912和AK—2123对辐射荷瘤小鼠的内分泌效应

昆明小鼠移植Ｓ１８０肉瘤后５天，行肿瘤局部Ｘ线照射，单次剂量５Ｇｙ，连续５天，照前４０ｍｉｎ腹腔注射９１２（和ＡＫ－２１２３）。结果显示，于照射后７天，注射９１２小鼠血清皮质酮水平即开始降低，照后１７天降低最为明显；而血清睾丸酮水平和睾丸ｃＡＭＰ含量趋于升高。另外，９１２与ＡＫ－２１２３联合应用时，与单独给予９１２的变化一致。提示，９１２具有抑制皮质酮分泌和某种程度的促进睾丸ｃＡＭＰ产生和睾丸酮分     

头部照射对去肾上腺雄性大鼠下丘脑-垂体-性腺轴的影响(摘要)

<正> 研究采用10GyX线头部照射去肾上腺雄性大鼠动物模型,观察血清LH,FSH和睾丸酮(TS)水平及睾丸cAMP含量变化。其结果证实,去肾上腺雄性大鼠垂体-性腺轴功能低下。经10GyX线头部照射后第1天,血清LH和FSH(P<0.01)水平降低,与正常大鼠头部照射后第1天变化基本一致。但是,照射后第     

3.0 Gy X线全身照射后对大鼠下丘脑—垂体—肾上腺皮质系统应激反应的影响

本文报道了3.0 GyX线全身照射后1、3日大鼠下丘脑—垂体—肾上腺皮质系统对应激刺激(一侧后肢骨折)的反应性变化。正常大鼠受应激刺激后10min,血浆β—EP、皮质酮、肾上腺和垂体的cAM P及下丘脑M—EnK水平都明显升高,而下丘脑β—EP含量则显著降低。照射后1日未应激组,血浆β—EP、皮质酮和垂体cAMP水平均显著高于正常对照,下丘脑β—EP也明显低于正常对照。照后1日应激组各参数虽明显高于正常对照,但与未应激组相比,除肾上腺cAMP和下丘脑B—EP水平显著升高外,其余各参数均差异不显著。照后3日未应激组,各参数都回降到接近正常水平。照后3日应激与未应激组相比,6项参数有4项显著增高,其余血浆皮质酮和下丘脑B—EP 2项虽有升高但差异不显著。结果表明,3.0GyX射线全身照射基本上未降低机体对应激的适应能力。     

头部X线照射对去胸腺雄性大鼠神经内分必功能的影响

初生48h内去胸腺的成年雄性大鼠下丘脑-垂-体-性腺及皮质系统功能发生改变。经10GyX线头部照射后2天,除了下丘脑M-Enk含量增加外,血清LH和FSH、血尿睾丸酮和皮质酮、垂体和睾丸cAMP以及下丘脑β-EP和L-Enk含量都有不同程度降低,与正常雄性大鼠头部照射后的变化不一致。这些结果提示,由于去胸腺大鼠影响了神经内分泌功能,不可能产生正常大鼠头部照射后所见到的那些变化。     

下丘脑弓状核区损毁对雄性大鼠生殖内分泌的影响

已知初生期注射谷氨酸钠(MSG)可引起大鼠下丘脑弓状核严重损毁。本研究证实,雄性大鼠生后2及4日经腹腔注射MSG(4mg/每g体重),成年后生殖内分泌出现明显改变。体重、垂体和睾丸重下降。血清LH和FSH水平降低。血清和尿中睾丸酮及垂体cAMP趋于下降,而睾丸组织中cAMP含量未见改变。实验结果表明:下丘脑弓状核与LHRH释放有密切关系,是调控生殖内分泌功能的重要部位。     

大剂量X线头部照射对雄性大鼠生殖内分泌功能的影响(摘要)

<正> 本文报道雄性大鼠受10GyX线头部照射后不同时间下丘脑多巴胺(DA)、血清促性腺激素(LH、FSH)、尿睾丸酮(TS)、睾丸cAMP及垂体和睾丸指数的变化。其结果证实:10GyX线头部照射后DA和促性腺激素变化基本一致;其中特别有意义的是,睾丸cAMP含量变化与血清促性腺激素水平变化非常     

~(60)Coγ线局部照射对大鼠睾丸组织环状核苷酸和血浆睾丸酮水平的影响

为了探讨射线照射后睾丸环状核苷酸和睾丸内分泌的变化规律,了解cAMP和cGMP在睾丸辐射效应中的作用,本文研究了~(60)Coγ线局部照射对大鼠睾丸组织cAMP和cGMP,血浆睾丸酮水平的影响。 材料和方法 一、实验动物和照射条件 采用12～13周龄,体重210～290克末交配的雄性Wistar大鼠。第一批110只,随机分为7组,其中1组(20只)作对照,另6组(15只/组)接受照射。动物麻醉后仰卧     

36GNZ等辐波对小鼠血、肝和脾中环核苷酸含量的影响

以频率36GHz,波长8.3mm的毫米波照射BALB/c小鼠背部,15min/d,连续5d,照后1,3,5,7d,用RIA检测血浆、肝和脾中cAMP及cGMP含量,结果表明:照后3,5,7d血浆中cAMP和cGMP含量明显升高(P<0.01);照后7d肝中cAMP水平升高,cGMP含量减少;而脾中cAMP无明显变化,cGMP含量升高。     

低剂量X线照射对小鼠脑啡肽和内分泌激素的影响(摘要)

<正> 本文报道C_(57)BL/6小鼠75mGy全身x线照射后9小时用SRBC腹腔注射进行免疫。在免疫后不同时间,下丘脑脑啡肽(M-Enk, L-Enk)含量明显降低,血清睾丸酮(TS)水平一直波动在对照组之上,睾丸     

大剂量X线头部照射对三种模型大鼠垂体内分泌功能的影响

本文报道,去胸腺雄性大鼠血清PRL、TSH和GH水平明显降低,而去肾上腺雄性大鼠这3种激素水平有不同程度增高,去睾丸大鼠仅血清PRL水平明显增高。三种动物模型经10GyX射线头部照射后48h,去胸腺和去肾上腺大鼠3种激素水平都有降低趋势,而去睾丸大鼠却略有增加。上述结果提示,3种大鼠模型种经内分泌调节功能已发生异常改变,不能产生正常大鼠头部照射后下丘脑-垂体功能的增强效应。     

Protective effect of panaxatriol on function of hypothalamus-pituitary-testes system in rats irradiated with large dose of X-rays

<正> The present study observed the protective effect of panaxatriol on the function of hy-pothalamus - pituitary - testes system after whole body irradiation with 5 Gy X -rays in male rats. The rats were given panaxatriol intraperitoneally once a day (5mg/day) for 14 days beginning from 24 h before the irradiation. The results showed that hypothalamic L-Enk (P<0. 01) and pituitary (3 - EP(P<0. 05) and testicular cAMP (P<0. 01) contents and serum testosterone level (P<0. 01) were all decreased significantly,while serum FSH level (P<0. 01) increased significantly in single irradiation groups compared with the normal control. However, the above - mentioned indices all approached to the normal control levels in irradiation plus panaxatriol groups. These suggest thhat panaxatriol may weaken the decrement of hypothalamic and pituitary opioid peptide contents, testicular cAMP content and serum TS level and the increment of serum FSH level after the rats being irradiated, and that panaxatriol may have protective fu     

大剂量电离辐射对肾素－血管紧张素－醛固酮系统功能的影响

本研究采用５ＧｙＸ射线全身照射雄性大鼠模型，观察照射后１、２、４和７天血清血管紧张素Ⅰ（ＡＴⅠ）、血管紧张素Ⅱ（ＡＴⅡ）和醛固酮（ＡＬＤ）水平的变化。结果表明，照射后１天血清ＡＴⅠ和ＡＬＤ水平降低，血清ＡＴⅡ水平无变化；照射后２天和４天血清ＡＴⅠ波动在对照组水平，血清ＡＴⅡ水平逐渐增高，而血清ＡＬＤ水平明显低于对照组；照射后７天三者都不同程度高于对照组。提示，大剂量全身照射后初期肾素－血管紧张素－醛固酮系统调节发生紊乱，照射后急期此系统对体内水和电解质失衡产生一种适应性反应。     

低剂量X线全身照射对小鼠甲状腺和肾上腺皮质功能的影响

本文报道,75mGyX线全身照射后4天,引起正常小鼠、SRBC免疫小鼠血浆中皮质酮含量降低,而血浆中 T_3、T_4的含量在照射后4天、7天均未见明显变化。提示75mGy照射后肾上腺皮质功能下调,而甲状腺功能未受影响。     

头部X线照射后大鼠血清中皮质酮含量和脾细胞中糖皮质激素受体变化及其相互关系的研究

给Wistar大鼠以10Gy X射线头部照射后不同时间测定血清皮质酮含量和脾细胞中糖皮质激素受体,结果表明:头照后24h受体数已降到对照组的25％,以后逐渐回升,至第9天恢复到接近正常水平。头照后受体的最大结合容量有降低趋势,但差异不显著,而Kd值显著增大,亲和力降低。皮质酮含量的变化呈双峰曲线,第1天即显著增高,至第9天仍显著增高,皮质酮含量和受体的变化规律不一致。体外实验还证实了1～10倍血清浓度的皮质酮在短时间内不会引起所测定的GCR改变。证明皮质酮的变化虽然可能是引起受体变化的一个原因,但不是主要原因。     

中药川莲治疗小鼠肉瘤S180的实验研究

正宗四川黄莲汤剂治疗小鼠肉瘤Ｓ１８０腹水型瘤株，经体内抗实验观察，将小鼠接种Ｓ１８０瘤株，同部位注射川莲药液治疗，连续观察６５天，用药组小鼠存活率１００％，对照组存活率为０。     

低剂量电离辐射对松果腺调节睾丸酮的影响

目的 :探讨低剂量 X射线全身照射对小鼠松果腺调节睾丸酮 ( TS)的影响。方法 :采用放免分析法检测低剂量电离辐射免疫兴奋效应的动物模型血清 TS。结果 :血清 TS在照射后 4h开始升高 ,8h达峰值 ( P<0 .0 1 ) ,1 2 h降至正常 ,2 4 h再次升高 ( P<0 .0 5 ) ,呈双峰变化趋势。结论 :低剂量电离辐射可引起松果腺调节功能增强 ,进而引起下丘脑 -垂体 -性腺轴功能变化 ,TS水平升高。     

低剂量电离辐射对松果腺调节睾丸酮的影响

目的:探讨低剂量X射线全身照射对小鼠松果腺调节睾丸酮(TS)的影响.方法:采用放免分析法检测低剂量电离辐射免疫兴奋效应的动物模型血清TS.结果:血清TS在照射后4 h开始升高,8 h达峰值(P＜0.01),12 h降至正常,24 h再次升高(P＜0.05),呈双峰变化趋势.结论:低剂量电离辐射可引起松果腺调节功能增强,进而引起下丘脑-垂体-性腺轴功能变化,TS水平升高.     

Anti-tumor effect of pEgr-interferon-γ-endostatin gene-radiotherapy in mice bearing Lewis lung carcinoma and its mechanism

Background Gene-radiotherapy, the combination of gene therapy and radiation therapy, is a new paradigm for cancer treatment. To enhance anti-tumor effect of gene-radiotherapy, in this study we construct a radiationinducible dual-gene co-expression vector pEgr-interferon(IFN)-γ/- endostatin and studied the anti-tumor effect of pEgr-IFN-γ/-endostatin gene-radiotherapy in mice bearing Lewis lung carcinoma and its mechanism.Methods Gene recombinant technique was used to construct dual-gene co-expression plasmid pEgr-IFN-γ endostatin, and single-gene expression plasmid pEgr-IFN-γ and pEgr-endostatin. The plasmids packed by liposome were injected locally into the tumors of the mice, and the tumors were irradiated with 5 Gy X-ray 36 hours later. The tumor growth rate at different time and mean survival period of the mice were observed.Cytotoxic activity of splenic cytotoxic T-lymphocyte ( CTL), natural killer (NK) cell and tumor necrosis factor (TNF)-α secretion activity of peritoneal macrophages of the mice in various groups were evaluated 15 days after irradiation. The intratumor micro-vessel density was evaluated by immunohistochemical staining 10 days after irradiation.Results The tumorgrowthrate of the mice in dual-gene-radiotherapy group was significantly lower than those in control group, 5 Gy group and single-gene-radiotherapy group at different time after gene-radiotherapy, and the mean survival period of which was longer. Cytotoxic activity of splenic CTL, NK and TNF-α secretion activity of peritoneal macrophages of the mice in dual-gene-radiotherapy group were significantly higher than those in control group, 5 Gy X-ray irradiation group and pEgr-endostatin gene-radiotherapy group 15 days after irradiation. The intratumor micro-vessel density of the mice in dual-gene-radiotherapy group was significantly lower than those in control group, 5 Gy X-ray irradiation group and pEgr-IFN-γ/gene-radiotherapy group.Conclusion The anti-tumor effect of dual-gene-radiotherapy was significantly better than that of single-gene-radiotherapy by combining the enhancement of anti-tumor immunologic function induced by IFN-γ/with the antiangiogenesis function of endostatin.     

银耳孢糖增强肿瘤辐射效应的初步探讨

荷S_(180)肉瘤小鼠腹膜腔内注射银耳孢糖(TSP)200mg/kg·d×/d。在第3、5和7天注药后40分钟干荷瘤局部照射X射线5Gy。TSP和照射都能明显抑制肿瘤生长;二者合用的抑瘤率在停药后1、5、10天比照射组提高50.00％、32.52％、6.54%。TSP能提高荷瘤小鼠特別是受照鼠的脾重、脾有核细胞数和胸腺细胞~3H-TdR自发掺入率,并拮抗受照后NK细胞活性的下降。