Clinical significance of the expression of inducible nitric oxide synthase in non-small cell lung cancer

Objective： To study the expressive characteristics of iNOS to the prognosis NSCLC patients. Methods： The expression in non-small cell lung carcinoma （NSCLC） and it＇s affection of iNOS was detected in 50 NSCLC tissues by Immunohistochemistry technology. Results： There was positive expression in NSCLC （positive rate 38.00%）. The expression of iNOS had a correlation with lymph node metastasis （P〈0.05）. There was no significant difference between positive expression of iNOS and lung cancer histological types, TNM stages, T stages （P〉0.05）. But the data suggested that there was a direct correlation between expression of iNOS and clinical prognosis （P〈0.05）. Conclusion： Positive expression of iNOS was observed in NSCLC, which correlated with postoperative survival time and lymph node metastasis. Although iNOS level had no significant relation with T stage, we find the positive rate in T2 stage was much higher than that in TI stage. The result suggested that positive expression of iNOS was related to bad clinical prognosis because of promoting tumor growth and metastasis.     

Detection of Brk expression in non-small cell lung cancer: clinicopathological relevance

Breast tumor kinase (Brk), also known as protein tyrosine kinase 6, is a nonreceptor tyrosine kinase containing SH3, SH2, and tyrosine kinase catalytic domains. Brk upregulation and oncogenic properties have been found in several malignant tumors, including breast, colon carcinomas, and melanomas, but the expression of Brk and its clinical significance in non-small cell lung cancer (NSCLC) remains unclear. In the current study, we examined the expression of Brk and its correlation with clinicopathological features involving p53, ki67, and E-cadherin status in NSCLC tissue using immunohistochemistry. We also used immunocytochemistry and immunofluorescent staining to examine the Brk expression and its subcellular localization in NSCLC cell lines, including LTE and H460. We further confirmed cytoplasmic and nucleus expression of Brk in LTE and H460 cells using Western blotting. The Brk expression in NSCLC cells was mainly found in cytoplasm (59/122, 48.4%) with some nucleus staining (17/122, 13.9%) with a total positive rate of 53.3% (65/122). Cytoplasmic Brk expression in NSCLC was higher than that in normal lung tissues (24/122, 19.7%) (P < 0.05). Increased cytoplasmic Brk expression in NSCLC was associated with large tumor size (≥3 cm), lymph node metastasis, and advanced tumor–node–metastasis (TNM) stages (III and IV) (P < 0.05). Moreover, increased cytoplasmic Brk expression was positively associated with Ki67 status in NSCLC (P < 0.05). Reduced E-cadherin expression was also found to be associated with lymph node metastasis and advanced TNM stages (III and IV) in NSCLC (P < 0.05). Brk expression was not associated with E-cadherin expression and P53 status in NSCLC (P > 0.05). The present findings indicate an increase of cytoplasmic Brk expression in NSCLC which may play a role in tumor development, including tumor expansion and lymph node metastasis in which Ki67, but not E-cadherin, and P53 status may be involved.     

NOB1 in Non-small-cell Lung Cancer: Expression Profile and Clinical Significance

Nin one binding (NOB1) gene has been reported up-regulated in several types of cancer. The aim of this study was to investigate the expression profile of NOB1 in non-small-cell lung cancer (NSCLC) and assess the clinical significance. qRT-PCR was used in the detection of NOB1 mRNA expression both in NSCLC tissue and in adjacent normal lung tissue. Western blot analysis and immunohistochemistry were used in the detection of NOB1 protein expression. The clinicopathological implications of NOB1 were analyzed statistically. It was confirmed by RT-qPCR that expression of NOB1 mRNA in NSCLC cells was higher than in human lung cells (P?<?0.05), and NOB1 mRNA was also over-expressed in NSCLC tissue when compared with adjacent tissue and normal lung tissue (P?<?0.05). Western blot analysis showed that NOB1 protein was significant increased in NSCLC cell lines compared with human lung cell line. Western blot analysis and immunohistochemistry showed that NOB1 protein was significant increased in NSCLC tissue compared with adjacent tissue and normal lung tissue (P?<?0.05). There were significant associations between NOB1 expression and TNM stage, lymph node metastasis, and histopathological grade (P?<?0.05), but not gender, age, smoke, or tumor diameter (P?>?0.05). Our results suggest that enhanced expression of NOB1 gene plays an important role in the occurrence and development of NSCLC. NOB1 may be a potential therapeutic target in NSCLC.     

非小细胞肺癌XIAP和Smac的表达与临床病理特征及预后的关系

  目的   探讨XIAP（X-linked inhibitor of apoptosis protein,XIAP）和Smac（second mitochondria-derived activator of caspase,Smac）在非小细胞肺癌（non-small cell lung cancer,NSCLC）组织中的表达与临床病理特征及预后的关系。   方法   采用免疫组织化学法检测70例非小细胞肺癌组织及70例对应癌旁肺组织中XIAP、Smac的表达。   结果   XIAP在70例NSCLC组织中有59例阳性表达,其中高表达16例;对应70例癌旁肺组织中有52例表达,其中高表达5例,两组XIAP表达强度比较差异有统计学意义（Z=-4.049,P < 0.001）;Smac在70例肺癌组织中有63例阳性表达,其中高（强阳性）表达32例;对应70例癌旁肺组织有53例表达,其中高（强阳性）表达5例,两组Smac表达强度比较差异有统计学意义（Z=-5.484,P < 0.001）。NSCLC组织中XIAP、Smac的表达与患者的性别、年龄、肿瘤大小、组织类型、分化程度、吸烟与否等无明显关系（P <0.05）;但二者的表达均与临床分期、淋巴结转移与否有关系（P < 0.05）。通过Kaplan-Meier法分析得出,XIAP和Smac在NSCLC中的表达与患者的预后均无明显关系（P <0.05）。   结论   1）XIAP和Smac在非小细胞肺癌组织及其对应癌旁肺组织中均有表达,但存在表达量的差异。2）XIAP和Smac在非小细胞肺癌中的表达与患者的预后均无显著关系。      

P53、C-erbB-2和VEGF在非小细胞肺癌中的表达及其临床意义

Objective: To investigate the expressions and the clinical significance of P53, C-erbB-2 and vascular endothelial growth factor(VEGF)in non-small cell lung cancer(NSCLC).Methods: 121 specimens of NSCLC were examined for P53, C-erbB-2 and VEGF by immunohistochemical staining.Results: The positive rates of P53, C-erbB-2 and VEGF in the carcinomatous tissue were 43%, 39% and 31% respectively.P53 gene protein expression in lung cancer was significantly related to histological type and P-TNM staging of lung cancer patients(P＜0.05), and was not associated with the sex, age, the size of primary cancer, lymph node metastasis and cell differentiation(P＞0.05).C-erbB-2 gene protein expression in lung cancer was closely related to histological type and cell differentiation(P＜0.05), and was not associated with the sex, age, the size of primary cancer, lymph node metastasis and P-TNM staging of lung cancer patients(P＞0.05).VEGF in lung cancer was only closely related to cell differentiation(P＜0.05), and was not associated with the sex, age, the size of primary cancer, lymph node metastasis, histological type and P-TNM staging of lung cancer patients(P＞0.05).Conclusion: It is possible for P53, C-erbB-2 and VEGF to play an important role in the oncogenesis and development of non-small cell lung cancer.     

食管鳞癌组织中Livin和Smac表达的意义

目的探讨食管鳞癌组织中凋亡抑制蛋白Livin和促凋亡基因Smac蛋白的表达及其与食管鳞癌临床生物学指标的关系。方法应用免疫组化SP法对62例食管鳞癌组织、31例癌旁不典型增生组织及62例正常食管黏膜组织进行Livin和Smac蛋白的检测,分析其阳性表达与食管鳞癌患者临床病理因素的关系。结果62例食管鳞癌组织中,组织学分级Ⅰ级15例、Ⅱ级25例、Ⅲ级22例;有淋巴结转移20例;肿瘤浸润至黏膜层、黏膜下层或浅肌层7例、深肌层14例、纤维膜41例。Livin蛋白在正常食管黏膜组织中低表达,在癌旁不典型增生组织及食管鳞癌组织中高表达,阳性率分别为38.7%（24/62）、61.3%（19/31）和80.6%（50/62）,差异有统计学意义（χ2=22.742,P<0.05）;Smac蛋白在癌旁不典型增生组织中高表达,在正常食管黏膜组织及食管鳞癌组织中低表达,阳性率分别为67.7%（21/31）、48.4%（30/62）和33.9%（21/62）,差异也有统计学意义（χ2=9.688,P<0.05）。食管鳞癌患者癌组织中Livin和Smac蛋白的表达与性别、年龄无关;与组织学分级、浸润深度及有无淋巴结转移有关（P<0.05）。Livin和Smac蛋白在食管鳞癌组织中的表达呈负相关（r=－0.426,P<0.05）。结论Livin和Smac在食管鳞癌组织中呈现异常表达,提示两者参与了食管鳞癌的发生发展和浸润转移。     

Expression of p114RhoGEF predicts lymph node metastasis and poor survival of squamous-cell lung carcinoma patients

The Rho-specific guanine nucleotide exchanging factor p114RhoGEF is involved in RhoA activation and cell motility. Previous studies suggest that altered expression of p114RhoGEF could contribute to cancer progression. We investigated an association of p114RhoGEF expression with progression and prognosis of non-small cell lung cancer (NSCLC). Immunohistochemistry was performed to detect p114RhoGEF expression in 105 NSCLC (34 adenocarcinoma and 71 squamous-cell carcinoma) and 32 normal lung tissues. We found that p114RhoGEF expression was upregulated in squamous-cell lung carcinoma and that p114RhoGEF expression was significantly higher in squamous-cell carcinoma than in adenocarcinoma or normal tissues (P?<?0.05, both). Expression of p114RhoGEF protein was significantly associated with lymph node metastasis of lung cancer (P?<?0.05), but not with patients’ age, gender, tumor size, differentiation, or stage. Expression of p114RhoGEF protein was also associated with poor overall and event-free survival of squamous-cell lung carcinoma patients (P?<?0.05). Taken together, p114RhoGEF expression may be useful in predicting progression and survival of squamous-cell lung carcinoma patients. A future study will investigate whether p114RhoGEF can serve as a novel therapeutic target in squamous-cell lung cancer.     

Prognostic significance of Livin expression in nasopharyngeal carcinoma after radiotherapy

PurposeThis study was designed to investigate the expression levels of the inhibitor of apoptosis protein Livin in nasopharyngeal cancer tissues and its prognostic significance in nasopharyngeal carcinoma after radiotherapy.Material and methodsA total of 83 patients with nasopharyngeal carcinoma who received radiotherapy were enrolled in this study from January 2008 to October 2010. Livin expression in nasopharynx pathological specimens extracted from patients was detected by immunohistochemistry. A Kaplan-Meier analysis was conducted to explore the effects of clinicopathological features and Livin expression on the overall survival and progression-free survival of patients with nasopharyngeal carcinoma, and explore its prognosis relevance after radiotherapy.ResultsOf the 83 patients with nasopharyngeal carcinoma, the overall Livin positive expression rate was 65.1% (54 patients), and the overall response rate of radiotherapy was 81.9% (68 patients). Significant differences in radiotherapy efficacy were found between patients who did not express Livin and those who did (P < 0.05). The Kaplan-Meier analysis showed that Livin expression, high clinical staging, cervical lymph node metastasis, high T-staging and high N-staging were significantly correlated with a decrease in the overall survival of patients with nasopharyngeal carcinoma (all P < 0.05). A Cox multivariate survival analysis showed that Livin expression, clinical staging and N-staging were independent risk factors for the overall survival of patients with nasopharyngeal carcinoma treated with radiation (all P < 0.05). Furthermore, Livin expression and clinical staging were independent risk factors for the progression-free survival of patients with nasopharyngeal carcinoma once radiotherapy was introduced (all P < 0.05).ConclusionExpression of Livin, an inhibitor of apoptosis proteins, may be closely linked with poor prognosis of nasopharyngeal carcinoma post-radiotherapy and hence it may be a new therapeutic target in the treatment of the disease.     

FHIT和Livin在非小细胞肺癌中的表达及临床意义

目的 检测FHIT和Livin蛋白在非小细胞肺癌组织中的表达,并对其表达水平与NSCLC临床特征之间的关系进行相关性分析,探讨其与NSCLC的浸润、转移的关系及临床意义。方法 采用免疫组织化学SP法检测60例NSCLC组织及19例癌旁正常肺组织中FHIT和Livin蛋白的表达情况,并结合肺癌的临床病理特征进行对比分析。结果 FHIT在NSCLC中的阳性表达率显著低于正常肺组织(P＜0.05),Livin在NSCLC中的阳性表达率显著高于正常肺组织(P＜0.05);FHIT和Livin蛋白表达率均与肺癌的病理类型、细胞分化程度、淋巴结转移、TNM分期密切相关(P＜0.05),而与患者年龄、性别、肿瘤部位及肿块大小无关(P＞0.05);FHIT与Livin蛋白表达两者呈负相关(P＜0.05)。结论 FHIT和Livin蛋白可以作为判断非小细胞肺癌的特异性指标,并为肿瘤基因治疗提供了新的靶点。     

EphB4、HIF-1α在肺癌组织中的表达及其意义

Objective： To investigate the relationship between the expression of EphB4 and HIF-la in lung cancer and their biological significance. Methods： The expression of EphB4 and HIF-1α was detected in 54 specimens of lung cancer by using streptavidin-peroxidase （SP）immunohistochemical method, and 10 normal lung tissues as control. Results： Among the 54 cases of lung cancer, the positive rate of EphB4 was 50.0%; 42.6% of the tumors were positive for HIF-1α. The expression of these two kinds of proteins was related to gross types, differentiation and clinical stages （P〈0.05）, but not to histological classification, age, sex and lymphoid metastasis （P〉0.05）. A highly positive correlation was observed between the expression of EphB4 and HIF-1α （P〈0.01）. Conclusion： Overexpression of EphB4 and HIF-1α may play an important role in the pathogenesis, progression and malignant degree of lung cancer. Detection of EphB4 and HIF-1α expression might be helpful to predict prognosis of patients with lung cancer.     

Expression of GPC3 protein and its significance in lung squamous cell carcinoma

The purpose of this study was to investigate GPC3 gene expression in lung squamous cell carcinoma tissue and its correlation with clinical and tumor characteristics. Using RT–PCR, the presence of GPC3 gene expression was detected in cancer tissue and adjacent normal tissue in 66 cases of lung squamous cell carcinoma and positive rates were calculated. Using Western blot, changes in GPC3 protein expression were detected in lung squamous cell carcinoma and adjacent normal tissues. The percentage of tissue samples expressing GPC3 mRNA was significantly higher in lung squamous cell carcinoma than in adjacent normal tissue (P < 0.05). This percentage was also significantly higher for cases with lymph node metastasis than for those without lymph node metastasis (P < 0.05). Further, the percentage of samples expressing GPC3 mRNA was higher with lowering degrees of tumor differentiation (P < 0.05). Rates of GPC3 expression were, however, independent of patient gender, age, and tumor size (P > 0.05). The expression of GPC3 protein in lung squamous cell carcinoma was significantly higher than that in adjacent normal tissues (P < 0.05). The expression in cases with lymph node metastasis was significantly higher than in those without lymph node metastasis (P < 0.05), and GPC3 protein expression increased with lowering degrees of tumor differentiation (P < 0.05). Further investigation is warranted for the association of initiation, development, invasion, and metastasis of disease.     

Prognostic value of Smac expression in rectal cancer patients treated with neoadjuvant therapy

The objective was to evaluate expression of second mitochondria-derived activator of caspase (Smac) expression before and after treatment in patients treated with preoperative chemoradiotherapy (CRT) for locally advanced rectal cancer and to correlate the clinicopathological characteristics and level of Smac expression with pathologic response and outcome. Expression of biomarker was evaluated by immunohistochemistry in tumor samples from 98 patients with clinical Stage II and III rectal cancer treated with preoperative pelvic radiotherapy plus concurrent chemotherapy. All patients received a standardized total mesorectal excision procedure after a long interval of 4–6 weeks. For Smac, patients with a good response to neoadjuvant CRT tended to have higher pre-therapy levels (P = 0.007). The level of Smac expression decreased after neoadjuvant therapy (P = 0.016). High expression of Smac before CRT, and high Dworak’s tumor regression grade (TRG) were significantly associated with improved 5-year disease-free survival (P < 0.05). Pretreatment nodal status also was significantly associated with 5-year disease-free survival and 5-year local relapse-free survival (P < 0.05). Multivariate analysis confirmed that the pretreatment expression of Smac and Lymph nodal status were independent prognostic factors. Our study suggests that high expression of Smac before neoadjuvant CRT could predict good outcome in locally advanced rectal cancer patients.     

AKT1表达与非小细胞肺癌临床病理特征及预后的关系

目的探讨丝/苏氨酸蛋白激酶1(AKT1)表达与非小细胞肺癌(NSCLC)病理学特征及预后的关系。方法选取经病理学检查证实的NSCLC患者90例,分别取其癌组织、癌旁组织为NSCLC组和癌旁组,采用免疫组化染色法检测2组标本中的AKT1蛋白表达水平,并分析AKT1蛋白与NSCLC患者TNM分期、淋巴结转移情况、肿瘤分化程度、病灶的大小及预后等指标的关系。结果NSCLC组标本中的AKT1蛋白阳性表达率(58.89%)显著高于癌旁组(11.11%),差异具有统计学意义(P<0.05)。高分化及中分化、TNM分期(Ⅰ期+Ⅱ期)、未发生淋巴结转移的NSCLC组中AKT1蛋白阳性表达率低于低分化、TNM分期(Ⅲ期和Ⅳ期)及发生淋巴结发生转移NSCLC组织(P<0.05),不同年龄、性别、病灶大小及病理学类型的NSCLC组织中AKT1蛋白阳性表达率差异无统计学意义(P>0.05)。NSCLC组标本中的AKT1蛋白阳性表达与阴性表达患者的1年、2年生存率差异无统计学意义(P>0.05),AKT1蛋白阳性表达患者的3年生存率(13.21%)显著低于AKT1蛋白阴性表达患者的37.84%(P<0.05)。结论NSCLC患者癌组织中的AKT1蛋白表达上调,并且与肿瘤进展及不良预后关系密切。     

Livin和Smac蛋白在非霍奇金淋巴瘤中的表达及其临床意义

 目的　研究抗凋亡蛋白Livin和促凋亡因子Smac在非霍奇金淋巴瘤（NHL）中的表达及其与临床特征和预后间的关系。方法　应用免疫组织化学方法检测31例NHL患者Livin和Smac表达情况,并将其与临床随访进行临床特征和生存预后关系的研究。 结果　Livin蛋白高表达的患者多为有B症状、Ⅲ／Ⅳ期和国际预后指数（IPI）高危者,而Smac蛋白高表达患者多为无症状、Ⅰ／Ⅱ期和IPI低危者。但Livin和Smac蛋白表达均与性别和年龄无相关关系。Livin和Smac两者之间无相关关系（r＝0.003,P＞0.05）。Livin蛋白低表达患者的生存情况明显好于高表达患者,而Smac蛋白高表达的生存情况明显好于低表达患者。结论　Livin表达与NHL不良临床特征及预后相关,而Smac表达与良好的临床特征及预后有关。     

Down-regulation of lysyl oxidase-like 2 (LOXL2) is associated with disease progression in lung adenocarcinomas

Lysyl oxidase-like 2 (LOXL2) belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. Both down- and up-regulation of LOXL in tumor tissues and cancer cell lines have been described, suggesting paradoxical roles in cancer. However, LOXL2 expression and the clinical significance in non-small cell lung cancers (NSCLC) remain unresolved. Real-time PCR was performed to detect the expression of LOXL2 mRNA in lung tumor tissues (TT) and surrounding normal tissues (sNT). Moreover, the expression of the LOXL2 protein in specimens from 83 paraffin-embedded blocks was examined by immunohistochemical staining. Correlations between LOXL2 mRNA and protein expression and clinicopathological features were evaluated by statistical analysis. In the 137 patients examined, LOXL2 mRNA expression was significantly lower in lung TT than the sNT (P?<?0.05). Forty-eight specimens (48/83) showed low expression of LOXL2, as characterized by immunohistochemical staining. By statistical analysis of the correlation between LOXL2 mRNA expression and clinical features of NSCLC patients, down-regulation of Loxl-2 mRNA expression was correlated with male patients (P?=?0.008), a poorer N-stage (P?=?0.032) and a poorer pathological TNM stage (P?=?0.003). Statistical analysis of the correlation between LOXL2 protein expression and clinical features of NSCLC patients showed a statistically significant difference between low expression of the LOXL2 protein and a poorer N-stage (P?=?0.036), a higher pathological TNM stage (P?=?0.005) and poorer differentiation (P?=?0.035). When stratified by histological types, significant differences at both the mRNA and protein levels were only found for lung adenocarcinomas patients, and not for lung squamous cell carcinomas patients. The level of LOXL2 mRNA expression was found to be significantly down-regulated in NSCLC, and the lower mRNA and protein expression levels correlated with poorer differentiation, higher N-stage and advanced pathologic TNM stage in patients with lung adenocarcinomas.     

Smac在Ⅰ～Ⅱ期非小细胞肺癌组织中的表达及其临床意义

背景与目的：Smac是新近发现的一种由线粒体释放的促凋亡蛋白,主要作用于凋亡抑制蛋白（inhibitor of apoptosis proteins）．促进凋亡的发生：Smac在体外实验中显示出抗肿瘤作用,但在肺癌中其作用尚不明确。本研究通过检测Smac蛋白在Ⅰ～Ⅱ期非小细胞肺癌的表达,探讨其与临床病理因素及预后的关系。方法：采用组织芯片和免疫组化方法,对213例Ⅰ、Ⅱ期非小细胞肺癌肿瘤组织中Smac蛋白的表达情况进行检测。结果：Ⅰ、Ⅱ期NSCLC的5年生存率分别为61．9％和30．0％。Smac蛋白主要定位于细胞浆,以75％的阳性细胞数为分界点时,低表达组占60．6％。高表达组占39．4％。Smac的表达与性别、年龄、血型、病理类型及生存无相关性（P〉0．05）,但Smac蛋白在无淋巴结转移和有淋巴结转移的表达分别为37．0％和58．3％．二者比较有统计学意义（P〈0．05）。结论：Smac蛋白与Ⅰ～Ⅱ期非小细胞肺癌的淋巴结转移有关。     

Clinical and Imaging Features of Non–Small-Cell Lung Cancer in Young Patients

BackgroundNon–small-cell lung cancer (NSCLC) in young adult patients is rare, with scarce data available in patients aged < 40 years and even less in those aged < 35 years. Our goal was to determine the presenting symptoms, clinicopathologic characteristics, and imaging features of young patients with NSCLC at time of diagnosis and compare them to those of older adults.Patients and MethodsWe retrospectively analyzed the medical records and imaging of young patients (≤ 40 years old) with NSCLC treated at our institution between 1998 and 2018. Patients < 35 years old were compared to those between 35 and 40 years old. Characteristics of patients ≤ 40 years old were compared to older patients (> 40 years) from publicly available data sets.ResultsWe identified 166 young patients with NSCLC (median age, 36.6 years; range, 18-40 years). Most presented with nonspecific respiratory symptoms and were diagnosed with pneumonia (84/136, 62%). Compared to patients < 35 years old, patients 35-40 years old were more likely to have malignancy detected incidentally (15% vs. 5%, P = .04). Patients < 35 years old were more likely to have central tumors (55% vs. 33%, P = .02) and to have bone (38% vs. 19%, P = .007) and lung (39% vs. 24%, P = .03) metastases. Compared to older patients (> 40 years), young patients were more likely to be never smokers (65.0% vs. 14.7%, P < .001) and to have advanced disease (88% vs. 66%, P < .001).ConclusionYoung patients with NSCLC often present with nonspecific symptoms and have advanced disease at diagnosis, often mimicking other pathologies. Awareness of the clinical presentation and imaging features of NSCLC in young patients may help minimize delays in diagnoses.     

非小细胞肺癌中上皮钙粘附素的表达及其意义

目的研究上皮钙粘附素(epithelialcadherin,E-cad)在非小细胞肺癌(non-smallcelllungcancer,NSCLC)中的表达,探讨E-cad与NSCLC的组织类型、病理分级、TNM分期及淋巴结转移的关系。方法应用免疫组织化学SABC法检测65例原发性NSCLC,20例癌旁组织中E-cad蛋白的表达水平。结果20例正常肺组织均阳性表达,且均匀分布于膜表面。NSCLC中E-cad表达不均匀,阳性率为32.3%,与正常组比较差异有显著性(P<0.05)。但与组织类型无关(P>0.05),与病理分级、TNM分期及淋巴结转移显著相关(P<0.05)。结论E-cad与NSCLC的发生发展呈显著负相关,低表达者预示预后不良。     

Expression of MMP-13 and its correlation with prognosis in non-small cell-lung cancer

Objective： To investigate the expression of MMP-13 in non-small cell lung cancer （NSCLC）, so as to analyze its correlation with prognosis of NSCLC. Methods： MMP-13 expression was detected in 99 NSCLC tissues and 32 normal lung tissues by immunohistochemical method. Results： （1） Expression of MMP-13 （51.5%, 51/99） in cancer tissues was significantly higher than that in normal tissues 0% （P 〈 0.05）. Expression level of MMP-13 was significantly related to lymph node metastasis and clinical stage （P 〈 0.01）. （2） Kaplan-Meier analysis showed that expression level of MMP-13 was closely cor- relate with the prognosis of NSCLC. Multivariate Cox model analysis suggested that the survival time was significantly related to clinical stage and the expression of MMP-13. Conclusion： MMP-13 is an independent factor that affect prognosis.