肺结核病患者与HLA-DRB1等位基因的关联

目的:通过对湖北省汉族肺结核病(PTB)患者进行HLA-DRB1基因分型,探讨此人群与HLADRB1的相关性。方法:用序列特异性寡核苷酸探针聚合酶链反应(PCR-SSOP)技术对174例PTB患者及1 358例健康人群进行HLA-DRB1基因分型。基因频率与Hardy-Weinberg平衡卡方检测使用arlequin软件进行,组间比较用Fisher精确概率法计算P值,检测水准为a=0.05,相对危险系数用RR表示,RR=A(A+B)/C(C+D)。结果:174例PTB患者HLA-DRB1基因分型结果与希望值进行H-W平衡检测(P0.05)。此人群检出HLA-DRB1等位基因共13种与对照组一致。其中RB1*08与DRB1*09基因频率显著高于对照组,差异有统计学意义(P0.01),且RR1,说明DRB1*08与DRB1*09或与其连锁的单倍型可能是的PTB易感基因;RB1*10与DRB1*13基因频率显著低于对照组,差异有统计学意义(P0.05),且RR1,说明RB1*10与DRB1*13可能是PTB的保护基因。其余等位基因频率组间比较均无统计学意义。结论:湖北省汉族PTB患者这个疾病群体遗传平衡被杂化,其与HLA-DRB1有一定的关联,但有其自身的特点。     

Graves病白细胞减少易感性与HLA-DRB1基因多态性的关联

目的探讨天津地区汉族Graves病(GD)白细胞减少易感性与HLA-DRB1基因多态性的关联.方法采用聚合酶链反应-序列特异性引物(PCR-SSP)方法检测45例GD白细胞减少患者、50例GD白细胞正常患者和90名正常对照的HLA-DRB1等位基因频率.结果(1)在不考虑白细胞变化的情况下,GD患者DRB1*08基因频率明显高于对照组(P＜0.01,RR=2.62),DRB1*07基因频率明显低于对照组(P＜0.01,RR=0.24).(2)GD白细胞减少组DRB1*08(P＜0.01,RR=4.17)和DRB1*15(P＜0.05,RR=1.69)基因频率较对照组显著增高,DRB1*07基因频率明显低于对照组(P＜0.01,RR=0.13).(3)GD白细胞减少组DRB1*08基因频率(P＜0.01)和DRB1*15基因频率(P＜0.05)均明显高于白细胞正常组,DRB1*09(P＜0.05)基因频率明显低于白细胞正常组.结论天津地区汉族GD白细胞减少易感性与HLA-DRB1*08,HLA-DRB1*15基因频率增加有关;GD的保护性与HLA-DRB1*07基因频率减少有关.     

应用基因芯片分析HLA-DRB与晚期肝脾型日本血吸虫病的相关性

目的 探讨人类白细胞抗原Ⅱ类(HLA-Ⅱ）基因多态性与晚期肝脾型日本血吸虫病的遗传关联性。 方法 应用基因芯片分析技术对武汉市蔡甸45例晚期肝脾型日本血吸虫病患者（实验组）和44例慢性日本血吸虫病患者（对照组）的HLA-Ⅱ基因DRB位点等位基因进行基因分型，并比较两组各等位基因频率以及与晚期肝脾型日本血吸虫病的相关性。 结果 实验组HLA-DRB1*04x等位基因频率明显高于对照组（P＜0.01， RR=3.928），而对照组HLA-DRB1*15x等位基因频率明显高于实验组（P＜0.01， RR=0.050）。等位基因DRB1*15x总与DRB5*010x/020x连锁，对照组DRB1*15x-DRB5*010x/020x连锁体频率明显高于实验组（P＜0.01）。 结论 HLA-DRB1*04x与晚期肝脾型日本血吸虫病呈正相关，而HLA-DRB1*15x与晚期血吸虫病呈负相关。     

新疆维吾尔族人群HLA-DR、DQ基因多态性与结核病易感性的研究

目的 探讨人类白细胞抗原(HLA)-DRB1、DQB1基因多态性与新疆维吾尔族人群结核病易感性的关联。 方法 采用病例-对照的研究方法,应用聚合酶链反应-序列特异性引物(PCR-SSP）技术对226例新疆维吾尔族肺结核病患者(肺结核病例组)和231例新疆维吾尔族健康对照者(健康对照组)进行HLA-DRB1、DQB1基因分型,比较其等位基因频率(GF),并计算其比值比(OR)。 结果 1. 肺结核病例组中HLA-DRB1*11基因频率显著高于健康对照组,2组的GF分别为4.5%、1.1%,差异有统计学意义(OR=4.388,95%CI=1.618～11.905,P_c＜0.05);肺结核病例组中HLA-DRB1*04基因频率显著高于健康对照组,2组的GF分别为12.8%、8.4%,但P值经过校正后差异无统计学意义(OR=1.686,95%CI=1.060～2.684,P_c>0.05)。 2. 肺结核病例组中HLA-DQB1*0201基因频率显著高于健康对照组,2组的GF分别为40.1%、19.2%,差异有统计学意义(OR=3.379,95%CI=2.302～4.960,P_c＜0.05); 肺结核病例组中HLA-DQB1*0301/4基因频率显著低于健康对照组,2组的GF分别为6.2%、10.3%,但P值经过校正后差异无统计学意义(OR=0.561、95%CI=0.334～0.941,P_c＞0.05)。 结论 HLA-DRB1*11、DQB1*0201等位基因与新疆维吾尔族人群结核病强相关,DRB1*11、DQB1*0201可能是其易感基因。     

肺结核病患者与HLA-DRBI等位基因的关联

目的：通过对湖北省汉族肺结核病（PTB）患者进行HLA-DRB1基因分型,探讨此人群与HLA-DRB1的相关性。方法：用序列特异性寡核苷酸探针聚合酶链反应（PCR-SSOP）技术对174例PTB患者及1358例健康人群进行HLA-DRB1基因分型。基因频率与Hardy-Weinberg平衡卡方检测使用arlequin软件进行,组间比较用Fisher精确概率法计算P值,检测水准为a=0．05,相对危险系数用RR表示,RR：A（A＋B）／C（C＋D）。结果：174例PTB患者HLA-DRB1基因分型结果与希望值进行H-W平衡检测（P〉0．05）。此人群检出HLA-DRB1等位基因共13种与对照组一致。其中RB1＊08与DRB1＊09基因频率显著高于对照组,差异有统计学意义（P〈O．01）,且RR〉1,说明DRB1＊08与DRB1＊09或与其连锁的单倍型可能是的PTB易感基因;RB1＊10与DRB1＊13基因频率显著低于对照组,差异有统计学意义（P〈0．05）,且RR〈1,说明RB1＊10与DRB1＊13可能是PTB的保护基因。其余等位基因频率组间比较均无统计学意义。结论：湖北省汉族PTB患者这个疾病群体遗传平衡被杂化,其与HLA-DRBl有一定的关联,但有其自身的特点。     

山东地区汉族骨髓捐献者HLA-DRB1基因分型及多态性分析

目的观察山东地区汉族人群人白细胞抗原(HLA)-DRB1基因多态性及其分布特点。方法采用聚合酶链反应—测序为基础的分型方法(PCR-SBT)对随机抽取的909例中华骨髓库山东分库内山东汉族骨髓供者血样进行HLA-DRB1基因分型和多态性分析。结果共检测出42种HLA-DRB1等位基因,其中HLA-DRB1*0901等位基因频率最高(16.28%),其次为HLA-DRB1*1501(15.13%)和HLA-DRB1*0701(14.04%),基因频率最低的是DRB1*0408、DRB1*0809、DRB1*1103、DRB1*1303、DRB1*1412、DRB1*1425、DRB1*1601,各占0.06%。山东汉族骨髓供者HLA-DRB1等位基因的分布与江苏、湖北、新疆、广东、韩国、日本和德国人群存在明显差异(P均<0.05)。结论掌握了山东地区汉族人群HLA-DRB1基因多态性及其分布特征。     

HLA-DRB1和肿瘤坏死因子α基因多态性与肝硬化的遗传易感性

目的探讨HLA-DRB1和肿瘤坏死因子(TNF)α基因多态性与肝硬化遗传易感性之间的关系.方法应用聚合酶链反应-序列特异性引物法、限制性片段长度多态性等技术检测106例乙型肝炎后肝硬化患者和108例健康对照者的HLA-DRB1和TNFα基因多态性.结果肝硬化组HLA-DRB1*120X等位基因频率比对照组显著升高(35.9%比11.1%,P＜0.001),TNFα中TNF2/1基因型频率比对照组明显升高(19.8%比10.2%, P＜0.05),DRB1*150X等位基因频率明显低于对照组 (13.2%比30.6% ,P＜0.05),分层分析表明,DRB1*120X等位基因与肝硬化的关联大于TNF2等位基因.结论 HLA-DRB1*120X和TNF2等位基因与乙型肝炎后肝硬化的遗传易感性相关,携带这2个等位基因的个体发生肝硬化的危险性增加.HLA-DRB1*120X等位基因可能是肝硬化的易感基因,HLA-DRB1*150X等位基因为抗性基因.     

抗甲状腺药物致白细胞减少易感性与HLA-DRB1基因多态性及ANCA的关联性

目的 研究安徽地区汉族Graves病患者使用抗甲状腺药物(ATD)致白细胞减少的易感性与HLA-DRB1基因多态性及抗中性粒细胞胞浆抗体(ANCA)的相关性.方法 采用聚合酶链反应-序列特异性引物方法(PCR-SSP)检测76例ATD致白细胞减少的Graves病患者、98例ATD治疗后白细胞正常患者和230名健康对照者的等位基因HLA-DRB1* 08032、DRBI* 1501、DRB1*0901的频率.采用间接免疫荧光法(IIF)检测白细胞减少组和白细胞正常组Graves病患者的血清ANCA阳性率.结果 (1)与健康对照组及细胞正常组比较,白细胞减少组患者等位基因DRB1 * 08032、DRB1*1501频率明显增加(OR分别为3.06,1.77,4.03和2.28,均P＜0.05),DRB1* 0901频率明显减低(OR为0.33和0.43,P＜0.05).(2)与甲巯咪唑治疗后白细胞正常组患者比较,甲巯咪唑致白细胞减少组患者血清ANCA阳性率明显增加(x2 =4.878,P＜0.05).(3)与未携带等位基因DRB1*08032和DRB1*1501的患者比较,携带者血清中ANCA阳性率明显增加(x2为5.682,5.429,4.009和4.549,均P＜0.05).结论 等位基因HLA-DRB1*08032、HLA-DRB1*1501可能是安徽地区汉族人ATD致白细胞减少的易感基因;HLA-DRB1*0901可能是其保护基因或抗性基因.免疫反应可能参与了ATD致白细胞减少的发生.免疫反应的发生可能存在遗传易感性.     

HLA-DRB1基因多态性与新疆维吾尔族人群结核病易感性的相关性研究

目的探讨人类白细胞抗原(HLA)-DRB1基因多态性与新疆维吾尔族人群结核病易感性的关联。方法采用病例-对照方法 ,应用聚合酶链反应-序列特异性引物(PCR-SSP)技术对226例新疆维吾尔族肺结核病患者(肺结核病例组)和当地同族231人健康者进行HLA-DRB1基因分型,比较其等位基因频率(GF),并计算其比值比(OR)。结果肺结核病例组中HLA-DRB1*11基因频率显著高于健康对照组,两组的GF分别为4.53%和1.09%,差异有统计学意义(OR=4.388,χ2=9.872,Pc=0.026<0.05);肺结核病例组中HLA-DRB1*04基因频率亦显著高于健康对照组,两组的GF分别为12.77%和8.36%,但P值经过校正后差异无统计学意义(Pc>0.05)。结论 HLA-DRB1*11等位基因与新疆维吾尔族人群结核病呈相关性。     

汉族老年慢性乙型肝炎患者HLA-DRB1基因多态性

目的探讨汉族老年慢性乙型肝炎患者HLA-DRB1基因多态性。方法选取汉族老年人群中的53例慢性乙型肝炎患者、100例正常健康老年人以及31例乙型肝炎表面抗原携带者作为研究对象,分别为病例组、抗原携带组和健康对照组,对三组研究对象的HLA-DRB1等位基因分型进行比较,并对HBV不同复制状态者HLA-DRB1等位基因分型进行分析。结果病例组的HLA-DRB1*03等位基因频率明显较健康对照组高,而HLA-DRB1*15等位基因频率明显低于健康对照组(P<0.05),抗原携带者与健康对照组比较,HLA-DRB1各等位基因的频率,无显著性差异(P>0.05)。HBV高复制状态组的DRB1*07基因位点明显较低复制状态组多见(P<0.05)。结论汉族老年慢性乙型肝炎患者与HLA-DRB1基因多态性之间存在比较强的相关性。     

HLA-DRB3*0101 is associated with Graves' disease in Jamaicans

OBJECTIVES: Graves' disease is associated with different human leucocyte antigen (HLA) genes in different populations. This studywasdesigned to examinethe HLA class II associations with Graves' disease in Jamaicans. PATIENTS: One hundred and six Jamaicans with Graves' disease and 104 controls. DESIGN: Oligotyping for HLA-DRB1, DRB3, DQA1 and DQB1 alleles was performed using the polymerase chain reaction sequence specific oligonucleotide probe (PCR-SSOP) technique. RESULTS The frequency of HLA-DRB3 *0101 was increased significantly in the patients compared to controls (38.7% vs. 19.2%; RR = 2.72; Pc < 0.015). The protective alleles for Graves' disease were DRB1 *0901 (0.9% vs. 20.2%; RR = 0.04; Pc < 0.001), DRB1*1001 (0.0% vs. 11%; RR = 0.0%; Pc < 0.01) and DRB4 *0101 (0.0% vs. 12.5%; RR = 0.0; Pc < 0.05). A high female to male ratio of Graves' disease, 25 :1, was observed. Other associated autoimmune diseases were rare and no significant HLA class II associations were found with clinical markers of disease. CONCLUSIONS: Jamaican patients with Graves' disease share the DRB3 *0101 susceptible allele and the DRB4 *01 protective allele but not the susceptible haplotype DRB1 *0301, DRB3*0101, DQA1*0501 with Caucasians.     

人类白细胞抗原—DRB1和DQB1基因与肺结核合并糖尿病的相关性分析

目的 探讨人类白细胞抗原（HLA）－DRB1手DQB1基因与肺结核合并2型糖尿病的关联性;寻找与肺结核合并2型糖尿病可能相关的HLA基因。方法 采用病例对照和聚合酶链反应－特异性序列引物（PCR－SSP)方法,对我国北方汉族123例肺结核合并2型糖尿病患者和与其无因缘关系的46名健康对照以及45全单纯2型糖尿病患者分别进行HLA－DRB1和DQB1闰点的等位基因分型。结果 肺结核合并2型糖尿病患者组中DRB1＊09基因频率明显高于健康人组,分别为25．10％和14．03％,RR为2．22,肺结核合并2型糖尿病患者组中DRB1＊09基因频率明显高于单纯2型糖尿病患者组,分别为25．10％和9．32％,RR为3．16,统计学上差异均有显著性;在肺结核合并Ⅱ型糖尿病患者组中DQB1＊05基因频率明显低于单纯2型糖尿病患者组,分别为7．17％和21．12％,RR为0．26,统计学差异有非常显著性。结论 研究提示DRB1＊09基因可能是肺结核合并2型糖尿病的易感基因;DQB1＊05基因可能是肺结核合并2型糖尿病的保护基因;可以推测DRB1＊09和DQB1＊05基因在肺结核合并2型糖尿病的发病中起一定作用,或是真正起作用的基因与它们连锁,有待于进一步研究。     

大动脉炎易感性与HLA-DRB基因相关性研究

目的 探讨大动脉炎易感性与ＨＬＡ－ＤＲＢ等位基因频率的相关性。方法 以８４例汉族大动脉炎患和１０２例健康汉族人（对照组）为研究对象,采用ＰＣＲ－ＳＳＰ及ＰＣＲ－ＲＦＬＰ方法进行ＨＬＡ－ＤＲＢ等位基因分型,比较其等位基因频率在大动脉炎患与对照组之间的差异。结果 ＨＬＡ－ＤＲ４、ＤＲ７等位基因频率在大动脉炎组显高于对照组（３８．１％比１５．７％,Ｐ〈０．０１,ＲＲ＝２．４３;４７．６％比１０．８     

Relationship between human leukocyte antigen-DRB1 and autoimmune hepatitis type I in Chinese patients

AIM: To analyze the association of human leukocyte antigen (HLA)-DRB1 with autoimmune hepatitis type I (AIH) among Chinese patients in the Shanghai area. METHODS: In 32 patients and 48 healthy controls, polymerase chain reaction amplified with sequence-specific primers (PCR-SSP) was performed to elucidate the relevance of certain alleles or polymorphic sequences of HLA-DRB1 with autoimmune hepatitis. RESULTS: The HLA-DRB1 typing by PCR-SSP showed that DR4 had a significantly increased frequency among patients with AIH versus that of healthy controls (46.9 vs 20.8%; relative risk = 3.35, P = 0.014). In the subtypes of DR4, there was a trend of an increase in the gene frequency of DRB1*0405 in patients with AIH versus that of healthy controls (21.9 vs 6.3%, P = 0.04, but corrected P (Pc) = 0.08). In addition, our analysis indicated a significant increase in the alleles frequency encoding Leu-Leu-Glu-Gln-Lys-Arg (LLEQRR) from the third hyperpolymorphic region (HVR3) of DR4 in the patients with AIH (86.7% of DR4 positive patients vs 40.0% in DR4 positive controls, P = 0.016, Pc = 0.028, relative risk (RR) = 9.75). CONCLUSION: Type I AIH among Chinese patients is associated with HLA-DR4. There is a relevance of type I AIH and LLEQRR sequence within the third hyperpolymorphic region of the DRB1 allele.     

Human leukocyte antigen allele associations in type-1 autoimmune hepatitis patients from western India

BACKGROUND AND AIMS: The immunogenetic basis of autoimmune diseases has become more and more evident. We have analyzed the human leukocyte antigen (HLA) associations with type-1 autoimmune hepatitis (AIH) among patients from western India. METHODS: In 20 patients and 120 healthy controls, polymerase chain reaction amplified with sequence specific primers and hybridized with oligoprobes was carried out to elucidate the HLA A, B, C and DRB1 allele associations. RESULTS: The study revealed that A*0222 (20% vs 1.66%; P = 0.0001), A*3201 (15% vs 0.83%; P = 0.0004), A*680102 (30% vs 6.66%; P = 0.001), B*35 (40% vs 11.66%; P = 0.001), B*5501 (10% vs 0.83%; P = 0.008), Cw*0102 (15% vs 1.66%; P = 0.002) and Cw*070101 (50% vs 11.66%; P = 2.5E-05) were significantly increased among the A, B and C alleles of AIH patients. Among the HLA DRB1 alleles, DRB1*0301 (20% vs 6.19%; P = 0.03), DRB1*1301 (15% vs 2.65%; P = 0.01), DRB1*14 (30% vs 11.5%; P = 0.02) and DRB1*1501 (40% vs 22.12%; P = 0.08) were increased in AIH patients when compared with the controls. CONCLUSIONS: The present study indicates that the HLA susceptibility to type 1 AIH in the different populations studied is complex.     

Association of the HLA-DRB1 gene with susceptibility to aortoarteritis in a Chinese Han population.

Aortoarteritis is a chronic inflammatory disease mainly affecting the aorta and its major branches. Recent immunogenetic studies indicate that certain human leucocyte antigen (HLA) alleles are significantly associated with aortoarteritis in several populations. The purpose of the present study was to investigate the relationship between the HLA-DRB1 alleles and aortoarteritis in a Chinese Han population. HLA-DRB1 genotypes were identified by PCR-SSP and PCR-RFLP in 84 Chinese patients with aortoarteritis and 102 healthy Chinese controls. It was found that the HLA-DRB1*04 allele (38.1% in patients vs. 15.7% in controls, p<0.001, relative risk (RR)=2.43) and the HLA-DRB1*07 allele (47.6% vs. 10.8%, p<0.001, RR = 4.42) were significantly associated with aortoarteritis. Furthermore, there was no significant difference in the frequency of the DRB1*0405 subtype between the patient and control groups. Thus the susceptibility to aortoarteritis in this Chinese Han population was closely related with the HLA-DRB1*04 and DRB1*07 alleles. Thus individuals with the HLA-DRB1*04 and DRB1*07 alleles may be at higher risk for developing aortoarteritis.     

HLA-DRB1、-DQB1基因多态性与食管鳞癌遗传关联性

目的　从基因水平探讨食管鳞癌HLA DRB1 , DQB1等位基因的遗传易感性 ,以阐述其免疫遗传学特征。方法　运用序列特异性引物聚合酶链反应技术 ,检测无亲缘关系湖北汉族健康人 1 36例、食管鳞癌患者 42例的HLA DRB1 , DQB1等位基因。结果　湖北汉族人食管鳞癌患者与正常人比较 ,HLA DRB1 0 90 1等位基因分布频率显著增高 (0 .2 50 0比 0 .1 397,P =0 .0 2 8,OR =2 .0 53 ,病因分数 =0 .1 2 82 ) ,HLA DQB1 0 30 1基因分布频率显著增高 (0 .2 976比 0 .1 875 ,P =0 .0 4 6 ,OR =1 .835 ,病因分数 =0 .1 35 4)。两者间其余HLA DRB1、 DQB1等位基因分布频率差异均无显著性。结论　HLA DRB1 0 90 1及 DQB1 0 30 1等位基因均与食管鳞癌正关联 ,为其易感基因。该两等位基因测序结果与其基因库第 2外显子序列吻合。     

Molecular subtypes of the HLA-DR antigens in pulmonary tuberculosis.

OBJECTIVE: The aim of this study was to analyze association between HLA-DRB1 alleles and pulmonary tuberculosis (PTB) in the Polish population. METHODS: The HLA-DRB1 typing was performed using sequence-specific amplification (polymerase chain reaction with sequence specific primer [PCR-SSP] in 31 patients and 58 healthy volunteers. The DRB1 primers were supplied by DYNAL in the standard kit DYNAL DR "low-resolution"-SSP. RESULTS: The study showed that the DRB1*16 alleles frequency was higher in patients with PTB than in the tested group of healthy controls (P < 0.01). When HLA-DR2 alleles were combined (i.e., the DRB1*15 with DRB1*16 alleles), their frequency was comparable with that in the healthy individuals. The highest relative risk (RR) of tuberculosis was associated with DRB1*16 alleles (RR = 9.7). When HLA-DR6 alleles were combined (i.e., the DRB1*13 with DRB1*14 alleles), only a trend for higher frequency in patients with PTB was found. Frequency of DRB1*13 alleles of HLA-DR6 was significantly lower in PTB than in the healthy individuals (P < 0.001; RR = 0.04). CONCLUSIONS: Results suggest that the presence of HLA-DRB1*16 alleles may increase the risk of development of PTB, whereas HLA-DRB1*13 alleles may be resistant to tuberculosis.     

Association of HLA Class II Genes with Idiopathic Pulmonary Arterial Hypertension in Koreans

The aim of this study was to compare the frequency of the HLA-DRB1 and HLA-DQB1 alleles in Korean patients with idiopathic pulmonary arterial hypertension (IPAH) and in normal controls and to determine any association that may exist between clinical characteristics of IPAH and specific HLA alleles. IPAH patients seen between October 1998 and September 2001 were retrospectively assessed, and 19 patients and 193 controls were HLA typed at the HLA-DRB1 and DQB1 loci. Clinical characteristics and hemodynamic parameters were reviewed. The patients with IPAH had a significantly higher frequency of the HLA-DRB1*0406 allele (18% vs. 6%, p = 0.004) and the HLA-DQB1*0302 allele (24% vs. 12%, p = 0.034), as well as a significantly higher frequency of haplotype DRB1*0406-DQB1*0302 (p = 0.0006). All 6 patients with haplotype DRB1*0406-DQB1*0302 (H+ group) were women, compared with 8 of the 13 patients lacking the DRB1*0406-DQB1*0302 haplotype (H- group), but without statistical significance. Three of 19 patients showed a positive short-term hemodynamic response to NO inhalation, all 3 of whom had the DRB1*0406-DQB1*0302 haplotype. There were no other significant differences in clinical characteristics and hemodynamic parameters between the H+ and H- groups. We conclude from this study that the HLA-DRB1*0406-DQB1*0302 haplotype is associated with IPAH in Korean patients. These results suggest that certain clinical characteristics of IPAH may be controlled in part by patients' HLA alleles.     

Association between HLA class Ⅱ gene and susceptibility or resistance to chronic hepatitis B

AIM: To investigate the association between the polymorphism of HLA-DRB1, -DQA1 and -DQB1 alleles and viral hepatitis B. METHODS: HLA-DRB1, -DQA1 and -DQB1 alleles in 54 patients with chronic hepatitis B, 30 patients with acute hepatitis B and 106 normal control subjects were analyzed by using the polymerase chain reaction/sequence specific primer (PCR/SSP) technique. RESULTS: The allele frequency of HLA-DRB1*0301 in the chronic hepatitis B group was markedly higher than that in the normal control group (17.31% vs 5.67 %), there was a significant correlation between them (X^2= 12.3068,PC=0.0074, RR=4.15). The allele frequency of HLA-DQAI*0501 in the chronic hepatitis B group was significantly higher than that in the normal control group (25.96 % vs 13.68 %), there was a significant correlation between them (X^2=9.2002, PC=0.0157, RR=2.87). The allele frequency of HLA-DQBI*0301 in the chronic hepatitis B group was notably higher than that in the normal control group (35.58 % vs 18.87 %), there was a significant correlation between them (x^2=15.5938, PC=0.0075, RR=4.07). The allele frequency of HLA-DRB1*1101/1104 in the chronic hepatitis B group was obviously lower than that in the normal control group (0.96 % vs 13.33 %), there was a significant correlation between them (X^2=11.9206, PC=0.0145, RR=18.55). The allele frequency of HLA-DQAI*0301 in the chronic hepatitis B group was remarkably lower than that in the normal control group (14.42 % vs30 %), there was a significant correlation between them (X^2=8.7396, PC=0.0167, RR=0.35). CONCLUSION: HLA-DRBI*0301, HLA-DQAI*0501 and HLA-DQBI*0301 are closely related with susceptibility to chronic hepatitis B, and HLA-DRB1*1101/1104 and HLA-DQAI*0301 are closely related with resistance to chronic hepatitis B. These findings suggest that host HLA class Ⅱ gene is an important factor determining the outcome of HBV infection.