掌背动脉逆行岛状皮瓣的临床应用

目的探讨掌背动脉逆行岛状皮瓣修复手指皮肤缺损的方法。方法基于掌背动脉与指掌侧动脉存在交通支这一解剖基础,本组设计以此交通支为蒂,带第1或第2或第3或第4掌背动脉的手背逆行岛状皮瓣,修复食、中、环、小指掌、背侧皮肤缺损28例。结果28例术后皮瓣全部成活,皮瓣色泽、厚度及质地与受区皮肤接近,手部外形及功能恢复满意,皮瓣两点辨别觉6mm-9mm。结论掌背动脉逆行岛状皮瓣修复手指皮肤缺损操作简单,有广泛的临床应用价值,是修复手指掌背侧近节或近2节指皮肤缺损较理想的方法。     

掌骨背动脉岛状皮瓣修复指掌面皮肤缺损21例

目的:探讨手指掌面皮肤缺损的修复方法。方法:在手背设计掌骨背动脉岛状皮瓣,逆行翻转修复2、3、4、5指中节以近掌面不同程度的皮肤缺损共21例(30指),皮瓣缺陷范围0.5cm×1.0cm￣2.5cm×3.0cm。结果:患者植皮区及供皮区均一期愈合,随访3个月￣2年,所有患者手指功能正常,无疤痕挛缩。结论:掌骨背动脉岛状皮瓣是指掌面皮肤缺损修复较理想的选择。     

逆行掌背动脉岛状皮瓣修复手指皮肤缺损41例

目的探讨逆行掌背动脉岛状皮瓣修复手指皮肤缺损的治疗效果。方法回顾性分析我院2005年3月至2011年11月利用逆行掌背动脉岛状皮瓣修复手指近中节皮肤软组织缺损41例46指,其中近节33指,中节13指。结果本组41例46指,术后皮瓣全部成活,皮瓣颜色、厚度与受区皮肤一致,手指外形及功能恢复好。结论逆行掌背动脉岛状皮瓣是修复手指近中节小面积皮肤缺损较好的方法之一。     

指背动脉蒂逆行岛状皮瓣修复指端皮肤缺损的临床研究

目的 介绍一种修复手指末端皮肤缺损的新型逆行岛状皮辩.方法 应用指背动脉为蒂,设计成逆行岛状皮瓣,从2002年6月～2006年10月,临床应用掌背指背动脉蒂逆行岛状皮瓣修复手指末端皮肤缺损30例,皮瓣面积1.2 cm×1.4 cm～2.4cm×3.2 cm.结果 临床应用30例,皮瓣全部成活,效果满意.结论 以指背动脉为蒂设计成逆行岛状皮瓣,该皮瓣设计合理、血供可靠、操作简便,适用于手指末端软组织缺损的修复.     

第二掌背动脉逆行岛状皮瓣修复示指皮肤缺损26例的报告

目的:第二掌背动脉逆行岛状皮瓣是修复示指近、中节皮肤缺损适用的方法之一.方法:我科从2007年6月～2011年10月应用第二掌骨背逆行筋膜蒂皮瓣修复示指皮肤26例,以第二掌背动脉为轴线,以该动脉远端及筋膜为蒂.结果:术后26例皮瓣全部成活,经2～15个月随访,手指功能及外观均良好.结论:第二掌背动脉逆行岛状皮瓣设计合理,血供可靠,适于修复示指近节及中节掌背侧皮肤软组织缺损.     

第二掌背动脉双轴点逆行岛状筋膜皮瓣的临床应用

目的研究临床应用第二掌背动脉双轴点逆行岛状筋膜皮瓣的方法和疗效。方法利用第二掌背动脉远端穿支和进入掌背筋膜皮瓣近端的第二掌背动脉近端穿支附近的小血管分支，形成两个旋转点的第二掌背动脉双轴点逆行筋膜皮瓣，修复拇指、环指、小指背侧皮肤缺损各1例，示指、中指背侧皮肤缺损各2例，中指掌侧皮肤缺损2例。急诊手术7例，急诊延迟手术2例。结果9例皮瓣全部成活，修复的手指外形满意，指关节伸屈同健侧。结论第二掌背动脉双轴点逆行岛状筋膜皮瓣旋转范围比第二掌背动脉岛状皮瓣大，可修复手较大范围内的创面。     

岛状皮瓣在四肢损伤修复中的应用

我们自1995年1月～2000年1月应用岛状皮瓣修复手部创伤12例,修复下肢损伤3例,效果满意,现报告如下. 临床资料 本组15例,男14例,女1例,年龄18～56岁,平均37岁.急诊手术12例,择期手术3例,均为外伤造成软组织缺损,无法用皮片移植或轴型皮瓣转移来修复,而采用岛状皮瓣修复.皮瓣种类:前臂桡动脉逆行岛状皮瓣,修复拇指及第一掌骨开放性骨折处皮肤及软组织缺损2例,修复手掌部及拇指间隙软组织缺损1例,手背2例;指侧方指固有动脉逆行岛状皮瓣修复手指背侧及末节软组织缺损4例;示指近节背侧第一掌背动脉岛状皮瓣修复拇指皮肤及软组织缺损3例;足背动脉岛状皮瓣修复内、外踝处皮肤及软组织缺损各1例;小腿内侧动脉岛状皮瓣修复胫前溃疡1例.皮瓣面积最小为1.5cm×1.5cm,最大为13cm×7cm,皮瓣供区均采用中厚皮片移植.15例皮瓣完全成活.随访1年2个月,功能及外形恢复满意.     

手指皮肤缺损修复效果观察

目的:观察手指皮肤缺损的修复效果。方法:对32指手部不同部位的皮肤缺损采用了不同的皮瓣修复。其中皮神经岛状皮瓣逆行转位重建拇指感觉功能16指,中指尺侧岛状皮瓣加示指背侧岛状皮瓣转位修复拇指套脱伤2指,中环指岛状皮瓣转位修复拇指撕脱伤4指,以手指一侧指背动脉为轴转位修复手指掌侧近中节皮扶缺损10指。结果:除有一例拇指皮肤边缘部分坏死外,其余全部成活。经随访6个月-7年,拇指及指掌侧皮瓣的质地、外观、功能、长度和感觉良好。结论:皮神经岛状皮瓣逆行转位,中指尺侧岛状皮瓣加示指背侧岛状皮瓣转位,中环指岛状皮瓣及指背皮瓣转位修复拇指损伤及手指掌侧近中节皮肤缺损,方法简单、可靠,手指的外观、感觉、功能满意。     

指动脉皮瓣及掌背动脉皮瓣修复手指皮肤缺损

目的对应用指动脉皮瓣及掌背动脉皮瓣修复手指皮肤缺损临床效果进行观察。方法选择指动脉及掌背动脉岛状皮瓣修复手指皮肤缺损14例,其中2例2指同时行两种逆行岛状皮瓣,其余均行1种岛状皮瓣。结果本组14例16个皮瓣中,有2例掌背动脉皮瓣远端部分坏死,经换药后伤口愈合,余皮瓣全部成活良好,术后随访3个月-3年,皮瓣血运、弹性、质地及外形良好。结论对手指不同部位组织缺损,选择指动脉及掌背动脉岛状皮瓣进行修复,能获得较为理想的治疗效果。     

掌背动脉逆行岛状皮瓣修复手指皮肤缺损

目的:分析应用掌背动脉逆行岛状皮瓣修复手指皮肤缺损的疗效。方法:应用掌背逆行岛状皮瓣18例用以修复手指皮肤缺损,观察皮瓣成活率、质地、色泽和感觉。结果:术后皮瓣肿胀3例,静脉危象2例,18例皮瓣全部成活,术后随访3~12个月,平均8个月,皮瓣外观、质地、弹性良好。并有部分感觉恢复,部分感觉良好。结论:掌背动脉逆行岛状皮瓣不损伤主要血管、神经,操作简单,是修复手指创面的理想方法。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.