聚丁基噻吩的电化学合成及性能研究

丁基噻吩在硝基苯溶液中以六氟磷酸四丁基季铵盐为支持电解质，在恒电流条件下进行电化学氧化聚合，通过电导率的测量及可见──紫外光谱分析，讨论了单体浓度、电解质浓度、电流密度、聚合温度对聚合物膜的导电性能的影响。扫描电镜图表明，随着聚合的进行．膜的表面呈“菜花”状结构。循环伏安图表明聚了基噻吩与聚噻吩具有相近的氧化峰位。     

姜黄素类似物对正常动物细胞和肿瘤细胞间通讯传递的影响

采用正常中国仓鼠肺细胞（Ｖ７９）、Ｂａｌｂ／ｃ小鼠成纤维细胞（Ｂａｌｂ／ｃ－３Ｔ３）、大鼠肝上皮细胞（ＷＢ）、人胚肺细胞（２８Ｓ）观察了间隙连接介导的细胞间通讯功能（ＧＪＩＣ）以及促癌剂ＴＰＡ对ＧＪＩＣ的抑制作用和药物对ＴＰＡ的拮抗作用。结果表明：Ｖ７９、ＷＢ以及３Ｔ３、２ＢＳ细胞均具有中等强度的细胞间隙通讯传导功能，ＴＰＡ对其均呈一定的抑制作用，姜黄素类似物９１０２２、９１０２２－Ｓ对ＴＰＡ具有一定的拮抗作用，即可增强上述４种细胞的通讯传导功能，同时观察到人肺腺癌Ａ５４９和ＧＬＣ无明显间隙连接通讯。９１０２２之所以增强Ａ５４９的传导功能，可能与其具有抗肿瘤作用有关。     

急性分离大鼠新皮层神经元KATP通道特性

为研究急性分离大鼠皮层神经元上ＡＴＰ敏感钾通道的特性,本实验采用了膜片钳技术之膜内面向外记录方法。ＡＴＰ敏感钾通道电导为２００ｐＳ左右,翻转电位为０ｍＶ,有外向整流现象;通道开放常呈簇状猝发样开放,平均开放时间和开放概率随去极化程度增大而增加,随超极化程度增大而减少;低浓度ＡＴＰ有促进通道开放作用,并可激活二级电流;高浓度ＡＴＰ抑制通道开放概率并随浓度增大而增强;ＡＴＰ浓度增高到１或２ｍｍｏｌ／Ｌ时,通道电流被阻断。０.１ｍｍｏｌ／Ｌ优降糖可立即抑制通道活动。急性分离的皮层神经元上ＡＴＰ敏感钾通道特性与培养神经元有差别;低浓度ＡＴＰ可激活ＡＴＰ敏感钾通道,防止神经细胞过度兴奋,从而起到保护脑缺血缺氧损伤的作用。     

腹膜小细胞恶性肿瘤光镜、免疫组化及超微结构观察

获取稳定表达重组人血小板生成素（ｔｈｒｏｍｂｏｐｏｉｅｔｉｎ, ＴＰＯ）的ＮＩＨ３Ｔ３成纤维细胞克隆,为开展成纤维细胞介导的 ＴＰＯ基因治疗研究提供细胞学基础。方法：以重组人ＴＰＯ ｃＤＮＡ为目的基因,构建真核细胞表达重组体ｐｃＤＮＡ３／ＴＰＯ,藉脂质体将其转染于ＮＩＨ３Ｔ３细胞,经Ｇ４１８筛选,获得稳定表达重组人ＴＰＯ的细胞克隆,应用ＰＣＲ、ＲＴ－ＰＣＲ及 Ｗｅｓｔｅｒｎ印迹等技术鉴定目的基因的导入及表达。 结果：ｐｃＤＮＡ３／ＴＰＯ可藉脂质体转染ＮＩＨ３Ｔ３细胞,转染细胞可稳定表达并分泌ＴＰＯ。结论：已获得所需细胞克隆,为应用人ＴＰＯｃＤＮＡ进行基因治疗奠定了基础。     

聚（3—丁基噻吩）电导的热稳定性

研究了导电聚合物聚（３－丁基噻吩）（ＰＢＴ）在大气环境下的导电热稳定性。在室温至２２０℃范围内，测试电导率随时间及温度的变化。ＴＧＡ分析以及化学分析的结果表明：掺杂态的ＰＢＴ大约在１４０℃附近有一转化温度，低于或高于这一转化温度，电导率下降的决定因素各不相同。高于转化温度时，电导率下降的主要原因是反离子和高分子主链的解离，进而发生化学反应，影响了高分子主链的结构。ＸＰＳ测试结果表明：经热处理后的Ｐ     

螯合剂DTPA和BAL对亚慢性镉染毒大鼠促排效果及组织分布的研究

使用螯合剂二乙烯三胺五乙酸（ＤＴＰＡ）和二巯基丙醇（ＢＡＬ）对亚慢性镉染毒大鼠促排效果研究。结果观察到〉Ｗｉｓｔａｒ大鼠采用８０μｇ／ｋｇ镉腹注射４周后，经ＤＴＰＡ８５０ｍｍｏｌ／ｋｇ处理，尿础量增高为对照组的１７２倍，ＤＴＰＡ５４０ｍｍｏｌ／ｋｇ１２次注射后尿镉升高为对照组２０．９７倍。注射９０μｍｏｌ／ｋｇＢＡＬ使尿液中镉排出题 增加了１４．９３倍６０μｍｏｌ／ｋｇ１２次重复注射ＢＡＬ尿镉排出     

聚对苯撑的掺杂和导电性能研究

采用ＡｌＣｌ３－ＣｕＣｌ２催化体系合成了聚对苯撑，并用ＦｅＣｌ３在硝基甲烷溶液中对聚对苯撑进行了掺杂，研究了掺杂浓度，温度，时间和空气中暴露时间对聚对苯撑电导率的影响，结果表明，掺杂后的聚对苯撑具有良好的环境稳定性；在空气中放置３个月电导率基本不变，其电导率约１Ｓ／ｍ，另外，利用红外光谱和紫外光谱研究了掺杂对聚对苯撑结构的影响。     

林可链霉菌噬菌体φSL60启动子的克隆与鉴定

以启动子探针质粒ｐＩＪ４８６为载体，变铅青链霉菌（Ｓ．ｌｉｖｉｄａｎｓ）ＴＫ２３作受体，克隆了林可链霉菌噬菌体ＳＬ６０ＤＮＡ上的启动子活性片段。以ｐＵＣ１８为载体构建ＳＬ６０ＤＮＡ之ＫｐｎＩ片段的基因文库，从中筛选出两个片段，分别克隆于ｐＩＪ４８６中获得ｐＵＩＳ２９和ｐＵＩＳ１９８，在ＭＭ培养基上，两者的转化子对卡那霉素的抗性均仅为１０ｍｇ／Ｌ。噬菌体ＳＬ６０ＤＮＡ的ＢｇｌＩ片段直接克隆入ｐＩＪ４８６的ＢａｍＨＩ位点，获得重组质粒ｐＭＭＳ１，其转化子的卡那霉素抗性在ＭＭ培养基上可达２５０ｍｇ／Ｌ。分析表明ｐＭＭＳ１上的插入片段约为９２０ｂｐ，含有一个ＳｓｔＩ位点，四个ＭｂｏＩ位点。该片段用ＭｂｏＩ部分酶解缩小到６００ｂｐ，亚克隆入ｐＩＪ４８６中得到重组质粒ｐＭＳＢ１４，其转化子对卡那酶素的抗性水平提高到３００ｍｇ／Ｌ。ＳＤＳ－聚丙烯酰胺凝胶电泳显示，ｐＭＳＢ１４中的ｎｅｏ基因可以表达出氨基糖苷磷酸转移酶（ＡＰＨＩ蛋白）。这些结果表明ｐＭＳＢ１４中的插入片段可能具有较强的启动子活性。     

原子转移自由基聚合合成(甲基)丙烯酸酯类模型聚合物及其动力学研究

本文采用一种新颖的活性自由基聚合—原子转移自由基聚合（ＡＴＲＰ）的方法，以１－溴代苯乙烷作为引发剂，过渡金属卤化物与配位剂络合物（ＣｕＢｒ／２，２’－联吡啶）为催化体系，环己酮为溶剂，进行了甲基丙烯酸正丁酯（ＢＭＡ）和丙烯酸正丁酯（ＢＡ）的活性聚合。得到具有指定分子量和窄分子量分布（１．２＜Ｍｗ／Ｍｎ＜１．５）的模型聚合物。计算并讨论了两聚合体系的ＡＴＲＰ的动力学数据     

钙增敏剂MCI—154对内毒素休克大鼠左心室肌原纤维ATP酶 …

研究钙增敏剂ＭＣＩ－１５４对内毒素休克大鼠左心室肌原纤维ＡＴＰ酶活性的影响。方法利用心室肌原是备,测定其在不同钙下浓度的激活液中的ＡＴＰ酶活性。结果内毒休克组心肌肌原纤维在不同ｐＣａ（－ｌｏｇ「（Ｃａ＾２＋）」）溶液中的ＡＴＰ酶活性及最大ＡＴＰ酶活性均明显低于假休克组,ＡＴＰ酶－ｐＣａ曲线向左移位约０．３５个ｐＣａ单位,曲线的中的位值ｐＣａ５０（产生５０％最大ＡＴＰ酶活性所对应的ｐＣａ）明显降低。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.