克山病病区糖饲养豚鼠的冠状动脉平滑肌细胞膜电位的研究

本文应用细胞内微电极方法，研究了克山病病区粮喂饲料喂饲豚鼠的冠脉平滑肌细胞膜电位变化，取得如下结果：（１）实验动物冠脉平滑肌细胞静息膜电位为－４９．８±５．３ｍＶ，对照组动物为－４９．９８±６．２ｍＶ（Ｐ＞０．０５）。（２）硒可使实验组动物冠脉平滑肌细胞膜电位去极化。其程度明显小于对照动物。（３）氯化钾和乙酰胆碱可分别引起实验动物冠脉平滑肌细胞膜电去极化和超极化，且较对照动物的变化明显增大。这些结     

缺硒大鼠心肌α_1受体、cAMP及儿茶酚胺含量的变化

实验用SD大鼠随机分为三组,分别用克山病区低硒粮饲料(Se 0.010ppm)、病区粮加亚硒酸钠饲料(Se 0.181ppm)及本院常规饲料喂养两月,处死动物,测定各项指标。结果发现,常规饲料组大鼠心肌与     

钼对大鼠心室肌细胞动作电位的影响

用克山病区粮(低Se)、非病区粮加W、病区粮加Mo喂养大鼠,分别复制低Se、低Mo及低Se高Mo的动物模型。病区粮组血Se浓度低于对照组(P<0.001);非病区粮加W组血Mo浓度低于对照组(P<0.001);病区粮加Mo组血Se浓度低于对照组(P<0.001)而血Mo浓度高于对照组(P<0.001)。采用浮置微电极技术,观察各组动物在体心肌细胞动作电位变化,结果显示;低Se与低Mo组APA均降低,且低Se加Mo组APA恢复正常,并使APD_(90)延长,提示Mo与Se对心肌细胞膜有类似功能。     

灯盏花素对大鼠心室肌细胞钠通道的影响

目的： 研究灯盏花素对大鼠心室肌细胞膜钠通道的影响,在离子通道水平探讨灯盏花素的抗心律失常作用机制。方法： 用急性酶解法获得单个大鼠心室肌细胞,标准的全细胞膜片钳技术记录钠通道电流(INa)。结果： (1) 灯盏花素呈浓度依赖性抑制INa,在-30 mV时,含1、3、30、100 mg·L-1灯盏花素的细胞外液分别灌流细胞3 min,分别阻断INa峰电流(7.98±0.60)%、(37.73±2.31)%、(65.58±2.90)% 和(88.09±5.60)%。INa激活电位为-70 mV,最大峰电位为-30 mV,翻转电位为5 mV,激活和失活过程呈电压和时间依赖性。30 mg·L-1灯盏花素使电流电压曲线明显上移,峰值电流从（13.49±1.25）pA/pF 减少至（4.78±0.85）pA/pF,n=8,P<0.05,冲洗后可以不完全恢复。（2）灯盏花素能使钠电流失活曲线明显左移。（3）灯盏花素使钠电流激活曲线明显右移。（4）灯盏花素使钠电流复活明显减慢。结论： 灯盏花素能够抑制心肌细胞钠通道电流,并呈浓度依赖性。     

豚鼠结肠平滑肌细胞L型钙通道特性研究

目的　全细胞式膜片钳技术研究豚鼠结肠平滑肌细胞的L型钙通道特性 ,以加深对胃肠动力调控的认识及有利于胃肠动力药物的开发。方法　豚鼠 ,2 0 0～ 4 0 0g ,木瓜蛋白酶酶消化法分离结肠纵肌细胞。全细胞膜片钳法记录单细胞的钙通道电流。刺激模式有两种 ,一种用于电流电压曲线 (IV曲线 )将细胞钳制在 - 70mV或 - 90mV ,10mV的阶跃刺激去极化从 - 80～ +6 0mV ,4 0 0ms。另一种为双脉冲模式 (two pulseprotocol) ,将细胞膜电位钳制在从 - 80～ +30mV 6S后 ,去极化到 10mV10 0ms。激活及失活曲线用BoltzmannfunctionI Imax=1 (1+exp[(V…     

克山病病区粮饲养豚鼠的冠状动脉反应性的研究

克山病以围血管的心肌坏死灶为其特征，本文研究了血管因素在其发病中的作用。所用动物模型是用豚鼠饲以克山病病区粮建立的。冠状动物的反应性的研究采用测定血管条单位平方面定积实际收缩张力的方法（ｍｇ／ｍｍ＾２）。结果：（１）Ｓｅ对模型动物冠脉平滑肌的舒张作用明是减弱，使其剂量－反应曲线右移，表明钙通道活动的加强。（２）在动物模型，激动剂ＫＣｌ及Ａｃｈ产生的实际收缩张力显著增强，表明此时冠状动脉平滑肌反应性     

实验性糖尿病大鼠心肌细胞膜完整性的改变

应用电生理和辣根过氧化物酶示踪技术观察链脲佐菌素引起的实验性糖尿病大鼠心肌细胞膜完整性的变化。实验结果表明,糖尿病大鼠心肌细胞动作电位波幅、最大舒张电位,阈电位和最大除极速度均比对照动物明显减低(P<0.001),而复极不同水平的动作电位时程比对照动物显著延长(P<0.001),糖尿病大鼠心肌中辣根过氧化物酶阳性的肌细胞数明显多于对照动物(P<0.05)。这些结果说明,糖尿病大鼠心肌细胞膜存在损伤的电位变化,并有肌膜通透性增大。提示心肌细胞膜完整性的损害在糖尿病性心肌病的发生机理中可能起一定作用。     

硒和维生素E对用克山病病区粮饲养大白鼠的机体抗氧化功能的影响

72只断乳雄鼠分成6组,分别喂以以克山病病区粮配成的不同水平的硒(0.007PPm和0.175PPm和不同水平的维生索E(0.96mg/kg和25mg/kg)的饲料,为期15周。实验结束时测定了血、心脏和肝脏中硒和维生素E含量及谷胱甘肽过氧化物酶活     

细胞内记录大鼠离体颈上神经节后神经元膜电导的实验研究

应用细胞内生物电记录技术,观测大鼠离体颈上神经节后神经元在静息状态,快兴奋性突触后电位(f-EPSP)发生期间的膜电导以及膜时间常数和膜电容.静息状态下膜电导为(16.5±7.4)nS(n=45),快兴奋性突触后电位(f-EPSP)发生期间的膜电导为(27.2±5.6)nS(n=42),后者明显高于前者(P＜0.05);膜时间常数无明显变化;膜电容也有明显差异.快兴奋性突触后电位(f-EPSP)发生期间的膜电导的增大和翻转电位值16mV的结果提示交感神经节内快兴奋传递过程是几种阳离子通道(如Gk,Gna)同时开发的净效果.本实验为研究各种物理因素(电离辐射,电磁波,激光等)对交感神经节内快兴奋传递过程的影响提供基础理论依据.     

炙甘草汤含药血清对家兔心室肌细胞瞬时外向钾电流的抑制作用

目的:探讨炙甘草汤含药血清对家兔心室肌细胞瞬时外向钾电流(Ito)及其动力学的影响。方法:用酶解法分离单个兔心室肌细胞。血清药理学法确定血清中炙甘草汤含药浓度。全细胞膜片钳技术记录炙甘草汤含药血清对兔心室肌细胞Ito的影响前后变化。结果:20%炙甘草汤含药血清作用心肌细胞5min,可使Ito明显减小。指令电位为+50mV时,可使Ito从(43.3±5.89)pA/pF减少到(7.98±2.78)pA/pF(n=6,P<0.01)。该含药血清对Ito的抑制作用可逆。在5%~40%范围内,该含药血清对Ito的抑制作用为浓度依赖性,IC50的平均值为12.18%。20%该含药血清使各膜电位水平下Ito减小,I-V曲线下移。不改变Ito稳态激活动力学曲线,但使失活动力学曲线显著左移。50%Ito失活曲线点分别为(-41.4±3.5)mV和(-79.9±2.9)mV(n=8,P<0.05)。同时使Ito失活后恢复时间延长,用药前后50%Ito恢复时间分别为(18.2±2.5)ms和(44.1±2.2)ms(n=8,P<0.01)。结论:炙甘草汤含药血清对家兔心室肌细胞Ito具有显著的抑制作用,这可能是炙甘草汤抗心律失常的可能电生理基础之一。     

Level of functioning of siblings and parents of probands of varying degrees of retardation

A further analysis of already published data supports the position that retardates of low ability level less frequently have retarded siblings, retarded parents, and parents low in occupational level than do retardates higher in ability level. The analysis supports the position that there are two types of retarded individuals, persons retarded as a result of gene or chromosomal anomalies, brain injury, etc., who more frequently occur in the lower-level retardate group, and persons whose retardation represents polygenic segregation, who more frequently occur in the higher-level group.     

局部注射辛伐他汀对骨质疏松大鼠股骨髁骨小梁的改建效应

背景：局部注射具有成骨作用的辛伐他汀,可显著增加骨质疏松大鼠股骨颈及股骨髁部的骨密度及力学强度,分析局部注射辛伐他汀对股骨髁骨小梁的影响。 目的：进一步研究骨质疏松大鼠股骨内局部注射辛伐他汀对股骨髁骨小梁的影响。为将辛伐他汀应用于临床骨质疏松局部治疗提供实验基础。 方法：18只雌性SD大鼠双侧卵巢切除后3个月,制备大鼠骨质疏松模型。实验大鼠随机数字表法均分为3组,分别在实验大鼠的右侧股骨髓腔内单次注射辛伐他汀溶液5 mg、10 mg,对照组单纯注射空白载体。分别在注射后1个月处死大鼠并取材。Micro-CT扫描并定量分析骨组织形态变化。 结果与结论：给药后1个月,Micro-CT扫描结果显示,辛伐他汀治疗组的骨微结构参数如骨皮质厚度、骨小梁密度及连接率明显优于对照组。说明疏松骨骼单次注射小剂量辛伐他汀可显著促进股骨髁部骨小梁改建,改善骨骼微结构,可为强化局部、防治骨质疏松骨折的新选择进一步提供实验基础。     

石斛属民族药用植物的分类及生药学研究

中药石斛早在《神农本草经》中就被列为上品,其应用历史悠久,具有养阴生津、补肾益气、润喉护嗓、活血明目、抗癌防老等功效。本文对国内外石斛属民族药用植物的分类及生药学研究进行了综述。     

膝关节骨性关节炎的关节镜治疗体会

目的我科自2001～2005年4月对59例67膝的骨性关节炎（0A），进行关节镜检查及镜下清理术。方法镜检：膝关节骨性关节炎伴不同程度滑膜炎，滑膜皱壁粘连纤维片，关节软骨不同程度破坏。镜下清除增生滑膜，松解粘连，去除剥脱软骨、修复关节面。结果随访2月～4年，平均1.5年，术后综合评估2月～1年，优良率94％；1～2年，优良率79.2％；2年以上优良率54.8％。结论关节镜对膝关节骨性关节炎诊断能提供了比较全面的资料，并对骨性关节炎早期有良好疗效，具有创伤小、恢复快、并发症少和重复治疗等优点。     

Anatomical study of distribution of valves of the cutaneous veins of adult''s limbs

Since the cutaneous veins of the four limbs have been used as autogenous grafts in the treatment of cardiovascular disorders, knowledge of distribution of the valves is increasingly required depending upon the use. In the gross anatomical study of distribution of valves of the trunci venae, there has been argument about locational relationships between the venous roots and the valves in the vicinity of the roots and the inter-valvular distance. However, there have been only few reports discussing detailed information about valves of the cutaneous veins of the four limbs. The authors observed patterns of distribution of the cutaneous venous valves of the four limbs of cadavers prepared for practice in anatomy. The following parts were excised from each cadaver: the cephalic, basilic, and the great saphenous veins, which originate from the acral venous network and flow into the proximal deep veins, and the venous roots communicating with these veins. An incision was made on each excised vein in the direction of the long axis under observation with a stereoscopic microscope, and the inter-valvular distance and the distance between the valve and the orifice of venous root in the vicinity of the valve were measured. The inter-valvular distance varied with type of the truncus venae, and it varied according to area even in the same truncus venae.     

Pathways of the realization of immunomodulating effects of neurotransmitters

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 115, N ^o 4, pp. 401–404, April, 1993     

Characterization of sterols of three digenetic trematodes of buffalo

Sterols of three digenetic trematodes were isolated and characterized by infrared and 1H nuclear magnetic resonance spectroscopies, and gas-liquid chromatography and gas chromatography-mass spectrometry. Sterols identified were cholesterol, cholestanol, 24-methylcholesterol, 24-methylcholestanol, 24-ethyl-22-dehydrocholesterol, 24-ethyl-22-dehydrocholestanol, 24-ethylcholesterol and 24-ethylcholestanol.     

Mechanisms of generation of slow components of bioelectric activity in diagnostics of functional state of the gastrointestinal tract

New electrophysiologic devices were used for the diagnostics of the state of excitable structures in the gastrointestinal tract and correction of their motor functions. Bioelectrical and biomechanical activities form the basis of functioning of internal organs. The mechanisms of generation of slow bioelectrical activity that are important for clinical and physiological studies are described. One of these mechanisms is a capacitance parametric transducer converting the energy of contractions into specific electric signals reflecting muscle functions. Another mechanism results from slow oscillations of resting potentials of interrelated excitable cells in large neuromuscular structures of internal organs. The elaborated procedure is efficient for preventing early postoperative paresis of the gastrointestinal tract. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 128, No. 10, pp. 448–452, October, 1999     

胃小弯侧血管弓的MDCT解剖

目的：研究正常胃小弯侧血管弓及其属支血管的多排螺旋CT表现。方法：回顾性分析51例正常成人的上腹部CT资料,观察胃小弯侧血管弓及其属支血管的显示情况、血管弓形态及其与胃壁的关系。结果：51例均显示胃小弯侧血管弓,血管弓贲门部属支、胃体部属支和幽门部属支分别显示46例、15例和10例;血管弓与胃壁紧邻41例。结论：MDCT检查可良好显示正常胃小弯侧血管弓及其属支血管。     

Assessment of the efficacy of treatment of hemangiomas

Assessment of the aftereffects of cryoexposure and ultrahigh-frequency cryoexposure on hemangioma tissue of various types, cavernous and squamous, showed a higher cryogenic effect in hemangiomatous tissue preexposed to ultrahigh-frequency waves. A quantitative criterion is proposed for assessing the efficacy of the studied methods of exposure. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 6, pp. 669–672, June, 1995