长期力竭运动动情周期抑制模型大鼠卵巢组织形态和超微结构的变化

背景：研究运动性月经失调女性性腺和卵巢的功能,以及形态学的改变对于探讨运动性月经失调的发生机制具有重要意义。目的：通过建立长期力竭运动致动情周期抑制大鼠模型,观察卵巢的组织形态和细胞超微结构的变化。方法：正常3月龄SD雌性大鼠随机分成对照组和模型组,其中模型组大鼠每日进行一次力竭游泳运动直至其动情周期紊乱,取卵巢组织,苏木精-伊红染色,光镜下观察卵巢组织形态变化,电镜下观察卵巢细胞超微结构的变化。结果与结论：与对照组相比,模型组大鼠卵巢典型生长卵泡和新鲜黄体明显减少,原始卵泡、闭锁卵泡则相对增加,其颗粒细胞、黄体细胞内细胞器明显减少而含有大量的脂滴。提示长期力竭运动可抑制大鼠卵巢卵泡的发育、成熟、排卵与黄体形成,并使卵巢细胞的超微结构发生抑制性改变。     

雌性大鼠运动性动情周期抑制与卵巢、子宫显微结构及相关激素水平的变化

目的:观察长时间大强度耐力运动对青春期雌性大鼠的动情周期、卵巢和子宫的显微结构及其相关激素水平表达的影响。方法:实验于2004-03/10在山东体育学院实验中心完成。选择有正常动情周期的健康2月龄雌性SD大鼠40只,数字表法随机分为对照组、大强度运动组(n=20)。大强度运动组采用递增负荷强度的跑台运动,在中国杭州段氏PT200跑台上进行,运动1h/d,每周运动6d,持续训练10周;对照组不施加干预,饲养笼内自由活动。训练过程中每天观察两组大鼠动情周期的变化,持续10周后,当大强度运动组大鼠的动情周期长时间停留在动情间期时,即发生动情周期抑制时,和对照组一起处死,采血并分离卵巢、子宫组织,光镜观察卵巢和子宫的显微结构变化,采用放免法测定血清雌激素、孕激素、胰岛素样生长因子1水平。结果:35只大鼠进入结果分析。①大强度运动组大鼠的动情周期均长时间停留在动情间期,表现为动情周期抑制现象。②与对照组相比,运动性动情周期抑制雌性大鼠的卵泡变小且数量变少、子宫腺数量变少且腺腔小。③运动性动情周期抑制雌性大鼠的血清雌激素、胰岛素样生长因子1和孕激素水平显著低于对照组[(17.54±9.72),(24.32±10.38)ng/L,P<0.05;(1016.08±269.38),(1285.65±297.77)μg/L,P<0.05;(26.84±14.09),(49.64±23.88)nmol/L,P<0.01]。结论:①长时间大强度运动引起雌性大鼠发生了运动性动情周期抑制现象,导致卵巢、子宫结构的改变。②血清雌激素、孕激素分泌的降低是运动性动情周期抑制的重要原因,胰岛素样生长因子1水平与运动性动情周期抑制的发生密切相关。     

艾灸肾俞穴对肾虚大鼠海马DG区组织形态及细胞超微结构的影响

目的：探讨艾灸肾俞穴对肾虚大鼠海马齿状回区组织形态及细胞超微结构的影响。方法将16只月龄10个月、连续配种6个月、经鉴定生殖功能下降的远交群大鼠随机分为模型组和研究组,8只月龄2个月的大鼠作为对照组。研究组给予艾灸肾俞穴治疗,模型组和对照组不予治疗。30 d后处死取材,光镜下观察海马齿状回区组织形态变化,透射电镜下观察大鼠海马齿状回区细胞超微结构变化。结果对照组大鼠海马齿状回区组织形态及细胞超微结构基本正常,模型组大鼠海马齿状回区存在组织形态及细胞超微结构病变,研究组大鼠海马齿状回区组织形态及细胞超微结构病变明显减轻。结论长期房室不节可引起大鼠海马齿状回区组织形态及细胞超微结构病变,艾灸肾俞穴有改善肾虚大鼠海马齿状回区组织形态及细胞超微结构病理改变的作用。     

雌性大鼠运动性动情周期抑制与卵巢、子宫显微结构及相关激素水平的变化

目的：观察长时间大强度耐力运动对青春期雌性大鼠的动情周期、卵巢和子宫的显微结构及其相关激素水平表达的影响。 方法：实验于2004—03／10在山东体育学院实验中心完成。选择有正常动情周期的健康2月龄雌性SD大鼠40只，数字表法随机分为对照组、大强度运动组（n=20）。大强度运动组采用递增负荷强度的跑台运动，在中国杭州段氏PT200跑台上进行，运动1h／d，每周运动6d，持续训练10周；对照组不施加干预，饲养笼内自由活动。训练过程中每天观察两组大鼠动情周期的变化，持续10周后，当大强度运动组大鼠的动情周期长时间停留在动情间期时，即发生动情周期抑制时，和对照组一起处死，采血并分离卵巢、子宫组织，光镜观察卵巢和子宫的显微结构变化，采用放免法测定血清雌激素、孕激素、胰岛素样生长因子1水平。 结果：35只大鼠进入结果分析。①大强度运动组大鼠的动情周期均长时间停留在动情间期，表现为动情周期抑制现象。②与对照组相比，运动性动情周期抑制雌性大鼠的卵泡变小且数量变少、子宫腺数量变少且腺腔小。③运动性动情周期抑制雌性大鼠的血清雌激素、胰岛素样生长因子1和孕激素水平显著低于对照组[（17．54&;#177;9．72），（24．32&;#177;10．38）ng／L，P〈0．05；（1016．08&;#177;269．38），（1285．65&;#177;297．77）μg／L，P〈0．05；（26．84&;#177;14．09），（49．64&;#177;23．88）nmoL／L,P〈0．01]。 结论：①长时间大强度运动引起雌性大鼠发生了运动性动情周期抑制现象，导致卵巢、子宫结构的改变。②血清雌激素、孕激素分泌的降低是运动性动情周期抑制的重要原因，胰岛素样生长因子1水平与运动性动情周期抑制的发生密切相关。     

力竭性运动对大鼠血浆心钠素、内皮素及心肌血供的影响

目的：研究不同训练程度大鼠力竭性运动后血浆心钠素（ANP）、内皮素（ET）含量的变化，观察运动后其心肌组织的缺血状况。方法：雄性SD大鼠随机分3组：对照组、无训练游泳力竭组、长期耐力训练游泳力竭组。安静对照组笼内生活，自由饮食；无训练游泳力竭组适应性喂养1周后进行一定的适应性游泳训练．之后与安静对照组饲养方式相同；长期耐力训练游泳力竭组训练8周，每周6次。8周后进行力竭性运动，运动后取大鼠血浆，测定ANP、ET含量，取心肌组织，做光镜切片，观察缺血情况。结果：两力竭组大鼠血浆ANP、ET含量与对照组相比发生显著性改变，其心肌组织皆有缺血现象发生，且无训练组较训练组严重。结论：长期耐力游泳训练可使大鼠心脏结构及功能产生适应性改变，力竭性运动会造成大鼠心血管内分泌活性物质ANP、ET拮抗功能的失调，并造成心肌组织缺血。     

游泳运动对大鼠心房肌细胞超微结构的影响

目的:探讨不同运动量游泳运动对大鼠心房肌细胞超微结构的影响,以期为运动对心肌的生理和病理变化研究提供参考资料.方法:SD大鼠分为不同运动量运动组和力竭运动组,前者进行12周的低、中和高运动量游泳运动,后者进行1次力竭游泳运动.取右心房肌组织进行常规透射电镜样品制备,透射电镜观察心房肌细胞超微结构的变化.结果:低和中运动量运动可使肌原纤维排列整齐,Z线变粗,Ⅰ带增宽;线粒体数量增加,嵴密集排列;内质网和高尔基体丰富,合成大量心钠肽,并快速地分泌到细胞外;细胞连接增强.高运动量运动,导致肌原纤维部分Z线紊乱,核膜内陷,线粒体嵴疏松,细胞连接减弱.力竭运动对肌原纤维损伤较重,细胞核染色质固缩凝集,线粒体形态改变,嵴消失;但肌原纤维和线粒体的形态结构在力竭后24h得以部分恢复.结论:高运动量和力竭运动可以导致心房肌超微结构破坏.     

力竭运动后24 h模型大鼠肾脏结构和炎症因子对黑加仑提取物干预的反应

背景：急性运动后肾脏组织易出现运动性肾缺血再灌注现象,自由基生成增加,发生炎症反应,导致肾功能异常。目的：观察黑加仑提取物对力竭运动后24 h大鼠肾脏结构、肿瘤坏死因子α和核因子κB表达的影响。方法：30只雄性Wistar健康大鼠随机分为3组,每组10只,黑加仑提取物组给予0.44 g/kg黑加仑提取物灌胃,安静对照组、力竭运动后24 h组灌服同等体积的蒸馏水,连续灌胃6周。力竭运动后24 h组和黑加仑提取物组于末次灌胃后进行无负重游泳运动直至力竭疲劳,于24 h后取材,电镜和光镜观察肾脏组织形态和超微结构变化,免疫组化法检测肿瘤坏死因子α蛋白和核因子κB蛋白表达,RT-PCR检测肿瘤坏死因子α mRNA和核因子κB mRNA的表达。结果与结论：与安静对照组比较,力竭运动后24 h组中肾脏核因子κB蛋白和肿瘤坏死因子α蛋白高表达,核因子κB mRNA和肿瘤坏死因子α mRNA表达水平极显著增加（P 〈0.01）;与力竭运动后24 h组比较,黑加仑提取物组中肾脏核因子κB蛋白和肿瘤坏死因子α蛋白低表达,核因子κB mRNA表达水平显著性降低（P 〈0.05）;肿瘤坏死因子α mRNA表达水平极显著性下降（P〈0.01）。力竭运动后24 h组大鼠肾脏发生明显的形态变化和超微结构损伤,黑加仑提取物组大鼠肾脏结构趋于正常。结果表明黑加仑提取物可修复力竭运动后24 h肾脏组织结构,减少炎症因子的表达,防止肾脏发生炎症性损伤。     

槲皮素对大鼠卵巢发育和卵巢寿命的影响

背景:研究发现一些植物雌激素可改变生殖系统发育,并可能最终影响生殖衰老的年龄.槲皮素是一种黄酮类物质,具有微弱的雌激素效应,但对卵巢卵泡发育和卵巢衰老进程究竟有无影响还不清楚.目的:观察槲皮素对大鼠卵巢卵泡发育和卵巢寿命的影响.方法:健康成年SD大鼠30只雌雄按2:1合笼所生的新生雌鼠,分为3组:母鼠灌胃组,对孕10.5 d母鼠槲皮素灌胃,1次/d,直至分娩,取1,2,4 d新生雌鼠;腹腔注射组,对正常出生后12 h内新生雌鼠槲皮素腹腔注射,1次/d,取2,4 d新生雌鼠:空白对照组:不进行任何干预的1,2,4 d新生雌鼠;观察新生鼠卵巢发育情况.另取12月龄雌性大鼠22只,以数字表法随机分为实验组和对照组:实验组用槲皮素灌胃4个月/1次/d.对照组灌胃给予生理盐水.对灌胃前和灌胃期间12月龄雌性大鼠行阴道涂片,观察大鼠动情周期模式:灌胃结束后,计算卵巢和子宫系数,苏木精一伊红染色观察各期卵泡发育情况和卵泡总擞变化.结果与结论:用槲皮索干预1,2 d龄新生大鼠未装配的卵泡均减少,而发育更晚期阶段的卵泡即原始卵泡或发育卵泡有所增加.灌胃前大部分大鼠动情周期已经不规则,用药后,随着大鼠年龄的增长,实验组与对照组显示了相似的动情周期变化:规则的动情逐渐消失,周期变得延长或不规则,灌胃结束时均以不规则动情为主.灌胃4个月后,实验组大鼠卵巢大部分由闭锁卵泡组成,另外可见少许原始卵泡、发育卵泡以及窦状卵泡和黄体,实验组窦状卵泡较对照组增加(P<0.05),两组卵巢和子宫系数及体质量增加无差异.提示槲皮素可能具有一定促进卵巢卵泡发育和成熟的作用,但不影响卵巢寿命.     

大鼠胚胎卵巢冷冻保存和异体无血管皮下移植的生长

背景:胚胎卵巢组织免疫原性低,不易引起宿主的免疫排斥反应,是卵巢异体移植的理想供体.但由于获取胎儿卵巢的不随意性和来源限制,标本的冷冻显得至关重要,成功的冷冻保存和复苏技术是建立胎儿卵巢库的关键.目的:通过对大鼠新鲜或冻融胚胎卵巢组织异体无血管移植后的组织形态和功能的观察,探讨胚胎卵巢组织异体移植和超速冷冻保存的可行性.设计、时间及地点:随机对照动物实验.于2006-03/05在云南省第一人民医院生殖遗传科进行课题设计,于2006-05/2007-02在云南大学单克隆抗体研究中心完成实验.材料:卵巢来源于孕龄为18～20 d SD大鼠胚胎.取阴道脱落细胞涂片证实有正常动情周期未交配过的SD成年雌性大鼠作为移植对象,分4组:正常对照组(n=10)、去势对照组(n=10)、新鲜胚胎卵巢移植组(n=25)、冷冻胚胎卵巢移植组(n=25).方法:正常对照组仅行皮肤筋膜肌肉切开缝合术,去势对照组切除双侧卵巢组织.将孕龄为18～20 d的新鲜或经超速冷冻法冷冻复苏的大鼠胚胎卵巢植入去势后4周的大鼠颈部皮下作为实验组,分别为新鲜移植组和冷冻移植组.主要观察指标:通过阴道脱落细胞的观察和血清雌二醇、孕酮的测定了解大鼠内分泌恢复情况,并对移植卵巢进行组织学观察.结果:新鲜移植组和冷冻移植组分别有52.17%和38.01%大鼠恢复了动情周期,两组血清雌二醇和孕酮在分别在35和49 d恢复到正常,且动情周期天数与正常对照组比较,差异无显著性意义.冷冻移植组大鼠恢复动情周期虽稍短于新鲜移植组,但差异并无统计学意义.63 d后取出移植物观察,见移植物色泽红润,表面有丰富的血管形成,镜下见卵巢内有大量的小动脉和小静脉;移植物组织学检查发现卵泡的发育,光镜下可见不同发育阶段的卵泡,有成熟卵泡和黄体形成,形态与正常卵巢无明显差别.结论:大鼠胚胎卵巢异体异位无血管皮下移植后能存活、生长发育并具有与正常卵巢相似的内分泌功能;超速冷冻法能较好的保存胚胎卵巢的活性,复苏后与新鲜胚胎卵巢组织具有相同的生理活性.     

大鼠卵巢组织的冷冻移植

背景:玻璃化冷冻是应用高浓度的冷冻保护液结合极快的制冷速度,使细胞快速冷冻,避免细胞内外冰晶形成对细胞的破坏作用,是一种比较新的冷冻方法,具有操作简单、冷冻效率高等特点.目的:采用玻璃化冷冻技术冷冻大鼠卵巢组织,比较4种冷冻保护剂对大鼠卵巢组织学和功能的影响.方法:将大鼠随机数字表法分为6组,每组6只:二甲基亚砜+乙二醇组、二甲基亚砜+乙二醇+蔗糖组、二甲基亚砜+乙二醇+蔗糖+乙酰胺组、乙二醇+蔗糖+乙酰胺组、去势组、正常对照组.4冷冻组摘除卵巢后应用相应的冷冻保护液冻存,去势组仅摘除卵巢不冻存、不移植,正常对照组不作处理.冻存2周后解冻复苏行同体异位移植,将卵巢组织植入大鼠后肢大腿内侧.移植后30 d,观察阴道上皮类型及动情周期;移植后3个月,经腹主动脉采血检测血清雌二醇水平;回收卵巢组织做组织学检查.结果与结论:4冷冻组均可见卵巢组织结构受损表现,原始卵泡的形态正常率分别为67.9%,71.6%,80.5%,59.4%,初级卵泡形态正常率分别是41.6%,52.3%,55.9%,36.7%.二甲基亚砜+乙二醇+蔗糖+乙酰胺组卵泡正常率较二甲基亚砜+乙二醇组、乙二醇+蔗糖+乙酰胺组高(P<0.05);不同发育阶段卵泡构成比差异无显著性意义,均未见典型的次级卵泡.受损的卵细胞主要表现为体积变小、胞浆内出现空泡、核皱缩等.4冷冻组未出现典型动情周期的细胞类型.移植物自周围组织获得可见的血管供应,将卵巢组织块连接形成网络.移植物内毛细血管丰富,皮质中可见成群的原始卵泡,初级卵泡、次级卵泡所占比例低于原始卵泡,形态与正常者相似.4种冷冻方法冻存卵巢组织血清雌二醇水平较正常对照组明显降低(P<0.01),二甲基亚砜+乙二醇+蔗糖+乙酰胺组高于其他冷冻组(P<0.05).结果提示4种冷冻方法均对大鼠卵巢组织有一定程度破坏,初级卵泡的损伤大于原始卵泡.冻存复苏后,卵巢功能恢复,但并不完全,二甲基亚砜+乙二醇+蔗糖+乙酰胺组效果优于另外3组,但移植后动物没有出现规律的动情周期,提示冷冻方法尚需进一步完善.     

Menstrual cycle symptoms are associated with changes in low-grade inflammation

BACKGROUND: A close link between mood, low-grade inflammation and obesity has been demonstrated even in healthy subjects. We investigated the relationship between changes in physical and psychological symptoms and inflammatory markers during the menstrual cycle both in normal weight and in overweight women. MATERIALS AND METHODS: Eight healthy normal weight (body mass index 21.6 +/- 1.9 kg m(-2)) and seven overweight (body mass index 30 +/- 2.4 kg m(-2)) young women with normal ovarian function and with no premenstrual syndrome were assessed 15 times throughout their menstrual cycle. At each time point fasting blood was drawn and symptoms were recorded using the Freeman Daily Symptom Record. RESULTS: Independent of weight status, the serum concentrations of highly sensitive C-reactive protein (hs-CRP) and the total scores, in addition to the individual four factors (mood, behaviour, pain and physical symptoms), of the Daily Symptom Record varied significantly during the menstrual cycle (all P < or = 0.04) and paralleled each other. During the menstrual cycle, repeated hs-CRP serum concentrations correlated to the corresponding total symptom score and the factors mood, behaviour and physical symptoms, independent of both weight status and changes in circulating gonadal steroids (all P < or = 0.04). These associations were not observed for tumour necrosis factor-alpha serum levels. The mean hs-CRP concentrations were associated with the mean total symptom score, independent of weight status (r = 0.56, P = 0.04). CONCLUSION: Healthy young women showed psychological and physical symptoms during the menstrual cycle which changed in association with alterations in low-grade inflammation and which were independent of body weight or plasma levels of gonadal steroids.     

女性运动员不同月经周期性激素水平与运动成绩的变化

背景女性运动员月经周期与运动能力的关系,尤其是不同月经周期影响运动成绩的机制是一个值得探索的问题.目的比较不同月经周期性激素水平和运动成绩的变化情况,及对运动成绩的影响.设计单一样本单因素分析.单位浙江体育科学研究所和浙江大学教育学院及浙江省人民医院妇产科.对象在2000年对浙江省赛艇、田径队的所有女运动员中发放专门设计的月经卡,连续记录3个月以了解女运动员的月经周期状况,取月经规则的共25名作为实验对象,其中赛艇专项的女运动员12名,平均年龄19岁,田径专项的女运动员13名,平均年龄18岁.训练年限均在3年以上,每周训练6天,4～6 h/d,身体健康,月经周期规则,未使用过影响月经周期的药物.方法分别在实验对象卵泡期(周期第6～10天)和黄体期(周期第19～23天)上午800-900时抽取血样,应用全自动化学发光免疫仪测定血清雌二醇、促卵泡激素、促黄体生成素、孕酮和睾酮的含量,试剂批内变异系数＜5%.受试者并在抽血后一两天内完成运动成绩和血乳酸的测试.赛艇运动员使用美国产划船测功仪进行2 000 m、500 m全力拉,田径运动员进行100m、200m全速跑,记录所需时间;赛艇运动员在测功仪上用26次/min浆频完成2 000m的运动,田径运动员以90%的强度完成3个300 m跑,间隙3 min,运动结束后即刻取指血测定血乳酸值,采用小样本研究中配对t检验进行显著性检验.主要观察指标血清雌二醇、促卵泡激素、促黄体生成素、孕酮和血乳酸含量,赛艇运动员测功仪2 000 m、500 m成绩,田径运动员100m、200m成绩. 结果25名运动员完成全部指标检测,均进入结果分析.赛艇运动员黄体期促卵泡激素、孕酮及睾酮水平均高于卵泡期,雌二醇和促黄体生成素的水平无差异;田径运动员黄体期孕酮及睾酮的水平高于卵泡期,而雌二醇、促卵泡激素和促黄体生成素则无差别.赛艇运动员500m测功仪和田径运动员100m、200 m全力跑的成绩,黄体期均优于卵泡期,而赛艇运动员2 000 m成绩无显著性差异.运动员在卵泡期和黄体期完成同等负荷的运动时,黄体期血乳酸值均显著低于卵泡期.结论黄体期受试运动员机能状态较好,可能与月经周期中激素水平的变化以及在运动状态下各种激素间具有的协同效应有关,提示对女运动员的生理周期应引起充分的重视.     

Psychophysically determined thresholds for thermal perception and pain perception in healthy women across the menstrual cycle

OBJECTIVES: Several studies have indicated changes in sensation and/or pain sensitivity among women across the menstrual cycle, but the pattern of the changes varies considerably. One reason for the reported discrepancies could reside in lack of biochemical definition of menstrual cycle phase. The aim was to quantify temperature and temperature pain thresholds at biochemically defined stages of the menstrual cycle. METHODS: Nineteen healthy women were included in the study. Temperature and temperature pain thresholds were evaluated by quantitative sensory testing, performed at 3 occasions during the menstrual cycle (early follicular phase, late follicular phase, and mid-luteal phase). At each test session, serum concentrations of estradiol and progesterone were assessed. RESULTS: Thermal cold perception threshold at the mammilla was significantly lower in the late follicular and mid-luteal phases, compared with the early follicular phase (P<0.05, respectively). For the remaining test sites, no cycle related differences in thermal perception or thermal thresholds could be documented. CONCLUSIONS: The present study has indicated no major changes in thermal pain thresholds related to phase of the menstrual cycle for the tested locations, although thermal cold perception threshold at the mammilla was a significantly lower in the late follicular and mid-luteal phases, compared with the early follicular phase. The findings of the present study further underlines the need for strict criteria for menstrual cycle phase when studying pain sensitivity in relation to hormonal events in women.     

递增负荷运动及恢复过程中大鼠雌激素受体mRNA表达的变化及其意义

目的：观察持续9周递增负荷运动的不同训练周期及恢复过程大鼠HPO轴各环节ER mRNA水平的变化，探讨持续运动应激致动情周期紊乱ER水平变化的机制。方法：对不同训练周期及恢复过程的大鼠，采用RT—PCR检测下丘脑、垂体、卵巢、子宫各环节ER mRNA的表达，采用液相平衡竞争放射免疫分析法检测其血清雌二醇水平。结果：训练7周结束时，垂体及下丘脑ER mRNA表达及血清B水平均显著降低，卵巢及子宫ER mRNA表达则显著增加；HPO轴各环节ER mRNA表达的变化持续至恢复2期，血清E2水平持续至恢复3期基本复原。结论：递增负荷运动大鼠HPO轴各环节ER mRNA表达对运动负荷的反应不尽相同，ER mRNA表达的变化与血清E2水平密切相关．其表达水平的非同一性可能是运动性月经失调病理过程的重要一环。     

GI symptoms, function, and psychophysiological arousal in dysmenorrheic women

In a previous study, gastrointestinal (GI) symptoms and stool characteristics were found to be influenced by menstrual cycle phase. The current study was designed to replicate initial work regarding GI symptoms and stool characteristics and to explore the relationships between GI symptoms, stool characteristics, ovarian hormone levels, and indicators of psychophysiological arousal. Fifty women (dysmenorrheic, N = 22; nondysmenorrheic, N = 28) maintained a daily dairy for recording stool characteristics, GI symptoms, and daily anxiety level. First morning void urine specimens for catecholamine assays and serum samples for ovarian hormone and cortisol determinations were obtained at menses, follicular, and luteal phases. Results showed menstrual cycle-related differences in stool characteristics and report of GI symptoms, with the greatest changes occurring at menses in the total sample. Dysmenorrheic women reported higher levels of all GI symptoms at menses as compared to nondysmenorrheic women. In addition, cycle-related differences in serum cortisol, urine catecholamines, and report of anxiety were also noted, particularly in dysmenorrheic women. However, looser stools and GI symptoms at menses were not correlated with absolute levels of ovarian hormones or indicators of psychophysiological arousal.     

Assessment of the diagnostic value of myocardial scintigraphy with 201Tl chloride in women of various ages

Myocardial scintigraphy with the use of 201-thallium was performed in 92 female patients aged 38 to 87 years (initially and after 24 hours). The menstrual cycle phases were revealed to influence perfusion and metabolism of the myocardium in women with the normal ovarian cycle which should be taken into consideration in the interpretation of scintigrams in young women. The results of examining elderly persons with a long menopause have demonstrated excess saturation of scintigrams, both initial and long-term, which is primarily determined by changes in the coronary bed by the three-vascular injury type and hypertrophy of the left ventricle myocardium.     

B超对38例不孕症妇女卵泡观察

国内目前对不孕症女患者之卵泡变化报道甚少,我们对38例不孕症女患者进行63个排卵月经周期观察。观察项目为①卵巢在月经周期中的变化;②卵泡优劣、数目及大小。并根据卵泡大小、形态分为大泡型;小颗粒型;厚壁型;不均质型。并对有无正常自然排卵及B超排卵期排卵提供了可靠的时间依据,对研究不孕症女患者卵泡的病理学提供了信息。     

Light exposure, melatonin secretion, and menstrual cycle parameters: an integrative review

Dysfunction in menstrual physiology has pronounced effects on quality of life, involving mood changes, body image, infertility, and pregnancy complications. Light exposure may affect menstrual cycles and symptoms through the influence of melatonin secretion. The purpose of this systematic review is to determine the current state of knowledge about the effects of light and melatonin secretion on menstrual phase and cycle alterations. A brief overview of the influence of melatonin on human physiology is included. There is evidence of a relationship between light exposure and melatonin secretion and irregular menstrual cycles, menstrual cycle symptoms, and disordered ovarian function. In women with a psychopathology such as bipolar disorder or an endocrinopathy such as polycystic ovary syndrome, there seems to be greater vulnerability to the influence of light-dark exposure. Research on the complex role of light-dark exposure in menstrual physiology has implications for treatment of menstrual-associated disorders.     

Effects of estrogen variation on neural correlates of emotional response inhibition

Despite behavioral evidence that variation in ovarian hormones is associated with changes in affect, the neural basis of these processes is poorly understood. We combined functional magnetic resonance imaging (fMRI) with quantitative analysis of ovarian hormones in a within-subject design to investigate brain activation patterns during affective response inhibition, comparing activation between the early follicular (low estrogen and progesterone) and mid-luteal (high estrogen and progesterone) phases of the menstrual cycle in healthy women. There was significantly increased activation in the anterior cingulate and dorsolateral prefrontal cortex (DLPFC) while inhibiting response to positive words during the luteal, compared to the follicular phase. Furthermore, luteal phase estradiol level positively correlated with DLPFC activation while inhibiting response to positive words and negatively correlated with activation in several structures while inhibiting response to negative words, supporting estrogen's modulation of affective processing.