Measurement of maximal oxygen uptake from two different laboratory protocols in runners and squash players.

PURPOSE: The aims of the study were to assess whether different test protocols used to elicit maximal oxygen uptake values (VO2max) attain similar results, whether different VO2max protocols were preferable for different athletic groups, and to assess whether the noninvasive criteria used to indicate the attainment of VO2max are achieved similarly in different VO2max testing protocols. METHODS: This study evaluated the attainment of either VO2max or peak VO2 (VO2peak) during two treadmill VO2max protocols: a progressive speed protocol (PSP) and a progressive incline protocol (PIP). Ten runners and 10 squash players were studied to assess whether achievement of VO2max criteria was either sport-specific or protocol-specific, or both. RESULTS: There were no significant differences in the VO2peak values reached in either PSP or PIP protocol (64.4 +/- 5.9 vs 66.5 +/- 6.0 mLO2 x kg(-1) x min(-1)). But HRmax (196 +/- 5 vs 189 +/- 5 beats x min(-1); PSP vs PIP; P < 0.01) and RER (1.14 +/- 0.05 vs 1.07 +/- 0.04; PSP vs PIP; P < 0.01) were significantly higher during the PSP test. Fifty percent of the subjects reached a plateau in either test, and of these subjects, 90% satisfied the three noninvasive criteria for VO2max in the PSP group, compared with 10% in the PIP group. CONCLUSIONS: The indirect criteria used to assess the attainment of VO2max may be limited, as the VO2peak values were higher in the PIP protocol compared with the PSP protocol, although not significantly different, whereas the HR and RER values were significantly lower in the PIP than PSP protocol. Furthermore, only 50% of subjects demonstrated the plateau phenomenon in oxygen uptake with either protocol. It may be concluded that the measured physiological variables coinciding with VO2peak may differ when different protocols are used to elicit VO2max.     

Control of oxygen uptake during exercise

Other than during sleep and contrived laboratory testing protocols, humans rarely exist in prolonged metabolic steady states; rather, they transition among different metabolic rates (V O2). The dynamic transition of V O2 (V O2 kinetics), initiated, for example, at exercise onset, provides a unique window into understanding metabolic control. This brief review presents the state-of-the art regarding control of V O2 kinetics within the context of a simple model that helps explain the work rate dependence of V O2 kinetics as well as the effects of environmental perturbations and disease. Insights emerging from application of novel approaches and technologies are integrated into established concepts to assess in what circumstances O2 supply might exert a commanding role over V O2 kinetics, and where it probably does not. The common presumption that capillary blood flow dynamics can be extrapolated accurately from upstream arterial measurements is challenged. From this challenge, new complexities emerge with respect to the relationships between O2 supply and flux across the capillary-myocyte interface and the marked dependence of these processes on muscle fiber type. Indeed, because of interfiber type differences in O2 supply relative to V O2, the presence of much lower O2 levels in the microcirculation supplying fast-twitch muscle fibers, and the demonstrated metabolic sensitivity of muscle to O2, it is possible that fiber type recruitment profiles (and changes thereof) might help explain the slowing of V O2 kinetics at higher work rates and in chronic diseases such as heart failure and diabetes.     

Reproducibility of the oxygen uptake efficiency slope in normal healthy subjects

BACKGROUND: To elucidate the intertest agreement of the oxygen uptake efficiency slope (OUES) in comparison with those of the maximal oxygen uptake (VO2max) and the ventilatory anaerobic threshold (VAT). METHODS: Experimental design: A comparative study. Setting: Institutional practice. A total of 19 healthy volunteers underwent two sessions of maximal exercise testing with an interval of no more than 7 days. The testing was conducted on a cycle ergometer with the work rate increased by either 20, 30, or 40 Watts (W)/min so that the subject would reach exhaustion within 9 to 12 min of exercise. VAT was defined as the level of oxygen uptake (VO2) at which either an increase in the ventilatory equivalent for oxygen without a concomitant increase in the ventilatory equivalent for carbon dioxide or a change in the slope of the linear relationship between carbon-dioxide production (VCO2) and VO2 occurred. OUES was determined by the following equation: VO2 = a log VE + b, where VE was minute ventilation and "a" was the OUES. Intertest reproducibility was assessed by coefficient of repeatability (COR). RESULTS: The intertest reproducibility of VO2max and OUES were excellent (COR = 570 ml/min [16%] and 740 [20%], respectively). VAT showed poor agreement between the two tests (COR = 650 ml/min [31%]). CONCLUSIONS: Results show that OUES is reproducible and reliable, supporting the clinical usefulness of this index.     

Limitations to maximal oxygen uptake.

An increase in exercise capacity depends on the magnitude of increase in maximum aerobic capacity. Central and peripheral factors may limit oxygen uptake. Central oxygen delivery depends on cardiac output and maximal arterial oxygen content. Peripheral extraction of the delivered oxygen is expressed as a-v O2. With increasing intensities of exercise, the respiratory system may become limiting in some trained individuals. Most studies have shown a higher stroke volume in maximal as well as submaximal exercise in the trained vs untrained individuals. A variety of peripheral factors determine vascular tone. Maximal oxygen uptake depends on all components of the oxygen transporting system, but stroke volume appears to be the prime determinant in the trained subject. At maximum exercise the capacity of the muscle capillary network is never reached.     

Soccer specific testing of maximal oxygen uptake

AIM: Endurance capacity in soccer players is important. A soccer specific test for direct measurement of maximal oxygen uptake does, however, not exist. The aim of this study was to evaluate maximal oxygen uptake in a soccer specific field test, compared to treadmill running. METHODS: Ten male soccer players (age 21.9+/-3.0 years, body mass 73.3+/-9.5 kg, height 179.9+/-4.7 cm) participated in the study, and 5 endurance trained men (age 24.9+/-1.8 years, body mass 81.5+/-3.7 kg, height 185.6+/-3.1 cm) took part in a comparison of the portable and the stationary metabolic test systems. The soccer players accomplished a treadmill test and a soccer specific field test containing dribbling, repetitive jumping, accelerations, decelerations, turning and backwards running. RESULTS: Maximal oxygen uptake was similar in field (5.0+/-0.5 L x min(-1)) and laboratory (5.1+/-0.7 L x min(-1)) tests, as were maximal heart rate, maximal breathing frequency, respiratory exchange ratio and oxygen pulse. Maximal ventilation was 5.4% higher at maximal oxygen uptake during treadmill running. CONCLUSION: These findings show that testing of maximal oxygen uptake during soccer specific testing gives similar results as during treadmill running, and therefore serves as a valid test of maximal oxygen uptake in soccer players.     

Comparison of oxygen uptake on-kinetic calculations in heart failure

PURPOSE: The analysis of oxygen (O(2)) uptake on-kinetics during steady-rate is gaining interest in the heart failure (HF) population. The rate change in O(2) at the initiation of exercise can be assessed via nonlinear regression time constant (TC) or an algebraic equation (mean response time [MRT]). These calculations are presumed to be interchangeable, but research supporting this claim is limited. This investigation compares and contrasts two of the more commonly used O(2) uptake on-kinetic calculations. METHOD: Twenty-eight subjects diagnosed with compensated HF and 19 age, sex, and activity-matched controls underwent a symptom-limited exercise test and a steady-rate exercise session (6 min). Peak O(2) uptake, O(2) uptake at ventilatory threshold, the O(2) uptake TC (TC), and the O(2) uptake mean response time (MRT) were calculated for each subject. RESULTS: O(2) uptake on-kinetics was significantly faster for the control group ( < 0.05) regardless of calculation method. There was a significant difference between the O(2) uptake TC and MRT for the HF group. All O(2) uptake on-kinetic calculations were significantly correlated with aerobic capacity. CONCLUSIONS: O(2) uptake TC and MRT values may not be interchangeable in the HF population. All O(2) uptake on-kinetic calculations did produce a significant difference between experimental and control groups and correlated with indicators of aerobic capacity. The 10-s O(2) uptake on-kinetic calculations may be preferable secondary to expired gas fluctuations associated with breath-by-breath measures. Further work is, however, needed to determine which averaged O(2) uptake on-kinetic expression is optimal given the significant difference between TC and MRT. A mechanism for this difference may be the oscillatory ventilatory expired gas pattern demonstrated by some patients with HF.     

Individual differences in the regulation and degree of maximal uptake of oxygen

Neuronal and hormonal mechanisms responsible for the differences in the maximum oxygen consumption are discussed. The subjects with electroencephalographic and sensory signs of stimulating reticular-hypothalamic-amygdalic effects balanced with inhibitory cortical-striatic-septic-hippocampal-epiphyseal effects showed a high oxygen consumption, moderate excretion of epinephrine and norepinephrine, moderate plasma concentrations of ACTH, cortisol, total and free 11-OHCS and insulin, relatively high concentrations of STH, as well as specific dynamics of hormonal and metabolic reactions to aerobic effects. They included a moderate increase of the excretion of dopamine, DOPA and plasma concentrations of ACTH, a comparatively stable level of cortisol, total and free 11-OHCS, drastic increases of norepinephrine excretion and STH, lactate and pyruvate concentrations, a moderate decrease of insulin and pH levels. The subjects with high hypothalamic-reticular-amygdalic effects exhibited an opposite type of endocrine activity and time-course variations of hormonal-metabolic parameters, as well as low values of oxygen consumption.     

Effect of hyperbaric oxygen on oxygen uptake and measurements in the blood and tissues in a normobaric environment

OBJECTIVE: To examine venous partial pressure of oxygen (PvO(2)), transcutaneous oxygen tension (tcPO(2)), and VO(2)MAX in a normobaric environment after a single hyperbaric oxygen (HBO(2)) treatment. METHODS: This was a prospective study of conditions after the intervention compared with baseline. The participants were 10 moderately trained (VO(2)MAX = 57.6 ml/kg/min) men. Two HBO(2) treatments consisting of breathing 95% oxygen at 2.5 atmospheres absolute (ATA) for 90 minutes were administered on non-consecutive days. Baseline testing included measures of VO(2)MAX, tcPO(2), and anthropometry. At 6.0 (1.0) minutes after the first HBO(2) treatment, a VO(2)MAX test was performed. After the second HBO(2) treatment, leg and chest tcPO(2) and PvO(2) were monitored for 60 minutes. RESULTS: VO(2)MAX, running time, and peak blood lactate were not altered after the HBO(2) treatment. Leg tcPO(2) was lower (p = 0.003) and chest tcPO(2) was unchanged after the HBO(2) treatment compared with baseline values. PvO(2) was significantly (p<0.001) lower in the first three minutes after treatment than subsequent values, but no other differences were found. CONCLUSIONS: A single HBO(2) treatment at 2.5 ATA for 90 minutes does not raise PvO(2), tcPO(2), or VO(2)MAX in a normobaric, normoxic environment.     

Effect of glycogen depletion on the oxygen uptake slow component in humans

Previous studies have indicated that the (.-)VO(2) slow component is related to the recruitment of type II muscle fibres. We therefore hypothesised that an exercise and dietary regimen designed to deplete type I muscle fibres of glycogen would result in a greater contribution of type II muscle fibres to the exercise power output and therefore a larger amplitude of the (.-)VO(2) slow component. Eight male subjects took part in this study. On day 1, the subjects reported to the laboratory at 8 a.m., and completed a 9 min constant-load cycling test at a work rate equivalent to 85 % (.-)VO(2) peak. On day 2 at 12 p.m., the subjects were fed a 4200 kJ meal (60 % protein, 40 % fat); at 6 p.m. they completed a 2 h cycling test at 60 % (.-)VO(2) peak. On day 3 at 8 a.m., the subjects performed an exercise test identical to that of day 1. Metabolic and respiratory measurements indicated that our experimental design was effective in reducing the muscle glycogen content. (.-)VO(2) was significantly higher (by approximately 140 ml x min (-1)) throughout exercise following glycogen depletion but no appreciable changes in (.-)VO(2) kinetics were found: neither the time constant of the primary response (from 35.4 +/- 2.5 to 33.2 +/- 4.4 s) nor the amplitude of the slow component (from 404 +/- 95 to 376 +/- 81 ml x min (-1)) was significantly altered. Therefore, we suggest that the increased (.-)VO(2) throughout exercise and the unaltered (.-)VO(2) slow component following glycogen depletion might be explained by a shift towards a greater reliance on fat metabolism in type I muscle fibres with no appreciable change in fibre type recruitment patterns.     

Acute moderate hypoxia affects the oxygen desaturation and the performance but not the oxygen uptake response

The purpose of this study was to examine the influence of hypoxia on the O2 uptake response, on the arterial and muscular desaturation and on the test duration and test duration at VO2max during exhaustive exercise performed in normoxia and hypoxia at the same relative workload. Nine well-trained males cyclists performed an incremental test and an exhaustive constant power test at 90 % of maximal aerobic power on a cycling ergometer, both in normoxia and hypoxia (inspired O2 fraction = 16 %). Hypoxic normobar conditions were obtained using an Alti Trainer200 and muscular desaturation was monitored by near-infrared spectroscopy instrument (Niro-300). The mean response time (66 +/- 4 s vs. 44 +/- 7 s) was significantly lower in hypoxia caused by the shorter time constant of the VO2 slow component. This result was due to the lower absolute work rate in hypoxia which decreased the amplitude of the VO2 slow component. The arterial (94.6 +/- 0.3 % vs. 84.2 +/- 0.7 %) and muscular desaturation (in the vastus lateralis and the lateral gastrocnemius) were reduced by hypoxia. The test duration (440 +/- 31 s vs. 362 +/- 36 s) and the test duration at VO2max (286 +/- 53 s vs. 89 +/- 33 s) were significantly shorter in hypoxia. Only in normoxia, the test duration was correlated with arterial and muscular saturation (r = 0.823 and r = 0.828; p < 0.05). At the same relative workload, hypoxia modified performance, arterial and muscular oxygen desaturation but not the oxygen uptake response. In normoxia, correlation showed that desaturation seems to be a limiting factor of performance.     

Acute reduction in maximal oxygen uptake after long-distance running

Nine male marathon runners, 24 to 39 years of age, were studied during steady state and maximal graded treadmill exercise under control conditions (C) and immediately after a paced outdoor 21.1-km run averaging 89.5 min (E). The half-marathon run and both treadmill trials were performed at 239 +/- 33 m/min. Oxygen uptake (VO2), respiratory exchange ratio (RER), heart rate (HR), plasma lactate concentration (PLa), and rating of perceived exertion (RPE) were measured in the steady state at 0% grade and at the fatigue end point. Compared to C, mean values in E were significantly lower (p less than 0.05) for time to exhaustion (6.0 vs 4.1 min), VO2max (60.0 vs 56.3 ml/kg/min), peak RER (1.18 vs 1.06), and PLa (9.7 vs 7.8 mM/L), whereas maximal HR (184 vs 184 b/min) and peak RPE (19.6 vs 19.9) were not significantly different between trials. Submaximal VO2 during steady-state runs was similar between C and E (44.4 vs 45.0 ml/kg/min; p = NS). Since the attainable VO2max decreased after E, the percentage of VO2max utilized during steady-state runs was higher, averaging 74% in C and 80% in E (p less than 0.05). In the steady state during E, HR (153 vs 161 b/min) and RPE (13.2 vs 14.8) were higher (p less than 0.05), and the increase in PLa from rest (2.7 vs 1.9 mM/L) was lower (p less than 0.05). Submaximal HR during graded exercise in E was 7 to 8 b/min higher (p less than 0.05) at a given VO2, indicating reduced heart rate reserve.(ABSTRACT TRUNCATED AT 250 WORDS)     

Modeling maximum oxygen uptake of elite endurance athletes

PURPOSE: To compare the maximum oxygen uptake V0(2max) of elite endurance athletes and to explain why the body mass exponent, necessary to render V0(2max) independent of body mass, appears to be greater than 0.67. METHODS: Study 1: V0(2max) of 174 international sportsmen and women was assessed. Athletes were recruited from seven sports (middle- and long-distance runners, heavyweight and lightweight rowers, triathletes, and squash and badminton players). Study 2: calf and thigh leg muscle masses were estimated in 106 male and 30 female athletes from 11 sports. Differences in V0(2max) and leg muscle masses between "sports" and "sex" were analyzed independent of body mass by using allometric log-linear ANCOVA. RESULTS: Heavyweight rowers had the greatest V0(2max) when expressed in L.min but long-distance runners had the highest V0(2max)in mL.kg.min. However, the ANCOVA identified no difference in "mass independent" V0(2max) between the five "pure" endurance sports (runners, rowers, and triathletes) (P > 0.05) with the two racket sports being significantly lower. The body mass covariate exponent was inflated, estimated as 0.94. The results from study 2 estimated calf and thigh leg muscle masses to increase in proportion to body mass, and, respectively. CONCLUSIONS: After having controlled for differences in body mass, V0(2max) did not differ between pure endurance sports (P > 0.05). Assuming that athletes' thigh muscle mass increases in proportion to body mass as observed in study 2, a similar disproportional increase in V0(2max) would be anticipated, providing a plausible explanation for the inflated mass exponent associated with V0(2max) identified in this and other studies.     

Predictors of oxygen uptake and performance during tennis

Tennis ball machine tests permit the concurrent measurement of physiological function and groundstroke performance in a sport specific manner. The purpose of this study was to understand further the demands of groundstroke performance during a test with progressively increasing ball frequency, by determining the running speed between strokes, upper and lower limb acceleration and pulmonary gas exchange throughout. Sixteen tennis players (n = 8, male; n = 8, female; all right handed) completed three 4 min stages of hitting against a ball feed frequency of 15, 20, 25 ball.min(-1) interspersed by 8 min of rest. Stepwise multiple regression analysis identified a predictive model of VO2 containing the variables of left arm acceleration and right ankle acceleration but not running speed (p < 0.0001; adjusted r2 = 0.93; left wrist acceleration Beta = 1.04; right ankle acceleration Beta = - 0.12; S. E. E. = 2.61 ml.kg(-1).min(-1)). Regression analysis found that the strongest predictors of stroke performance (ball speed [m.s(-1)] x stroke accuracy [%]) were right wrist acceleration and stroke economy (p < 0.0001; adjusted r2 = 0.28; right wrist acceleration Beta = - 0.59; movement economy Beta = - 0.28). The findings of this study highlight the contribution of limb acceleration and not running speed to the oxygen cost of tennis groundstroke performance.