Context The intentional use of
Bacillus anthracis transmitted
via the US mail in October-November 2001 resulted in 22 people
developing inhalation or cutaneous anthrax. Glove use with handwashing
prior to and after contact with potential contaminated environmental
surfaces and cutaneous lesions has been recommended. However,
only limited data are available on the susceptibility of
B anthracis to antiseptics.
Objective To evaluate the efficacy of several hand antiseptics
(interventions) and soap and water (control) against
Bacillus atrophaeus, a surrogate of
B anthracis.
Design, Setting, and Participants Challenge study conducted
among healthy adult volunteers, using the Standard Test Method
for Evaluation of the Effectiveness of Health Care Professional
Handwash Formulations (American Society for Testing and Materials
E 1174-94) to determine the efficacy of various hand hygiene
products at wash times of 10, 30, and 60 seconds. Volunteers
were excluded if they had eczema, psoriasis, or other chronic
skin conditions; nonintact skin; or allergies to any study agent.
Study agents were a waterless rub containing 61% ethyl alcohol,
a 2% chlorhexidine gluconate preparation, and an antibacterial
microfiber towel that releases hypochlorite. A nonantimicrobial
soap was used as a control.
Main Outcome Measure Reduction of
B atrophaeus spores
(log
10 CFU/mL) on contaminated hands.
Results Washes of 10, 30, and 60 seconds with either soap
and water or 2% chlorhexidine gluconate eliminated 1.5 to 2.0
log
10 CFUs/mL of
B atrophaeus spores at wash 3. Mean reductions
(95% confidence intervals) with 10-, 30-, and 60-second washes
with soap and water were 2.4 (2.2-2.5), 2.3 (2.2-2.4), and 2.1
(1.9-2.4) log
10 CFUs/mL, respectively; and with 2% chlorhexidine
gluconate, 2.1 (2.0-2.3), 1.8 (1.5-2.0), and 1.7 (1.5-1.9) log
10 CFUs/mL, respectively. Handwashing with chlorine-containing
towels was increasingly effective as the wipe time increased;
reductions at 10, 30, and 60 seconds were 1.3 (1.1-1.5), 1.6
(1.2-2.0), and 2.2 (2.1-2.2) log
10 CFUs/mL, respectively. A
waterless rub containing 61% ethyl alcohol was ineffective in
eliminating
B atrophaeus spores at all times tested (0 [-0.1
to 0.1], -0.2 [-0.3 to -0.1], and 0 [-0.2 to 0.2] log
10 CFUs/mL).
Conclusions In this evaluation of hand hygiene agents,
handwashing with soap and water, 2% chlorhexidine gluconate,
or chlorine-containing towels reduced the amount of
B atrophaeus spore contamination, whereas use of a waterless rub containing
ethyl alcohol was not effective in removing spores.
相似文献