INFLUENCE ON THE INDUCTION AND ELICITATION OF CONTACT-DERMATITIS IN GUINEA PIGS

In the present experiment the authors investigated whether the previously found influence of exteroceptive stimulation of the extremities on the elicitation of an allergic or secondary reaction of the skin in guinea-pigs to DNCB is elaborated via the toxic or primary reaction. An influence of the stimulation on the primary reaction was found too. The experiment was conducted in two parts: 1) Two experimental groups were stimulated electrically on different schedules, continuous or repetitive. A 48 hour interval between stimulation and application of DNCB was added. The skin reactions were rated by 4 observers on a 5-point rating scale. 2) Two experimental groups were stimulated on the same schedules as above. The corticoid levels were measured at different times after stimulation. The experiment showed that the probability of a skin reaction on the allergic (secondary) contact to a threshold dose was greater for the continuously stimulated group than for the control group. Relationships between corticoid levels and skin reactions were discussed. The hypothesis that stimulation has a direct influence on the allergic contact was supported.     

地高辛标记的小鼠β-1,4-半乳糖基转移酶RNA探针的制备和应用

为了制备小鼠 β-1,4-半乳糖基转移酶 ( β-1,4-galactosyltransferase ,β-1,4-Gal T- )地高辛标记的 RNA探针以探讨β-1,4-Gal T- m RNA在坐骨神经组织的表达定位 ,本研究用提取的小鼠脑总 RNA,采用 RT-PCR方法 ,得到β-1,4-Gal T- 的 DNA片断 ,将其克隆到 p GEM-T载体 ;采用体外转录的方法合成地高辛标记的正、反义β-1,4-Gal T- RNA探针 ;运用点杂交的方法分析标记探针的灵敏度 ;最后应用该探针 ,通过原位杂交的方法 ,分析 β-1,4-Gal T- m RNA在小鼠坐骨神经的表达。结果 :构建了β-1,4-Gal T- /p GEM-T质粒 ,获得了高效价的地高辛标记的正、反义β-1,4-Gal T- RNA探针 ,应用该探针发现 β-1,4-Gal T- 在小鼠坐骨神经髓鞘中有表达。以上结果表明 ,制备的地高辛标记的正、反义 β-1,4-Gal T- RNA原位杂交探针可特异地检测β-1,4-Gal T- m RNA在组织中的表达。本研究为进一步分析β-1,4-Gal T- 在坐骨神经及其它神经组织发育和损伤过程中的表达奠定基础。     

敌枯双对雄性小鼠生殖细胞分裂比率及精原细胞染色体畸变的影响

本文研究丁敌枯双对BALB/C雄性小鼠生殖细胞分裂比率及精原细胞染色体畸变的影响。将实验小鼠随机分为三组,即实验组(敌枯双组),阳性对照组(环磷酰胺组)和阴性对照组(双蒸水组)。结果发现:敌枯双能明显诱发精原细胞多倍体率和裂隙率增加,抑制终变期/中期Ⅰ和中期Ⅱ细胞的减数分裂比率,促进精原细胞有丝分裂比率。实验结果还提示:在遗传毒理检测中亚急性实验是必要的,并对实验结果进行了初步讨论。