血管紧张素Ⅱ诱导人胎肾系膜细胞凋亡及机制的初步研究

观察了血管紧张素Ⅱ的１型和２型受体及白细胞介素－１β转化酶在人胎肾系膜细胞表达２及其与肾小球系膜细胞凋亡的关系。采用６个月龄人胎肾培养肾小球系膜细胞,经１０＾＿８,１０＾－７,１０＾－６ｍｏｌ／Ｌ浓度的ＡｎｇⅡ诱导后,用逆转录－聚合酶链反应技术检测了胎肾系膜细胞的ＡＴ１,ＡＴ２受体和ＩＣＥ基因的ｍＲＮＡ表达情况。     

凝血酶调控肾小球系膜细胞产生明胶酶A、B的分子机制研究

在体外培养条件下观察了凝血酶对人肾小球系膜细胞表达细胞外基质降解酶－明胶酶Ａ及明胶酶Ｂ的调节作用。发现在无血清ＲＰＭＩ１６４０培养１２ｈ后,酶谱法显示系膜细胞培养上清液中ＭＭＰ－２活性极低,未检测出明显的ＭＭＰ－９酶解活性,但加入凝血酶后,可剂量依赖性地上调其ＭＭＰ－２,ＭＭＰ－Ｏ活性;ＲＴ－ＰＣＲ结果表明,在无血清ＲＰＭＩ１４６０培养１２ｈ后,系膜细胞仅表达低水平ＭＭＰ－９ｍＲＮＡ,加入凝血酶后     

Angiostatin K(1-3)基因的原核表达和活性鉴定

将入ＡｎｇｉｏｓｔａｔｉｎＫ（１－３）基因在原核细胞表达并鉴定其活性。构建和鉴定ＡｎｇｉｏｓｔａｔｉｎＫ（１－３）原核表达载体并在大脾性杆菌ＤＨ５α内表达,诱导表达蛋白用ＳＤＳ－ＰＡＧＥ分析和Ｗｅｓｔｅｒｎ ｂｌｏｔ鉴定,采用人脐带静脉内皮细胞抑制实验和鸡胚绒毛膜尿囊膜试验验证其活性。ＡｎｇｉｏｓｔａｔｉｎＫ（１－３）功能区基因在原核细胞获得成功表达,目的蛋白牛菌全总蛋白１５．７％。该表达蛋白捅抑     

耳石再生机制的实验研究

目的探讨耳石再生的机制。方法模拟飞行中气压的变化刺激豚鼠前庭耳石器,引起耳石的前体——球状物大量生成。其后１天和７天,电镜观察球状物和感觉上皮的变化,并用原位杂交技术观察感觉上皮各种细胞中ｃ－ｆｏｓｍＲＮＡ、ＢＭＰ１ｍＲＮＡ和ＢＭＰ２ｍＲＮＡ的表达情况。结果损伤后１天,电镜可见前庭椭圆囊斑表面布满球状物,支持细胞内充满囊泡,并见到支持细胞正在分泌球状物,ＢＭＰ２基因在囊斑的支持细胞及毛细胞中表达量增加,ｃ－ｆｏｓ基因在支持细胞、移形细胞及基底膜细胞中表达量明显增强。损伤后７天,ｃ－ｆｏｓ基因在囊斑中表达减弱,而ＢＭＰ１和ＢＭＰ２基因在囊斑各种细胞中的表达量均明显增加。结论支持细胞可分泌球状物。在耳石再生过程中,ｃ－ｆｏｓ、ＢＭＰ１和ＢＭＰ２基因在前庭囊斑感觉上皮中的表达量发生变化,提示它们参与了耳石再生。     

人β－干扰素在杆状病毒载体与Sf细胞体系中的表达

采用苜蓿尺蠖核多角休病毒（Ａｕｔｏｇｒａｐｈａｃａｌｉｆｏｒｎｉｃａｎｕｃｌｅａｒｐｏｌｙｈｅｄｒｏｓｉｓｖｉｒｕｓ，ＡｃＮＰＶ）ＤＮＡ中多角体蛋白基因启动子及其前后序列作为转移载体，将人β－干扰素（ＨｕＩＦＮ－β基因插入转移载体ｐＵＡｃ－５，构建成ｐＡｃ－ＩＦＮ－β载体。该质粒ＤＮＡ与野生型ＡｃＮＰＶＤＮＡ经ｌｉｐｏｆｅｃｔｉｎ共转染秋粘虫（Ｓｐｏｄｏｐｔｅｒａｆｒｕｇｉｐｅｒｄａ）传代细胞（Ｓｆ９细胞），利用重组病毒不产生多角体蛋白的特征，进行病毒空斑筛选。用核酸杂交方法鉴定出携带ＨｕＩＦＮ－β基因的主组病毒。用重组病毒感染Ｓｆ９细胞，可表达ｒＨｕＩＦＮ－β。在１．０×１０￣６细胞／ｍｌ感染上清中测定ｒＨｕＩＦＮ－β最高活性为３．０×１０￣６ＩＵ／ｍｌ，抗天然ＨｕＩＦＮ－β抗体能完全中和ｒＨｕＩＦＮ－β的抗病毒活性。     

应激对大鼠血浆内皮素与纤溶活性的影响及益气通络丹的保护作用

探讨应激时血浆内皮素（ＥＴ）与纤溶活性（ｔｉｓ－ｓｕｅ－ｔｙｐｅｐｌａｓｍｉｎｏｇｅｎａｃｔｉｖａｔｏｒ，ｔ－ＰＡ；ｐｌａｓｍｉｎｏｇｅｎａｃｔｉｖａｔｏｒｉｎｈｉｂｉｔｏｒ－１，ＰＡＩ－１）的变化，对于揭示应激性心血管疾病的发病机制及提供有效的临床...     

最新医学动态摘编

选择性杀癌细胞物：ＴＲＡＩＬ／Ａｐｏ－２Ｌ近期研究发现了一种能选择性诱导癌细胞发生自杀的蛋白ＴＲＡＩＬ／Ａｐｏ－２Ｌ（ＴＮＦ－ｒｅｌａｔｅｄａｐｏｐｌｔｏｓｉｓ－ｉｎｄｕｃｉｎｇ／Ａｐｏ－２ｌｉｇａｎｄ）。ＴＲＡＩＬ与肿瘤细胞表面高表达的ＤＲ４（ｄｅａｔｈｒｅｃｅｐｔｏｒ－４）、ＤＲ５结合而引起肿瘤细胞凋亡。而正常细胞表面高表达的ＤＣＲ１（Ｄｅｃｏｙｒｅｃｅｐｔｏｒ）也与ＴＲＡＩＬ结合但不引起细胞凋亡。ＴＲＡＩＬ能选择性地引起肿瘤细胞凋亡,但无严重的炎症反应及肝损害副作用,并且对肿瘤抑制基因Ｐ５…     

8-Br-cAMP和~(60)Co照射诱导宫颈癌Hela细胞凋亡及相关基因表达的研究

应用８ Ｂｒ ｃ Ａ Ｍ Ｐ 和６０ Ｃｏ 照射体外诱导 Ｈｅｌａ 细胞凋亡。探讨ｂｘ Ｌ ２ ,ｗ ｐ５３ ,ｍ ｐ５３ ,ｃ ｍ ｙｃｉ Ｎｏｓ 的基因表达和ｉ Ｎｏｓ , Ｆａｓ 蛋白表达之间的关系,为探讨无毒性８ Ｂｒ ｃ Ａ Ｍ Ｐ 对宫颈癌治疗的新途径提供依据。采用 Ｔ Ｕ Ｎ Ｅ Ｌ 法和 Ｆｅｕｌｇｅｎ 法进行细胞凋亡实验;采用本实验室创新的完整细胞原位 Ｒ Ｎ Ａ 斑点印迹技术检测 Ｆａｓ 和ｉ Ｎｏｓ蛋白,应用高速薄层色谱扫描仪对斑点印迹信号进行定量测定。结果８ Ｂｒ ｃ Ａ Ｍ Ｐ 和６０ Ｃｏ 照射均可上调 Ｈｅｌａ ｗｐ５３ ,ｉ Ｎｏｓ 的基因表达;下调ｍ ｐ５３ ,ｂｃｌ ２ ,ｃ ｍｙｃ 的基因表达。可增强ｉ Ｎｏｓ 蛋白质的酶活性,降低 Ｆａｓ 蛋白质的免疫反应性。显示出８ Ｂｒ ｃ Ａ Ｍ Ｐ 可能代替放疗作为一种治疗宫颈癌的新途径。     

尿激酶型纤溶酶原激活剂及其抑制剂PAI—1mRNAs的表达与人肝 …

对探讨尿激酶型纤溶酶原激活剂及其抑制剂ＰＡＩ－１ｍＲＮＡｓ的表达变化与人肝细胞癌浸润,转移间接关系,应用Ｎｏｒｈｅｒｎ印迹交技术对３０例新鲜活检肝组织作ｕＰＡ和ＰＡＩ－１ｍＲＮＡｓ表达的研究。     

白细胞介素12真核表达载体对DNA疫苗诱导免疫应答的影响

目的 观察白细胞介素１２真核表达载体（ｐＷＲＧ３１６９）对ＨＢＶ－ＳＤＮＡ疫苗（ｐＣＲ３．１－Ｓ）诱导Ｂａｌｂ／ｃ小鼠（Ｈ－２ｄ）的特异性细胞免疫应答及其对稳定表达ＨＢｓＡｇ的小鼠肥大细胞瘤Ｐ８１５细胞（Ｐ８１５－ＨＢＶ－Ｓ）成瘤性的影响。方法 肌肉注射ＤＮＡ疫苗主白细胞介素１２真核表达载体,背部皮下接种Ｐ８１５－ＨＢＶ－Ｓ细胞,观察成瘤情况,４ｈ＾５１Ｃｒ释放法检测小鼠脾细胞ＣＴＬ活性。结果：对     

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version

The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.     

Endovascular management of carotid-cavernous fistulas

Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas （CCF） and their complications such as pseudoaneurysms. Methods： Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% （4/22）. A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% （8/22）. Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents.     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

Do Balance Board Training Programs Reduce the Risk of Ankle Sprains in Athletes?

Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).     

High Intensity Interval Training:New Insights

KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief，intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.，≥90%of VO2 peak).     

Developmental validation of the PowerPlex(®) ESI 16 and PowerPlex(®) ESI 17 Systems: STR multiplexes for the new European standard

In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega^® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex^® ESI 16 (European Standard Investigator 16) and the PowerPlex^® ESI 17 Systems. The PowerPlex^® ESI 16 System combines the 11 loci compatible with the UK National DNA Database^®, contained within the AmpFlSTR^® SGM Plus^® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR^® SGM Plus^® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex^® ESI 17 System amplifies the same loci as the PowerPlex^® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR^® SGM Plus^® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex^® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors.