早期负压封闭引流治疗深Ⅱ度烧伤创面的临床疗效评价

Objective To evaluate the clinical curative effect of applying vaccum sealing drainage (VSD) therapy in treating deep partial-thickness burn wound at the initial stage prospectively, and to provide the basis for its clinical application. Methods Twenty-two patients with about 10% TBSA burn of the lower limbs, and in which partial-thickness wound exceeded 1% TBSA in each limb, were admitted to our hospital within 3 hours after burn from May 2009 to March 2010. Wounds in each patient were divided into VSD treatment group (treated with VSD therapy) and control group (treated with 10 g/L silver sulfadia-zine cream) based on the principles of symmetry of location, identical deepness, and similarity in size etc. The amount of water evaporation, the swelling intensity, the status of bacterial colonization, the degree of pain, the healing time, and the quality of healing of wounds in 2 groups were observed and compared. Data were processed with t test and rank-sum test. Results The observation was completed in 21 patients. All of the wounds were treated within 4 hours post burn (PBH). The amount of water evaporation of the normal skin and burn wounds before dressing coverage in VSD treatment group was respectively close to that in control group (with t value respectively 1.310, - 0. 911, P values all above 0.05) ; the amount of water evaporation on the surface of dressing in VSD treatment group [(44. 3 ±3.9) mL·h-1·m-2] was less than that in control group [(66.1 ±6.4) mL · h-1· m-2, t = -11.39, P <0.01]. In VSD treatment group, the circumference of proximal thigh increased (3.48 ±0.35) and (2.51 ±0.21) cm on post burn day (PBD) 3 , 7 as compared with that on PBH 5 , which was respectively smaller than that [(8.02 ± 0.41) , (3. 99 ± 0. 32) cm] in control group (with t value respectively 4. 110, 3. 569, P values all below 0. 01). Positive bacteria' culture rate on PBD 10 of each group was respectively lower than that at admission (with Z value respectively -3.220, -3.870, P values all below 0. 01) , and there was no significant statistical difference between 2 groups at admission or on PBD 10 (with Z value respectively - 0. 894, 0.000, P values all above 0.05). The wound surface in VSD treatment group was weak acidic (pH value 7. 12 ±0.06) on PBD 10,and it was neutral (pH value 7.41 ±0. 13) in control group. The wound pain degree in control group on PBD 1,3,7 was respectively higher than that in VSD treatment group (with t value respectively - 16. 132, -21.230, -16.453, P values all below 0.01). There was no significant statistical difference between 2 groups in healing time of wounds (t =1. 186, P >0.05). The healing quality of wounds in VSD treatment group (100. 00% , 100. 00%) 2 or 3 months after burn was better than that in control group (19. 05% , 85. 71%) (with Z value respectively -11.638, -3. 870, P values all below 0.01). Conclusions Early application of VSD therapy cannot expedite the healing process of deep partial-thickness burn wounds, but it can improve the healing quality. It is one of the effective methods to deal with deep partial-thickness burn wounds, which is worthy of clinical attention and further research.     

早期负压封闭引流治疗深Ⅱ度烧伤创面的临床疗效评价

Objective To evaluate the clinical curative effect of applying vaccum sealing drainage (VSD) therapy in treating deep partial-thickness burn wound at the initial stage prospectively, and to provide the basis for its clinical application. Methods Twenty-two patients with about 10% TBSA burn of the lower limbs, and in which partial-thickness wound exceeded 1% TBSA in each limb, were admitted to our hospital within 3 hours after burn from May 2009 to March 2010. Wounds in each patient were divided into VSD treatment group (treated with VSD therapy) and control group (treated with 10 g/L silver sulfadia-zine cream) based on the principles of symmetry of location, identical deepness, and similarity in size etc. The amount of water evaporation, the swelling intensity, the status of bacterial colonization, the degree of pain, the healing time, and the quality of healing of wounds in 2 groups were observed and compared. Data were processed with t test and rank-sum test. Results The observation was completed in 21 patients. All of the wounds were treated within 4 hours post burn (PBH). The amount of water evaporation of the normal skin and burn wounds before dressing coverage in VSD treatment group was respectively close to that in control group (with t value respectively 1.310, - 0. 911, P values all above 0.05) ; the amount of water evaporation on the surface of dressing in VSD treatment group [(44. 3 ±3.9) mL·h-1·m-2] was less than that in control group [(66.1 ±6.4) mL · h-1· m-2, t = -11.39, P <0.01]. In VSD treatment group, the circumference of proximal thigh increased (3.48 ±0.35) and (2.51 ±0.21) cm on post burn day (PBD) 3 , 7 as compared with that on PBH 5 , which was respectively smaller than that [(8.02 ± 0.41) , (3. 99 ± 0. 32) cm] in control group (with t value respectively 4. 110, 3. 569, P values all below 0. 01). Positive bacteria' culture rate on PBD 10 of each group was respectively lower than that at admission (with Z value respectively -3.220, -3.870, P values all below 0. 01) , and there was no significant statistical difference between 2 groups at admission or on PBD 10 (with Z value respectively - 0. 894, 0.000, P values all above 0.05). The wound surface in VSD treatment group was weak acidic (pH value 7. 12 ±0.06) on PBD 10,and it was neutral (pH value 7.41 ±0. 13) in control group. The wound pain degree in control group on PBD 1,3,7 was respectively higher than that in VSD treatment group (with t value respectively - 16. 132, -21.230, -16.453, P values all below 0.01). There was no significant statistical difference between 2 groups in healing time of wounds (t =1. 186, P >0.05). The healing quality of wounds in VSD treatment group (100. 00% , 100. 00%) 2 or 3 months after burn was better than that in control group (19. 05% , 85. 71%) (with Z value respectively -11.638, -3. 870, P values all below 0.01). Conclusions Early application of VSD therapy cannot expedite the healing process of deep partial-thickness burn wounds, but it can improve the healing quality. It is one of the effective methods to deal with deep partial-thickness burn wounds, which is worthy of clinical attention and further research.     

The change of angiotensin Ⅱ production and its receptor expression during wound healing: possible role of angiotensin Ⅱ in wound healing

Objective This study was undertaken to observe the change in the local level of angiotensin Ⅱ (Ang Ⅱ) and the expression of its corresponding receptors AT1 and AT2 during wound healing, and explore the possible role of Ang Ⅱ in wound healing . Methods A model of full-thickness cutaneous wound was developed on the back of C57/BL6 mice. Specimens were taken from the wound of each mouse on the day 0, 1, 3, 5, 7, 9, 11, 13 and 15 after wounding. The change in the generation of Ang Ⅱ in wounded tissue during the healing process was detected with ELISA. The proliferation and the apoptosis of cells were detected by bromodeoxyuridine (Brdu) and terminal deoxyuncleotidyl transferase mediated deoxyuridine triphosphate nick end labeling (TUNEL) method in wounded skin during the healing process, respectively. The cellular localization and the mRNA level change of Ang Ⅱ receptors in wounded tissue during healing were detected with immunostaining and RT-PCR. Results Ang Ⅱ produced in wounded skin was increased in the first 7 days to reach the peak, and then gradually decreased during wound healing. BrdU labeling index was increased gradually in the first 7 days to reach the peak, and then gradually decreased during wound healing. The number of TUNEL-positive cells was increased slowly in the first 7 days after wounding. The increase in the number of TUNEL-positive cells was more markedly after epithelization of the wound. In normal mice, AT1 and AT2 receptor were found positively expressed in the whole epidermal layer, while positive expression was only found in the endothelial cells of the capillary vessels within the dermal layer, and positive expression was also found in appendages of the skin, i.e. hair follicle, sweat gland and sebaceous gland respectively. Positive staining signal of both AT1 and AT2 receptors were increased in the first 7 days to reach the peak, then gradually decreased. Expression of AT2R was increased again following the epithelization of wound. The result of RT-PCR showed that the expression of both AT1 and AT2 receptors was detectable, and AT1 receptor was increased in the first 7 days to the peak, and then gradually decreased during wound healing, while AT2 receptor expression reached its peak value on day 7, then gradually decreased, and increased again following the epithelization of wound. Conclusions These results indicate that Ang Ⅱ participate in wound repair and related to remolding in the late stage of wound healing through the change in production of angiotensin Ⅱ and expression of AT1 and AT2 receptors. AT1 receptor might be closely associated with cell proliferation,while AT2 receptor might play a role in cell apoptosis and remolding during wound healing.     

The change of angiotensin Ⅱ production and its receptor expression during wound healing: possible role of angiotensin Ⅱ in wound healing

Objective This study was undertaken to observe the change in the local level of angiotensin Ⅱ (Ang Ⅱ) and the expression of its corresponding receptors AT1 and AT2 during wound healing, and explore the possible role of Ang Ⅱ in wound healing . Methods A model of full-thickness cutaneous wound was developed on the back of C57/BL6 mice. Specimens were taken from the wound of each mouse on the day 0, 1, 3, 5, 7, 9, 11, 13 and 15 after wounding. The change in the generation of Ang Ⅱ in wounded tissue during the healing process was detected with ELISA. The proliferation and the apoptosis of cells were detected by bromodeoxyuridine (Brdu) and terminal deoxyuncleotidyl transferase mediated deoxyuridine triphosphate nick end labeling (TUNEL) method in wounded skin during the healing process, respectively. The cellular localization and the mRNA level change of Ang Ⅱ receptors in wounded tissue during healing were detected with immunostaining and RT-PCR. Results Ang Ⅱ produced in wounded skin was increased in the first 7 days to reach the peak, and then gradually decreased during wound healing. BrdU labeling index was increased gradually in the first 7 days to reach the peak, and then gradually decreased during wound healing. The number of TUNEL-positive cells was increased slowly in the first 7 days after wounding. The increase in the number of TUNEL-positive cells was more markedly after epithelization of the wound. In normal mice, AT1 and AT2 receptor were found positively expressed in the whole epidermal layer, while positive expression was only found in the endothelial cells of the capillary vessels within the dermal layer, and positive expression was also found in appendages of the skin, i.e. hair follicle, sweat gland and sebaceous gland respectively. Positive staining signal of both AT1 and AT2 receptors were increased in the first 7 days to reach the peak, then gradually decreased. Expression of AT2R was increased again following the epithelization of wound. The result of RT-PCR showed that the expression of both AT1 and AT2 receptors was detectable, and AT1 receptor was increased in the first 7 days to the peak, and then gradually decreased during wound healing, while AT2 receptor expression reached its peak value on day 7, then gradually decreased, and increased again following the epithelization of wound. Conclusions These results indicate that Ang Ⅱ participate in wound repair and related to remolding in the late stage of wound healing through the change in production of angiotensin Ⅱ and expression of AT1 and AT2 receptors. AT1 receptor might be closely associated with cell proliferation,while AT2 receptor might play a role in cell apoptosis and remolding during wound healing.     

Comparison of minimally invasive reduction through a bone tunnel combined with Jail screwing and posterolateral locking plating for simple posterolateral tibial plateau fractures北大核心CSCD

Objective To compare the clinical effects of minimally invasive reduction through a bone tunnel combined with Jail screwing and those of posterolateral locking plating in the treatment of simple posterolateral tibial plateau fractures. Methods A retrospective analysis was conducted of the data of 48 patients who had been operatively treated and completely followed up at Department of Orthopedics, Suqian Hospital of Nanjing Drum Tower Hospital Group for simple posterolateral tibial plateau fractures from October 2016 to October 2020. There were 26 males and 22 females, aged from 35 to 68 years. They were divided into a minimally invasive group (25 cases subjected to minimally invasive reduction through a bone tunnel combined with Jail screwing) and an incision group (23 cases subjected to posterolateral locking plating) according to their surgical methods. The operation time, incision length, intraoperative blood loss, fracture healing time, cumulative fluoroscopy time, hospital stay and posterior inclination angles of the tibial plateau and Hospital for Special Surgery (HSS) knee function scores at 1, 3, 6, 9, and 12 months after operation were compared between the 2 groups. Complications in the 2 groups of patients were recorded. Results There was no significant difference in the preoperative general data between the 2 groups, showing comparability (P>0.05). The 48 patients were followed up for 12 to 36 months (average 16.5 months). The minimally invasive group was significantly better than the incision group in operation time [(42.6±9.1) min versus (65.7±11.5) min], incision length [(4.0±0.4) cm versus (15.0±1.5) cm], intraoperative blood loss[(22.6±5.8) mL versus (31.5±8.8) mL], hospital stay [(7.6±1.4) d versus (11.1±2.4) d], and HSS score one month after operation [(84.8±1.9) points versus (72.9±4.1) points], but the cumulative fluoroscopy time in the incision group [(4.1±1.4) s]was significantly less than that in the minimally invasive group [(22.3±4.2) s] (P<0.05). There were no significant differences in fracture healing time, HSS scores at 3, 6, 9, or 12 months after operation, or posterior inclination angle of the tibial plateau between the 2 groups (P>0.05). There were no such complications as wound infection, vascular injury, internal fixation failure, nonunion or malunion of fractures in either of the 2 groups. Two cases in the incision group presented with symptoms of common peroneal nerve injury but recovered 3 months after operation. Conclusions Although both minimally invasive reduction through a bone tunnel combined with Jail screwing and posterolateral locking plating can achieve satisfactory outcomes in the treatment of simple posterolateral tibial plateau fractures, the minimally invasive technique is preferable because it shows the advantages of a smaller incision, less bleeding, shorter operation time, a lower operation risk, quicker postoperative recovery and shorter hospital stay. © 2022 Chinese Journal of Orthopaedic Trauma. All rights reserved.     

Treatment of tibiofibular fractures of Gustilo type Ⅲ by vacuum, sealing drainage and external fixation

Objective To report clinical efficacy of vacuum sealing drainage(VSD)and external fixation in the treatment of tibiofibular fractures of Gustilo type Ⅲ. Methods From October 2006 to November 2009,19 cases of Gustilo type Ⅲ tibiofibular fracture were treated in our department.They were 14men and 5 women,with an average age of 30.1 years(from 17 to 62 years).There were 9 cases of type ⅢA,8 cases of Type ⅢB 2 cases of typeⅢC.After emergency debridement,all the fractures were fixed with an extemal fixator following indirect reduction.The wounds were sealed with VSD.When the wounds were clean and granulation appeared,they were repaired by direct suture(5 cases),split-thickness skin graft(9 cases)and flap transposition(5 cases). Results The 19 cases were followed up for 12 to 24 months,with an average of 16.3 months.A11 the wounds were closed'after VSD for 5 to 17 days.Wound infection occurred in 2 cases and was finally repaired by flap transposition following repeated debridement and VSD.The fractures healed after 4 to 10 months(average,5.6 months). Conclusion External fixation combined with VSD is a simple and effective treatment for tibiofibular fractures of Gustilo type Ⅲ.because it can provide not only rapid fixation but also early wound sealing to facilitate fracture healing and reduce incidence of complications.     

荷负电气溶胶治疗对大鼠烫伤创面白细胞介素-8、10表达的影响及意义

目的 观察荷负电气溶胶治疗对大鼠烫伤创面愈合过程中白细胞介素(IL)-8、IL-10表达的影响,探讨荷负电气溶胶治疗促进创面愈合的作用机制.方法 制作SD大鼠深Ⅱ度烫伤模型,采用分组对照方法,将40只鼠随机分为治疗组(n1=20)和对照组(n2=20).治疗组应用荷负电气溶胶治疗,每次1.5 h,每天2次,直至创面愈合;对照组不作荷负电气溶胶治疗.伤后第1～11天分别取创面标本制作切片,采用免疫组织化学和图像分析方法,检测创面愈合过程中IL-8和IL-10表达水平.结果 创面平均愈合时间治疗组为(7.00±1.15)d,对照组为(9.00±1.34)d,治疗组创面愈合时间明显提前(P＜0.01).免疫组织化学显示,两组IL-8均在伤后第1天开始表达.主要位于多核粒细胞和单核细胞;第3天表达明显增多达高峰,并见大量成纤维细胞表达,治疗组的峰值明显低于对照组,差异有统计学意义(P＜0.01),第5～11天表达水平迅速下降.两组IL-10伤后第1天在淋巴细胞和单核细胞均有表达;第3天开始有角质细胞表达并达高峰,第5～11天表达水平缓慢下降,但治疗组要明显高于对照组,第3～11天差异有统计学意义(P＜0.01).结论 荷负电气溶胶治疗能有效抑制创面IL-8的表达及促进IL-10的表达,缩短炎症进程,从而加速创面愈合.

Abstract：

Objective To discuss the influence of aerosol bioelectricity on the expression of interleukin (IL) -8 and IL-10 in wound healing of burned rats. Methods The deep Ⅱ degree scalding models were established in Sprague Dawley (SD) rats. Rats were randomly divided into experimental group (n1 =20) and control group (n2 =20). The rats in experimental group were treated with aerosol bioelectricity.Samples were collected at the first to eleventh day post-scalding. Immunohistochemistry and image analysis methods were conducted to examine the expression of IL-8 and IL-10 in both experimental and control groups. Results The average wound healing time in experimental group was 7. 00 ± 1. 15 days, and that in control group was 9. 00 ± 1. 34 days. IL-8 and IL-10 were observed mainly in polylmorphonuclear and mononuclear cells in both experimental and control groups on the 1 st day. On the third day, fibroblasts abounded, IL-8 expression was increased evidently and reached a peak. The peak value (6. 73 ± 1. 36) in experimental group was lower significantly than that in control group ( 2. 85 ± 0. 72, P ＜ 0. 01). From the 5th to 11th day, IL-8 expression was declined rapidly. IL-10 was expressed in keratode cells and had the peak value in experimental group (1. 24 ±0. 15) and control group (5. 69 ± 1. 32) on the 3rd day. IL-10 expression was declined gradually from the 5th to 11th days. The expression level of IL-10 in experimental group was significantly higher than in control group from the 3rd day to 11th days post-scalding (P＜0. 01). On the 3rd day, both IL-8 and IL-10 in experimental and control groups were expressed abundantly , and there was negative relationship between them (r = - 0. 862, P ＜ 0. 01). Conclusion Aerosol bioelectricity can indicate active cells proliferation through down-regulating the expression of IL-8 and up-regulating the expression of IL-10, accelerating burned wound healing.     

外固定支架结合负压封闭引流技术治疗Gustilo Ⅲ型胫腓骨开放性骨折

目的 探讨外固定支架结合负压封闭引流(VSD)技术治疗Gustilo Ⅲ型胫腓骨开放性骨折的疗效.方法 2006年10月至2009年11月采用外崮定支架结合VSD技术治疗19例Gustilo Ⅲ型胫腓骨开放性骨折患者,男14例,女5例;年龄17～62岁,平均30.1岁.开放性损伤按Gustilo分型:ⅢA型9例,ⅢB型8例,ⅢC型2例.所有患者均行急诊清创、骨折复位后外固定支架固定,采用VSD技术关闭创面,待创面洁净、肉芽生长后通过直接缝合(5例)、游离植皮(9例)或转移皮瓣(5例)等方法修复软组织创面.结果 19例患者术后获12～24个月(平均16.3个月)随访.所有患者经VSD治疗5～17 d(平均9.3 d)后关闭创面.2例因创面感染、组织坏死渗出,经多7欠清创并更换VSD敷料后,创面最终通过转移皮瓣得以修复.骨折愈合时间为4～10个月(平均5.6个月).结论外固定支架结合VSD技术治疗Gustilo Ⅲ型胫腓骨开放性骨折能在迅速有效地稳定骨折的同时,早期闭合创面,促进骨折愈合,减少并发症的发生.

Abstract：

Objective To report clinical efficacy of vacuum sealing drainage(VSD)and external fixation in the treatment of tibiofibular fractures of Gustilo type Ⅲ. Methods From October 2006 to November 2009,19 cases of Gustilo type Ⅲ tibiofibular fracture were treated in our department.They were 14men and 5 women,with an average age of 30.1 years(from 17 to 62 years).There were 9 cases of type ⅢA,8 cases of Type ⅢB 2 cases of typeⅢC.After emergency debridement,all the fractures were fixed with an extemal fixator following indirect reduction.The wounds were sealed with VSD.When the wounds were clean and granulation appeared,they were repaired by direct suture(5 cases),split-thickness skin graft(9 cases)and flap transposition(5 cases). Results The 19 cases were followed up for 12 to 24 months,with an average of 16.3 months.A11 the wounds were closed'after VSD for 5 to 17 days.Wound infection occurred in 2 cases and was finally repaired by flap transposition following repeated debridement and VSD.The fractures healed after 4 to 10 months(average,5.6 months). Conclusion External fixation combined with VSD is a simple and effective treatment for tibiofibular fractures of Gustilo type Ⅲ.because it can provide not only rapid fixation but also early wound sealing to facilitate fracture healing and reduce incidence of complications.     

Animal study on expression of laminin and fibronectin in cornea during wound healing following alkali burn

Objective:To observe the expression of laminin and fibronectin in alkali-burned corneas in rats.Methods:A total of 18 normal Wistar rats were randomly divided into 6 groups(n=3 in each group).For each rat, one eye was injured by alkali burn,the other one was taken as the normal control.Then all the corneas were surgically removed and the expression of laminin and fibronectin was observed with immunohistochemistry respectively at 7 hours,1 day,3 days, 7 days,14 days and 28 days after alkali burn.Results:Compared with that of the normal controls, the expression of laminin and fibronectin of the burned eyes was dramatically higher at 7 hours, reached peak at 14 days and decreased to the normal level at 28 days after alkali burn.Conclusions:In the process of wound healing after alkali burn, the expression of laminin and fibronectin increases dramatically, which suggests that laminin and fibronectin may participate in the process of corneal wound healing.     

中国商环包皮环切术与传统包皮环切术的临床对比研究

目的 对比分析中国商环包皮环切术和传统包皮环切术的疗效和手术并发症.方法 有完整随访资料的479例中国商环包皮环切术患者和354例传统包皮环切术患者,两组患者年龄、包皮情况比较差异无统计学意义(P＞0.05).对比分析其手术时间、疼痛评分、失血量、术后并发症、术后外观满意情况、伤口愈合时间、治疗费用等.结果 商环组和传统手术组手术时间分别为(5±1)、(27±5)min;出血量分别为(0.98±1.14)、(8.30±3.60)ml;手术时疼痛评分分别为0.25±0.54、3.29±1.57;术后24 h疼痛评分分别为1.63±0.87、5.56±1.42;术后并发症发生率分别为6.89%(33/479)、13.28%(47/354);创口愈合时间分别为(20±5)、(13±2)d;外观满意率分别为99.79%(478/479)、92.37%(327/354);治疗费用分别为(871±52)、(554±46)元.与传统手术组相比,商环组手术时间短,失血量少,患者痛苦小,外观满意率高,并发症发生率低(P＜0.05).但商环组创口愈合时间较长,治疗费用较高(P＜0.05).结论 用中国商环行包皮环切术是对传统包皮环切术的简化和改进,具有手术时间短、术中出血量少、疼痛轻、术后外观满意度高、患者易于接受等优点.

Abstract：

Objective To compare the efficacy and complications of Chinese Shang Ring circumcision with conventional circumcisiom Methods Clinical data of 479 cases of Chinese Shang Ring circumcision and 354 cases of conventional circumcision with complete follow-up were analyzed.Comparisons were made between the two groups on operation time,pain score,blood loss,postoperative complications,postoperative satisfaction with penile appearance,wound healing time and treatment costs. Results There was no statistical difference in age and foreskin status between the two groups (P＞0.05).For the Shang Ring group,the operation time was(5±1)rain,blood loss was (0.98±1.14)ml,pain score during operation was 0.25±0.54,24-hour pain score after operation was 1.63±0.87,the postoperative complication rate was 6.89% (33/479),wound healing time was (20±5)d,the satisfaction rate of appearance was 99.79% (478/479),and treatment cost was (871±52) yuan.For the conventional group,the operation time was (27±5) min,blood loss was (8.30±3.60)ml,pain score during operation was 3.29±1.57,24-hour pain score after operation was 5.56±1.42,the postoperative complication rate was 13.28%(47/354),wound healing time was (13±2)d,satisfaction rate of appearance was 92.37% (327/354),and treatment cost was (554±46) yuan.Compared with the conventional group,the Shang Ring group had a shorter operation time,less blood loss,less pain score,higher appearance satisfaction rate and a lower complication rate (P＜0.05).But wound healing time was longer and treatment cost was higher in the Shang Ring group (P＜0.05). Conclusions Chinese Shang Ring circumcision is simpler and an improved approach over conventional circumeision with shorter operative time,less blood loss,less pain,relatively lower complication rate and higher satisfaction and acceptability.     

负压封闭引流治疗四肢开放损伤创面

目的探讨负压封闭引流(VSD)治疗四肢开放损伤创面的价值。方法将236例四肢开放损伤创面患者按治疗方法分为两组:VSD组126例,创面面积89.0~205.0(142.57±27.46)cm~2;常规换药组110例,创面面积93.0~202.0(141.94±27.98)cm~2。观察两种治疗方法的疗效。结果创面愈合时间、住院时间、感染情况VSD组均优于常规换药组,差异均有统计学意义(P0.05)。结论应用VSD治疗四肢开放损伤创面较常规换药治疗的愈合时间缩短、住院时间缩短,感染发生率低。     

负压封闭引流促进糖尿病足溃疡的愈合

目的分析负压封闭引流(vacuum sealing drainage,VSD)能否促进糖尿病足溃疡的愈合。方法回顾分析自2015年1月至2019年12月,北部战区总医院烧伤整形科收治的糖尿病足溃疡患者60例,并根据患者的治疗方式分为常规治疗组(30例)和VSD治疗组(30例)。统计对比分析两组患者的平均换药次数、平均愈合时间、疼痛程度及满意度。搜集治疗前和治疗14 d后的肉芽组织进行HE染色和VEGF免疫组化染色,分析创面愈合情况以及VEGF的表达情况。结果VSD治疗组换药次数[(5.40±0.28)次]显著少于常规治疗组[(31.41±1.11)次],组间比较P<0.05;VSD治疗组平均愈合时间(29.38±0.63)d显著短于常规治疗组(50.81±2.15)d,组间比较P<0.05;VSD治疗组患者的痛苦程度明显轻于常规治疗组,满意度显著优于常规治疗组,组间比较P<0.05。结论VSD治疗能够促进创面成纤维细胞的增殖、减少炎性细胞的浸润、促进VEGF的表达及创面的愈合。     

负压封闭引流术治疗胸腰椎病变术后切口感染的临床疗效

目的观察负压封闭引流(VSD)治疗胸腰椎病变术后切口感染的临床疗效。方法对54例胸腰椎病变术后切口感染患者分别采用置管冲洗引流(对照组,23例)和VSD(VSD组,31例)治疗。比较两组抗生素应用时间、创口局部治疗时间、引流管阻塞情况,创口愈合情况,观察VSD副反应,评估VSD的疗效。结果54例患者均获随访,时间6~15个月。抗生素应用时间:对照组(20.1±5.6)d,VSD组(14.1±2.1)d;创口局部治疗时间:对照组(25.2±6.2)d,VSD组(15.0±4.6)d;引流管阻塞次数:对照组13次(13/33),VSD组11次(11/81);创口愈合情况:对照组15例(15/23),VSD组29例(29/31)。两组上述指标比较差异均有统计学意义(P0.05)。VSD组出现皮肤发红、发痒5例,皮疹3例。结论 VSD治疗胸腰椎病变术后切口感染能缩短感染伤口治疗时间,促进创口愈合。     

早期电动磨痂结合不同种敷料覆盖修复小儿深Ⅱ度烧伤创面的疗效观察

目的：观察小儿深Ⅱ度烧伤创面早期电动磨痂后结合不同种敷料覆盖的治疗效果.方法：将2010年1月-2012年1月收治的60例四肢和躯干热液烫伤患儿随机分为凡士林油纱组、生物敷料组和负压封闭引流（VSD）组,每组20例.各组患儿在磨痂后,根据分组的不同,分别应用凡士林油纱、异种脱细胞真皮基质或VSD覆盖创面.术后观察创面愈合时间、愈合质量、换药次数、治疗费用.结果：生物敷料组、VSD组的创面愈合时间分别为（16.3±1.9）d和（16.9±1.8）d,明显短于凡士林纱布组[（19.2±2.5）d,P＜0.05];生物敷料组、VSD组反映瘢痕增生程度的温哥华评分分别为（4.5±0.7）分和（4.1±0.8）分,明显低于凡士林油纱组[（8.6±1.2）分,P＜0.01];VSD组的换药次数[（3.0±0.5）次]明显低于凡士林油纱组[（6.9±0.7）次,P＜0.05]和生物敷料组[（7.2±0.8）次,P＜0.01];凡士林油纱组的住院费用[（6 560.34±1 230.00）元],明显低于生物敷料组[（12 028.24±1 380.00）元,P＜0.01]和VSD组[（14 125.40±1 560.00）元,P＜0.01].结论：早期磨痂应用异种生物敷料和VSD覆盖较普通油纱覆盖能明显促进小儿深Ⅱ度烧伤创面愈合,缩短住院时间,显著提高创面愈合质量,减少换药次数,减轻患儿痛苦,但住院费用较高,临床上应根据患儿具体情况,灵活选择磨痂后创面覆盖物.     

心脏不停跳修补室缺后肌钙蛋白T分泌变化

目的 观察心脏不停跳修补室缺后肌钙蛋白T(cTnT)分泌变化.方法 共施行单纯室间隔缺损(VSD)合并轻、中度肺动脉高压患者202例.VSD(0.7 ±0.2)cm;膜部185例,干下型15例,肌部2例.心胸比例0.49 ±0.18.随机分为3组:A组(晶体组)86例;B组(血灌组)51例;C组(不停跳组)65例.术中切取右心耳行电镜观察.术后24 h和72 h抽血监测cTnT.结果 无气栓、死亡、严重低心排、肾功能或呼吸功能衰竭等严重并发症发生.术后1～2 d轻度血红蛋白尿5例.术后1～3 d暂时性房室分离7例.直径＜2 mm残余漏2例.C组2例术中室颤.手术后24 h的cTnT:A组(0.75±0.25)μg/L与B组(0.69 ±0.20)μg/L,P＞0.05,C组(0.55 ±0.18)μg/L与A、B组差异有统计学意义(P＜0.01).术后72 h的cTnT:A组(0.53 ±0.19)μg/L与C组(0.55±0.13)μg/L,P＞0.05,A组与B组(0.43±0.13)μg/L,P＜0.01.电镜发现3组之间,以C组损害略轻,A组相对较重.结论 心脏跳动下修补可明显减少手术时间,由此可以减少由手术对全身造成的创伤,并且方法安全、可行.心脏不停跳修补的cTnT分泌变化反映了心肌保护效果:并不优于血灌组,两者皆优于晶体停跳组.

Abstract：

Objective To estimate the myocardial protective effect of the operation.Methods From January 2004 to June 2009,we implemented ventricular septal defect (VSD) repairs in 202 patients with simple VSD and mild and moderate pulmonary hypertension,including 98 males and 104 females.Theaverage size of VSD was (0.7 ±0.2) cm.There were 185 patients with perimenbranous VSD,15 patients with lower trunk VSD,and 2 patients with muscular VSD.The average cardiothoracic ratio (CTR) was 0.49 ±0.18.202 patients were randomly divided into 3 groups:group A (crystalloid group) with 86 cases;group B (hemoperfusion group) with 51 cases;group C (beating heart group) with 65 cases.During the operation,right atrial appendage muscle was cut off and observed under the electron microscopy.The blood cardiac Troponin T (cTnT) was examined 24 and 72 h postoperation.Results There was no air embolism,death,severe low cardiac output,renal or respiratory failure or other serious complications.One to2 days after surgery,5 cases had mild hemoglobinuria.One-3 days after surgery,7 cases had a temporary atrioventricular separation.There were 2 cases of residual leakage with the diameters both below 2 mm.Two cases in group C suffered from ventricular fibrillation during operation.At 24th h after surgery,there was no significant difference in cTnT ( P ＞ 0.05 ) between group A (0.75 ± 0.25 ) μg/L and group B (0.69 ±0.20) μg/L,and there was statistically great significant difference between group C (0.55 ±0.18) μg/L and groups A or B (P＜0.01).At tge 72nd h after surgery,there was no significant difference in the cTnT level ( P ＞ 0.05 ) between group A (0.53 ± 0.19 ) μg/L and group C ( 0.55 ± 0.13 )μg/L,but there was statistically great significant difference between group A and group B (0.43 ±0.13)μg/L (P ＜0.01 ).The damage in group C was milder,and that in group A was severer.Conclusion Beating heart surgery can significantly reduce the operative duration of VSD repair,thereby it can limit the surgical trauma on the body.Moreover,the method is conservative and practical.In terms of changes of cTnT secretion or myocardial protective effect for repair of VSD,the beating heart surgery was not superior to the hemoperfusion group while both of the groups were superior to the crystalloid group.     

改良负压封闭引流技术治疗糖尿病足的疗效观察

目的:观察应用改良负压封闭引流(VSD)技术治疗糖尿病足的效果.方法:将糖尿病足40例患者随机分为对照组和治疗组.对照组应用传统VSD技术治疗,治疗组应用改良VSD技术治疗,即在VSD敷料中增加一根冲洗管,经冲洗管每日1次注入呋喃西林500 ml和表皮生长因子30 ml,30 min后关闭冲洗管,开启负压.于治疗10 ...     

早期负压封闭引流治疗深Ⅱ度烧伤创面的临床疗效评价

目的 前瞻性评价早期VSD治疗深Ⅱ度烧伤创面的临床疗效,为其临床应用提供依据.方法 选择笔者单位2009年5月-2010年3月收治的双下肢烧伤后3 h内入院、总面积小于10%且各下肢深Ⅱ度面积大于1%TBSA的患者22例.依照部位对称、深度相同、面积相近等同体对照原则,将每例患者创面分为VSD治疗组(应用VSD治疗)与对照组(应用10 g/L磺胺嘧啶银霜换药).观察2组患者创面的水分蒸发量、肿胀程度、细菌定植情况、疼痛程度、愈合时间及愈合质量并进行比较分析.数据行t检验与秩和检验.结果 21例患者完成试验,均在伤后4 h内完成创面处理.VSD治疗组正常皮肤及覆盖敷料前创面的水分蒸发量与对照组相近(t值分别为1.310、-0.911,P值均大于0.05);创面覆盖敷料2 h后,敷料表面的水分蒸发量[(44.3±3.9)mL·h-1·m-2]明显少于对照组[(66.1±6.4)mL·h-1·m-2,t=-11.39,P<0.01].伤后3、7 d,VSD治疗组大腿周径较伤后5 h分别增加了(3.48±0.35)、(2.51±0.21)cm,明显小于对照组的(8.02±0.41)、(3.99±0.32)cm(t值分别为4.110、3.569,P值均小于0.01).2组创面入院时及伤后10 d细菌培养阳性率组间比较,差异均无统计学意义(Z值分别为-0.894、0.000,P值均大于0.05);2组伤后10 d细菌培养阳性率均较各组入院时显著降低(Z值分别为-3.220、-3.870,P值均小于0.01).VSD治疗组创面伤后10 d的pH值(7.12±0.06)呈现弱酸性,对照组(7.41±0.13)则为中性.VSD治疗组伤后1、3、7 d创面疼痛程度轻于对照组(t值分别为-16.132、-21.230、-16.453,P值均小于0.01).2组创面愈合时间比较,差异无统计学意义(t=1.186,P>0.05).伤后2、3个月VSD治疗组创面愈合质量评价为佳(100.00%、100.00%),明显优于对照组(19.05%、85.71%,Z值分别为-11.638、-3.870,P值均小于0.01).结论 早期VSD治疗不能使深Ⅱ度烧伤创面愈合时间提前,但能显著提高其愈合质量,是处理深Ⅱ度烧伤创面的有效方法之一,值得临床关注与进一步研究.     

水胶体敷料在大面积烧伤患者中的应用效果及促进创面愈合机制探讨

目的:分析水胶体敷料在大面积烧伤患者中的应用效果及其促进创面愈合机制。方法:选择笔者医院2018年1月-2019年6月收治的200例大面积烧伤患者,以随机数表法分为采取纱布加压包扎的对照组以及采取水胶体敷料包扎的观察组,各100例,两组均接受Meek植皮术治疗,对两组创面愈合时间、创面细菌定量、炎性细胞因子(C反应蛋白、白介素6、转化生长因子-β1)、血管新生指标(血管内皮生长因子、碱性成纤维细胞生长因子)进行比较。结果:观察组创面愈合时间(19.85±1.15)d,短于对照组(25.50±1.50)d,创面细菌定量(420.25±15.20)cfu/g小于对照组(877.50±15.35)cfu/g,差异有统计学意义(t=29.893、211.667,P均=0.000)。治疗后观察组C反应蛋白(9.25±1.05)mg/L、白介素6(71.10±12.10)ng/L、转化生长因子-β1(9.33±1.17)μg/L,低于对照组(12.11±1.08)mg/L、(84.88±12.82)ng/L、(12.14±1.12)μg/L,差异有统计学意义(t=18.987、77.245、17.349,P均=0.000)。观察组血管内皮生长因子(140.29±1.61)ng/L、碱性成纤维细胞生长因子(88.92±1.18)ng/L,高于对照组(124.38±1.52)ng/L、(79.86±1.24)ng/L,差异有统计学意义(t=71.856、52.929,P均=0.000)。结论:在大面积烧伤患者治疗中水胶体敷料能够通过抑制炎症反应、促进血管新生来促进创面愈合,值得在今后临床治疗工作中推广使用。     

持续封闭负压引流结合三棱针多点打孔对蝮蛇咬伤的治疗效果观察

目的：观察腹蛇咬伤后咬伤部位早期持续封闭负压引流（VSD）结合三棱针打孔法治疗的效果.方法：根据患者中毒时间、病情严重程度采用配伍设计,选取2012年7-8月份我院接诊手部腹蛇咬伤肿胀患者40例,随机分为A组（切开引流组,10例,在咬伤部位行＂＋＂字形切开至皮下层）、B组（切开引流＋VSD组,10例,伤口＂＋＂字形切开,然后行持续VSD）、C组（单纯三棱针打孔组,10例,在咬伤伤口周围用三棱针多点打孔20～30个）和D组（三棱针打孔＋VSD组,10例,在咬伤伤口周围用三棱针多点打孔20～30个,然后行持续VSD）.入院后同时给予抗腹蛇毒血清、抗感染及中医中药等治疗.观察患者患肢肿胀消退时间、伤口部位感染、脓肿、坏死发生率及住院时间.结果：①各组间患肢肿胀消退时间差异显著,D组明显低于A、B、C组[（2.1±1.5）d比（5.0±2.7）d,（3.4±1.7）d,（3.1±3.0）d;P〈0.05];②B、D组无一例出现伤口部位感染、脓肿形成及坏死,与A、C组（20%和40%）比较差异显著（P〈0.05）.③各组间伤口愈合时间及住院时间差异显著,D组均明显低于A、B、C组[（3.5±1.5）d比（7.5±2.5）d,（5.3±1.3）d,（4.7±1.3）d; P〈0.05].结论：腹蛇咬伤后咬伤部位早期三棱针打孔法结合持续VSD可显著缩短患者患肢肿胀消退时间及住院时间.     

双股丝线缝合Nice结固定联合弹性敷贴牵张治疗皮肤软组织缺损

目的探讨双股丝线缝合Nice结固定联合弹性敷贴牵张治疗皮肤软组织缺损的临床疗效。方法回顾性分析2017年8月至2019年4月采用双股丝线缝合Nice结固定联合弹性敷贴牵张治疗的皮肤软组织缺损患者23例,男10例,女13例;年龄(36.5±5.3)岁(范围26~76岁)。缺损大小(60±5.3)cm2(28~96 cm2)。于局麻下对创面进行彻底清创,双股丝线缝合,Nice结固定,创面外用弹性敷贴牵拉固定,每隔3 d换药时进行创面收缩。通过观察和记录创面的愈合级别、愈合率、愈合时间、术后瘢痕温哥华瘢痕量表(Vancouver Scar Scale,VSS)评分评估双股丝线缝合Nice结固定联合弹性敷贴牵张治疗皮肤软组织缺损的疗效。结果手术均在清创室内顺利完成,手术时间(42±10.5)min(范围30~50 min),术中出血量(30±2.5)ml(范围20~60 ml),手术费用(180±11.5)元人民币(范围160~240元人民币)。3例失随访,20例患者得到随访,随访时间(4±2.5)个月(范围3~6个月)。23例患者手术后创面愈合率50%±3.5%(范围40%~56%),20例随访患者术后第3天创面愈合率65%±4.3%(范围53%~75%),术后第6天创面愈合率74%±4.5%(范围65%~80%),术后第9天创面愈合率83%±1.8%(范围76%~85%),术后第12天创面愈合率90%±1.6%(范围84%~95%),术后第15天创面愈合率95%±3.5%(范围94%~98%)。术后随访第42天时20患者创面完全愈合,愈合率100%。创面甲、乙级愈合率95%(19/20),瘢痕VSS评分为4(3,6)分,创面瘢痕优良率80%(16/20)。2例于术后第10天由于缝线切割出现线结部分脱落,二次以Nice结缝合固定。1例感染未完全控制,在线结上覆盖负压封闭引流材料,治疗1周后局部感染控制,再次以Nice结收缩创面至创面愈合。结论双股丝线缝合Nice结固定联合弹性敷贴牵张治疗皮肤软组织缺损具有手术操作简便、手术条件要求低、手术时间短、出血少、费用低、创面愈合级别及愈合率高的优势,适合基层医院应用及推广。