生脉注射液联合黄芪注射液治疗缓慢性心律失常106例疗效分析

目的观察生脉注射液联用黄芪注射液治疗缓慢性心律失常疗效及安全性.方法选取我院106例缓慢性心律失常患者,分为A(35例)、B(36例)、C(35例)三组.A组给予生脉注射液40ml+黄芪注射液40ml/日稀释后静脉滴注,14天为一疗程,B组单独给予生脉注射液40ml /日,C组单独给予黄芪注射液40ml/日,14天为一疗程,观察治疗后患者临床症状、心电图的变化.结果治疗组临床疗效总有效率97.1%,24小时动态心电图有显著差异,与对照组比较差异有高度统计学意义(P<0.05).结论生脉注射液联用黄芪注射液治疗缓慢性心律失常有效,在提高心率上优于单独使用,且副作用少,临床应用安全.     

生脉注射液联用氨茶碱注射液治疗缓慢性心律失常60例疗效观察

目的 观察生脉注射液联用氨茶碱注射液治疗缓慢性心律失常疗效。方法对60例缓慢性心律失常患者给与生脉注射液30ml／日＋氨茶碱注射液0．25～05ml／日稀释后静脉滴注14天为一疗程,观察治疗后患者临床症状、心电图的变化。结果治疗前后临床疗效总有效率85％,24小时动态心电图有显著差异。结论生脉注射液联用氨茶碱注射液治疗缓慢性心律失常有效。     

黄芪、生脉注射液治疗心律失常的临床疗效观察

目的观察黄芪和生脉注射液对轻型心律失常的临床疗效。方法将100例心律失常病人随机分为治疗组和对照组,全部病例均经静息心电图或24小时动态心电图证实有不同类型和程度的心律失常,在综和治疗的基础上,治疗组加用黄芪和生脉注射液,对照组加用倍他乐克、心律平、利多卡因等常规制剂。结果与对照组（总有效率72％）相比,治疗组（总有效率96％）对患者临床症状的改善及心律失常的疗效均优于对照组（P〈0.05）。结论黄芪和生脉注射液对轻型的心律失常有较明显的治疗效果。     

参仙升脉口服液治疗西宁地区缓慢性心律失常疗效观察

目的：观察参仙升脉口服液治疗缓慢性心律失常的临床疗效。方法：160例缓慢性心律失常患者随机分为两组。治疗组80例：口服参仙升脉口服液,每次20mL,每日2次;对照组80例：口服心宝丸,每次4粒,每日2次,两组均4周为1个疗程。服药前及1个疗程后均行24小时动态心电图检查,并观察疗效及不良反应。结果：治疗组临床症状改善优于对照组,24小时心电图改善情况优于对照组（P〈0.05）,两组均有较少不良反应。结论：参仙升脉口服液是治疗高海拔地区缓慢性心律失常安全而有效的药物。     

炙甘草汤加减治疗心律失常48例临床疗效观察

目的探讨炙甘草汤治疗缓慢性心律失常的临床疗效。方法将48例缓慢性心律失常患者随机分为治疗组和对照组。对照组给予常规西药治疗,治疗组仅给予炙甘草汤治疗,将两组治疗前后临床症状改善情况、心电图、动态心电图变化情况进行对照观察。结果治疗组临床症状改善有效率为83.3%,心电图改善有效率为75.0%;对照组临床症状改善有效率为50.0%,心电图改善有效率为54.2%,两组间有效率比较差异有统计学意义（P〈0.05）。结论炙甘草汤加减能有效提高缓慢性心律失常患者平均心率水平,减轻心肌缺血,改善临床症状。     

中西医结合治疗病态窦房结综合征疗效观察

目的观察生脉注射液与西医联合治疗病态窦房结综合征临床疗效。方法将50例病态窦房结综合征病人随机分为治疗组和对照组,全部病例均经心电图、24小时动态心电图,部分患者经阿托品试验和食管调搏检查证实,对照组常规基础治疗,治疗组在基础治疗上加用生脉注射液。结果与对照组（总有效率75%）相比,治疗组（总有效率92.3%）对患者临床症状的改善及检测的相关指标疗效均优于对照组（P〈0.05）。结论生脉注射液结合西医常规治疗对病态窦房结综合征有较明显的治疗效果。     

参附注射液治疗缓慢性心律失常60例疗效观察

目的 探讨参附注射液对缓慢型心律失常的疗效.方法 将120例患者随机分为对照组,治疗组两组,在常规治疗缓慢性心律失常的基础上,对照组60例参附注射液80ml静脉滴入,治疗组60例氨茶碱注射液0.125g静脉滴入.两组在治疗前及治疗后第3个月和第6个月对所入选患者进行详细体检,并分别行动态心电图检查.治疗期间严密观察不良反应并记录.结果 与治疗前相比,两组心动过缓均有明显改善,两组在治疗后,心率较用药前增加20%以上或动态心电图检查24小时总心搏达9万次以上,而治疗组较对照组更为明显,而不良反应的发生两组无明显差异.结论 参附注射液对缓慢性心律失常的疗效明显优于对照组,未发现明显毒副作用或致心律失常作用.长期应用能减少猝死的发生率,降低死亡率,改善预后.     

参松养心胶囊治疗缓慢性心律失常临床观察

目的：探索应用参松养心胶囊治疗缓慢性心律失常的临床疗效。方法：将根据24h动态心电图确诊的78例缓慢性心律失常（病态窦房结综合征、房室传导阻滞、慢一快综合征、窦性心动过缓等）患者随机分为两组,参松养心胶囊组（治疗组）和常规治疗组（对照组）。治疗组在常规治疗基础上加用参松养心胶囊,疗程6周。观察两组的总有效率、治疗前后患者24h平均心率变化的绝对值和变化率、症状改善情况。结果：两组治疗缓慢性心律失常总有效率分别为治疗组62．5％和对照组21．1％（P〈0．01）。结论：参松养心胶囊治疗缓慢性心律失常的临床疗效确切,副作用     

参仙升脉口服液治疗老年人缓慢性心律失常的疗效观察

目的:观察参仙升脉口服液治疗老年人缓慢性心律失常的疗效。方法:选择老年人缓慢性心律失常患者96例,随机分为参仙升脉组、心宝丸组、氨茶碱组。参仙升脉组:口服参仙升脉口服液,每次20ml,2次/日;心宝丸组:口服心宝丸,每次4丸(240mg),2次/日;氨茶碱组:口服氨茶碱,每次0.2g,3次/日。4周为1个疗程,观察用药前后临床症状、心电图、24小时动态心电图。结果:(1)每组治疗前后比较,24小时总心率、最快心率、最慢心率、平均心率、早搏数均明显增加,P<0.05;治疗后参仙升脉组的24小时总心率、最快心率、最慢心率、平均心率、早搏数与心宝丸组、氨茶碱组比较,P<0.05,均有显著性差异。(2)治疗4周后,三组心电学疗效方面,参仙升脉组显效21例、有效6例,总有效率84.4%;心宝丸组显效12例、有效8例,总有效率62.5%;氨茶碱组显效14例、有效9例,总有效率71.9%。参仙升脉组与心宝丸组、氨茶碱组比较P<0.05。(3)治疗4周后,三组临床症状疗效方面,参仙升脉组显效18例、有效10例,总有效率87.5%;心宝丸组显效16例、有效5例,总有效率65.6%;氨茶碱组显效12例、有效8例,总有效率62.5%。参仙升脉组与心宝丸组、氨茶碱组比较P<0.05。结论:参仙升脉口服液治疗老年人缓慢性心律失常疗效明显优于心宝丸或氨茶碱。     

参松养心胶囊治疗缓慢性心律失常临床观察

目的：观察参松养心胶囊治疗缓慢性心律失常的临床疗效。方法：60例缓慢性心律失常患者随机分为2组。治疗组30例：参松养心胶囊口服,每次4粒,每日3次;对照组30例：心宝丸口服,每次4粒,每日2次,2组均4周为1个疗程。服药前及1个疗程后均行24小时动态心电图检查,并观察疗效及不良反应。结果：治疗组临床症状改善优于对照组,心电图改善情况优于对照组（P〈0.05）,两组均有较少不良反应。结论：参松养心胶囊是治疗缓慢性心律失常安全而有效的药物。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.