海蛾提取物对小鼠免疫器官重量和抗应激能力的影响

海蛾提取物使氢化可的松或环磷酰胺所致免疫低下小鼠的脾脏重量和胸腺重量增加,使老龄小鼠胸腺重量增加;海蛾提取物显著延长小鼠在常压缺氧或氰化钾所致化学性缺氧条件下的存活时间,对小鼠冰水中游泳存活时间也有显著的延长作用,并可明显提高小鼠耐饥渴能力。     

粗根荨麻提取物对环磷酰胺所致小鼠免疫功能低下保护作用的实验观察

目的 研究粗根荨麻提取物对环磷酰胺所致小鼠免疫功能低下保护作用.方法 通过测定小鼠血清溶血素、小鼠IL-2、IFN-γ、CD3、CD4、CD8的水平,研究粗根荨麻提取物的免疫调节作用.结果 粗根荨麻提取物可提高小鼠血清溶血素、小鼠IL-2、IFN-γ、CD3、CD4、CD8的水平.结论 粗根荨麻提取物具有增强机体免疫功能的作用.     

山药多糖对环磷酰胺致小鼠脾脏损伤的保护作用

目的:探究山药多糖对环磷酰胺所致小鼠脾脏损伤的保护作用。方法:以腹腔注射环磷酰胺的方式制备小鼠免疫低下模型,分别应用中剂量和高剂量山药多糖灌胃。观察山药多糖对小鼠体重、肝脏指数、白细胞计数变化的影响,并观察小鼠脾脏组织病理学改变。结果:与对照组相比,环磷酰胺组小鼠在T9～T15期间体重明显降低,脾脏指数和白细胞计数显著降低(P0.05),并且造成严重的脾组织病理学损害。与环磷酰胺组相比,山药多糖中剂量和高剂量组小鼠脾脏指数和白细胞计数明显改善(P0.05),且肝组织病理学损伤明显减轻。结论:中剂量和高剂量山药多糖对环磷酰胺所致小鼠肝脾损伤具有良好的保护作用。     

毛木耳补血作用的实验研究

目的研究毛木耳对贫血小鼠的补血作用。方法采用乙酰苯肼(APH)与环磷酰胺(CTX)复合溶血法、失血法、环磷酰胺化学损伤法分别复制不同的贫血模型,观察毛木耳的补血作用。结果毛木耳能升高溶血和失血所致贫血模型小鼠外周血红细胞、血红蛋白水平,升高环磷酰胺损伤小鼠的白细胞水平。结论毛木耳具有较好的补血作用。     

天麻提取物对小鼠学习记忆能力的影响

用小鼠跳台法实验观察了天麻提取物对由东莨菪碱、亚硝酸钠、乙醇所致的小鼠记忆损伤病理模型的影响，结果显示天麻提取物对小鼠学习记忆能力具有明显的改善作用。     

再生汤治疗再障的实验研究

实验表明,“再生汤”对环磷酰胺所致骨髓造血机能损伤的小鼠有治疗作用,能使骨髓有核细胞增殖,Hb、RC、WBC、PTC升高,有的趋近于正常。小鼠预先灌服“再生汤”,对环磷酰胺导致的骨髓造血系统抑制、损伤,具有保护作用。     

海蛾提取物对小鼠免免疫器官重量和抗应激能力的影响

海蛾提取物使氢化可的松或环磷酰胺所致免疫低下小鼠的脾脏重量和胸腺重量增加,使老龄小鼠胸腺重量增加;海蛾提取物显著延长小鼠在常压缺氧或氰化钾所至化学性缺氧条件下的存活时间,对小鼠冰水中游泳存活时间也有显著的延长作用,并可明显提高上鼠耐饥渴能力。     

胡桃楸树皮乙醇提取物的抗氧化研究

目的探讨胡桃楸树皮乙醇提取物(AEBJ)对X线照射损伤小鼠的抗氧化活性。方法将60只雄性健康昆明小鼠随机分为假照射组,照射对照组,高、低剂量照射给药组和高、低剂量单纯给药组共六个试验组,测定小鼠肝组织匀浆中H2O2与MDA的含量。结果高、低剂量AEBJ照射给药组均能抑制照射所致的H2O2的含量增高,高剂量AEBJ照射给药组能抑制照射所致的MDA含量增高,低剂量AEBJ照射给药组对照射所致的MDA含量无影响。结论胡桃楸树皮乙醇提取物对X线照射损伤小鼠具有一定的抗氧化活性。     

复方党参提取物对环磷酰胺处理小鼠免疫功能的调节作用

目的:观察复方党参提取物对环磷酰胺所致免疫低下小鼠免疫功能的调节作用。方法:小鼠随机分为对照组、模型组和复方党参提取物低、中、高剂量组(0.25,0.5,1.0 g.kg-1),各剂量组、对照组和模型组均ig给药,持续10 d,并在第7天开始同时ip环磷酰胺制作免疫抑制小鼠模型。MTT法检测免疫细胞的活力,鸡红细胞吞噬检测巨噬细胞的吞噬功能。绵羊红细胞免疫法检测小鼠体液免疫功能。结果:复方党参提取物各组脾淋巴细胞转化率升高、足跖厚度增加;脾细胞溶血空斑数增加、血清溶血素水平及腹腔巨噬细胞吞噬率增加;复方党参提取物中、高剂量组与模型组比较有非常显著性差异(P<0.01)。结论:复方党参提取物对环磷酰胺处理的免疫低下小鼠有免疫保护作用。     

印度人参根提取物对免疫低下小鼠免疫功能的影响

目的：印度人参根提取物(IRRE)对免疫低下小鼠的免疫调节作用。方法：给免疫低下小鼠灌胃印度人参根提取物5，2.5，0.85 g·kg^-1体重，观察其对免疫低下小鼠免疫功能的影响。结果：印度人参根提取物可减缓由环磷酰胺引起的生长缓慢和脏器指数下降，对小鼠迟发型变态反应和抗体生成细胞有增强作用，在刀豆球蛋白A(ConA)刺激的淋巴细胞转化实验中，印度人参根提取物各剂量组显著高于环磷酰胺组(P<0.05)。各剂量组骨髓DNA含量、血清一氧化氮(NO)含量显著高于空白组和环磷酰胺组，但磷酸酶活性变化不大。结论：印度人参根提取物对由环磷酰胺导致的巨噬细胞吞噬功能下降和脾脏损伤有一定恢复能力，能有效保护环磷酰胺所导致的骨髓抑制作用。     

针灸对小鼠骨髓细胞微核的影响

目的 :研究针灸对小鼠骨髓细胞微核的影响。方法 :将实验动物分为正常对照组 ,阳性对照Ⅰ、Ⅱ组 ,针灸预防Ⅰ、Ⅱ、Ⅲ、Ⅳ组 ,针灸治疗Ⅰ、Ⅱ组。针刺小鼠“足三里” ,艾灸“关元”穴 ,检测小鼠骨髓嗜多染红细胞微核率、骨髓有核细胞微核率的变化。结果 :阳性对照组与正常对照组比较 ,小鼠骨髓嗜多染红细胞微核率、骨髓有核细胞微核率明显升高。针灸预防Ⅰ、Ⅱ、Ⅲ、Ⅳ组 ,针灸治疗Ⅰ组与阳性对照Ⅰ组比较 ,针灸治疗Ⅱ组与阳性对照Ⅱ组比较 ,各指标明显降低 (P <0 0 0 1)。结论 :针灸可拮抗由环磷酰胺诱导的小鼠骨髓嗜多染红细胞微核率、骨髓有核细胞微核率的升高     

杜仲总多糖对环磷酰胺致免疫低下小鼠的影响

目的研究杜仲总多糖对环磷酰胺致免疫低下小鼠的影响。方法应用环磷酰胺建立免疫低下小鼠模型,给予不同剂量杜仲总多糖后,测定小鼠体重、胸腺指数、脾指数、腹腔巨噬细胞吞噬率、吞噬指数等指标。结果300g杜仲原药材经提取分离得9.3g多糖干品。应用环磷酰胺后,小鼠体重、胸腺指数、腹腔巨噬细胞吞噬率、吞噬指数均降低。杜仲总多糖能减轻环磷酰胺致小鼠体重的下降,升高免疫低下小鼠胸腺指数,明显增加小鼠腹腔巨噬细胞吞噬率、吞噬指数(P<0.01)。与空白组比较,杜仲总多糖还能提高正常小鼠的脾指数(P<0.05)。结论杜仲总多糖能够较好地增强小鼠免疫功能。     

Protective Effects of Total Flavonoids from Epimedium on the Male Mouse Reproductive System Against Cyclophosphamide‐Induced Oxidative Injury by Up‐Regulating the Expressions of SOD3 and GPX1

Total flavonoids of Epimedium (TFE) is the main active composition of Epimedium that has been used to treat male reproductive problems. The present aim was to investigate the protective effects of TFE on male mice reproductive system against cyclophosphamide (CP)‐induced oxidative injury. The animals were treated with CP to make testicular injury model and the protective effects of TFE were observed. In the CP‐treated group, testicular and epididymal weights, sperm count and motility significantly decreased relative to the control group (P < 0.05 and P < 0.01, respectively). Compared with the CP‐treated group, TFE (200 and 400 mg/kg) treated mice increased testicular weights by 21.6% and 28.4% (P < 0.05), sperm counts by 81.7% and 148.3% (P < 0.01) and sperm motility by 47.2% and 61.3% (P < 0.01). Meanwhile, the CP‐treated group showed enhancement of lipid peroxidation leading to testicular reproductive toxicity. TFE restored these oxidative damages by up‐regulating the expression of antioxidant enzymes, especially SOD3 and GPX1. TUNEL assay and histopathological observations provided supportive evidence for above results, and when the dose of TFE increased, the aforesaid improvement became more and more strong. These results demonstrated that TFE exerted beneficially protective effects on the structural and functional damage of male mice reproductive system and reduced apoptosis in spermatogenic cells by inhibiting CP‐induced oxidative stress. Copyright © 2013 John Wiley & Sons, Ltd.     

Protective effect of a polyherbal formulation (Immu-21) against cyclophosphamide-induced mutagenicity in mice

The object was to evaluate the effects of a polyherbal formulation, Immu-21, against cyclophosphamide (CP)-induced chromosomal aberrations (CA) and micronuclei (MN) in mice. CP alone (40 mg/kg, i.p.) produced classical as well as non-classical chromosomal aberrations in mice, and the incidence of CA was significantly more in the CP treated group when compared with that of the control group. Immu-21, which contains extracts of Ocimum sanctum, Withania somnifera, Emblica officinalis and Tinospora cordifolia, was given at 100 mg/kg, daily, over 7 days, and 30 mg/kg daily over 14 days and inhibited both CP-induced classical and non-classical chromosomal aberrations ( approximately 40%-60% of control). A significant increase in MN was also observed in bone marrow erythrocytes of mice treated with CP, and pretreatment with Immu-21 also significantly reduced these. Cytotoxicity was evaluated by estimating the ratio of polychromatic erythrocytes (PCEs) to normochromatic erythrocytes (NCEs). The present results indicate that chronic treatment with Immu-21 prevented CP-induced genotoxicity in mice.     

真菌植物松茸提取物抑制环磷酰胺致突变作用研究

目的:寻找安全有效的抗突变生物资源,为肿瘤预防和降低环磷酰胺的遗传危害提供科学依据.方法:采用修改的Ames试验和小鼠骨髓细胞微核试验,研究松茸提取物AMH对CP致突变作用的影响.结果:Ames试验结果表明,CP具有碱基对置换致突变作用,AMH能显著性抑制CP的致突变作用(P＜0.01),对测试菌株TA100的诱发回变菌落形成抑制率最高为79.9%;小鼠骨髓细胞微核试验也表明,AMH能显著性抑制CP对遗传物质的损害,微核形成抑制率达82%.AMH对CP致突变作用的抑制作用存在明显的剂量反应关系.结论:松茸提取物对CP致突变作用具有显著的抑制作用,在肿瘤预防和降低CP的毒副作用方面具有应用前景.     

杜仲多糖镇痛作用研究

目的研究杜仲多糖对小鼠的镇痛作用。方法各组小鼠连续灌胃不同剂量的杜仲多糖7 d,末次给药后1 h,以己烯雌酚和缩宫素诱发月经痛、辐射热致痛、热板致痛法、醋酸扭体法为疼痛模型,生理盐水为阴性对照药,阿司匹林为阳性对照药,考察杜仲多糖的镇痛作用。结果杜仲多糖能显著延长辐射热致痛模型中小鼠的痛阈(P<0.01),而对热板致痛、醋酸致痛、痛经模型有一定的抑制作用。结论杜仲多糖有一定的镇痛作用。     

Anticlastogenic effects of Hibiscus sabdariffa fruits against sodium arsenite-induced micronuclei formation in erythrocytes in mouse bone marrow

An in vivo micronucleus assay using albino mice was used to examine the anticlastogenic effects of a crude aqueous extract of Hibiscus sabdariffa fruits in bone marrow cells of mice. Various doses of freshly prepared crude extract of Hibiscus sabdariffa (50, 100 and 150 mg/kg b.w.) were given by gavaging to male laboratory bred Swiss albino mice for 7 days as a dietary supplement followed by a single dose of sodium arsenite (2.5 mg/kg b.w.) After 24 h, the animals were killed and bone marrow smears were prepared and stained in Giemsa. The results show that sodium arsenite effectively induced micronuclei in polychromatic erythrocytes (PCEs). Administration of a crude extract of Hibiscus sabdariffa led to a significant reduction of micronuclei in PCEs. The results also show that a combination of Hibiscus sabdariffa and sodium arsenite reduced significantly the frequencies of micronucleated PCEs induced by sodium arsenite.     

西洋参提取物的毒理学试验研究

目的：为西洋参提取物安全性评价提供毒理学依据。方法：按卫生部《保健食品检验与评价技术规范》（2003年版）进行小鼠急性毒性试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验、Ames试验和大鼠30d喂养试验。结果：西洋参提取物对雌雄小鼠急性经口的最大耐受剂量（MTD）均大于10.0g/kgBW,属实际无毒级;三项致突变试验均未见致突变作用;大鼠30d喂养试验结果,3个剂量组大鼠各项指标与对照组比较均无显著性差异。结论：西洋参提取物属实际无毒级;未见潜在致突变作用;大鼠30d喂养试验,未见明显毒性反应。