Effect of polycation based on alkaloid lupinin-antihepolin and its complex with heparin on DNA synthesis in rat hepatocytes

The effect of the antiheparinate antihepolin on the intensity of replication processes in rat hepatocytes was studied. A single intravenous infusion of polycation caused an approximately 6-fold increase in the intensity DNA synthesis on day 2 postinjection followed by a drop to thebaseline level on days 5–6. If antihepolin was injected in parallel with heparin, DNA synthesis was intensified after just 24 hours. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 6, pp. 627–629, June, 1994 Presented by B. T. Velichkovskii, Member of the Russian Academy of Medical Sciences     

Immunological correlation between morphine tolerance and the effect of naloxone in rats of different age

In 3–6-week-old morphine-sensitive rats, in which morphine injection produced an analgetic effect, the serum titer of antimorphine antibodies 24 h postinjection is less than half that observed in morphine-resistant animals. Administration of naloxone to morphine-sensitive rats induces hyperalgesia and considerably raises the serum titer of antimorphine antibodies. Chronic injections of the same dose of morphine, which cause its analgetic effect to disappear, increase the titer of antibodies in morphine-sensitive rats 2-fold. In morphine-resistant rats naloxone produces an analgetic effect followed by its gradual decay and disappearance in the course of chronic administration. Subsequent administration of morphine induces analgesia, raises the titer of antimorphine antibodies, and lowers the titer of antiidiotypic antibodies. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N ^o 1, pp. 67–70, January, 1996 Presented by K. V. Sudakov, Member of the Russian Academy of Medical Sciences     

Binding of3H-diazepam in rat brain eleven months after termination of corazole kindling

Binding of³H-diazepam in rat cerebellum decreases by 14% (p<0.05) 11 months after termination of kindling and one day after injection of a test dose of corazole (30 mg/kg), while it increases by 19.5% after a single injection of a convulsive dose of corazole (50–75 mg/kg). No changes are found in the cortex. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 2, pp. 135–137, February, 1994 Presented by G. N. Kryzhanovskii, Member of the Russian Academy of Medical Sciences     

The effect of morphine on RNA synthesis in some brain structures of rats with various narcological resistance

Different incorporation of³H-uridine in RNA and increased RNA synthesis after the addition of morphine are demonstrated in all brain structures of resistant rats, as well as in the cortex, nucleus accumbens, griseum centrale, and nucleus ventriculus hypothalami of prone rats. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 1, pp. 100–102, January, 1994     

Early Resistance to experimental plague in animals immunized with recombinant plague vaccine

The protective properties of recombinantSalmonella minnesota R595/pFS1 strain soon after immunization (1–3 days) are studied in a model of experimental mouse plague. Unlike the commercial EV strainYersinia pestis vaccine produced at the Saratov Anti-Plague Institute (Mikrob Research-Manufacturing Conglomerate), the experimental recombinant p preparation affords a high level of protection from the 1st day postvaccination, and surpasses the commercial preparation in such parameters as LD₅₀, mean survival time, and percentage of survivors. By the 21st day the protective indexes of both preparations are comparable. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny,Vol. 119, N ^o 1, pp. 54–57, January, 1995 Presented by A. A. Vorob'ev, Member of the Russian Academy of Medical Sciences     

Effect of a short-term elaboration of a local food-rewarded instrumental response on the protein content in neurons of the hippocampus

The protein content in neurons of areas CA1 and CA3 of the rat dorsal hippocampus is compared by cytointerferometry. The involvement of CA3 neurons is observed both during instrumental response conditioning and at delayed times after training, whereas neurons of the CA1 area are involved only at the earliest stages of conditioned response shaping: at the stage of formation of new motor coordination. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 1, pp. 6–8, January, 1994 Presented by V. S. Rusinov. Member of the Russian Academy of Medical Sciences     

Morphological characteristics of lymphocyte-hepatocyte contacts in various periods of liver regeneration in mice

The histo-and ultrastructure of regenerating murine liver is studied after excision of 2/3 of its tissue. Counts of leukocytes and lymphocytes are found to be increased in the intertrabecular spaces after the operation. Lymphocytes come in close contact with hepatocytes and reticuloendotheliocytes forming microtunnels at the sites of contact. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 2, pp. 176–179, February, 1994     

Inhibition of cataractogenesis in congenitally cataractous mice (cat fraser strain) by an antioxidant from the group of sterically hindered phenols

The phenolic antioxidant catavidan, used to treat heterozygous male Cat fraser mice for 4 months starting from the age of 2 months, inhibited cataract development in these mice significantly (Z=0.063 using the nonparametric Mann-Whitney test) after 2 months of treatment. However, the differences between the treated and control (untreated) mice became less marked toward the end of the third month and disappeared by the end of the fourth. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 9, pp. 256–258, September, 1994 Presented by I. P. Ashmarin. Member of the Russian Academy of Medical Sciences)     

Use of assays for chemiluminescence in the examination of animals exposed to ionizing radiation

Results are presented from cherniluminescence assays performed with samples of whole blood and wool from sheep exposed to gamma radiation in a dose of 200 or 600 R. Assays for barium sulfate-stimulated luminol-dependent chemiluminescence emitted by whole blood of the exposed sheep showed decreased amplitudes of chemiluminescence bursts shortly after irradiation, while assays for the lioluminescence of their wool sampled at different times within a week postirradiation and then stored for six months before the assay demonstrated higher burst amplitudes for samples from the animals irradiated with the higher dose and increases in burst amplitudes with increasing intervals between irradiation and sampling. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N ^o 1, pp. 39–41, January, 1996     

The content of tocopherol and lipid peroxidation products in the tissues of rats with genetically determined hyperproduction of free oxygen radicals

It is shown that at the age of 2–3 months S rats with genetically determined hyperproduction of free radicals have the same content of tocopherol in the heart, epididymal fat, adrenals, liver, and liver mitochondria as Wistar rats but a lower content of plasma tocopherol. At 10–12 months, the tocopherol content in all studied tissues and organs, except the liver, is higher than in young S rats and age-matched Wistar rats. This is regarded as a compensatory response facilitating the stabilization of LPO under conditions of increased free radical formation. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N ^o 3, pp. 282–284, March, 1996     

The effect of extract fromRhodiola rosea on the level of inducible HSP-70 in the myocardium during stress

It is demonstrated that the cardioprotective activity of extract fromRhodiola rosea in stress reaches the maximum 5 days after the first dose. Heat shock proteins appear in the myocardium 3 days after an 8-day administration of the extract. It is believed that these proteins are not an important factor in the cardioprotective effect of the extract. Translated fromByullteen' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N ^o 3, pp. 256–258, March, 1996 Presented by R. S. Karpov, Member of the Russian Academy of Medical Sciences     

Autonomic reactions in patients undergoing total oophorectomy and their changes after human fetal tissue transplant

The effect of transplantation of human fetal tissues on the type of autonomic reactions in women of reproductive age after oophorectomy was investigated. The maximal effect of the therapy was observed 1.5–2 months after transplantation, but by the sixth month all parameters returned to baseline. Transplantation of human fetal tissues is contraindicated for patients suffering from essential hypertension. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N ^o 2, pp. 195–199, February, 1996     

Structural changes in spiny neurons of the rat striatum after a unilateral injection of 6-hydroxydopamine

A decrease in the profile field of bodies and dendrite branching of neurons is revealed. An increase in dendrite lengths and in the area occupied by the bodies of spiny neurons is demonstrated 4 and 12 months postoperation due to the growth of terminal dendritic segments and increased number and length of unbranched dendrites. The positive changes in striatal spiny neurons after injection of 6-hydroxydopamine are indicative of a high level of their plasticity. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol, 117, N ^o 4, pp. 446–448, April, 1994 Presented by O. S. Adrianov, Member of the Russian Academy of Medical Sciences     

RecombinantEscherichia coli strains provide high-level expression of human interleukin-3 and interleukin-4

A number of expression vectors carrying the genes coding for interleukin-3 and interleukin-4 are constructed. The influence of the promoter type, the ribosome-binding site, and the number of plasmid copies on the expression of recombinant proteins and the stability of producing strains is studied.Escherichia coli strains providing a stable high level production of human interleukin-3 and interleukin-4 are obtained. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 1, pp. 83–85, January, 1995 Presented by Yu. A. Romanov, Member of the Russian Academy of Medical Sciences     

The role of the lymphatic system in the changes of electrolyte balance in the febrile reaction

The shifts of the electrolyte level in the lymph and blood are of the same direction, namely, increased. The content of potassium, sodium, and calcium changes only after prolonged fever, while that of magnesium changes after just a single administration of pyrogenal. The lymph level of calcium and magnesium rises more significantly as compared to the blood. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 2, pp. 174–176, February, 1994     

Isoforms of alkaline phosphatase from mouse internal organs after bilateral adrenalectomy

The activity of alkaline phosphatase of female CBA and BALB/c mice is studied after bilateral adrenalectomy. Interstrain differences in enzyme activity are revealed in some organs of the control and experimental animals. The expression of new isoforms of alkaline phosphatase in hypocorticoidism is demonstrated. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N ^o 3 pp. 262–264, March, 1996 Presented by V. P. Kaznacheev, Member of the Russian Academy of Medical Sciences     

Pathomorphology of radiation-induced changes in bronchi in the long term after exposure

Bronchial biopsies were studied in inhabitants from 3 ecologically contrasting regions in the long term after exposure to radiation. It is established that in bronchial biopsies diffuse atrophy of the mucous membrane is prevalent in the bronchial part of the lungs in an ecologically unfavorable region. Under these conditions a marked decrease of metabolic and proliferative reactions is shown in cell populations of the bronchial wall, as is evident from the data of radioautographyin vitro and stereological analysis. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 1, pp. 91–95, January, 1995 Presented by D. S. Sarkisov, Member of the Russian Academy of Medical Sciences     

Differential induction of natural suppressor activity of bone marrow cellsin vitro by different types of tumors

The suppressor activity of bone marrow cells not adhering to plastic is shown to appreciably increase after their 48-hour joint preincubation with mastocytoma P-815 supernatant or WEHI-3 myelomonocytic strain and to be unchanged after incubation with dupernatants of K-2 erythroleukemic strain or Ehrlich's ascitic carcinoma. P-815, WEHI-3, and K-2 tumors are shown to produce factors characterized by colony-stimulating activity. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 8, pp. 184–187, August, 1995 Presented by N. V. Vasil'ev, Member of the Russian Academy of Medical Sciences     

Transplantation of fetal and xenogeneic nervous tissue in Parkinson's disease

A mixture of fetal human brain tissue and of neural primordia of NotchDrosophila melanogaster is transplanted to the ventrolateral thalamic nucleus of patients with Parkinson's disease in stage 3–4. Two kinds of operation are performed. In the first case cryodestruction of the zone of tissue implantation is performed before transplant, and in the second case this procedure is not performed. A stable effect manifested in the disappearance of tremor and rigidity is achieved for both kinds of operation. When a transplant is performed without cryodestruction, the effect does not manifest itself until 2 months postoperation. The patients are followed up for 7–12 months postoperation. No side effects or relapses of Parkinson's disease are observed in this period. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 4, pp. 369–372, April, 1994 Presented by N. K. Permyakov, Member of the Russian Academy of Medical Sciences     

Immuno- and phagocytosis-modulating properties of low doses of B1-aflatoxin and benzene

Benzene or benzene-dissolved B₁-aflatoxin in low doses promotes an increase of the Thy-1⁺ cell count in the bone marrow of mice and an enhancement of the thymusdependent immune response.In vitro aflatoxin and benzene are unable to induce the expression of Thy-1-antigen in bone marrow T-precursors. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 2, pp. 158–160, February, 1994 Presented by S. A. Neifakh, Member of the Russian Academy of Medical Sciences