Effects of vitamin B12 and B6 on 2,5-hexanedione-induced neuropathy

The therapeutic effect of Vitamin B₁₂ or Vitamin B₆ on 2,5-hexanedione induced-neuropathy in animals was examined by using our electrodiagnostic technique in order to investigate the mechanism of the development of the neuropathy. Pyridoxal phosphate and two forms of Vitamin B₁₂ were administered to rats intoxicated by the neurotoxin for a period of 18 weeks, and the sensory and motor fiber conduction velocity and the motor distal latency of the tail nerve were periodically determined. None of the groups treated with the therapeutic drugs exhibited a definite improvement in the nerve fiber conduction velocity and motor distal latency of the rat tail nerve, as compared with those of the 2,5-hexane-dione-treated controls.     

Spontaneous age-related peripheral neuropathy in B6C3F1 mice

Peripheral neuropathy, which accompanies aging, occurs during the long-term rearing of laboratory animals. The present study set out to delineate the clinical and functional features of this neuropathy. A total of 200 B6C3F1 female mice, in groups of 5 to 20 mice, were sacrificed and autopsied each week beginning at 5 weeks and continuing to 130 weeks of age. Examination for histopathologic changes was conducted on the dorsal nerve roots, sciatic nerves, peroneal nerves, tibial nerves, plantar nerves and brachial nerve plexuses. At 90 weeks of age or later, peripheral neuropathy, characterized by axonal degeneration and Schwann cell proliferation, were observed mainly in the sciatic nerves, brachial nerve plexus and peroneal nerves. These spontaneous age-related nerve lesions appeared in all animals by 100 weeks of age in all nerves, and increased with increasing age. The nerve lesions were most prominent in the distal sciatic nerve. The rectal and hind-limb surface temperatures, motor nerve conduction velocity, blood glucose and HbA1C decreased with increasing age. Elevation of sorbitol contents in sciatic nerves and reduction of myo-inositol levels were also detected in 120-week-old mice. However, except for blood glycemic parameters, no correlation with peripheral nerve lesions could be demonstrated. Spontaneous hypoglycemia (< 40 mg/dL) persisted throughout the day in a small percentage (< 5%) of animals aged 80 weeks or more; these animals had extensive lesions in the peripheral nerves and showed decreased plasma levels of HbA1C and frucutosamines and increased plasma levels of ketones. These results suggest that spontaneous peripheral nerve disorders which accompany aging might worsen if spontaneous age-related hypoglycemia is also present. Such age-related changes must be taken into consideration in experimental studies performed on mice of this age.     

维生素B6的合成工艺改进

用L-丙氨酸与草酸、乙醇同步进行酯化和酰化制得N-乙氧草酰-L-丙氨酸乙酯,再经环合、脱羧制得4-甲基-5-乙氧基(口恶)唑,最后与2-异丙基-4,7-二氢-1,3-二(口恶)庚英经Diels-Alder反应制得维生素B6,总收率54%.此法成本低,适于工业化生产.     

Chemometrics assisted spectroscopic determination of vitamin B6, vitamin B12 and dexamethasone in injectables

A spectrophotometric method is described and applied to resolve ternary mixtures of the corticosteroid dexamethasone sodium phosphate and the vitamins B6 and B12. It involves multivariate calibration based on partial least-squares regression. The model was built with UV-vis absorption spectra, and was evaluated by cross-validation on a number of synthetic mixtures. Satisfactory results for both artificial and commercial samples were obtained. A spectrofluorometric method was also developed for the determination of B6 in the presence of vitamin B12 and dexamethasone. The results provided by both methods for pharmaceutical formulations were compared successfully. None of the described procedures require sample pre-treatment steps.     

HPLC法同时测定更年灵胶囊中3种成分的含量

目的建立高效液相色谱法同时测定更年灵胶囊中淫羊藿苷、维生素B_1及维生素B_6的含量。方法采用高效液相方法,色谱柱:Ultimate AQ-C_(18)(4.6 mm×250 mm,5μm);流动相:0.04%庚烷磺酸钠溶液(含0.12%的三乙胺,用冰醋酸调节pH值至3.3)(A)-乙腈(B)梯度洗脱;检测波长:270 nm;柱温:20℃;流速:1.0 mL/min。结果淫羊藿苷、维生素B1和维生素B_6分别在4.052~48.624、21.63~255.12和20.50~246.00μg/mL范围内,与其峰面积呈良好的线性关系(r=0.999 8、1.000、1.000,n=6)。平均回收率(n=6)分别为99.37%(RSD=1.08%)、100.93%(RSD=1.70%)和99.34%(RSD=1.76%)。结论该法操作简便,结果准确、重复性好,可用于更年灵胶囊的质量控制。     

B族维生素对终末期肾病神经病变的治疗作用

目的 探讨B族维生素对终末期肾病患者外周神经及自主神经病变的治疗作用.方法 终末期肾病患者30例,行维持性血液透析及透析联合滤过治疗后,仍有外周神经及自主神经病变.给予维生素B1100 mg、B6100 mg、B12 500μg,2次/周.同时常规血液透析,或透析联合滤过,治疗后4、8、12周评估患者外周神经、自主神经症状改善情况,感觉神经传导速度改善情况.结果 经B族维生素治疗4周,患者临床症状、神经传导速度均较治疗前有明显改善(P＜0.05),治疗后12周症状改善更为明显(P＜0.01).结论 B族维生素对尿毒症合并外周神经病变患者有明显治疗作用.     

头孢噻肟钠与维生素B6注射液配伍的稳定性研究

目的研究头孢噻肟钠与维生素B6注射液在5%葡萄糖注射液中配伍的稳定性.方法运用紫外分光-系数倍率法考察头孢噻肟钠与维生素B6注射液配伍后6 h内含量变化情况,同时观察外观并测定pH值.结果头孢噻肟钠的线性范围为16～22 mg·L-1,平均回收率为99.99%,RSD为0.12%(n=5).头孢噻肟钠与维生素B6注射液配伍后3 h内,其配伍液的外观、pH值、含量无显著变化;4 h含量明显下降,5～6 h后配伍液的紫外扫描图谱发生显著变化.结论头孢噻肟钠与维生素B6注射液配伍后3 h内性质稳定,此结果可供临床合理用药时参考.     

依帕司他联合维生素B6治疗糖尿病胃轻瘫的临床研究

目的：应用依帕司他联合维生素B6治疗糖尿病胃轻瘫的疗效观察分析。方法选取我院从2012年12月～2013年11月收治的40例糖尿病胃轻瘫患者做为研究对象。按就诊顺序随机分为治疗组和对照组,治疗组22例,对照组18例。对照组应用莫沙必利治疗,治疗组在应用依帕司他的基础上联合维生素B6治疗。观察对比两组患者消化道症状改善程度及钡餐胃排空测量,临床治疗效果。结果治疗组患者的治疗效果显著优于对照组,差异具统计学意义（P＜0.05）。两组患者不良反应发生情况对比,治疗组少于对照组,差异具统计学意义（P＜0.05）。结论依帕司他联合维生素B6治疗糖尿病胃轻瘫是一种安全,有效的治疗方法。该疗法的应用和推广将为广大的糖尿病胃轻瘫患者带来福音,值得临床推广。     

HPLC测定维生素B6片含量测定方法的探讨

目的：建立一种用HPLC测定维生素B6片含量的方法。方法采用HPLC法,选用色谱Aglient C18;流动相为0.04％庚烷磺酸钠溶液-甲醇（85∶25）（用冰醋酸调pH值至3.5）;检测波长：291nm;流速1mL· min －1。结果维生素B6片在（2.613～60.23μg· mL －1浓度范围内）线性关系良好（r＝0.9997）。精确度为0.16％。平均回收率为99.3％。结论此方法可以避免药物中杂质的干扰,分离效果好,准确率高,专属性强,适合维生素B6片的含量测定。     

甘露聚糖肽联合斑蝥酸钠维生素B6治疗恶性胸腔积液的临床研究

目的探讨甘露聚糖肽联合斑蝥酸钠维生素B6治疗恶性胸腔积液的临床疗效。方法收集2016年1月—2017年1月在溧阳市人民医院进行治疗的恶性胸腔积液患者78例,根据用药的差别分为对照组(39例)和治疗组(39例)。对照组胸腔注入斑蝥酸钠维生素B6注射液,50 m L加入20 m L生理盐水,1次/周;治疗组在对照组的基础上胸腔注入甘露聚糖肽注射液,80 m L加入20 m L生理盐水,1次/周。两组患者均经过4周治疗。评价两组患者临床疗效,同时比较治疗前后两组患者胸水指标和KPS评分变化。结果治疗后,对照组和治疗组的临床总有效率分别为64.10%和84.62%。两组比较差异具有统计学意义(P0.05)。治疗后,两组癌胚抗原(CEA)、胰岛素样生长因子结合蛋白-2(IGFBP-2)、基质金属蛋白酶-9(MMP-9)水平均显著降低,干扰素-γ(IFN-γ)和白细胞介素-2(IL-2)水平显著增高,同组比较差异具有统计学意义(P0.05);且治疗组上述指标水平明显优于对照组,两组比较差异具有统计学意义(P0.05)。治疗后,两组KPS评分均升高(P0.05);且治疗组患者比对照组升高更明显(P0.05)。结论甘露聚糖肽联合斑蝥酸钠维生素B6治疗恶性胸腔积液可有效降低癌胚抗原水平,增强机体免疫功能,具有一定的临床推广应用价值。     

用RP-HPLC法同时测定珍珠维E乳膏中维生素B6和维生素E的含量

目的:建立同时测定珍珠维E乳膏中维生素B6和维生素E的RP-HPLC法。方法:色谱柱为Kromasil-C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-0.2%磷酸溶液,梯度洗脱,检测波长285 nm,流速1.0 ml/min。样品在90%乙醇中用超声破碎仪破碎10 min,然后50℃水浴加热5 min处理。结果:维生素B6和维生素E的线性范围分别为1~50μg/ml(r=0.999 9,n=5)和1~50μg/ml(r=0.999 8,n=5);平均回收率分别为(95.32±0.27)%(n=9)和(96.91±0.85)%(n=9)。3批样品中维生素B6和维生素E的含量分别为标示量的(94.91±0.77)%和(96.87±0.85)%。结论:该方法适用于珍珠维E乳膏中维生素B6和维生素E的含量测定。     

高效液相色谱法测定脑络通胶囊中维生素B6的含量

目的:建立高效液相色谱法测定脑络通胶囊中维生素B6含量的方法,并计算含量均匀度。方法:采用高效液相色谱法,Dikma C18柱(250 mm×4.6 mm,5μm);流动相:甲醇-0.06%庚烷磺酸钠水溶液(含1.2%冰醋酸与0.12%三乙胺)(13∶87);流速:1.0 mL.min-1;检测波长:290 nm;柱温:30℃。结果:维生素B6在0.156~0.780μg范围内呈良好的线性关系,回收率为98.9%,RSD为1.8%。含量均匀度符合《中华人民共和国药典》规定。结论:本方法简便、快速、准确,可用于脑络通胶囊中维生素B6的含量测定。