精子空泡对ICSI患者胚胎发育及临床结局的影响

目的利用精子放大系统观察精子空泡位置和大小,探讨不同类型精子行卵胞浆内单精子注射(ICSI)后对患者胚胎发育及临床结局的影响。方法选取57例行ICSI患者,于注射前利用精子放大系统对已在低倍镜下选定的精子进行放大观察,并拍照测量空泡直径,最后注射入卵,注射后单独培养并观察受精结局。结果空泡组和无空泡组比较,除胚胎冷冻率(10.6%vs.25.0%)和临床妊娠率(22.7%vs.77.3%)有显著性差异(P0.05)外,两组的2PN率分别为71.4%、74.1%,D3优胚率为28.3%、33.2%,可用囊胚率为11.1%、23.8%,胚胎利用率为44.6%、47.3%,胚胎着床率为22.2%、40.3%,两组间比较均无显著性差异(P0.05);同时比较空泡组中空泡位置(顶体或顶体+核)对临床结局的影响,结果显示正常受精率分别为75.7%、44.4%,D3优胚率为34.7%、0%,可用囊胚率为7.1%、12.5%,胚胎冷冻率为13.3%、12.5%,胚胎利用率为49.0%、25.0%,空泡组空泡位置间受精结局亦均无显著性差异(P0.05);观察精子空泡大小(直径:1μm、1~1.5μm、1.5~2μm)对ICSI结局影响,结果显示3组正常受精率分别为71.0%、68.0%、76.9%,D3优胚率为25.0%、30.0%、54.5%,可用囊胚率为7.7%、7.1%、20.0%,胚胎冷冻率为14.3%、35.7%、20.0%,胚胎利用率为29.4%、40.0%、63.6%,3组间比较均无显著性差异(P0.05)。结论精子空泡有无对胚胎发育和胚胎着床并无显著影响,但因本研究样本量较少、精子空泡直径较小,故后期需扩大样本量来进一步验证。本结果仅为精子空泡的相关研究提供一定参考。     

严重少弱畸精子症患者精液和睾丸中精子的ICSI临床结局比较

目的 探讨睾丸取精能否改善严重少弱畸精子症的男性不育患者行卵胞浆内单精子注射(ICSI)的临床结局。方法 回顾性分析2016年1月至2020年6月因男性严重少弱畸形精子症于保定市妇幼保健院生殖医学科行ICSI助孕的58对夫妇的临床资料。按照获取精子的方式不同,将患者分为两组：射出精子ICSI组(E-ICSI组,56个周期)和睾丸精子ICSI组(T-ICSI组,42个周期)。比较两组患者的基础资料、促排卵情况、授精及胚胎发育情况和临床妊娠结局的差异。结果 58对夫妇共接受了98个ICSI周期,56个周期(57.14%)使用射精精子,42个周期(42.86%)使用睾丸精子。两组患者的基础资料、促排卵方案、Gn用量、HCG日E₂及P水平、成熟卵数、获卵数比较,均无统计学差异(P>0.05)。E-ICSI组与T-ICSI组的2PN受精率(56.4%vs. 53.4%)、优质胚胎率(8.5%vs. 6.1%)、卵母细胞利用率(25.9%vs. 24.2%)均无统计学差异(P>0.05)。E-ICSI组与T-ICSI组的新鲜周期临床妊娠率(22.7%vs. 27....     

多周期IVF-ET患者新鲜移植与冻融胚胎移植妊娠结局的比较

目的比较多周期IVF/ICSI-ET患者新鲜移植与第1次冻融胚胎移植(FET)的妊娠结局。方法回顾性分析2014年1月至2018年12月在我院生殖中心行2个周期以上的IVF/ICSI-ET助孕周期患者(共2 155个周期)的临床资料。根据是否行新鲜周期移植分为2组:新鲜周期移植组,共1 474个周期;FET组,共681个周期。比较2组患者的基本资料、促排卵实验室指标及妊娠结局,并采用Logistic回归分析活产率的影响因素。结果两组患者间原发不孕比例、体重指数(BMI)比较无显著性差异(P>0.05);FET组患者年龄、周期次数、不孕年限均显著高于新鲜周期组(P<0.05)。新鲜周期组长方案、超长方案、超短方案占比均显著大于FET组(P<0.05);FET组拮抗剂方案占比显著大于新鲜周期组(P<0.05)。新鲜周期组Gn天数、移植前末次内膜厚度、获卵数均显著大于FET组(P<0.05);FET组HCG日孕酮(P)水平显著高于新鲜周期组(P<0.05)。新鲜周期组的活产率、早产率、双胎率均略高于FET组,但尚无显著性差异(P>0.05)。行多因素...     

梗阻性无精子症患者睾丸抽吸精子行ICSI后的胚胎结局

目的总结非手术精子抽吸(NSA)联合ICSI对梗阻性无精子症患者的治疗意义,并探讨精子来源等对ICSI后胚胎结局的影响。方法回顾性分析642个ICSI周期,比较了睾丸抽吸精子和精液精子行ICSI后的受精率、优质胚胎率、临床妊娠率、种植率及胚胎停育率等。结果NSA睾丸精子组和精液精子组相比较,其2PN受精率、优质胚胎率、临床妊娠率、种植率及胚胎停育率之间均没有显著性差异(71．4％vs 73．2％, 73．1％vs 70．1％,34．2％vs 32．6％,22．3％vs 19．8％,21．3％vs 25．0％,P>0．05)。结论非手术抽吸睾丸精子联合ICSI是治疗梗阻性无精子症的一种有效方法。     

卵母细胞成熟率与ICSI临床结局的关系分析

目的探讨卵母细胞成熟率与卵胞浆内单精子注射(ICSI)临床结局的关系。方法回顾性分析2014年1~12月在本中心接受ICSI治疗且获卵数≥5枚、卵母细胞成熟率≥30%的2 196个周期,根据卵母细胞成熟率不同,分为5组:A组:30%≤卵母细胞成熟率60%;B组:60%≤卵母细胞成熟率70%;C组:70%≤卵母细胞成熟率80%;D组:80%≤卵母细胞成熟率90%;E组:卵母细胞成熟率≥90%。比较5组间的受精率、卵裂率、优质胚胎率、胚胎种植率、放弃周期率及临床妊娠率等。结果(1)各组基本资料比较,无显著性差异(P0.05);(2)受精率:A组(71.11%)显著低于C组(75.38%)、D组(77.40%)和E组(79.66%),且E组显著高于其他4组(P0.05);(3)卵裂率:A组(97.93%)显著低于D组(99.06%)和E组(99.13%)(P0.05);(4)周期取消率:A组(12.20%)显著高于C组(5.61%)、D组(3.57%)和E组(3.07%),B组(8.02%)显著高于D组和E组,C组显著高于E组(P均0.05);(5)胚胎种植率:A组(36.71%)显著低于D组(48.80%)(P0.05);(6)其余观察指标各组间比较均无显著性差异(P0.05)。结论卵母细胞成熟率较低时ICSI助孕的临床结局相对较差。卵母细胞成熟率低于70%时临床妊娠率开始下降,当卵母细胞成熟率低于60%时临床妊娠率进一步降低。     

非男性因素不孕高龄患者IVF和ICSI治疗的临床结局比较

目的 分析非男性因素不孕的高龄患者常规体外受精(IVF)和卵胞浆内单精子注射(ICSI)周期临床结局的差异。方法 回顾性分析2018年1月至2020年12月在本中心行IVF/ICSI治疗的非男性因素不孕高龄患者的临床资料,共纳入3 107个周期,包括2 876个IVF周期和231个ICSI周期。根据受精方式不同分为IVF组和ICSI组,比较两组患者的一般资料、受精和胚胎发育情况及临床妊娠结局。结果 一般情况比较：两组患者的平均年龄、不孕年限、体质量指数(BMI)、子宫内膜厚度均无显著性差异(P> 0.05);IVF组基础促卵泡生成素(FSH)水平显著高于ICSI组(P<0.05),IVF组抗苗勒管激素(AMH)水平、窦卵泡数(AFC)、获卵数均显著低于ICSI组(P<0.05)。受精和胚胎发育情况：IVF组正常受精率显著低于ICSI组(P<0.05),异常受精率显著高于ICSI组(P<0.05),两组患者的完全受精失败率、卵裂率、可移植胚胎率、优质胚胎率均无显著差异(P> 0.05)。临床妊娠结局情况：两组患者的临床妊娠率、流产率和活产率差异均无统计...     

不同来源冷冻复苏精子ICSI后对囊胚培养结局的影响

目的探讨不同来源冷冻复苏精子行卵胞浆内单精子注射(ICSI)后对囊胚培养结局的影响。方法回顾性分析2013年7月至2019年1月在本中心使用冷冻复苏精子共269个ICSI治疗周期的临床资料。根据冷冻复苏精子的来源,分为手淫组(94个周期)和手术组(175个周期),手术组根据手术位置不同又分为经皮附睾精子抽吸术(PESA组,82个周期)和睾丸精子抽吸术(TESA组,93个周期)两个亚组。比较分析不同来源冷冻复苏精子ICSI后胚胎的囊胚形成率、可利用囊胚率及优质囊胚率的差别。结果 (1)PESA组和TESA组比较,不孕年限和卵裂率差异有统计学意义(P<0.05),其它一般指标无统计学差异(P>0.05);组间囊胚形成率、可利用囊胚率、优质囊胚率均无显著性差异(P>0.05)。(2)手淫组和手术组的女方年龄、平均获卵数、MⅡ卵率、受精率、卵裂率差异均有统计学意义(P<0.05);组间囊胚形成率、可利用囊胚率、优质囊胚率差异均无统计学意义(P>0.05)。多重线性回归分析显示,手术组囊胚形成率显著低于手淫组(P<0.05),但组间可利用囊胚率及优质囊胚率均无...     

难治性高精子DNA碎片指数患者的睾丸精子在ICSI周期中应用的临床结局分析

目的 分析难治性高精子DNA碎片指数(DFI)患者的睾丸精子行卵胞浆内单精子注射(ICSI)的临床妊娠结局.方法 回顾分析2018年1月至2020年9月于我中心进行ICSI助孕的难治性高DFI患者临床资料,根据取精方式不同分为睾丸穿刺取精组(TESA组,n=27)和手淫射精组(EJ组,n=34),比较两组患者的基本资料...     

原发性不孕患者行早期补救ICSI和常规ICSI的临床应用分析

目的 探讨早期补救卵胞浆内单精子注射(r-ICSI)和常规卵胞浆内单精子注射(ICSI)对原发性不孕患者临床结局的影响。方法 回顾性分析2017年12月至2021年6月于山西医科大学第一医院生殖医学中心行常规ICSI治疗或因IVF全部/部分受精失败行早期r-ICSI治疗、年龄<35岁的原发性不孕患者的临床资料,共479个周期。根据授精方式的不同,分为r-ICSI组(行早期r-ICSI,共77个周期)和常规ICSI组(共402个周期);再根据患者的精液质量,将常规ICSI组分为精液参数正常的ICSI-1组(共144个周期)和精液参数异常的ICSI-2组(共258个周期)。比较3组患者的一般资料、实验室指标及妊娠结局。结果 3组患者间女方年龄、男方年龄、体质量指数(BMI)、不孕年限、基础FSH水平比较均无显著性差异(P>0.05)。r-ICSI组的MⅡ卵母细胞率、受精率、2PN率、可利用胚胎率、优质胚胎率显著高于ICSI-1组(P<0.05),而周期取消率显著低于ICSI-1组(P<0.001);r-ICSI组的MⅡ卵母细胞率、受精率、可利用胚胎率显著高于ICSI...     

不同来源精子对卵胞浆内单精子注射治疗结局的影响

目的分析不同来源精子对卵胞浆内单精子注射术(ICSI)治疗后的胚胎发育及治疗结局的影响。方法回顾性分析来我院行ICSI助孕治疗的144对不育夫妇(共154个周期),其中96个周期(A组,89对夫妇)的精子来源为严重少、弱精子症患者的射出精子,32个周期(B组,30对夫妇)为附睾精子,26个周期(C组,25对夫妇)为睾丸精子。比较三组经ICSI治疗后的2PN率、2PN卵裂率、优质胚胎率、种植率、妊娠率。结果 B组的2PN率、2PN卵裂率、优质胚胎率、妊娠率和种植率与A组相比,均无统计学差异(P0.05);C组2PN率、优质胚胎率低于A组、B组(P0.01),而妊娠率、种植率3组间无统计学差异(P0.05)。结论尽管睾丸精子行ICSI可能影响受精及早期胚胎发育,但与严重少弱精患者的射出精子及附睾来源的精子行ICSI的妊娠结局没有显著差异。     

Comparison of intracytoplastic sperm injection outcomes with fresh and frozen-thawed testicular sperm

Testicular sperm can be obtained from patients with non-obstructive azoospermia. However, there is not enough evidence concerning whether fresh or frozen-thawed sperm is better for successful pregnancy. We retrospectively compared initial treatment results of intracytoplasmic sperm injection using fresh and frozen-thawed testicular sperm in our institution. From August 1997 to May 2006, a total of 27 cases including 18 cases using fresh sperm (269 oocytes) and 9 cases using frozen sperm (97 oocytes) were evaluated. In the fresh and frozen sperm groups 33.4 and 32.9%, respectively, developed good quality embryo. There was no significant difference between the two groups in successful pregnancy and birth rates.     

采用冷冻精子与新鲜精子进行卵胞浆内单精子注射的结果比较

目的　比较冷冻精子与新鲜精子进行卵胞浆内单精子注射 ( ICSI)助孕技术的治疗效果。　方法　对 1 6 1对不育夫妇进行 1 6 3个辅助生殖技术治疗周期 ,其中采用冷冻精子 47个周期 ,比较了冷冻精子组与新鲜精子组 1 1 6个周期的受精率、卵裂率、A级胚胎率与临床妊娠率。　结果　冷冻精子组 (组 I)的受精率为 77.6 %、卵裂率为 92 .9%、A级胚胎率为 6 5.4%、临床妊娠率为 45.5% ;新鲜精液严重异常组 (组 II)分别为 54 .4%、94.0 %、45.7%及 2 5.0 % ;新鲜精液轻、中度异常组 (组 III)分别为 73 .5%、92 .5%、46 .8%及 2 9.3 %。组 I的受精率、A级胚胎率和临床妊娠率明显高于组 II( P<0 .0 5) ;与组 III比较无显著性差异 ( P>0 .0 5)。　结论　患者本身的精子质量直接影响 ICSI的受精率 ,精子冷冻复苏处理不影响 ICSI的受精率、卵裂率和优质胚胎率     

冻融胚胎与新鲜胚胎移植的新生儿结局比较

目的探讨冻融胚胎移植(FET)与新鲜胚胎移植的新生儿出生结局。方法收集2009年1月至2013年12月在我中心行体外受精-胚胎移植(IVF-ET)后单胎活产的3 961例患者的临床资料,根据移植胚胎的不同分为鲜胚移植组(2632例)和FET组(1 329例);随机选取同期在我院自然妊娠分娩单胎活产的1 500例产妇作为对照组。比较FET组和鲜胚移植组的助孕资料,以及3组新生儿的分娩孕周、出生体重、早产率、低出生体重比例、极低出生体重比例及巨大儿比例等。结果 FET组患者的平均年龄[(29.76±4.11)岁]显著低于鲜胚移植组[(30.60±4.22)岁](P0.05)。FET组和鲜胚移植组的新生儿出生体重[分别为(3 329.52±553.95)g和(3 247.89±508.00)g]均显著小于自然分娩组[(3 388.89±461.00)g],但FET组的新生儿出生体重显著高于鲜胚移植组(P均0.05)。FET组的早产率(8.50%)、低出生体重儿发生率(5.79%)显著低于鲜胚移植组(分别为10.22%、7.33%),而巨大儿出生率(11.44%)显著高于鲜胚移植组(6.50%)和自然分娩组(5.33%)(P均0.05)。FET组的早产发生率显著高于自然分娩组(3.60%)(P0.05)。校正年龄因素后,上述差异依然存在。结论 FET助孕后的新生儿结局有优于鲜胚移植周期的趋势,但仍劣于自然妊娠;FET周期巨大儿出生率的增加值得临床关注。     

Outcomes in couples undergoing ICSI: comparison between fresh and frozen-thawed surgically retrieved spermatozoa

We retrospectively evaluated the impact of cryopreservation on spermatozoa obtained from patients with azoospermia and used for intracytoplasmic sperm injection (ICSI). Frozen-thawed epididymal spermatozoa (FTEPS) was used in 34 couples, whereas frozen-thawed testicular spermatozoa (FTTS) was used in 50 couples for ICSI during assisted conception, and these results were compared with results using fresh spermatozoa for ICSI in the same individuals. The fertilization rate (FR) was significantly lower for FTTS (65.8%) but not for FTEPS (73.1%) compared with the FR using fresh spermatozoa (72.3% and 73.2% respectively). In contrast, neither the implantation nor the pregnancy rate was altered when FTEPS or FTTS was used. In conclusion, our results indicate that surgically retrieved spermatozoa can be efficiently used for ICSI after freezing and thawing without compromising the outcome.     

Comparison of conventional in vitro fertilisation and intracytoplasmic sperm injection outcomes in patients with moderate oligoasthenozoospermia

The method of choice for assisted reproductive technology treatment in vitro fertilisation (IVF) or intracytoplasmic sperm injection (ICSI) is usually based on the evaluation of male infertility factors. Decisions for couples with moderate oligoasthenozoospermia (OA) are often empirical because uniform treatment criteria are lacking. This study aimed to evaluate the effect of patients with moderate OA treated with conventional IVF and ICSI. A total of 199 couples with moderate OA undergoing their first IVF/ICSI cycle were included in the study. The patients were divided into two groups according to the type of insemination: conventional IVF group (n = 97) and ICSI group (n = 102). All patients were randomised to be inseminated either by conventional IVF or ICSI. The fertilisation rate, embryo quality, implantation rate and clinical pregnancy rate were examined. No differences in the fertilisation, implantation and pregnancy rates were observed between conventional IVF and ICSI groups (P > 0.05). However, the number of good‐quality embryos was significantly higher in the ICSI group than in the IVF group (P < 0.05). Couples with moderate OA did not influence on the overall clinical outcomes between IVF and ICSI treatments, and a negative influence by ICSI on blastocyst development was not confirmed.     

海拔3650米西藏地区体外受精新鲜周期和冻融周期胚胎移植后临床结局的分析

目的分析海拔3 650米的西藏地区行IVF/ICSI-ET助孕技术的临床妊娠结局。方法回顾性分析在西藏阜康妇女儿童医院生殖中心行IVF/ICSI-ET治疗的193例不孕症患者的303个治疗周期。根据女方患者年龄分为≤35岁组(n=238)和>35岁组(n=65),每个年龄段又根据移植胚胎类型分为新鲜周期组和冻融周期组,分析比较各组间的基本情况和妊娠结局。结果≤35岁组中的新鲜周期组与冻融周期组相比较,临床妊娠率(36.92%vs. 49.71%)、种植率(30.65%vs. 39.26%)、流产率(12.50%vs. 17.44%)、活产率(32.31%vs. 41.04%)、异位妊娠率(4.17%vs. 1.16%)以及多胎率(54.17%vs. 43.02%)均无显著差异(P>0.05);>35岁组中的新鲜周期组与冻融周期组相比较,临床妊娠率(23.08%vs. 40.38%)、种植率(16.00%vs. 27.17%)、流产率(33.33%vs. 19.05%)、活产率(15.38%vs. 23.08%)、异位妊娠率(0.00%vs. 0.00%)以及多胎率(3...     

不同BMI水平新鲜胚胎移植和全胚冷冻-首次冻融胚胎移植妊娠结局比较

目的比较不同体重指数(BMI)水平患者新鲜胚胎移植和全胚冷冻-首次冻融胚胎移植(FET)的妊娠结局。方法回顾性分析2009年1月至2018年5月于郑州大学第一附属医院生殖医学中心行首次新鲜胚胎移植(13 019个周期)和全胚冷冻后首次FET(3 546个周期)患者的临床资料。将研究对象按不同BMI分为正常体重组(18.5～23.9kg/m²)、超重组(23.9～27.9kg/m²)和肥胖组(≥28kg/m²)。分析不同BMI水平下鲜胚移植组和FET组患者的临床特征和妊娠结局,采用多元Logistic回归分析矫正影响妊娠结局的混杂因素;主要结局指标包括临床妊娠率、活产率、流产率。结果矫正各种混杂因素后,正常体重组中,FET组的临床妊娠率、活产率显著低于鲜胚移植组,而流产风险显著高于鲜胚移植组[P<0.05;临床妊娠率:aOR=0.80(95%CI:0.71-0.91);活产率:aOR=0.77(95%CI:0.68-0.86);流产率:aOR=1.28(95%CI:1.05-1.58)];超重组中,FET组的临床妊娠...     

Comparison of pregnancy and neonatal outcomes of intracytoplasmic sperm injection performed with frozen versus fresh testicular sperm

BackgroundIt remains controversial whether there is a difference in the prognosis of intracytoplasmic sperm injection (ICSI) using frozen or fresh testicular sperm in patients with obstructive azoospermia (OA). Moreover, in the available studies, few have tracked neonatal outcomes. This study aimed to compare the pregnancy and neonatal outcomes of ICSI using cryopreserved sperm versus fresh sperm collected by testicular sperm aspiration (TESA).MethodsA total of 317 OA patients treated with ICSI in a university affiliated hospital from January 2016 to December 2020 were included in this study. The participants were divided into two groups according to the type of sperm used for ICSI: frozen sperm group (n=154) and fresh sperm group (n=163). The pregnancy and neonatal outcomes of the two groups were compared.ResultsThe data produced by this study showed no significant statistical difference in the 2 pronuclei (2PN) fertilization rate, 2PN cleavage rate, high-quality blastocyst rate, and the average number of transferred embryos in the frozen and fresh sperm groups. Similarly, no difference was found in implantation rate, clinical pregnancy rate, multiple pregnancy rate, miscarriage rate, premature delivery rate, live birth rate, and gender ratio at birth (P>0.05). The average newborn birth weight was similar in both groups (2,932.61±728.40 vs. 3,100.32±515.64 g, respectively) (P>0.05). A higher incidence of low birthweight (LBW) newborns was found in the frozen sperm group (20.91% vs. 8.49%) (P<0.05). Multiple logistic regression analysis showed that LBW is related to single or twin pregnancies (P<0.01), but not sperm (frozen or fresh) (P>0.05). We further analyzed the twin and single pregnancies in the two groups separately, and found that the incidences of LBW were both similar (P>0.05). There was no difference in the Apgar scores at 1 min and 5 min after birth between the two groups (P>0.05).ConclusionsThe use of frozen testicular sperm by TESA was efficient for men with OA. There were similar pregnancy and neonatal outcomes following TESA-ICSI using frozen or fresh sperm in this retrospective study. Prospective investigations are needed for further validation.     

Clinical outcomes of microdissection testicular sperm extraction-intracytoplasmic sperm injection with fresh or cryopreserved sperm in patients with nonobstructive azoospermia

We performed this study to evaluate the clinical outcomes of microdissection testicular sperm extraction-intracytoplasmic sperm injection(micro-TESE-ICSI)treatment that used fresh or cryopreserved sperm in patients with nonobstructive azoospermia(NOA).A total of 338 NOA patients with 344 consecutive cycles received treatment in the reproductive medicine center of Peking University Third Hospital in Beijing,China,from January 2014 to December 2017.Fresh oocytes and fresh sperm were used in 222 patients with 234 cycles(Group A).Fresh oocytes and cryopreserved sperm were used in 116 patients with 110 cycles(Group B).We compared patient characteristics,embryonic development,and pregnancy outcomes between Groups A and B.There was no statistical difference in the patient characteristics,and no differences were observed with fertilization or quality embryo rates between Groups A and B.The rates of clinical pregnancy and live birth were both higher for Group A than those for Group B(both P<0.05).In conclusion,fresh testicular sperm appears to produce better ICSI outcomes than cryopreserved testicular sperm in patients with NOA.     

冻融复苏微量精子行卵细胞胞质内单精子注射术的疗效及临床妊娠结局分析

目的:回顾性分析123例无精子症患者经皮附睾精子抽吸术(PESA)或经皮睾丸精子抽吸术(TESA)后冻融复苏微量精子行卵细胞胞质内单精子注射术(ICSI)的疗效及临床妊娠结局情况。方法:将采用微量冻融PESA、TESA精子行ICSI的病例归为冻融精子组,采用新鲜PESA、TESA精子行ICSI的病例归为对照组。比较冻融精子组与新鲜精子组组间及组内的双原核(2PN)受精率、优质胚胎率、临床妊娠率、流产率、宫外孕率、多胎妊娠率有无统计学差异。结果:PESA精子冻融组与新鲜组受精率、优质胚胎率、临床妊娠率、流产率、宫外孕率及多胎妊娠率分别为75.67%vs76.49%,64.96%vs66.19%,55.21%vs57.22%,13.21%vs12.61%,3.77%vs5.41%,37.74%vs37.84%(P>0.05),TESA精子冻融组与新鲜组受精率、优质胚胎率、临床妊娠率、流产率、宫外孕率及多胎妊娠率分别为74.41%vs76.43%,64.63%vs66.35%,46.81%vs53.39%,18.18%vs14.55%,4.55%vs1.82%,37.74%vs37.84%,组间及组内均无统计学差异(P>0.05)。PESA精子与TESA精子冻融复苏成功率为70.07%vs62.67%,无统计学差异(P>0.05)。结论:微量PESA及TESA精子冻融技术对无精子症患者来说是一种安全、经济、有效的治疗方法;精子冷冻复苏技术有待于进一步提高;该技术是否会增加子代远期遗传风险仍有待于进一步探讨和研究。