透刺联合提肩弹拨推拿疗法治疗肩关节周围炎的临床研究

目的探讨透刺联合提肩弹拨推拿疗法治疗肩关节周围炎的临床疗效。方法选择2007年2月～2009年5月在我院针灸门诊就诊的肩关节周围炎患者146例。按就诊先后顺序随机分为观察组和对照组各73例。①观察组采用肩髑透极泉、肩髂透肩孺、天宗透肩贞、外关透内关,并联合提肩弹拨推拿疗法。②对照组常规针刺肩髑、肩髂、肩贞、肩前、阿是穴。两组患者每日治疗1次,5日为1个疗程,疗程间休息2日,共治疗4个疗程。疗程后采用视觉模拟评分法对患者进行活动性压痛评估,同时检查关节活动度的恢复情况,并对比分析。结果按意向处理分析,146例全部进入结果分析。观察组的总有效率为87．7％,明显优于对照组的71．2％,两组之间总有效率差异显著（P〈0．05）;两组患者治疗后与治疗前活动性疼痛评分及关节活动度比较均具有显著性差异（P〈0．01）;两组患者治疗后活动性疼痛评分及关节活动度比较,观察组明显优于对照组（P〈0．01）。结论透刺联合提肩弹拨推拿疗法治疗肩关节周围炎的临床疗效确切,为肩关节周围炎的临床治疗开辟一种新疗法。     

穴位埋线结合康复训练对脑卒中后肩手综合征早期的疗效观察

目的观察穴位埋线结合康复训练对脑卒中后肩手综合征（SHS）早期的治疗效果。方法将80例脑卒中后SHS早期患者随机分为观察组（穴位埋线结合康复训练）、对照组（仅康复训练）各40例,共治疗4周;比较两组患者治疗前后肩痛程度、手肿胀程度、X线片上肩峰至肱骨头间距（AHI）及肩关节活动度评分。结果治疗4周后,两组患者肩痛的视觉类比评分（VAS）、手肿胀程度、AHI、肩关节活动度评分均明显改善（均P＜0.01）,其中观察组优于对照组（均P＜0.01）。结论穴位埋线结合康复训练干预脑卒中后SHS早期的疗效明显,可在临床推广应用。     

中药穴位热敷疗法治疗脑卒中后肩手综合征

目的：观察中药穴位热敷疗法治疗脑卒中后肩手综合征的效果。方法：64例脑卒中后肩手综合征患者随机分为两组,对照组给予常规治疗,治疗组在常规治疗的基础上,给予中药穴位热敷疗法治疗。结果：治疗组与对照组总有效率比较有统计学意义（P〈0．05）。日常生活能力评分治疗前后比较有统计学意义（P〈0．01）,治疗后治疗组与对照组有统计学意义（P〈0．01）。结论：运用中药穴位热敷疗法治疗脑卒中后肩手综合征有显著疗效。     

缪刺肩痛穴结合康复训练治疗肩手综合征的临床研究

目的：探讨缪刺肩痛穴结合康复训练治疗卒中后肩手综合征的疗效。方法：将80例卒中后肩手综合征患者,按照随机分为缪刺肩痛穴结合康复训练为治疗组（40例）,单纯康复训练为对照组（40例）。比较两组手功能（疼痛,Fugl Meyer评定,上举,外展）的改善情况。结果：治疗后治疗组的手功能评分均高于单纯康复训练治疗组（P〈0．01）。结论：缪刺肩痛穴结合康复训练治疗卒中后肩手综合征疗效优于单纯康复训练。     

靳三针疗法配合功能训练治疗中风偏瘫后肩手综合征的临床观察

【目的】观察靳三针疗法配合功能训练治疗脑卒中后肩手综合征的疗效。【方法】将82例脑卒中后肩手综合征的患者随机分为治疗组42例和对照组40例。治疗组采用靳三针针刺治疗方法结合功能训练,对照组单纯采用功能训练。分别于治疗前后采用疼痛视觉模拟评分法（VAS）对疼痛进行评定,采用简式Fugl—Meyer法进行上肢功能评分,并用测角器测量肩关节主动外展和被动上举的无疼痛范围。【结果】两组治疗后与治疗前比较,均能不同程度地缓解疼痛,改善上肢运动功能和扩大肩关节活动范围（P〈0．05或P〈0．01）,且治疗组的改善作用优于对照组（P〈0．05或P〈0．01）。【结论】靳三针疗法结合功能训练可提高脑卒中后肩手综合征的治疗效果．     

针灸结合康复训练治疗肩手综合征的疗效分析

目的：探讨针灸配合康复训练治疗肩手综合征的临床疗效．方法：肩手综合征患者80例随机分为两组：实验组在康复训练的同时给予针灸治疗,对照组进行康复训练．结果：两组治疗后Fugl—Meyer运动功能评分、肩关节疼痛、肩关节活动度、手部肿胀较治疗前均明显改善（P〈0．05）;治疗后实验组比对照组Vugl—Meyer运动功能评分高13分,肩关节疼痛减少2～3分,肩关节活动度中外展改善最显著,增加54分,手部肿胀减少10mL,实验组明显优于对照组（P〈0．05）．结论：针灸结合康复训练能进一步缓解肩痛、手肿及增加肩关节活动度,提高上肢运动功能,是治疗肩手综合征的有效方法．     

针灸辨证施治配合关节松动术治疗冻结肩80例疗效观察

目的：对照观察针灸辨证施治配合关节松动术对冻结肩的临床疗效。方法：将80例冻结肩患者随机分为治疗组和对照组。治疗组用针灸辨证施治配合关节松动术治疗，对照组只用针灸辨证施治。治疗前、后进行肩关节活动度（ROM）和肩痛症状（VAS）评估。结果：两组患者治疗前后肩关节活动度（ROM）和肩痛症状（VAS）较治疗前差异有显著性意义（P〈0．01），两组间肩关节活动度（ROM）和肩痛症状（VAS）比较差异有显著性意义（P〈0．01），治疗组优于对照组。结论：针灸辨证施治配合关节松动术治疗冻结肩的疗效优于单纯采用针灸辨证施治。     

肩三针结合康复训练对于脑卒中后肩手综合征患者疼痛及运动功能影响

目的：比较肩三针结合康复疗法与单纯康复治疗脑卒中后肩一手综合征的疗效差异。方法：将64例患者随机分为肩三针结合康复组（治疗组）、康复组（对照组）各32例。采用上肢运动功能、疼痛、关节活动度等指标判断其28d后的临床疗效。结果：治疗组总有效率为9616％,明显优于对照组的77．42％（P〈0．05）;肩三针结合康复能够明显改善患者的上肢运动功能、上肢疼痛及关节活动度,与单纯康复比较,差异均有统计学意义（P〈0．05）。结论：肩三针结合康复治疗脑卒中后肩一手综合征可有效缓解肢体疼痛症状,促进患肢运动功能的恢复。     

石蜡疗法应用于肩手综合征的疗效观察

目的：观察石蜡疗法（蜡疗）在脑卒中后出现肩手综合征康复治疗中的疗效。方法：42例患者分为治疗组和对照组,对照组用常规治疗的方法,治疗组为在常规治疗的基础应用蜡疗,比较两组疗效。结果：两组患者关节活动度及疼痛评分均较治疗前有所提高,与治疗前比较,差异有统计学意义（P〈0.001）。结论：蜡疗治疗肩手综合征能有效地缓解疼痛,减轻水肿,改善关节活动度,是一种有效的治疗方法,值得在临床上推广。     

浮针治疗中风后肩手综合征临床观察

目的：观察浮针治疗中风后肩手综合征的临床疗效。方法：将80例患者分为2组,2组均有基础药物治疗,治疗组使用浮针疗法,对照组使用传统电针法,治疗为每周连续治疗5d,休息2d,3周为1个疗程。疗程结束后观察：2组临床疗效、治疗前后2组神经功能缺损程度评分、肩手综合征评估量表及疼痛视觉模拟评分法（VAS）比较。结果：2组临床疗效比较,差异有显著性意义（P〈0.05）,表明浮针组临床疗效优于单纯传统电针组;神经功能缺损程度评分、肩手综合征评估量表评分（SHS）、疼痛视觉模拟评分法（VAS）评分方面浮针组与对照组比较,差异均有显著性意义（P〈0.05）。提示：浮针治疗中风后肩手综合征其临床疗效优于传统电针组。结论：浮针疗法治疗中风后肩手综合征疗效显著。     

益智健脑颗粒联合针灸对阿尔茨海默病大鼠学习记忆的影响

目的观察益智健脑颗粒联合针灸对阿尔茨海默病（Alzheimers disease,AD）大鼠学习记忆的影响。方法将大鼠随机分为假手术组（A组）、模型组（B组）、针灸组（C组）、益智＋针灸组（D组）各10只,B、C、D 3组分别以海马CA1区注射β淀粉样蛋白25-35（Aβ25-35）造模,A组注射等量的双蒸水,各组分别治疗20 d后行Morris水迷宫试验,观察大鼠学习记忆能力变化。结果B组较A组的平均潜伏期明显延长,差异具有统计学意义（P〈0.05）;与B组比较,C组、D组的平均潜伏期明显缩短,过台次数增多,差异具有统计学意义（P〈0.05,P〈0.01）;与C组比较,D组的潜伏期缩短,过台次数增多,差异具有统计学意义（P〈0.05）。结论益智健脑颗粒联合针灸能够提高Aβ25-35介导的AD模型大鼠的学习记忆能力。     

Liuwei Dihuang,a traditional Chinese herbal formula,suppresses chronic inflammation and oxidative stress in obese rats

OBJECTIVE： To investigate the anti-inflammatory, anti-oxidative stress, and adipokine-ameliorating effects of Liuwei Dihuang （LWDH）, a traditional Chinese herbal formula, in obese rats. METHODS： After 2 weeks of acclimation with free access to regular rodent chow and water, obese-prone-caesarean-derived （OP-CD） rats were fed a modified AIN-93G diet containing 60% energy from fat. Treatment was performed twice daily by gavage feeding with 500, 1 500, or 3 500 mg/kg body weight LWDH suspended in water （n=12 rats per group）. Twelve obese-resistant-CD （OR-CD） rats were fed the atherogenic diet and gavaged with water, and served as the normal control. Blood biomarkers of inflammation, oxidative stress and adiponectin were measured post-sacrifice and used to determine the treatment effect of LWDH and assess the suitability of OR/OP-CD rats for studying these parameters. RESULTS： After 9 weeks of treatment, LWDH lowered serum C-reactive protein （CRP） and tumour necrosis factor-α （TNF-α） levels. Serum interleukin-6 （IL-6） levels showed a tendency towards reduction, but were not significantly different from the OP-CD control. Liver superoxide dismutase （SOD） activity was increased in response to all three doses of LWDH, while the levels of reduced （GSH） and oxidized glutathione （GSSG） and thiobarbituric acid reactive substances （TBARS） were unchanged. Serum adiponectin levels were increased in response to oral administration of LWDH at the dose of either 500 or 1 500 mg/kg body weight. In addition, comparisons between OR-CD and OP-CD rats revealed differential, and for some biomarkers, conflicting characteristics of high-fat diet-fed OP-CD rats in reference to obese human subjects in terms of inflammatory and oxidative stress biomarkers and circulating adiponectin levels. CONCLUSION： The results show, for the first time, the anti-inflammatory, anti-oxidative stress and adiponectin-ameliorating effects of LWDH in obese rats. The suitability of the OP-JOP-CD rat model as     

表皮生长因子受体酪氨酸激酶抑制剂在非小细胞肺癌应用的瓶颈——获得性耐药

以表皮生长因子受体酪氨酸激酶抑制剂（epidermal growth factor receptor-tyrosine kinase inhibitor,EGFR-TKI）分子靶向治疗研究为肇始,转化性研究使基础实验和临床实践间的鸿沟迅速填平,改变着人们认识治疗肺癌的视角。无可否认,EGFR-TKI上市后极大地延伸了肿瘤学家治疗肺癌的手段,但不管从临床经验、临床研究数据、分子生物学层面还是文献计量学的角度,     

程丑夫教授论治情志病验案举隅

程丑夫是国家级名老中医,湖南中医药大学第一附属医院主任医师、教授、博士生导师,享受政府特殊津贴专家,出身于中医世家,从医40余年,经验丰富,对于内科系统及疑难杂症的治疗颇有心得,笔者有幸跟师学习,聆听教诲,受益匪浅,现将程师论治情志病的经典验案略陈一二。1思虑伤脾案患者肖某,女,27岁。初诊：2014年5月20日。半年前因婚变后出现忧心忡忡,多思多虑,近1月来反复腹部胀满,刻诊：腹胀,食后为甚,呃逆,无反酸,通气后可减轻,无腹痛,不欲食,夜寐不安,二便调。舌红苔厚白腻,脉弦,BP：110／70mmHg。     

补肾活血方对PCOS大鼠模型卵巢中PAI-1mRNA表达的影响

目的探讨补肾活血方对大鼠PCOS模型卵巢局部纤溶酶原激活物抑制因子-1（PAI—1mRNA）表达的影响。方法选用未成年24d龄SD雌性大鼠60只,随机分为模型组、克罗米酚组、补肾活血方高剂量组、补肾活血方低剂量组、正常对照组5组。用Bogovich法建立大鼠多囊卵巢病理模型。以克罗米酚为对照。用原位杂交法观察补肾活血方对多囊卵巢大鼠局部PAI—1mRNA的影响。结果模型组卵巢局部PAI—1mRNA存在卵泡膜间质细胞显著增高,用补肾活血方高、低剂量与克罗米酚药后,卵巢局部PAI-1mRNA的表达明显降低．差异具有统计学意义（P〈0．05、P〈0．01）。补肾活血方高剂量组与克罗米酚组比较,差异具有显著统计学意义（P〈0．01）;低荆量组与克罗米酚组比较差异无统计学意义（P〉0．05）,补肾活血方高、低剂量组比较,低剂量组卵泡膜间质细胞上PAI-1的基因表达增高更明显,但二者差异无统计学意义（P〉0．05）。结论PAI-1mRNA可能与多囊卵巢综合征的发病机制有关。以补肾活血立法的补肾活血方能降低多囊卵巢大鼠局部PAI—1mRNA的显著增高表达．降低PAI—1mRNA卵巢局部的作用。提示补肾活血方可能通过PAI—1mRNA途径促进卵巢排卵的机制。     

UniPron is A Fully Effective Non-hormonal Reversible Contraceptive in Baboon Model （Papio Anubis）

Objective To determine the safety and efficacy of UniPron as a reversible contraceptive.
Methods Vaginal swabs were obtained before and after UniPron administration, cultured onto appropriate culture media and bacteria identification was done based on type of media used, Gram stain reactions, colony morphology and biochemical tests. Vaginal biopsy tissues were processed using paraffin wax method, stained with hematoxylin and eosin and examined under light microscopy to determine the effect of the product on vaginal tissues. The effect of UniPron on sperm was examined by mixing the product with electroejaculated spermatozoa in vitro at different concentrations. For efficacy studies, male baboons of proven fertility were mated with UniPron treated or untreated females of proven fertility during the fertile stages.
Results All the five females （100%） that were treated with UniPron did not conceive and they regained total fertility when the treatment was stopped while all the controls conceived. At a concentration of 40%, UniPron completely immobilized spermatozoa in an in-vitro system. UniPron mechanism of action was by lowering the vaginal pH and on application in baboon, the pH was lowered for at least 3 h after which it went back to normal.
Conclusions As we plan for a study to test UniPron as a microbicide to prevent STIs including HIV, our current study has established that this novel product is effective in contraception and harmless to vaginal tissues and vaginal microbial flora in a baboon model （Papio anubis）.     

WHO西太区与“世界中医药学会联合会”中医名词术语国际标准比较研究：精、神、气、血、津、液部分

精、神、气、血、津、液是中医理论中6个非常重要的概念，有关术语在《WHO西太区传统医学国际标准名词术语》中一共收录了59条，“世界中医药学会联合会”（以下简称世中联）《中医基本名词术语中英对照国际标准》中收录了58条。血、津、液的内涵较为具体，有一定的物质基础，理解并不困难。翻译上虽有差异，但亦不难统一。精、神、     

Mechanical tests on the reconstructed anterior cruciate ligament fixed with allogenetic cortical bone cross-pin on the femoral side

Background Anterior cruciate ligament reconstruction （ACLR） has developed dramatically in the last century.Now,ACLR has become a reliable and productive procedure.Patients feel satisfied in 〉90% cases.The aim of this study was to evaluate the feasibility of allogenetic cortical bone cross-pin （ACBCP） used as a clinical fixation method in anterior cruciate ligament reconstruction on the femoral side based on biomechanical tests in vitro.Methods The specimens were provided by the bone banks of the First Affiliated Hospital of People＇s Liberation Army of General Hospital from September 2011 to June 2012.Fresh deep frozen human allogenetic cortical bone was machined into cross-pins which is 4.0 mm in diameter and 75.0 mm in length.Biomechanical parameters compared with Rigidfix were collected while cross-pins were tested in double-shear test.The load-to-failure test and cycling test were carried out in a goat model to reconstruct anterior cruciate ligament with Achilles tendon autograft on the femoral side fixed by human 4.0 mm ACBCP and 3.3 mm Rigidfix served as control.Maximum failure load,yield load,and stiffness of fixation in single load-to-failure test were compared between the two groups.Cycle-specific stiffness and displacement at cycles 1,30,200,400,and 1 000 were also compared in between.Results In double-shear test both maximum failed load and yield load of 4.0 mm humanACBCP were （1 236.998±201.940） N.Maximum failed load and yield load of Rigidfix were （807.929±110.511） N and （592.483±58.821） N.The differences of maximum failed load and yield load were significant between ACBCP and Rigidfix,P 〈0.05.The shear strength of ACBCP and Rigidfix were （49.243±8.039） MPa and （34.637±3.439） MPa,respectively,P 〈0.05.In the load-to-failure test ex vivo,yield load and maximum failed load of ACBCP fixation complexity （（867.104±132.856）N,（1 032.243±196.281） N） were higher than those of Rigidfix （（640.935±42.836） N,（800.568±64.890） N,P 〈0.05）.However,s     

New Mechanism of Herb-herb Interaction between Ginseng and Trogopterus Based on CYPs in Rat Livers

The study was designed to investigate the potential mechanism of herb-herb interaction between ginseng and Trogopterus （Trg） based on Cytochrome P450 isozymes （CYPs） in rat livers. We estimated the influence on CYP1A2, CYP2E1, and CYP3A1/2 activity caused by ginseng and Trg used in combination. The CYP1A2 and CYP2E1 enzyme activity were induced by ginseng and Trg used in combination. And this induction effect was caused via inducing CYP1A2 and CYP2E1 protein expression which was supposed caused by inducing the gene expression of CYP1A2 and CYP2E1.     

中医疗法治疗运动性疲劳的研究进展

随着竞技体育的发展，运动员经常承受着大负荷、超强度的体力训练，因此极易产生运动性疲劳。疲劳的出现使肌内压增高，局部缺血，造成氧化代谢、H^＋排出率与pH值降低，血乳酸增高，从而影响肌纤维神经传导速度和肌内收缩力量，减弱了肌肉保护能力。致使较多的冲击力传到骨骼上，故易导致疲劳骨折的发生,严重影响了运动员的训练和比赛成绩，对运动员身心产生不必要的伤害。运动性疲劳消除手段的研究一直是竞技体育工作和运动医学关注和研究的焦点。