几种孕妇禁忌中药对早孕绒毛的组织化学及超微结构的影响

本文从形态学及组织化学角度,观察了孕妇禁忌中药商陆、附子及蒲黄对器官培养的早孕绒毛的影响。结果表明:商陆对绒毛的损伤最为严重,光镜观察可见绒毛萎缩变小,滋养层与间质分离,细胞核固缩、溶解、碎裂;胞质减少且嗜酸性增强;电镜下可见,核染色质及核仁溶解,核膜皱缩不规则;各种细胞器损伤较为严重,形态结构难以分辨;胞浆中可见大量的自噬泡和髓样体;组织化学显示,ACP反应增强;ALP反应减弱,3β-HSD、SDH及TPPase反应极弱,甚至阴性。附子对绒毛也有一定程度的损伤,但较商陆为轻。蒲黄对绒毛未见明显损害。     

人早孕绒毛滋养层细胞的分离纯化及马鞭草抗早孕机理的初步研究

目的：探讨马鞭草抗早孕的细胞学作用机理。方法;正常妊娠６－８周早期胎盘Ｐｅｒｃｏｌｌ梯度离心分离出滋养层细胞进行体外培养,观察马鞭草对培养的滋养层细胞形态及绒毛膜促性腺激素分泌功能的影响。结果：２５,５０ｍｇ／ｍｌ马鞭草乙醇提取液对滋养层细胞生长及绒毛膜促性腺激素分泌有明显的抑制作用。结论;一定浓度的马鞭草可直接杀伤滋养层细胞,抑制绒毛膜促性腺激素的分泌而中止早孕。     

抗早孕因子单克隆抗体的制备与鉴定

目的建立分泌抗EPF(Early pregnancy factor，早孕因子)单克隆抗体的杂交瘤细胞株，纯化单抗并鉴定。方法用本实验室已纯化的早孕和肿瘤源性：EPF作为抗原刺激Balb／c小鼠，用免疫后的小鼠脾细胞与同系小鼠骨髓瘤细胞(NS-1)融合，经4次克隆化，获得可稳定分泌抗EPF单克隆抗体的细胞株，注入Balb／c小鼠腹腔制备腹水型单抗，Proteirr-A亲和层析纯化，SDS电泳和Western-blot等方法分析纯化结果。结果融合后获得一株稳定分泌抗EPF抗体的细胞株(C3D11)，克隆化后，获得稳定分泌抗EPF单克隆抗体的细胞株，将增殖后的细胞注射Balb／c小鼠腹腔获得腹水型单抗，以亲和层析法纯化，SDS-PAGE分析显示纯化后去掉了大部分杂蛋白，免疫印迹分析抗体纯度较高，与抗原匹配性良好。结论本研究制备的EPF单克隆抗体为特异性抗EPF抗体。     

Ru486抗早孕剂量与疗效的比较

采用Ru486150mg与Ru486125mg．分别与米索前列醇片200μg×3配伍口服．抗早孕的疗效进行分析比较。结果表明：两种不同的用药方法与剂量，其副反应、完全流产率与孕囊排出的时间相比较无明显差异；但两组药流后阴道出血量有显著性差异。     

中药抗突变实验研究

本文从1984年起分别以C57纯种小鼠的骨髓细胞和人外周血淋巴细胞为实验材料，用公认的诱变剂环磷酰胺(CPP)诱发的姐妹染色单体互换(SCE)，微核(MN)升高作指标，对大量中草药进行实验，已筛选出部分补气药或相关物以及各种人参的有效成分或单体具有抗突变作用。     

无锡地区米非司酮配伍米索前列醇抗早孕失败因素分析

用米非司酮配伍米索前列醇对２１６０例早孕妇女进行终止妊娠，其中失败者共２１３例，经分析表明，其失败的主要因素为：（１）孕妇年龄大于３５岁者，（２）人工流产超过３次以上者，（３）有剖宫产史者，（４）孕妇正处于哺乳期者，（５）妊娠反应较重者。其中人工流产超过３次者及有剖宫产史者，其失败表现多为不全流产或胚胎死亡后不能排出，其余则多表现为继续妊娠     

542例O型血孕妇血清中IgG抗A(B)抗体效价结果分析

目的了解O型血孕妇血清中IgG抗A(B)抗体效价阳性率。方法采用间接抗人球法检测IgG抗A(B)抗体效价。结果检测效价在1∶64以上者达16.7%,且O-A组O-B组阳性率无显著性差异(P>0.05,χ2=1.72)。结论检测孕妇血清中IgG类抗体效价,≥1∶64者及时治疗,可预防和减低新生儿溶血病的发生。     

抗早孕因子单克隆抗体的制备与鉴定

目的建立分泌抗EPF(Early pregnancy factor,早孕因子)单克隆抗体的杂交瘤细胞株,纯化单抗并鉴定.方法用本实验室已纯化的早孕和肿瘤源性EPF作为抗原刺激Balb/c小鼠,用免疫后的小鼠脾细胞与同系小鼠骨髓瘤细胞(NS-1)融合,经4次克隆化,获得可稳定分泌抗EPF单克隆抗体的细胞株,注入Balb/c小鼠腹腔制备腹水型单抗,Protein-A亲和层析纯化,SDS电泳和Western-blot等方法分析纯化结果.结果融合后获得一株稳定分泌抗EPF抗体的细胞株(C3D11),克隆化后,获得稳定分泌抗EPF单克隆抗体的细胞株,将增殖后的细胞注射Balb/c小鼠腹腔获得腹水型单抗,以亲和层析法纯化,SDS-PAGE分析显示纯化后去掉了大部分杂蛋白,免疫印迹分析抗体纯度较高,与抗原匹配性良好.结论本研究制备的EPF单克隆抗体为特异性抗EPF抗体.     

孕妇血清提取物—早孕因子样物质的花结抑制活性测定

以花结抑制试验检测50名妊娠3～8周妇女静脉血混合血清及其经DEAE纤维素离子交换层折和经Sephadex-G-100凝胶过滤后,所得各阶段提取物的花抑滴度,并以HCG胶体金试剂盒测定HCG。对照组为50名处女混合血清,结果证明:混合孕血清经DE-52柱层析后未吸附蛋白部分(DE-I)及Sephadex-G100凝胶过滤后洗脱峰DE-I-GⅡ及DE-I-GⅢ均具花抑活性,DE-I-GⅡ为HCG( )而DE-I-GⅢ为HCG(-)°提示DE-I-GⅡ峰为EPF与HCG混合物,而DE-I-GⅢ峰为不含HCG而具花抑活性的EPF粗提物。     

宫腔钳夹术用于抗早孕的疗效探讨

目的 观察宫腔钳夹术用于药流后孕囊未能排出的妊娠妇女的临床疗效。方法 经常规消毒、窥器暴露宫颈,以18号小头有齿卵圆钳,钳夹出宫腔内容物。结果 360例药流后孕囊未排出的患者经宫腔钳夹术处理,均在当天完全排出孕囊。结论 对药流后孕囊未能排出的妊娠妇女采用宫腔钳夹术处理,可减少药流后的出血时间与出血量,提高完全流产率,确保药流的安全性。     

护理干预对于妊娠糖尿病孕妇妊娠结局的意义

目的：对妊娠糖尿病孕妇采取护理干预的效果探讨。方法对2012年1月~2013年1月80例妊娠糖尿病(GDM)孕妇临床资料进行回顾性分析。结果治疗组的孕产妇及新生儿并发症发生率明显低于对照组,差异具有统计学意义(P<0.05)。结论早期筛查发现,并给予饮食控制、血糖监测、运动疗法、胰岛素治疗、健康教育等正确的临床干预,是改善妊娠结局的关键。     

高三酰甘油血症对BGGI孕妇妊娠结局和并发症的影响

目的评价高三酰甘油(TG)血症对交界性糖耐量受损(BGGI)孕妇妊娠结局和并发症,以及对孕妇产后恢复和新生儿生长的影响。方法216例合并高TG血症的BGGI孕妇作为高TG组,184例TG正常的孕妇作为对照组。比较两组孕妇妊娠结局和新生儿结局,比较产后6周时的FPG、2hPG、HbA1c、TC、低LDL和HDL水平,比较产后6周时的孕妇体重增加、糖尿病发生率和新生儿体重。结果与对照组比较,高TG组的孕妇体重增加和新生儿体重明显偏高,APGAR评分明显偏低,产后出血、剖宫产、GDM和巨大儿发生率上升,差异均具有统计学意义(P<0.05)。产后6周时高TG组的FPG、2hPG、HbA1c、TC和LDL明显高于对照组,差异具有统计学意义(P<0.05)。产后6周时高TG组糖脂代谢异常率、糖尿病发生率、孕妇及新生儿体重均显著高于对照组,差异具有统计学意义(P<0.05)。Pearson相关分析显示产后6周时孕妇体重增加、糖尿病和新生儿体重与FPG、2hPG、HbA1c、TC和LDL呈正相关关系(P<0.05)。结论合并高TG血症的BGGI孕妇不良母婴结局的发生率相对偏高,并且产后糖、脂恢复较慢;糖、脂水平与产后孕妇恢复和新生儿生长发育具有一定的相关性。     

妊娠合并亚甲减孕妇分娩方式对新生儿甲状腺素的影响

目的探讨妊娠合并亚临床甲状腺功能减退孕妇的分娩方式对新生儿甲状腺素的影响。方法随机选择妊娠合并亚临床甲状腺功能减退孕妇活产的足月新生儿107例,包括平产76例,剖宫产31例。测定其脐带血促甲状腺素(thyrotropin,TSH)、总三碘甲腺原氨酸(total triiodothyronine,TT3)和总甲状腺素(total thyroxine,TT4)水平并统计分析。结果妊娠合并亚临床甲状腺功能减退孕妇平产和剖宫产的新生儿脐带血TSH水平的差异有统计学意义(P<0.05),TT3和TT4水平的差异均无统计学意义(P>0.05)。结论剖宫产娩出胎儿未受宫缩及产道挤压的影响,其脐带血TSH水平较平产儿低。     

Validation of a Strict Obesity Definition Proposed for Asians to Predict Adverse Pregnancy Outcomes in Korean Pregnant Women

BackgroundPeople are generally considered overweight and obese if their body mass index (BMI) is above 25 kg/m² and 30.0 kg/m², respectively. The World Health Organization proposed stricter criteria for Asians (≥ 23 kg/m²: overweight, ≥ 25 kg/m²: obese). We aimed to verify whether this criteria could predict adverse pregnancy outcomes in Korean women.MethodsWe included 7,547 Korean women from 12 institutions enrolled between June 2016 and October 2018. Women with no pre-pregnancy BMI data, not Korean, or lost to follow-up were excluded, leaving 6,331. The subjects were categorized into underweight, normal, overweight, class I obesity, and class II/III obesity based on a pre-pregnancy BMI of < 18.5, 18.5–22.9, 23.0–24.9, 25.0–29.9, and ≥ 30.0 kg/m², respectively.ResultsOverall, 13.4%, 63.0%, 11.8%, 9.1%, and 2.6% of women were underweight, normal, and overweight and had class I obesity and class II/III obesity, respectively. In the multivariable analysis adjusted for maternal age, a higher BMI significantly increased the risk of preeclampsia, gestational diabetes, preterm delivery caused by maternal-fetal indications, cesarean section, large for gestational age, and neonatal intensive care unit admission.ConclusionAdverse pregnancy outcomes started to increase in those with a pre-pregnancy BMI ≥ 23.0 kg/m² after adjusting for maternal age. The modified obesity criteria could help predict adverse pregnancy outcomes in Koreans.     

优质护理干预对高危妊娠产妇心理状态及妊娠结局的影响

目的 探究优质护理干预对高危妊娠产妇心理状态及妊娠结局的影响。方法 选取本院2014年1月~2017年1月收治的76例高危妊娠产妇,采用随机数表法将其分为对照组和观察组,每组38例。对照组进行常规护理,观察组进行优质护理干预,对两组产妇的心理状态、妊娠结局及护理满意度进行对比。结果 观察组在进行优质护理干预后,产妇焦虑比例为21.05%,低于对照组的44.74%,差异具有统计学意义（P＜0.05）;观察组妊娠结局优于对照组,差异具有统计学意义（P＜0.05）;观察组护理满意度为81.58%,高于对照组的52.63%,差异具有统计学意义（P＜0.05）。结论 优质护理干预有利于改善产妇的心理状态,减少不良妊娠结局,增强产妇和胎儿的安全,且可较好提升产妇的护理满意度,有助于提升护理质量及医院的发展。     

人绒毛膜促性腺激素对早孕妇女甲状腺机能的影响

本文应用放射免疫（ＲＩＡ）和免疫放射（ＩＲＭＡ）方法对２０例早孕妇女的人绒毛膜促性腺激素（ｈＣＧ）水平进行研究，以求阐明是否ｈＣＧ具有垂体促甲状腺激素（ＴＳＨ）样作用的可能性。结果显示，甲孕妇女四碘甲状腺原氨酸（Ｔ４）和游离甲状腺素（ＦＴ４）水平升高，ｈＣＧ浓度与Ｔ４、ＦＴ４亦成正相关关系，然其三碘甲状腺原氨酸（Ｔ３）、游离三碘甲状腺原氨酸（ＦＴ３）水平却与正常对照组无明显差异。ｈＣＧ水平升高与Ｔ     

Prospective Cohort Study of the Safety of an Influenza A(H1N1) Vaccine in Pregnant Chinese Women

To monitor and evaluate the safety of the influenza A(H1N1) vaccine in pregnant women and its influence on the fetus and neonate, we performed a prospective study in which 122 pregnant Chinese women who received the influenza A(H1N1) vaccine and 104 pregnant women who did not receive any vaccine (serving as controls) were observed. The results indicated that the seroconversion rate in the vaccinated group was 90.4% (95% confidence interval [CI], 82.6% to 95.5%). The rate of adverse events following immunization in the pregnant women who received the influenza A(H1N1) vaccine was 3.3%. The spontaneous abortion rates in the vaccinated group and the unvaccinated group were 0.8% and 1.9%, respectively (exact probability test, P = 0.470), the prolonged-pregnancy rates were 8.2% and 4.8%, respectively (χ² = 1.041, P = 0.308), the low-birth-weight rates were 1.6% and 0.95%, respectively (exact probability test, P = 1.000), and the spontaneous-labor rates were 70.5% and 75%, respectively (χ² = 0.573, P = 0.449). All newborns who have an Apgar score of ≥7 are considered healthy; Apgar scores of ≥9 were observed in 38.5% and 57.7% of newborns in the vaccinated group and the unvaccinated group, respectively (χ² = 8.274, P = 0.004). From these results, we conclude that the influenza A(H1N1) vaccine is safe for pregnant women and has no observed adverse effects on fetal growth. (This study has been registered at ClinicalTrials.gov under registration no. {"type":"clinical-trial","attrs":{"text":"NCT01842997","term_id":"NCT01842997"}}NCT01842997.)     

Mechanism of action of benzene on hematopoiesis (investigation of hematopoietic stem cells)

The effect of benzene on colony-forming activity of the bone marrow and spleen was studied in four groups of experiments. In benzene hypoplasia of hematopoiesis, fewer colony-forming units were found in the hematopoietic organs although there was no change in the distribution of their cell types. Incubation of normal bone marrow cells with benzene did not reduce the efficiency of colony formation. Injection of benzene into lethally irradiated mice after transplantation of a suspension of normal bone marrow cells into the animals caused a sharp decrease in colony production, with inhibition chiefly of the formation of granulocytic colonies. These observations suggest that during benzene poisoning the properties of the microenvironment of the hematopoietic stem cell are modified.Presented by Academician of the Academy of Medical Sciences of the USSR A. A. Minkh.Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 82, No. 7, pp. 797–799, July, 1976.     

妊娠期妇女血清甘胆酸与血脂水平相关性研究

目的:探讨妊娠期妇女肝内胆汁淤积症(ICP)的发病率及甘胆酸(CG)与血脂、碱性磷酸酶(ALP)水平的相关性。方法:采用放射免疫分析检测甘胆酸,用酶法测定血脂,速率法测定ALP。结果:2040例妊娠期妇女中有18.5%CG值升高,其中有2.8%可确诊为ICP。ICP患者与正常妊娠组相比,TG、CHOL、LDL、ALP均明显升高,而HDL则略降低。结论:ICP为妊娠期常见并发症,应将CG作为常规产前检查项目。ICP患者结合分析其血脂、ALP水平,有助于病情判断。