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1.
体内、外血小板活化时血浆11-去氢-血栓烷B2的改变及其意义 总被引:1,自引:0,他引:1
目的和方法:用花生四烯酸(AA)诱导正常人(n=12)富血小板血浆(PRP)血小板活化。结果:AA组、消炎痛+AA组及对照组血浆11-去氢-血栓烷B2(DH-TXB2)浓度分别为:(51±25、5.0±2.8及54±32)ng/L,三组之间无显著差别;但AA组TXB2与P-选择素浓度均较对照组显著升高(P<001),消炎痛对两者的升高有几乎完全的抑制作用。20例急性期脑梗塞患者血浆DH-TXB2浓度(108~550ng/L)远高于对照组(10~93ng/L),无重叠,但两组之间TXB2与P-选择素浓度均有50%左右重叠。另6例患者服用900mg阿司匹林后血浆DH-TXB2下降43%(P<001),但服药前后TXB2及P选择素浓度无显著改变。结论:DH-TXB2作为TXB2的体内主要酶代谢产物,是反映体内血小板活化的一个较好的指标 相似文献
2.
目的:对照观察CO2激光TMR与机械TMR管道内应用VEGF后心肌管道的热损伤、血管生成及纤维化等形态学变化,探讨TMR结合VEGF应用的方法。方法:采用成年家兔40只,均分A、B两组。分别行CO2激光TMR和机械TMR,同时在两组的管道内注射血管内皮细胞生长因子(VEGF)。术后不同时间对照观察心肌管道的形态学变化。结果:激光TMR管道内存在着严重的热损伤,而机械TMR管道内无任何热损伤迹象;术后2周血管生成达高峰期,激光组新生血管密度/数量为(17.84±0.25)条/10×4倍,机械组为(24.20±0.98)条/10×4倍,P<0.01;术后6~8周新生血管密度/数量激光组为(8.93±0.24)条/10×4倍,机械组为(11.08±0.96)条/10×4倍,P<0.01;管道残迹纤维化的直径,激光组为(1.66±0.04)mm,机械组为(0.66±0.04)mm,P<0.001。结论:从TMR管道内新生血管密度/数量及管道残迹纤维化程度来看,机械TMR结合VEGF的应用方法明显优于CO2激光TMR结合VEGF。 相似文献
3.
羧甲基茯苓多糖对HPBL分泌IL—2,TNF,IL—6,IFN—γ的调节作用 总被引:16,自引:0,他引:16
用CMP培养外周血淋巴细胞(HPBL)24、36、48、72h采样检测的IL-2、TNF、IL-6、IFN-γ效价分别可达13.6±4.3,41.9±2.0,1837.4±464.3,1037.9±211.0U/ml,分别比无CMP的细胞培养对照组的效价高0.8,7.4,0.5,10.9倍(P<0.01),说明CMP具有IL-2、TNF、IL-6、IFN-γ的诱生剂功能。由CMP预处理HPBL后经PHA和/或ConA促诱生组的IL-2、TNF、IL-6、IFN-γ效价分别比无CMP的PHA和/或ConA刺激的相应常规诱生组高1.2~2.8,0.5~1.1、0.5~0.8、0.4~0.6倍(P<0.01),尤以CMP+PHA+ConA促诱生细胞因子效果最佳(P<0.01),说明CMP又具有IL-2、TNF、IL-6、IFN-γ促诱生效应。 相似文献
4.
急性丙型肝炎患者免疫状况的研究 总被引:1,自引:1,他引:1
用间接免疫荧光法、酶联免疫吸附试验(ELISA)及LDH释法,对16例急性输血后丙型肝炎(抗-HCV、HCV-RNA均阳性)患者,分别进行外周血单个核细胞(PBMC)的T细胞亚群计数、T4/T8比值、Tac受体的检测及血清可溶性白细胞介素2受体(sIL-2R)NK细胞活性的测定。并与正常人组比较,经t检验发现,急性丙型肝炎患者的T4亚群所占百分比、T4/T8比值及PBMCTac受体表达均明显低于正常人组(P<0.05),而NK细胞活性、血清sIL-2R明显高于正常人组(P<0.05)。患者的这些免疫状态改变,可能对其发病机理的研究有一定意义。 相似文献
5.
缬草提取物抗心肌缺血再灌注损伤的实验研究 总被引:15,自引:1,他引:14
了解缬草提取物对心肌缺血再灌注损伤的保护作用。方法:大耳白种家兔20只,分为实验组和对照组。结扎冠状动脉左室支1h后松解,观察1.5 h。实验组手术前1h,结扎前10min给予缬草提取物100mg/kg(体重)腹腔注射。观察体表标测心电图、心肌SOD:MDA、TXB_2、6-Keto-PGF_1α、TNF-α、IL-β等指标。结果:心电图:结扎后1h,实验组N-ST、ΣST、N.R明显低于对照组( P< 0 .05);松解复灌后1.5h,实验组N-ST、ΣST明显低于对照组( P< 0.05)。心肌匀浆:实验组XOD、MDA、TNF-α均低于对照组( P< 0.05);实验组6-Keto-PGF_(1α)/TXB_2高于对照组(P<0.05)。实验组IL-1β略低于对照组,但统计学上无显著差异。结论:缬草提取物有抗心肌缺血再灌注损伤的作用。 相似文献
6.
应用单克隆与多克隆双抗体夹心法检测20例扩张型心肌病(DCM)及20例正常人(NC)的血清可溶性白细胞介素2受体(sIL-2R),同时测定了外周血自然杀伤细胞(NK)活性和T淋巴细胞亚群,结果发现DCM患者sIL-2R明显高于NC组(P<0.001),而NK活性明显低于NC组(P<0.001),DCM患者T细胞亚群与NC组比较,CD8降低(P<0.01),而CD4/CD8比值升高(P<0.05)。提示细胞免疫功能紊乱参与DCM的病理过程。 相似文献
7.
体重2.5 ̄3.0kg的日本大耳白兔,分为输精管结扎组(VG)和假手术对照组(SOG)。术后4、8、12、18、22mo经耳静脉采血,检测巨噬细胞(Mψ)培养上清中白细胞介素1(IL-1)活性和外周血单个核细胞(PBMC)培养上清中白细胞介素2(IL-2)、白细胞介素6(IL-6)活性及血浆肿瘤坏死因子(TNF)含量。结果表明:①IL-1活性和TNF含量,术后第8mo VG均高于SOG,术后4、8 相似文献
8.
脆性X综合征(FX)患者的脆性位点(FRAXA)与FMR-1位点一致,脆性断点位于(CGG)n上,而FMR-1突变有两类:(CGG)n重复数突变和点突变(或丢失)。同时表明FMR-15'侧CpG岛甲基化。并介绍TFMR-1(CGG)n突变分子生物学检测方法。 相似文献
9.
芬太尼静脉麻醉下血浆cAMP、cGMP水平的变化 总被引:1,自引:0,他引:1
目的:测定芬太尼静脉麻醉对血浆cAMP、cGMP含量的影响。方法:应用微量输液泵对33例行择期上腹部手术病人诱导后持续恒定静脉内输入芬太尼,3组剂量分别为:Ⅰ组25(μg·kg-1·h-1,n=12);Ⅱ组5(μg·kg-1·h-1,n=13);Ⅲ组75(μg·kg-1·h-1,n=8),芬太尼静脉输入到手术缝皮后停止。分诱导前(T1)、气管插管后(T2)、手术70mn时(T3)、术终(T4)4次采静脉血,用放射免疫法测定cAMP、cGMP含量。结果:插管后、手术70min时及术终与术前比较cAMP、cGMP的含量均无显著差异(P>005);组间比较T4时段cAMP值Ⅲ组较Ⅰ组,cGMP值Ⅱ组、Ⅲ组较Ⅰ组有显著性降低(P<005)。结论:芬太尼可有效地抑制血浆cAMP、cGMP的含量升高。 相似文献
10.
浙江汉族人冠心病患者载脂蛋白B基因的多态性研究 总被引:3,自引:1,他引:3
对103例浙江汉族人载脂蛋白(Apo)B基因XbaⅠ酶切位点,应用限制性片段长度多态性(RFLPs)研究表明:(1)冠心病组中少见X2等位基因的相对频率为0.11,显著高于正常对照组的0.02(P<0.01);(2)X2等位基因的相对频率与冠心病的病变范围无明显关联;(3)冠心病组中具X1X2基因型者与具X1X1基因型者比较,HDL-C及SOD水平明显降低(P分别<0.01,<0.05)。本研究结果表明,ApoB基因XbaⅠ酶切点的RFLPs可能与冠心病的发病有一定关系。 相似文献
11.
Study of the relationships between common fragile sites, chromosome breakages and sister chromatid exchanges 总被引:1,自引:1,他引:1
This paper reports the results of an investigation into therelationship between common fragile sites and sister chromatidexchanges (SCE). Human leukocyte cultures were grown in twodifferent media, one complete (RPMI 1640) and one deficientin folic acid and thymidine (199M). Some of the cultures weretreated with DAPI, a non-intercalating compound which bindspreferentially to the AT bases of DNA and is capable of inducingfragile sites. Bromodeoxyuridine (BrdU) was added to all thecultures for SCE analysis. Chromomycin A3 was used for mappinglesions and SCEs by R-banding. A total of 400 cells was examined.The main results show that: BrdU, probably by re-equilibratingthe unbalanced nucleotide pool of the 199 culture medium, interfereswith the synergism between this culture medium and DAPI in inducingthe expression of fragile sites; the SCE frequency per cellis not increased by DAPI in both culture media, therefore thiscompound does not seem to cause any damage to the DNA and seemsmerely to act by inhibiting the normal condensation of a subsetof fragile sites that possess DAPI-specific base sequences;even in the absence of chromosomal lesions, the fragile sitesare significantly preferred as SCE sites to non-fragile sites,whereas in the presence of a lesion, both fragile and non-fragilesites have the same likelihood of undergoing SCE. All this indicatesthat the presence of a lesion strongly favours SCE formationand that common fragile sites are probably chromosome regionspreferentially damaged during the S phase.
3To whom correspondence should be addressed 相似文献
12.
Chromosome lesions which could be interpreted as "fragile sites" on the distal end of the long arm of the X chromosome were identified during a cytogenetic study of 160 mentally retarded adult males with no apparent cause of their mental retardation and one normal adult female with a family history of fra (X) syndrome. Peripheral blood samples were cultured in either M199 or RPMI 1640 medium with FUdR or BrdU. Metaphases were examined for chromosome lesions or fragile sites on the distal end of Xq and 3 distinct sites were observed: Xq26, Xq27.2, and Xq27.3. Other chromosome lesions at Xq28 were observed and interpreted as nonspecific telomeric structural changes. Chromosome lesions were observed in cells from 14 of the 161 individuals. These included: 5 patients with an Xq26 site, 2 with the recently reported Xq27.2 site, 4 with the Xq27.3 site (characteristic of the fra (X) syndrome), 2 with nonspecific telomeric structural changes, and one individual with 2 lesions (a nonspecific telomeric structural change and an Xq26 site). Additional research is necessary to determine the frequency and clinical significance, if any, of lesions occurring in this region of the X chromosome and to distinguish among heritable fragile sites, constitutive fragile sites, and nonspecific telomeric structural changes. 相似文献
13.
Merlin G. Butler G. Andrew Allen Judy L. Haynes Samuel J. Clark 《American journal of medical genetics. Part A》1990,37(2):250-253
Chromosome lesions which could be interpreted as “fragile sites” on the distal end of the long arm of the X chromosome were identified during a cytogenetic study of 160 mentally retarded adult males with no apparent cause of their mental retardation and one normal adult female with a family history of fra (X) syndrome. Peripheral blood samples were cultured in either M199 or RPMI 1640 medium with FUdR or BrdU. Metaphases were examined for chromosome lesions or fragile sites on the distal end of Xq and 3 distinct sites were observed: Xq26, Xq27.2, and Xq27.3. Other chromosome lesions at Xq28 were observed and interpreted as nonspecific telomeric structural changes. Chromosome lesions were observed in cells from 14 of the 161 individuals. These included: 5 patients with an Xq26 site, 2 with the recently reported Xq27.2 site, 4 with the Xq27.3 site (characteristic of the fra (X) syndrome), 2 with nonspecific telomeric structural changes, and one individual with 2 lesions (a nonspecific telomeric structural change and an Xq26 site). Additional research is necessary to determine the frequency and clinical significance, if any, of lesions occurring in this region of the X chromosome and to distinguish among heritable fragile sites, constitutive fragile sites, and nonspecific telomeric structural changes. 相似文献
14.
目的探讨早孕期胎儿颈项透明层厚度(NT)测量与绒毛染色体核型分析联合应用的临床价值。方法回顾性分析120例早孕期行绒毛染色体核型分析的孕妇,研究其胎儿NT值与染色体核型及妊娠结局的关系。结果 120例病例中胎儿NT≤2.5 mm 76例,未检出异常核型(0%);NT≥2.5 mm 44例,异常核型9例(20.45%)。其中,25例2.5mm≤NT≤3.5 mm的胎儿检出染色体异常1例,检出率为4%(1/25);19例NT≥3.5mm的胎儿检出染色体异常8例,检出率为42.11%(8/19),两组异常核型检出率的差异有统计学意义(P〈0.05)。根据NT增厚程度的不同将NT值分为2.5-3.4mm、3.5-4.4mm、4.5-5.4mm、5.5-6.4mm、≥6.5mm,其中异常核型比例分别为1/25、0/6、2/4、1/2、5/7,各组的差异具有统计学意义(P〈0.05),可以认为不同NT值范围内的染色体异常的检出率不同或不全相同。结论胎儿NT增厚是早孕期筛查胎儿染色体非整倍体异常的有效且敏感的超声指标,绒毛活检行染色体核型分析应作为胎儿NT≥3.5mm的孕妇的首选产前诊断方法。 相似文献
15.
E C Jenkins M S Krawczun S L Stark-Houck C J Duncan S Kunaporn H Gu C Schwartz-Richstein P N Howard-Peebles A Gross S L Sherman 《American journal of medical genetics》1991,38(2-3):447-452
The reliable detection of fra(X)(q27.3) in prenatal samples is important for providing genetic counseling. We have identified 5 new cases of prenatal fragile X [fra(X)] detection in 3 chorionic villus sample (CVS) and 2 amniotic fluid (AF) cell cultures. In 4 of the 5 cases, either excess thymidine (THY) or a combination of THY and 5-fluorodeoxyuridine (FUdR) was clearly superior to FUdR alone as fra(X) inducers. Amniocytes from one case were cultured only in RPMI-1640 and later exposed to FUdR or THY separately. They showed only 2% fra(X) while parallel cultures initiated in Chang medium and incubated in RPMI for at least 7 days (recovery) before fra(X) induction exhibited strikingly increased fra(X) frequencies. Chang medium alone will not allow fra(X) induction in AF (Jenkins EC, Brown WT [1986]: "Genetic Disorders and the Fetus: Diagnosis, Prevention and Treatment." New York: Plenum Press, pp 185-204). Now, using CVS cells, we report that only 1% and 0% fra(X) were detected using FUdR or THY in cells cultured in RPMI for 4 days after removal from Chang medium. Cells with 7 days "recovery" in RPMI exhibited increases from 2 to 6%. Therefore, we have found that Chang medium is very helpful when the appropriate recovery time in another medium is allowed before fra(X) induction. Some false negative reports can be attributed to: induction in Chang medium alone; lack of sufficient recovery time after initiating cells in Chang before induction; and unavailability of the excess THY fra(X) induction system.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
16.
M S Krawczun C J Duncan S L Stark-Houck E C Jenkins 《American journal of medical genetics》1992,43(1-2):149-154
Cytogenetic and molecular protocols for prenatal ascertainment of the fragile X syndrome and the associated fragile site at Xq27.3 are relatively reliable. Any new diagnostic method which becomes available still elicits much interest. Kimchi-Sarfaty et al. [1991] reported an increase in frequency of endoreduplication and polyploidy in fra(X) lymphoblasts and amniocytes when cultured with methotrexate (MTX) or fluorodeoxyuridine. Recently we analyzed the endoreduplication/polyploidy system using amniotic fluid, chorionic villus, and fibroblasts from fra(X) positive abortus cell cultures and from control samples. We observed no increased expression of endoreduplicated or polyploid cells in fra(X) positive amniocytes after exposure to MTX. The data presented here clearly dispute the value of endoreduplication/polyploid scoring as a diagnostic aid in prenatal fra(X) analysis. 相似文献
17.
107例稽留流产胎儿绒毛染色体分析 总被引:3,自引:0,他引:3
目的统计分析胎儿染色体核型异常与稽留流产发生的关系。方法对107例稽留流产孕妇行清宫术时,取胎儿绒毛组织,按常规技术方法制备绒毛细胞染色体,G显带后进行核型分析。结果检测分析流产胎儿绒毛共107例,核型异常64例,异常率为59.81%。其中数目异常58例,染色体结构异常5例。结论染色体异常是稽留流产的重要原因,对稽留胎儿绒毛进行染色体分析可为病因诊断和再生育的优生指导提供可靠依据。 相似文献
18.
目的 探讨人未成熟卵母细胞适宜的培养体系及培养时间.方法 将卵母细胞-卵丘复合体(0CC)332枚和裸卵(DO)393枚随机置入TCM199和P1两种培养体系体外成熟.OCC体外培养48 h,观察其成熟情况.DO分别在24、30、48 h观察第1极体排出情况,计算体外成熟率.OCC和DO成熟后称为成熟的卵母细胞,成熟的卵母细胞行精子卵浆内注射技术(ICSI)受精,比较其在两种培养体系的受精率、卵裂率及囊胚形成率.结果 在TCM199和P1培养体系中,OCC的成熟率、成熟后成为成熟的卵母细胞的受精率和卵裂率的差异无统计学意义(P均>0.05),但成熟的卵母细胞的囊胚形成率为53.7%比37.8%(P<0.05).DO在TCM199和P1培养体系中体外培养24、30、48 h时成熟率的差异无统计学意义;由DO成熟后形成的成熟的卵母细胞在P1培养体系中的受精率、卵裂率和囊胚形成率高于其在TCM199培养体系中,但只有受精率差异具有统计学意义(73.5%比62.9%,P<0.05);DO在两种培养体系中体外培养48 h和30 h的成熟率均明显高于24 h(P<0.05),但48 h与30 h的成熟率相比无差异.结论 OCC适宜在TCM199培养体系的培养;DO适官在P1培养体系的培养.DO体外成熟形成成熟的卵母细胞的时间可以缩短至30 h. 相似文献
19.
Peter Meinecke 《American journal of medical genetics. Part A》1993,45(4):441-442
Myotonic dystrophy (DM) is an autosomal dominant disorder characterized by a trinucleotide (AGC) amplification at 19q13.3. The degree of trinucleotide amplification may increase in successive generations and generally correlates with severity of the disorder. Because amplification of a trinucleotide repeat is also associated with the observation of a fra(X)(q27.3) in the fragile X syndrome, we investigated whether chromosome fragility at 19q13.3 might be inducible in patients with DM. Using 3 different culture stress systems (medium 199, RPMI 1640 with excess TdR, and RPMI 1640 with FudR) and high resolution chromosome analyses, we studied 6 individuals with DM and 5 unaffected relatives representing two unrelated families. Molecular studies were done on two of the most affected patients and showed AGC repeat sequences of 970 and 1,260 at 19q13.3. The normal range of AGC repeat is 5 to 30. We found no indication of fragility at 19q13.3 in any of these individuals. © 1993 Wiley-Liss, Inc. 相似文献
20.
Of 77 patients with bladder carcinoma, 99 tissue specimens--including tissues of patients with recurrent tumors taken after radiotherapy or cytostatics--were subjected to chromosomal analysis. In 42 specimens, recognizable metaphases could be obtained after conventional Giemsa staining and in a smaller number after C- and/or G-banding. All except one had abnormalities of the chromosomes. Short-term cultures for 24-48 hr in RPMI 1640 plus 15% fetal calf serum plus penicillin-streptomycin gave better results than a direct technique (30 min in 0.075 M KCl + 0.1 microgram colcemid/ml at 37 degrees C, followed by fixation). In low stage/grade tumors the number of recognizable metaphases obtained after short-term cultures is lower than in higher stage/grade tissue specimens. 相似文献