SAG3基因在造血干细胞移植患者弓形虫感染诊断中的应用

目的探讨造血干细胞移植患者弓形虫感染的快速、特异、敏感的诊断方法。方法以弓形虫SAG3基因为靶基因，采用聚合酶链反应法(PCR)对56例造血干细胞移植受者及20名正常对照者进行检测，同时以酶联免疫吸附试验(ELISA)进行弓形虫抗体IgG、IgM的血清学检测。结果以PCR和ELISA检测弓形虫SAG3基因片段和其抗原，阳性分别为10例及7例，阳性率分别为17．9％和12．5％，其中PCR检测阳性、而ELISA检测阴性的3例，经病原学检测，均证实有弓形虫感染；20名正常对照者的检测结果均为阴性。结论体外扩增弓形虫SAG3基因的方法诊断弓形虫感染具有准确、快速、特异和敏感的优点，可作为造血干细胞移植患者弓形虫感染的诊断方法之一。     

SAG3基因在肾移植患者弓形虫感染诊断中的应用

目的:建立快速、特异、敏感诊断肾移植患者弓形虫感染情况的DNA检测方法,并讨论其临床意义.方法:利用ELISA和PCR方法对68例肾移植受者及20例正常供者同时进行检测.结果:检测肾移植受者68例,PCR和ELISA方法检测弓形虫SAG3基因片段和其抗原,阳性各为5例及4例;其阳性率分别为7.35%和5.91%.作为阴性对照的20名正常者同时进行弓形虫的检测,其结果均为阴性.结论:体外扩增弓形虫SAG3基因的方法具有准确、快速、特异和敏感的优点,可以作为肾移植患者弓形虫感染诊断的有价值的方法之一.     

男性不育精浆抗弓形虫抗体检测结果的140例临床分析

为了探讨弓形虫感染对男性生殖的影响，我们随机选择了140例男性不育病人，对其精浆进行了抗弓形虫抗体IgGIHA法检测和抗精子抗体（ASA）ELISA法检测。检测结果有38例抗弓形虫抗体阳性，阳性率为27％，明显高于我国正常人群的平均感染率．表明弓形虫感染可能是引起男性不育的一个因素，并对弓形虫感染引起男性不育的途径和发病机理进行了探讨。     

住院患儿弓形虫感染状况的调查及防护

对７８０例住院患儿进行三项弓形虫血清学指标（ＩｇＧ、ＩｇＭ及ＣＡｇ）检测。结果检出阳性率为７．１８％，同时报道各系统疾病中，以上三项弓形虫血清学指标阳性的分布情况及急性指标（ＩｇＭ、ＣＡｇ）阳性率情况，在鉴别和排除其他相应疾病的基础上，对弓形虫病患儿作出了早期诊断与及时治疗。从护理角度提出了重视预防，加强病情观察，防止患儿感染等措施，以降低患儿弓形虫感染阳性率及防止疾病发生。     

不孕症与弓形虫感染的检测意义

不孕症与弓形虫感染的检测意义杨迎工，韩胜凯，高虹，刘晓星关键词弓形虫病；不孕症近年来，随着对弓形虫病的逐渐认识，有关孕妇在妊娠期感染弓形虫，而弓形虫通过胎盘感染胎儿，造成流产、早产、死胎、畸形儿已有报道，但弓形虫病在不孕症病人中的感染情况，尚未引起重...     

实时荧光定量检测胃癌患者MUC1基因表达及临床意义

目的:检测胃癌患者肿瘤组织及外周血MUC1 mRNA的表达水平,探讨MUC1 mRNA表达的临床意义.方法:利用实时荧光定量PCR(RT-PCR)方法检测胃癌组织、正常组织及外周血中MUC1mRNA水平,分析其表达与肿瘤临床病理因素的关系.结果:胃癌患者肿瘤组织及外周血MUC1 mRNA表达水平高于正常对照组(P<0.05),肿瘤组织MUC1 mRNA的检出率与肿瘤的分化程度、淋巴结转移、血行转移及临床分期有关(P<0.05),外周血MUC1 mRNA的检出率与肿瘤的淋巴结转移、血行转移及临床分期有相关(P<0.05).结论:检测胃癌组织及外周血MUC1 mRNA的表达有助于早期发现胃癌及其微转移.     

男性不育精浆抗弓形虫抗体检测结果的140例临床分析

为了探讨弓形虫感染对男性生殖的影响，我们随机选择了１４０例男性不育病人，对其精浆进行了抗弓形虫抗体ＩｇＧＩＨＡ法检测和抗精子抗体（ＡＳＡ）ＥＬＩＳＡ法检测，检测结果有３８例抗弓形抗体阳性，阳性率为２７％，明显高于我国正常人群的平均感染率，表明弓形虫感染可能是引起男性不育的一个因素，并对弓形虫感染引起男性不育的途径和发病机理进行了探讨。     

在根治性前列腺切除术中用分子方法检测手术边缘

根治性前列腺切除术的手术边缘是否有肿瘤是估计预后的一个重要因素。常用方法仍然是传统的病理学检查，作者采用新的逆转录酶多聚酶链反应（ＲＴＰＣＲ）检测手术边缘是否有肿瘤，并与传统方法进行了对比。检测了３０例行前列腺根治性切除术的患者，于前列腺切除术后在前列腺窝和膀胱颈共取５处组织，每块组织约５ｍｍ×５ｍｍ×２ｍｍ，每块组织再分为２块，分别进行病理学检测和ＲＴＰＣＲ检测前列腺特异抗原及前列腺特异膜抗原。用无前列腺癌的前列腺切除标本作对照。从结果看，所有组织病理学边缘阳性或有包膜外侵犯的患者其分子检…     

CD133、肝细胞生长因子和垂体肿瘤转化基因在大肠癌中的表达及临床意义

目的：探讨CD133、肝细胞生长因子（hepatocyte growth factor,HGF）和垂体肿瘤转化基因（pituitary tumor transforming gene,PTTG）在大肠癌患者肿瘤组织、肿瘤旁正常组织和淋巴结中的表达及其与肿瘤患者临床之间的关系。方法应用免疫组化法检测70例大肠癌患者的肿瘤组织、肿瘤旁的正常组织和淋巴结,分别检测CD133、HGF、PTTG的表达情况。结果 CD133、HGF、PTTG三种蛋白均在肿瘤组织中高表达,且表达均与大肠癌的淋巴结转移及术后生存期有关（P＜0．05）,而与患者性别、年龄、肿瘤发生部位、临床病理分期及肿瘤大小无关（P＞0．05）;三种蛋白的表达两两之间无相关性。结论 CD133、HGF、PTTG三种蛋白对于大肠癌的诊断及鉴别诊断有一定的意义;三种蛋白的表达对大肠癌患者的术后复发、转移及其预后可能有指导意义。     

VEGF和微血管密度与大肠癌肝转移的关系

目的探讨血管内皮细胞生长因子（VEGF）和微血管密度（MVD）与大肠癌肝转移的关系。方法收集88例大肠癌患者的临床病理资料，检测VEGF血浆浓度、肿瘤组织含量，进行免疫组织化学染色，并计数微血管密度。结果血浆VEGF浓度与肝转移及TNM分期显著相关，肿瘤组织含量与肿瘤浸润深度、淋巴结转移、肝转移及TNM分期显著相关，VEGF染色阳性率与肝转移显著相关。VEGF和MVD正相关。术后肝转移患者的血浆VEGF浓度、肿瘤组织含量和染色阳性率以及MVD均显著高于无肝转移病例。结论VEGF和MVD与大肠癌的进展相关，是术后肝转移的预测指标。     

Transmission of toxoplasmosis by renal transplant

Two renal allograft recipients who had received their organs from the same cadaver donor developed acute toxoplasmosis shortly after transplantation. Neither of the recipients had serologic evidence of previous exposure to Toxoplasma gondii at the time of surgery, but the donor had a positive indirect fluorescent antibody test. One of the recipients died during the fourth week, and multiorgan involvement with toxoplasmosis was demonstrated at autopsy. No evidence of the parasite could be found in the transplanted kidney. In the second recipient the disease was suspected, serologically demonstrated, and successfully treated. We concluded that toxoplasmosis was transmitted by the donor's kidneys, although this mode of transmission was not completely proven.     

Hodgkin''s disease with a granulomatous pulmonary presentation mimicking sarcoidosis.

A prominent sarcoid like pulmonary granulomatous reaction to Hodgkin's disease was diagnosed six months before extrapulmonary Hodgkin's disease was confirmed histologically. It recurred with exacerbations of the lymphoma. The reaction is similar to that often seen at pathological staging of intra-abdominal organs not affected by Hodgkin's disease.     

Acute acquired toxoplasmosis presenting as polymyositis and chorioretinitis in immunocompetent patient

The parasite Toxoplasma gondii mainly encysts in brain, retina, myocardium, and skeletal muscle. It has been implicated in the genesis of inflammatory myopathies for years, but the parasite usually cannot be detected in the muscle. It is established, however, that toxoplasmosis can cause myositis either by recent infection or by reactivation. The case of a non-HIV patient who developed an acute polymyositis upon infection by T. gondii is reported. We suggest that all patients with polymyositis should have serological tests for toxoplasmosis as a part of their initial evaluation and early trial of antiprotozoal therapy in case of positive findings.     

Testicular toxoplasmosis: a rare case of a testicular mass

Toxoplasma gondii is an intracellular protozoan infecting birds and mammals. Acute infection is asymptomatic in immune competent people. For immune deficient patients (acquired immune deficiency syndrome, lymphoma patients or those under steroids to prevent organ transplantation rejection) infection may be lethal. We describe an uncommon case of testicular toxoplasmosis in patient under steroids after organ transplantation with no positive serum test for HIV and/or systemic toxoplasmosis.     

Primary intracerebral Hodgkin's disease mimicking meningioma: case report

OBJECTIVE AND IMPORTANCE: A rare case of dura-based primary cerebral Hodgkin's disease clinically and radiographically indistinguishable from a meningioma is described. CLINICAL PRESENTATION: A 55-year-old immunocompetent woman presented with headaches and ataxia. Magnetic resonance images demonstrated a circumscribed diffusely enhancing mass with a dural tail attached to the cerebellar tentorium. INTERVENTION: Operative inspection also suggested a meningioma, but a frozen section of the firm mass revealed an inflammatory lesion. Subsequent pathological analysis demonstrated Hodgkin's disease, nodular sclerosing type. An extensive workup revealed no systemic disease. CONCLUSION: This case illustrates the rare occurrence of primary intracranial Hodgkin's disease and its mimicry of meningioma.     

Toxoplasmosis of the conus medullaris in a patient with hemophilia A-associated AIDS. Case report

Toxoplasma gondii has been reported to be the most common cause of focal brain lesions in patients with acquired immunodeficiency syndrome (AIDS). A case of intramedullary toxoplasmosis of the conus medullaris is reported in a patient with hemophilia A-associated AIDS. The diagnosis is discussed, with particular emphasis on the magnetic resonance imaging appearance.     

Childhood toxoplasmosis today

The comparative study of two groups of children suffering from acquired or congenital toxoplasmosis, being in hospital during two different decades (1979-1985--39 children and 1991-1997--117 children) proved: the fact that atypical or subclinical manifestations of the acquired toxoplasmosis (54%) draws our attention to the systematic research of the infection with Toxoplasma gondii, especially at the groups with increased risk now that the frequency of the diseases generating immunodeficiency; the increased frequency of congenital toxoplasmosis (5% in the first group, 8% in the second group) and especially of the invalidating of screening tests to the pregnant women; the therapeutic arsenal limited by the rate price/toxicity/medical resistance, must be individualized for each case, taking into account the increased number of other associated diseases (chronic hepatitis B 7%, TB 17%, HIV infection 5%); Rovamicine treatment at the second group had similar results to those obtained by administrating Pirimetamine--Trimetoprim to the children from the first group.     

Toxoplasmic pneumonitis leading to fatal acute respiratory distress syndrome after engraftment in three bone marrow transplant recipients.

Toxoplasmosis is a rare but severe complication of bone marrow transplantation. Here, we report three patients in whom toxoplasmic pneumonitis developed, leading to fatal acute respiratory distress syndrome (ARDS).All patients had positive pretransplantation tests for Toxoplasma gondii and were therefore at risk to develop toxoplasmosis reactivation. They all recovered from aplasia, but soon after they died from brutal and severe ARDS. The possible role of an immunopathologic response to T gondii in the lungs in triggering ARDS is discussed.Early screening of parasitemia using highly sensitive polymerase chain reaction methods in seropositive patients with unexplained fever may be needed.     

Staging laparotomy in early Hodgkin's disease: a surgical view

Fifty consecutive patients with early Hodgkin's disease underwent staging laparotomy. There were no deaths and most complications were minor. As a consequence, 17 (34%) patients were upstaged from clinical stage I or II to pathological stage III or IV. Subdiaphragmatic disease was found in five out of 22 patients with nodular sclerosing, seven out of ten with lymphocyte predominant and four out of 13 patients with mixed cellularity histological type. The age, sex, preoperative clinical stage and mediastinal involvement did not correlate with the presence of intra-abdominal disease. Among 15 patients with a normal preoperative computed tomography (CT) scan, nine had intra-abdominal disease at laparotomy and all three patients with suggested subdiaphragmatic involvement by the CT scan examination were clear. The surgeon's ability to diagnose early involvement at laparotomy was also limited. There seems to be a continued need for staging laparotomy to ensure adequate treatment of one-third of patients with early Hodgkin's disease.     

Spinal toxoplasmic arachnoiditis associated with osteoid formation: a rare presentation of toxoplasmosis

STUDY DESIGN: An extremely rare presentation of an isolated spinal toxoplasmic arachnoiditis is described. OBJECTIVE: To draw attention to the fact that spinal arachnoid membranes may be a potential reservoir for Toxoplasma gondii. SUMMARY OF BACKGROUND DATA: Central nervous system toxoplasmosis is a common manifestation in patients who are immunodeficient. Reports on the spinal toxoplasmosis are rare and focused on spinal cord involvement. METHODS: An adult patient presented with symptoms of spastic paraparesis that had begun 13 years before admission. Thoracic spinal magnetic resonance imaging showed small lesions in posterior subarachnoid space at Th7-Th8. A Th7-Th8 laminectomy was performed. Intradural-extramedullary lesions were excised. RESULTS: Clinical, immunologic, and pathologic examinations showed adhesive spinal arachnoiditis associated with osteoid formation caused by past toxoplasmic infection. There was no impairment of the immunologic defense system. CONCLUSION: Where no causative factor is found in serious spinal adhesive arachnoiditis, the possibility of spinal toxoplasmosis should also be investigated.