斑纹芦荟与库拉索芦荟原胶中多糖含量研究

斑纹芦荟与库拉索芦荟原胶中多糖含量研究陈丹包国荣陈卫琳(福建中医学院中药系福州350003)芦荟为库拉索芦荟AloeveraL.及好望角芦荟A.feroxMil或同属植物叶汁的浓缩干燥物,传统主要用于热结便秘、小儿疳积等症。现代研究证实,芦荟除入药外可广泛用于食品和化妆品,主要利用芦荟中具有增强免疫功能的多糖类成分〔1～3〕。有关上述芦荟中多糖成分的研究早有报道,而国产斑纹芦荟A.veraL.var.chinensis(Haw)Berger中的多糖含量分析?...     

芦荟中芦荟苷和芦荟大黄素含量的测定

目的：建立用高效液相色谱法（HPLC）测定芦荟中芦荟苷和芦荟大黄素含量的方法,测定不同芦荟品种中芦荟苷和芦荟大黄素的含量。方法用超声振荡提取法制备样品溶液,HPLC 测定芦荟苷和芦荟大黄素的含量。结果芦荟苷回归方程为 A =13.1784× c -2.8654,r =0.9996,在2.91~46.68 mg / mL 范围内线性关系良好,平均回收率为108.1%,RSD 为2.2%。芦荟大黄素回归方程为 A =49.2886203× c ＋37.246671,r =0.9982,在1.35~21.67 mg / L 范围内线性关系良好。测定的各种芦荟中芦荟苷的含量分别为：华芦荟73.92μg / g、不夜城芦荟2.722μg / g、木立芦荟613.3μg / g、库拉索芦荟136.3μg / g、元江芦荟213.5μg / g。华芦荟中芦荟大黄素含量33.55μg / g,其他品种芦荟中未检出芦荟大黄素。结论不同品种芦荟中芦荟苷的含量不同,木立芦荟中芦荟苷含量最高。所用检测方法简便,准确快速。可作为芦荟中芦荟苷和芦荟大黄素含量的测定方法。     

芦荟多糖含量的比较分析

芦荟为百合科多年生肉质草本植物。常用的主要有3种即库拉索芦荟Aloe .veraL .、木立芦荟A .arborescensMillvar .natalensisBerger .、斑纹芦荟A .veraL .var .chinensis(Haw)Berger。有人对芦荟叶肉组织糖进行了分析[1,2 ] ,并对3个品种的芦荟苷含量进行了测定[4 ] 。但未查阅到有关3个品种间、叶各部分多糖含量的比较,以及1d内不同采收时间对多糖含量的影响。本文对此作出报道,对于合理选择生药材料及生产栽培中确定采集时间....     

4种工艺制备芦荟凝胶中芦荟多糖的含量比较

目的测定比较四种工艺制备的芦荟凝胶中芦荟多糖的含量。方法以D-甘露糖作为对照品,采用紫外分光光度法在610 nm处测吸光度,测定比较鲜榨汁法、冷榨汁法、超声提取法及酶提取法等4种方法所得芦荟凝胶的芦荟多糖。结果甘露糖在0.05~0.60 g·L-1范围内与吸光度呈良好的线性关系(r=0.9985),测得四种工艺制备的芦荟凝胶中芦荟多糖的含量分别为鲜榨汁法2.202 mg·g-1、冷榨汁法1.604 mg·g-1、超声提取法2.000 mg·g-1及酶提取法2.150 mg·g-1。结论不同制备工艺所得芦荟凝胶中芦荟多糖的含量有所不同,其中以鲜榨汁法所得的芦荟多糖含量为最高。研究为芦荟凝胶的制备工艺筛选提供了科学依据。     

芦荟多糖的提取与粗多糖中总糖的含量分析

百合科芦荟属 Aloe植物约 30 0多种 [1 ] ,其中被《中国药典》收载的仅有 2种 ,木立芦荟是日本品种 ,8世纪传入我国 ,一直作为观赏花卉及药用流传于民间 ,《中国药典》尚未收载。芦荟多糖具有增强免疫、抗胃溃疡、抗肿瘤等多种药理活性[2 ,3] ,对《中国药典》所载的库拉索芦荟、好望角芦荟和未收载于《中国药典》的斑纹芦荟中的多糖组成及含量测定有报道[4,5] ,但木立芦荟多糖的研究未见报道。为开发利用芦荟新品种 ,作者从木立芦荟中提取出粗多糖 ,并进行了总糖含量测定。1　仪器和药品72 1型分光光度计 (上海第三分析仪器厂 )。木立芦荟…     

库拉索芦荟多糖对小鼠腹腔巨噬细胞的体外激活作用

 目的　研究库拉索芦荟多糖对小鼠腹腔巨噬细胞的激活作用。方法　用25,50,100,200,400μg·mL^-1的库拉索芦荟多糖体外诱导小鼠腹腔巨噬细胞48h ,测定细胞内乳酸脱氢酶、酸性磷酸酶、精氨酸酶活性及释放的TNF-α和IL-1含量。结果　库拉索芦荟多糖能上调腹腔巨噬细胞内乳酸脱氢酶、酸性磷酸酶和精氨酸酶活性,并能显著诱导腹腔巨噬细胞表达TNF-α和IL-1,增强吞噬中性红能力。结论　库拉索芦荟多糖对小鼠腹腔巨噬细胞具有激活作用。     

中华芦荟多糖的提取

中华芦荟,又名斑纹芦荟、元江芦荟、中国芦荟,来源于百合科植物芦荟,为多年生肉质草本植物,苦、寒、人肝、胃、大肠经。其凝胶内含芦荟甙、芦荟大黄素、芦荟多糖等成分。据报道芦荟多糖具有免疫增强、调节、抗病毒、抗辐射损伤等活性。我们参考有关文献采用水提醇沉法、紫外分光光度法来提取测定中华芦荟多糖的含量,为评价中华芦荟的内在质量提供了依据。     

神奇的芦荟

1、概述：芦荟又名卢会、讷会、象胆、奴会、劳伟。为百合科多年生草本植物库拉索芦荟、好望角芦荟或斑纹芦荟叶中的液汁经浓缩的干燥品。本品原产非洲北部地区,目前于南美洲的西印度群岛广泛栽培,近年来我国南方亦有大量栽培。     

芦荟外用研究概况

芦荟(Aloe Vara)是百合科肉质草本植物,品种多达300种以上,目前使用的药用品种多为库拉索芦荟(Aloe vara L．)、好望角芦荟(A．ferox Mill．)和斑纹芦荟(A．vera L．vat．chinensis Berg．)。芦荟含有酚类、蒽醌类衍生物和多糖等上百种有效成分。近年来国内外学者研究发现其可以用于治疗多种疾病,并且在化妆、美容、饮料、保健品等工农业领域有较广泛的应用,本文就芦荟外用起到的治疗和美容作用作一综述。     

正交试验优选库拉索芦荟多糖的醇沉工艺

目的： 优选库拉索芦荟多糖的醇沉工艺。方法： 以多糖得率为指标,选取浓缩程度、醇沉浓度、醇沉时间为考察因素,通过正交试验优选库拉索芦荟多糖的醇沉工艺。采用硫酸-苯酚法测定芦荟多糖含量。结果： 最佳醇沉工艺条件为新鲜库拉索芦荟多糖提取液浓缩比例1:2,醇沉体积分数70%,醇沉时间6 h;芦荟多糖平均得率达0.076%,平均纯度57.37%。结论： 该优选的工艺条件科学合理,可用于库拉索芦荟多糖的工业化生产。     

Effect of Aloe vera leaves on blood glucose level in type I and type II diabetic rat models

Aloe vera (L.) Burm. fil. (= A. barbadensis Miller) (Liliaceae) is native to North Africa and also cultivated in Turkey. Aloes have long been used all over the world for their various medicinal properties. In the past 15 years, there have been controversial reports on the hypoglycaemic activity of Aloe species, probably due to differences in the parts of the plant used or to the model of diabetes chosen. In this study, separate experiments on three main groups of rats, namely, non-diabetic (ND), type I (IDDM) and type II (NIDDM) diabetic rats were carried out. A. vera leaf pulp and gel extracts were ineffective on lowering the blood sugar level of ND rats. A. vera leaf pulp extract showed hypoglycaemic activity on IDDM and NIDDM rats, the effectiveness being enhanced for type II diabetes in comparison with glibenclamide. On the contrary, A. vera leaf gel extract showed hyperglycaemic activity on NIDDM rats. It may therefore be concluded that the pulps of Aloe vera leaves devoid of the gel could be useful in the treatment of non-insulin dependent diabetes mellitus Copyright 2001 John Wiley & Sons, Ltd.     

库拉索芦荟叶皮层与叶肉组织中芦荟苷和总蒽醌的含量测定

目的 :测定库拉索芦荟叶皮层与叶肉组织中芦荟苷和总蒽醌的含量。方法　高效液相色谱法和紫外 -可见分光光度法。结果　叶皮层中芦荟苷和总蒽醌的含量明显高于叶肉组织 ,鲜品经加热干燥含量明显降低。结论　叶皮层为芦荟的主要活性部位 ,新鲜芦荟在加工过程中应尽量避免加热处理。     

Polypeptides of the Three Major Medicinal Aloes

Major polypeptide species of proteins have been identified and analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) in fresh extracts of the gel portion of mature leaves of Aloe saponaria Haw, Aloe barbadensis Miller, and Aloe arborescens Miller var. natalensis Berger. Extracts of gel from freshly cut Aloe leaves were prepared by dissection, tissue disruption, ethanol extraction, differential centrifugation, and gel filtration methods. Comparisons of SDS-PAGE profiles of Aloe leaf gels showed that all three medicinal Aloes share five major common polypetides. Other results indicated that Aloe saponaria and Aloe barbadensis share the most major polypeptides, i.e. 11, and Aloe barbadensis and Aloe arborescens the least, i.e. 5, while Aloe arborescens had three individual polypeptides not found in the other two species. This is the first report to describe the nature and types of polypeptides detected in extracts of leaf gel portions of mature Aloe plants of these three medicinal species. Accordingly, this report may be of considerable value in helping to identify and characterize those Aloe substances of medicinal importance which are associated with each or all of these medicinal Aloes.     

Hepatoprotective potential of Aloe barbadensis Mill. against carbon tetrachloride induced hepatotoxicity

Aloe barbadensis Mill. Syn. Aloe vera Tourn. ex Linn.(Liliaceae) has been used in variety of diseases in traditional Indian system of medicine in India and its use for hepatic ailments is also documented. In the present study an attempt has been made to validate its hepatoprotective activity. The shade dried aerial parts of Aloe barbadensis were extracted with petroleum ether (AB-1), chloroform (AB-2) and methanol (AB-3). The plant marc was extracted with distilled water (AB-4). All the extracts were evaluated for hepatoprotective activity on limited test models as hexobarbitone sleep time, zoxazolamine paralysis time and marker biochemical parameters. AB-1 and AB-2 were observed to be devoid of any hepatoprotective activity. Out of two active extracts (AB-3 and AB-4), the most active AB-4 was studied in detail. AB-4 showed significant hepatoprotective activity against CCl4 induced hepatotoxicity as evident by restoration of serum transaminases, alkaline phosphatase, bilirubin and triglycerides. Hepatoprotective potential was confirmed by the restoration of lipid peroxidation, glutathione, glucose-6-phosphatase and microsomal aniline hydroxylase and amidopyrine N-demethylase towards near normal. Histopathology of the liver tissue further supports the biochemical findings confirming the hepatoprotective potential of AB-4. The present study shows that the aqueous extract of Aloe barbadensis is significantly capable of restoring integrity of hepatocytes indicated by improvement in physiological parameters, excretory capacity (BSP retention) of hepatocytes and also by stimulation of bile flow secretion. AB-4 did not show any sign of toxicity up to oral dose of 2 g/kg in mice.     

RP-HPLC法测定芦荟中芦荟苷的含量

应用反相ＨＰＬＣ法测定芦荟苷的含量。用ＳＵＰＥＬＣＯ ＬＣ－１８层析柱,流动相：乙腈－０．０１ｍｏｌ／Ｌ三氟醋酸（２２：７８）,流速：１ｍＬ／ｍｉｎ检测波长;３５９ｎｍ,柱温：室温,用外标法测定。芦荟苷的平均回收率为９８．３７％,其检测线性范围为５０￣２５０μｇ／ｍＬ,回归方程Ｃ＝０．２６９＋５４．７２３Ｘ,ｒ＝０．９９９９本法灵敏,简便,重现性好。     

中国芦荟治疗寻常痤疮临床研究

目的：观察中国芦荟治疗寻常痊疮的疗效。方法：全叶组41例外用芦荟全叶，叶肉组40例外用芦荟叶肉，对照组30例外用达芙文凝胶治疗。结果：3组总有效率分别为73．2％，70．0％，76．7％，经统计学处理无显著性差异(P＞0．05)。结论：中国芦荟全叶和叶肉外用治疗寻常痊疮疗效较好，但叶肉更安全，患者依从性好。     

芦荟多糖的纯化工艺与体外抗肿瘤活性

目的:研究芦荟多糖的提取、纯化工艺及体外抗肿瘤活性,为芦荟的产业化开发提供实验依据。方法:采用水提醇沉,三氯乙酸法除蛋白,DEAE-52离子交换色谱分离。用Sephacryl S-400凝胶柱层析纯化并确定芦荟多糖的相对分子量,HPLC法检测纯度,GC法鉴别单糖组成及分子摩尔比。以MTT法观察其体外抗肿瘤活性等。结果:从中华芦荟(Aloe vera L.var. chinesis(Haw.)Berger.)凝胶冻干粉中提取分离得到纯多糖AVP,经HPLC检测为单一对称峰,相对分子量为86000,由甘露糖和葡萄糖组成,摩尔比为10.3∶1.0。AVP对人肝癌细胞(SMMC-7721)、人胃癌细胞(BGC-823)及人肺癌细胞(A549)的增殖均有抑制作用。结论:该法分离的芦荟多糖AVP纯度好,收率高达3.6%,可用于进一步的研究和开发,提高芦荟的资源利用率。     

Effect of Aloe barbadensis Miller Juice on oxidative stress biomarkers in aerobic cells using Artemia franciscana as a model

This study reports on the induction of oxidative stress in aerobic cell systems by Aloe barbadensis Miller (Aloe vera) juice using the salt water crustacean Artemia franciscana as a model. A consistent pattern was observed in which Artemia franciscana nauplii responded to Aloe vera juice exposure with a decrease in the overall activity of redox related enzymes. Exposure of Artemia franciscana to sub‐lethal levels of Aloe vera juice resulted in a decreased activity of thioredoxin reductase, glutathione reductase and glutathione peroxidase by 34% (66% enzymatic activity), 79% (21% enzymatic activity) and 90% (10% enzymatic activity), respectively. Similarly apparent was the trend whereby the co‐exposure of the nauplii to vitamin E counteracted this effect. For each of the biomarker enzymes tested, vitamin E co‐exposure resulted in enzyme activities closer to the control value (78%, 56% and 32% of control enzymatic activities for thioredoxin reductase, glutathione reductase and glutathione peroxidase activity, respectively). These results indicate that exposure to sub‐lethal doses of Aloe vera juice induces alterations in the cellular redox status of Artemia franciscana and that the addition of vitamin E helps the Artemia franciscana nauplii to overcome/block the juice induced oxidative stress. Copyright © 2009 John Wiley & Sons, Ltd.     

中华芦荟组织培养物的芦荟素含量测定

以中华芦荟的根、茎、叶为外植体,探讨了诱导的愈伤组织与芦荟素积累的关系.分别用薄层色谱法和高效液相色谱法测定.结果表明,在MS NAA 1 mg/L 6-BA 0.5 mg/L培养基上,以叶为外植体产生的愈伤组织,分化程度最高,芦荟素含量也高(0.35%);以茎为外植体产生的愈伤组织,芦荟素含量次之(0.08%);以根为外植体产生的愈伤组织不含芦荟素.在MS 2,4-D 1 mg/L 6-BA 0.5 mg/L培养基上,以根、茎、叶为外植体产生的愈伤组织,分化程度低,均不含芦荟素.