针刺足三里穴后续效应的脑功能成像研究

[目的]利用功能磁共振探讨针刺足三里穴的后续效应对脑部的影响.[方法]选择16名健康志愿者,分别在针刺足三里前及拔针后25 min对其进行磁共振功能扫描,应用局部一致性(regionall homogeneity,ReHi)方法处理fNRI数据.[结果]拔针后25 min时,右侧额中回、左侧额上回、右侧颞上回、后扣带回...     

Effect of Zusanli (ST 36) moxibustion on rat mesenteric microvascular system

ObjectiveTo investigate the effect of moxibustion on Zusanli (ST 36) on visceral-mesenteric vessels by observing circulation.MethodsForty-five SD rats were randomly assigned to a moxibustion, electroacupuncture (EA), and blank group. In the moxibustion group, heat stimulation of moxibustion to the Zusanli (ST 36) area of normal rats was performed for 15 min. In the EA group, needles were inserted into the Zusanli (ST 36) and lateral point [0.5 cm lateral from Zusanli (ST 36)] for 15 min. The blank group was not given any treatment. We continuously monitored mesenteric microvascular changes with in vivo microscopic video.ResultsMoxibustion and EA to Zusanli (ST 36) increase the diameter of mesenteric arterioles and venules (P<0.05). There were no obvious changes in the blank group. Fine arterial diameter peaked at 12 min in the moxibustion group, while it peaked at 15 min in the EA group.ConclusionsThe stimulation of moxibustion and acupuncture to Zusanli (ST 36) has immediate effects on expanding the microvasculature. This dilation may be the mechanism of the gastrointestinal effect of Zusanli (ST 36).     

针刺效应与脏腑功能失调程度的关系

探讨在相同的针刺刺激条件情况下,针刺效应与机体状态的关系.通过匀速持续静脉给予2种不同剂量的盐酸山莨菪碱,制作2种胃电异常背景,观察比较针刺足三里对2种程度不同的异常胃电状态的调节.结果:在2种胃电异常背景下,对足三里穴位施以同样的针刺刺激,针刺的效应有所不同.说明针刺效应与机体异常状态的程度有关.     

Effects of Warm Needling at Zusanli (ST 36) on NO and IL-2 Levels in the Middle-Aged and Old People

42 middle-aged and old people at the age between 55-70 years were selected and given the warm needling at Zusanli (ST 36), and their IL-2 and NO contents of peripheral blood before and after acupuncture were determined. The results showed that IL-2 and NO contents increased significantly after the warm needling (P < 0.01).     

Auricular-Plaster Therapy Plus Acupuncture at Zusanli for Postoperative Recovery of Intestinal Function

In order to relieve the abdominal distension and other discomforts due to gastrointestinal dysfunctionafter abdominal operations,the patients were treated by auricular-plaster therapy plus acupuncture atZusanli(ST 36).12(92.4%)of the 13 cases in the treatment group showed recovery of normalperistalsis within 72 hours after operations,while that in 13 cases of the control group was 46.1%,indicating that auricular-plaster therapy plus acupuncture at Zusanli(ST 36)may promotepostoperative recovery of the intestinal function.     

电针足三里穴对胃粘膜保护作用的机制研究

[目的]探讨电针足三里穴对胃粘膜的保护作用机制。[方法]将20只狗随机分为空白对照组、非经非穴组、上巨虚组、足三里组（每组5只）。采用激光多普勒血流仪连续监测狗胃粘膜血流量的变化，同步测定血浆及胃粘膜组织中降钙素基因肽（CGRP）含量，并观察其变化规律。[结果]电针足三里穴可使狗胃粘膜血流量增加（与针刺前比P＜0.05或P＜0.01），使血浆和胃体粘膜中的CGRP含量增加（P＜0.05或P＜0.01)；而针刺上巨虚穴有同样的作用趋向，但未显示统计学意义；针刺非经非穴组未见这一变化。[结论]说明电针足阳明胃经穴位，尤其电针足三里穴可以改善胃粘膜的血流量，这种改善可能与针刺影响血浆和胃粘膜的CGRP含量升高有关，这可能也是电针足三里可以对胃粘膜起保护作用的机制之一。     

《黄帝内经》中足三里穴的定位及主治功用

本文系统梳理了《黄帝内经》中涉及足三里穴的有关条文,依据经文原旨,参考注家有关注释,从腧穴名称、归经定位、取穴方法、针刺方法、主治病证等方面,对足三里穴主治功用、主治特点进行考证.     

激光针内关等穴位对家兔急性缺血性心肌损伤的影响

本实验共用家兔36只,结扎其左冠状动脉心室支造成急性心肌缺血性损伤,以心电图ST_(11)、STavF段电位变化为指标,观察激光针内关等穴位对家兔急性缺血性心肌损伤的影响。实验观察到激光针内关穴有促进急性缺血性心肌损伤恢复的作用,而激光针足三里组、非经穴组和对照组的作用则不明显,提示手厥阴心包经内关穴的作用具有相对特异性.     

Acupuncture plus Point-injection for 32 Cases of Obstinate Urticaria

In order to observe the therapeutic effects of acupuncture plus point-injection for obstinate urticaria, 64 cases of obstinate urticaria were randomly divided into the following two groups. 32 cases in the treatment group were treated with acupuncture at the points of Quchi （LI11）, Xuehai （SP10）, Zusanli （ST 36）, Sanyinjiao （SP6） and Fengchi （GB20） plus point-injection at the points of Zusanli （ST36） and Quchi （LI11）. 32 cases in the control group were treated with antihistamines （such as Acrivastine, Cinnarizine or Ranitidine）. The results showed that the therapeutic effect in the treatment group was obviously better than that in the control group （P〈0.05）, with a much lower relapse rate in the former than that in the latter （P〈0.01）.     

针刺胃经下肢段及非经非穴点对胃电的影响

目的探讨足阳明胃经对胃功能调控的相对特异性。方法静脉持续输入盐酸山莨菪碱制作胃电节律规整性下降的异常状态背景。针刺胃经穴位足三里、梁丘以及穴位旁开1．5cm的非经非穴点，比较足三里、梁丘及其穴位旁开对照点对胃电节律的调整作用。结果足三里、梁丘及其穴位旁开对照点均能对抗盐酸山莨菪碱引起的胃电节律规整性下降。足三里、梁丘穴较其旁开对照点对胃电节律的调整作用好。结论足阳明胃经下肢段对胃功能调控具有相对特异性。     

食蟹猴左足三里穴针刺fMRI研究

目的 对食蟹猴进行左侧足三里穴针刺的fMRI研究,探讨左侧足三里穴针刺所引起的脑功能活动.方法 食蟹猴6只,采用3.0T MR扫描仪,动物麻醉后进行左侧足三里穴磁共振功能成像扫描.针刺fMRI采用"静息-刺激-静息"设计模式.fMRI-BOLD扫描16层,128 phase.采用SPM2软件处理fMRI实验数据,采用t检验来分析特定任务刺激下脑激活的状态.结果 针刺左侧足三里穴可以观察到左侧中央前回、右侧中央后回、双侧岛叶、楔前叶、右侧颞叶激活;额叶、顶叶、枕叶、扣带回有负激活点.结论 可以在临床使用的MRI设备上对非人灵长类动物进行脑fMRI的研究.针刺食蟹猴左侧足三里穴,fMRI可以显示不同脑组织区域的正激活和负激活.针刺的作用机理可能与中央前回、岛叶及颞上回有较密切关系.针刺足三里穴引起的负激活定位性较差,扣带回的负激活在针刺机理及针灸止痛中可能有重要作用.     

针灸改善海洛因依赖者睡眠障碍和焦虑情绪35例

目的观察针灸对海洛因依赖者睡眠障碍和焦虑情绪的改善作用。方法将62例男性海洛因依赖者分为对照组（27例）和针灸组（35例）。针灸组针刺百会、内关、神门穴,留针30 min;同时,艾灸足三里穴,每次30 min,每周2次,共8周。对照组不接受任何治疗措施。采用焦虑自评量表（self-rating anxietyscale,SAS）和匹兹堡睡眠质量量表（Pittsburgh sleep quality index,PSQI）分别观察两组治疗前后焦虑状况和睡眠质量。结果治疗后,针灸组SAS总分和标准分、入睡时间和PSQI总分较治疗前显著减少（P〈0.05）,睡眠时间显著延长（P〈0.05）;对照组仅入睡时间和睡眠时间较治疗前有显著改善（P〈0.05）。结论针灸对海洛因依赖者睡眠障碍、焦虑情绪都具有改善作用。     

针灸足三里治疗中老年脑出血患者院内感染临床研究

目的:探讨针灸足三里治疗中老年脑出血患者院内感染的临床疗效。方法:选取80例脑出血合并院内感染的中老年患者,根据数字表法随机分为两组,试验组与对照组,对照组采用常规药物治疗,试验组在常规药物治疗的基础上给予针灸足三里治疗,对比分析两组临床治疗效果、生存质量评分情况以及格拉斯哥评分(glasgow coma scale,GCS)情况。结果:试验组患者有效率为95.00%明显高于对照组(62.50%),差异有统计学意义(χ2=15.740 4,P=0.000 38)。试验组患者的生存质量评分(32.35±11.53)分,对照组生存质量评分(62.42±16.53)分,两组比较,差异有统计学意义(t=10.008 7,P=1.724 7×10-16)。试验组治疗后患者的GCS评分(6.74±0.67)分,对照组GCS评分(5.97±0.53)分,两组比较,差异有统计学意义(t=5.700 5,P=1.018 8×10-7)。结论:针灸足三里治疗中老年脑出血患者院内感染的临床效果显著,降低院内感染发生率,提高生活质量,安全可靠。     

Differences in thermal effects of moxibustion at Zusanli(ST 36) and Hegu(LI 4) on various facial areas in healthy people

OBJECTIVE:To study the effects of temperature on different facial areas by suspended moxibustion at two points,Zusanli(ST 36) and Hegu(LI 4),and probe the phenomena underlying self-regulation in the human body after thermal stimulation.METHODS:Thirty healthy volunteers accepted moxibustion over Zusanli(ST 36) and Hegu(LI 4),and the order of moxibustion points was randomly determined.Moxibustion method:suspension of moxibustion over Zusanli(ST 36) and Hegu(LI 4) on both sides was performed using an ignited moxa stick stuck in a support for 20 min.Observation method:An infrared thermal image of the face was taken before and after suspended moxibustion using a CK350 medical infrared thermal imaging instrument.Data analysis:A thermal microscopic section view system(TMTSys) was used to analyze the change in temperature in special facial areas.Statistical analysis was carried out using SPSS 14.0 software.RESULTS:Before moxibustion was suspended,the facial thermal image showed a T-shaped thermal area related to the vascular distribution with even temperature and good symmetry on both sides.Suspended moxibustion over Zusanli(ST 36) have a very significant increase in temperature at the forehead,around the nose,at the corners of the mouth,and at the cheeks and lips(P<0.01).Suspended moxibustion over Hegu(LI 4) also have a significant(P<0.05) increase in temperature around the nose,the corners of the mouth,the cheeks,and lips,where has a new high temperature area was formed(P<0.01).Suspended moxibustion over Hegu(LI 4) raised the temperature at the middle point of the lips more obviously than did Zusanli(ST 36) in the same person,(P<0.05).After 10 min of moxibustion over Zusanli(ST 36) and Hegu(LI 4),the change in temperature in the facial area reached its peak value.CONCLUSIONS:Facial infrared thermography of healthy people revealed a T-shaped thermal area reflecting a physiological thermal area.Moxibustion over Zusanli(ST 36) or Hegu(LI 4) raised the temperature in this facial T-shaped thermal area.Hegu(LI 4) led to the formation of a new thermal area in the lips.The time required for moxibustion to regulate human body temperature was 10 min.     

壮医药线点灸联合针刺对失眠患者血浆Orexin及HPA轴的影响

目的观察失眠患者血浆Orexin、促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)含量的变化,讨论壮医药线点灸联合针刺治疗失眠的机制。方法 50例失眠患者为针灸组,54例作为健康对照组,针灸组予以壮医药线点灸足三里联合针刺百会、神门治疗,健康对照组予以指压按摩百会、神门、足三里。记录疗效,ELISA法检测血浆Orexin、CRH、ACTH的含量。结果①治疗前针灸组和对照组比较,针灸组Orexin(112.02±8.95)pg/ml升高(P<0.05),CRH(0.88±0.13)ng/L、ACTH(15.32±4.56)ng/L均显著升高(P<0.01)。②与本组治疗前比较,针灸组治疗后血浆Orexin含量(62.01±6.22)pg/ml降低(P<0.05),CRH(0.41±0.09)ng/L、ACTH(8.01±4.02)ng/L均显著降低(P<0.01)。结论壮医药线点灸联合针刺治疗失眠的起效环节在于抑制Orexin、CRH、ACTH合成和分泌,恢复下丘脑-垂体-肾上腺轴(HPA轴)的正常功能。     

Altered Brain Regional Homogeneity Following Contralateral Acupuncture at Quchi (LI 11) and Zusanli (ST 36) in Ischemic Stroke Patients with Left Hemiplegia: An fMRI Study

Objective: To study the effect of contralateral acupuncture (CAT) at acupoints of Quchi (LI 11) and Zusanli (ST 36) on the unaffected limbs of ischemic stroke patients with left hemiplegia based on regional homogeneity (ReHo) indices. Methods: Ten ischemic stroke patients with left hemiplegia received CAT on right side at LI 11 and ST 36. Functional magnetic resonance imaging (fMRI) was performed before and after acupuncture. A ReHo analytical method was used to compare brain responses of patients before and after CAT operated by REST software. Results: The stimulation at both LI 11 and ST 36 on the unaffected limbs produced significantly different neural activities. CAT elicited increased ReHo values at the right precentral gyrus and superior frontal gyrus, decreased ReHo value at right superior parietal lobule, left fusiform gyrus and left supplementary motor area. Conclusions: Acupuncture at one side could stimulate bilateral regions. CAT could evoke the gyrus which was possibly related to motor recovery from stroke. A promising indicator of neurobiological deficiencies could be represented by ReHo values in post-stroke patients.     

体针、电针配合足底穴位注射治疗顽固性失眠856例临床观察

目的观察体针、电针配合足底穴位注射治疗顽固性失眠的疗效。方法对856例顽固性失眠患者采用体针、电针配合足底穴位注射治疗。体针主要取神门、太冲、足三里、阴陵泉、三阴交、太渊、少府、太溪等穴。电针主要取安眠1、安眠2、安眠3、翳风穴。穴位注射取足跟中点部位敏感点。结果 856例中治愈722例(84.3%),显效123例(14.4%),好转58例(6.8%),总有效率为100.0%。结论体针、电针配合足底穴位注射治疗顽固性失眠具有较好疗效。     

针刺足三里穴对吗啡依赖大鼠条件性位置偏爱的影响

目的:探讨针刺对吗啡依赖性的影响。方法:复制吗啡依赖大鼠模型,观察针刺足三里穴对吗啡依赖大鼠戒断症状及吗啡条件性位置偏爱的影响。结果:针刺组比模型组戒断症状积分明显减少(P<0.01),治疗后针刺组比模型组大鼠在白箱内停留时间减少,差异有显著性。结论:针刺具有抑制吗啡依赖性,对复吸行为具有潜在的干预作用。     

针刺对胃经氧分压和微循环血流灌注量的影响

目的观察常态及针刺时胃经线下深部组织氧分压与微循环血流灌注量的变化,探讨胃经的经与非经、经上穴与非穴相关组织的能量代谢特点。方法沿胃经循行线上取4个穴位(伏兔、阴市、足三里、上巨虚)及2个经上非穴位(伏兔-阴市中点、足三里-上巨虚中点),在6个测试点内外1.5～2 cm处各取1个非经对照点,分为胃经组(胃经穴位组、胃经非穴位组)、内对照点组、外对照点组,连续同步记录氧分压与微循环血流灌注量60 min,观察其在电针前、电针解溪穴时、电针后的变化。结果在正常情况下,沿胃经循行线上测试点深部组织氧分压与微循环血流灌注量显著高于内外两侧非经对照部位,胃经线上测试点氧分压电针解溪穴时及电针后有非常显著降低(P<0.01),胃经穴位组氧分压降低幅度非常显著大于胃经非穴位组(P<0.01);微循环血流灌注量无显著差异;两侧非经对照点无显著变化。结论经络通道上存在着某些特殊的腧穴,在这些腧穴上有着更高的氧气供应,并在经络调控过程中起着重要的作用。     

Effect of acupuncture at three different acupoints on electrical activity of gastric distention-affected neurons in rat medial vestibular nucleus

OBJECTIVE

To observe the effect of gastric distention (GD) and acupuncture at three different acupoints on the spontaneous discharge of neurons in the medial vestibular nucleus (MVN), and to clarify the specific function of the MVN in the central integration mechanism underlying acupuncture regulation.