理损伤.结果肝组织LPO含量(多少vs多少)、血清ALT(多少vs多少)、AST(多少vs多少)活性明显低于I/R组,IPC显著改善了由I/R引起的肝脏损伤.这种保护作用完全被NO供体(LArg)或NO合酶抑制剂(LNNA)消除.同时,各组之间血、肝NO含量无显著差异.结论IPC对肝脏I/R损伤呈现出明显的保护作用,其机理是灭活了氧自由基.NO在肝脏IPC中似乎无明显作用. 相似文献
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电针足三里穴对应激性胃溃疡大鼠一氧化氮和儿茶酚胺的影响 总被引:1,自引:0,他引:1
目的研究电针足三里穴对束缚———冷冻应激性胃溃疡大鼠一氧化氮、多巴胺和去甲肾上腺素的影响.方法应用生物化学方法分析胃窦、胃体粘膜和血液中NO、DA和NE含量在电针前后的变化,随机分为四组:应激性胃溃疡组、应激后电针组、先电针后应激组和对照组进行观察.结果①应激性胃溃疡大鼠血清NO含量(x±s,578±149μmol/L)比对照组(1330±275)非常显著下降,P<001;胃窦粘膜DA含量显著下降(331±067vs678±465),P<005,胃体粘膜DA呈增高趋势.②电针足三里穴引起应激性胃溃疡大鼠NO水平回升(791±111),与应激组相比,P<001.电针引起胃窦和胃体粘膜DA及NE含量改变,有双向调节作用,即原降低者上升、原升高者下降,分别与应激组相比,均为P<001.结论电针对胃粘膜具有保护作用,它是通过针刺对DA和NE的双向调节,发挥了DA的调控作用,影响NE的水平,通过NO参与的舒血管作用,调节血流量、增强粘膜防御能力而实现的. 相似文献
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内皮素与一氧化氮在肝硬变血流动力学紊乱中的作用 总被引:4,自引:9,他引:4
目的研究内皮素、一氧化氮在肝硬变血流动力学紊乱中的作用及关系.方法应用放免法和高效液相色谱法分别检测肝硬变患者44例,(男32例,女12例;年龄504岁±110岁),其中腹水患者27例及健康对照25例(男18例,女7例;年龄468岁±124岁)血浆内皮素(ET)、一氧化氮(NO)及部分血管活性物质水平.结果肝硬变组血浆ET及NO水平(570ng/L±254ng/L,3872μg/L±1064μg/L)明显高于对照组(330ng/L±109ng/L,2923μg/L±545μg/L,P<001).腹水患者血浆ET及NO水平(675ng/L±247ng/L,4147μg/L±1071μg/L),显著高于无腹水患者(459ng/L±183ng/L,3272μg/L±702μg/L,P<001).NO与ET呈直线正相关(r=0772,P<001).结论肝硬变患者ET与NO水平升高,且腹水患者较无腹水者更升高;两者呈直线正相关 相似文献
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大肠癌患者促胃液素检测的临床意义 总被引:1,自引:1,他引:1
目的研究大肠癌患者血清及癌细胞内促胃液素(Gas)水平及临床意义.方法采用RIA法检测35例大肠癌患者血清和癌细胞及癌旁粘膜细胞内Gas水平.结果大肠癌患者术前、术后血清Gas水平与对照组无显著差异(P>005),根治术后明显低于术前(29ng/L±5ng/Lvs35ng/L±12ng/L,t=2772,P<001),在高分化(36ng/L±16ng/Lvs28ng/L±5ng/L)和中分化腺癌组中(38ng/L±7ng/Lvs27ng/L±3ng/L)差异显著(t=2152和2356,P<005).大肠癌细胞内Gas水平明显高于癌旁3cm和6cm粘膜(213ag/细胞±72ag/细胞vs147ag/细胞±36ag/细胞,139ag/细胞±32ag/细胞;t=4891和5613,P<001)和正常粘膜(136ag/细胞±46ag/细胞;t=2534,P<005),高分化腺癌明显高于低分化和粘液腺癌(241ag/细胞±78ag/细胞vs161ag/细胞±46ag/细胞,t=2505,P<005).结论大肠癌细胞可通过自分泌方式分泌Gas,Gas升高是大肠癌分化良好的标志 相似文献
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雷公藤多甙对大鼠急性肝衰竭的保护作用 总被引:1,自引:1,他引:1
目的探讨雷公藤多甙(GTW)对实验性肝衰竭的保护作用.方法雄性Wistar大鼠24只,随机分为对照组(n=8),急性肝衰竭(AHF)组(n=8)和GTW保护组(n=8).GTW组在实验前5dGTW25mg/(kg·d)经胃管灌胃,其余两组均以等量生理盐水溶液灌胃.从第6天开始,AHF组和GTW组均在空腹12h后ipDGalN16g/kg,注射后40h,以30g/L戊巴比妥钠(40mg/kg)ip麻醉,心脏抽血测定血清ALT,TB和T淋巴细胞亚群.同时,在光镜和电镜下观察各组肝组织病理变化.结果AHF组ALT(IU/L)和TB(μmol/L)分别为7828±5762和125627±67027;OX8水平为140%±3%.GTW组ALT和TB分别为359±54和47±35;OX8为43%±4%.两组比较有显著统计学差异(P<005或001).同时,前者电镜下线粒体和内质网肿胀、破损,核内染色质凝聚,后者细胞器受损明显减轻.结论雷公藤多甙对实验性急性肝衰竭具有保护作用. 相似文献
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AIM: To study the immunoreactivity of the Chinese medicine Shenrouyangzhentang to vasoactive intestinal polypeptide (VIP) and its therapeutic mechanism.METHODS: The immunoreactivity of the Chinese medicine Shenrouyangzhentang to VIP was detected in the plasma of 20 normal people and 20 patients with Piyinxu (Spleen Yin deficiency) using the radioimmunoassay (RIA) method.RESULTS: The maximum binding rate B0/T was 53.29%, the non-specific binding rate N0/T was 1.170%, and the VIP standard curve was Y = 0.81983 + 0.44319X - 0.28927X2, R2 = 0.990. The VIP content in Shenrouyangzhentang was 106.6 ng/L ± 20 ng/L), while it was 90.16 ng/L ± 15 ng/L in normal human plasma and 63.25 ng/L ± 11 ng/L in the plasma of Pixinxu patients. The difference between normal plasma and Pixinxu patient plasma was statistically significant (P < 0.05).CONCLUSION: The Chinese medicine Shenrouyangzhentang demonstrated VIP immunoreactivity similar to that of normal plasma. The (vasoactive intestinal polypeptide) VIP content in Pixinxu patient plasma was lower than that in healthy subjects (P < 0.05). 相似文献
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急性心肌梗死生化指标的研究现状 总被引:4,自引:0,他引:4
近年,急性心肌梗死(AMI)生化指标检测出现了许多新的变化,主要体现三个方面:(1)测定心肌酶谱转向特异蛋白测定;(2)单一诊断转向尽早诊断与预后判断;(3)实验室分析转向实时测定。 生化指标检测对急性心肌梗死的诊断1.心肌酶学:肌酸激酶(CK)、天门冬氨酸转氨酶、乳酸脱氢酶及其同工酶等指标由于对心肌损伤的特异性差,组织分布广,被具有高度特异性的CK同工酶(CK-MB)所取代。于90年代初,CKMB成为诊断AMI的金标准。偶有保留CK,不过仅偶有保留作观察对照,且取高临界值(≥2倍)。实际上,该取值不完全适合AMI的个体诊断[1]… 相似文献
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中药复方 慎柔养真汤 中血管活性肠肽的探讨 总被引:3,自引:1,他引:3
目的中药复方慎柔养真汤中是否含有血管活性肠肽(VIP),以探索该药治疗消化系统疾病的作用机理.方法用放射免疫分析药盒,对中药复方慎柔养真汤煎剂,20例正常人和20例脾阴虚证患者血浆中VIP的含量进行检测.结果最大结合率B0/T=53290%;非特异性结合率N/T=1170%;拟合曲线方程Y=081983+044319X028927X2,拟合度R2=0990;中药中VIP=1066ng/L±20ng/L,与正常人相比较无显著性差异,但有升高趋势;正常人血浆中VIP=9016ng/L±15ng/L;脾阴虚患者血浆中VIP=6325ng/L±11ng/L,与正常人相比较有显著性差异(P<005).结论中药复方慎柔养真汤煎剂中有VIP或VIP前体复合物,其VIP水平略高于正常人血浆,而脾阴虚患者血浆中VIP水平低于正常人. 相似文献
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Dysfunction of peripheral blood dendritic cells from patients with chronic hepatitis B virus infection 总被引:63,自引:0,他引:63
Wang FS Xing LH Liu MX Zhu CL Liu HG Wang HF Lei ZY 《World journal of gastroenterology : WJG》2001,7(4):537-541
AIM: To identify the property of dendritic cells (DCs) of peripheral blood monocytes (PBMC) in patients with chronic HBV infection. METHODS: Twenty patients with persistent HBV infection were included in this study, 10 healthy subjects being used as a control group. The peripheral blood mononuclear cells (PBMC) of T cell-depleted populations were incubated and induced into mature dendritic cells in the RPMI-1640 medium in the presence of cytokines GM-CSF, IL-4, FLt-3,TNF-alpha and 100mL.L(-1 )of fetal calf serum for a total of 10-12 days. The expressions of surface markers on DCs were evaluated using flow cytometric analysis. ELISA method was used to determine the cytokine levels of interleukin-12 (IL-12) and IL-10 in the supernatant produced by DCs. For detection of the stimulatory capacity of DCs to T cell proliferation, mytomycin C-treated DC were incubated with allogenic T cells. RESULTS: A typical morphology of mature DCs from healthy subjects and HBV-infected patients was induced in in vitro incubation, but the proliferation ability and cellular number of DCs from HBV-infected patients significantly decreased compared with healthy individuals. In particular, the expression levels of HLA-DR, CD80 (B7-1) and CD86 (B7-2) on DC surface from patients were also lower than that from healthy individuals (0.46 vs 0.92 for HLA-DR, 0.44 vs 0.88 for CD80 and 0.44 vs 0.84 for CD86,P<0.05). The stimulatory capacity and production of IL-12 of DCs from patients in allogenic mixed lymphocyte reaction (AMLR) significantly decreased, but the production level of nitric oxide (NO) by DCs simultaneously increased compared with healthy subjects (86 +/- 15 vs 170 +/- 22 micromol.L(-1), P <0.05). CONCLUSION: The patients with chronic HBV infection have the defective function and immature phenotype of dendritic cells, which may be associated with the inability of efficient presentation of HBV antigens to host immune system for the clearance of HBV. 相似文献
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Cai Yah Zhao Yue Lin Li Su Xia Liu Zhong Jun Feng 《World journal of gastroenterology : WJG》2000,6(3)
AIM To study the changes of IL-6,IL-2, sIL-2R and TNF ir patients with hepatocellular carcinoma(HCC)and their clinical significance.METHODS IL-6, IL-2, sIL-2R and TNF were detected by avidin-biotin-system ELISA, double-sandwichELISA respectively in 60 patients with HCC and 36 patients with liver cirrhosis (LC) and 66 healthy persons.RESULTS The levels of IL-6, sIL-2R and TNF increased, but IL-2 level was lower in patients with HCCthan that in normal controls (NC) (t test, t=8.21, 4.71, 3.87, 2.13, P<0.01 or 0.05). IL-6 level in HCCwas 10 fold higher than NC, and also much higher than LC. IL-6 level was higher in later stage than that inearlier stage. There was a positive correlation between IL-6 and sIL-2R, TNF, while no positive correlationwas found between IL-2 and IL-6, sIL-2R in HCC.CONCLUSION The remarkably higher level of IL-6 is helpful for the early diagnosis of HCC. 相似文献
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探讨联合测定血清GLS和AFP对肝癌诊断的临床意义。测定肝癌、非癌肝病患者血清神经节苷脂(GLS)和AFP含量,然后分组统计、分析结果,肝癌组GLS(799±226)mg/L显著高于非癌患者(481±104),后者与正常人(466±65)无明显差异。测定血清GLS对肝癌诊断敏感度、特异度、准确度、诊断效率分别为82.4%、94.7%、89.7%和78.0%,具有显著临床价值。肝癌组GLS和/或AFP升高为阳性者达97.3%,显著高于良性肝病组,可用于初筛肝癌;GLS与AFP都升高为阳性者达到52.7%,而非癌肝病和正常人组都为0,可用于确诊肝癌。结果显示肝癌患者血清GLS显著增多;测定血清GLS对肝癌诊断、监测及预后分析具有显著临床价值。血清GLS与AFP联合测定对肝癌诊断具有更显著的临床意义。 相似文献
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血清雌激素与肝细胞癌转移的关系 总被引:2,自引:2,他引:2
目的了解血清雌激素升高与原发性肝细胞癌(HCC)转移的关系.方法应用放射免疫分析法(RIA)对HCC患者血清雌二醇(E2)含量进行了检测,并应用原位杂交研究外源性E2对肝癌细胞系CBRH7919血管内皮生长因子(VEGF)和层粘蛋白受体(LNR)表达的影响.结果HCC患者血清E2水平较正常者显著升高,且HCC有转移者血清E2水平较无转移者亦显著升高;原位杂交结果显示,外源性E2能够增加CBRH7919细胞表达VEGF和LNRmRNA.结论血清E2水平增高与HCC转移密切相关,它有可能通过促进VEGF和LNR的合成而影响肿瘤的转移过程. 相似文献
20.
Xian Yong Bai Xiu Hong Jia Ling Zhong Cheng Yun Di Gu 《World journal of gastroenterology : WJG》2001,7(3)
!NTRODUCTIONKuPffer eells are residential macroPhages in the1 iver,which Play a eritieal role in the maintenaneeof normal liver function and in immunal surveilaneeof hePatoeellular earcinoma(HCC)and othereaneers〔l].The bio一ogieal immune modulants havebeen used for treating Patients with HCC and othereaneers[2〕.In our previous studies,the eombineduse of biological immune modulants showed bettereffeets.The normal rats and hePatoma rats indueedby DEN(Diethylnitrosamine)were treated … 相似文献