复方丹参滴丸对心肌炎心律失常和心功能远期疗效的影响

目的：观察复方丹参滴丸对心肌炎心律失常和心功能远期疗效的影响。方法：120例心肌炎患经常规治疗1个月以后，随机分为两组。复方丹参滴丸组60例。给复方丹参滴丸10粒，po,tid，对照组地奥心血康60例。给予地奥心血康200mg,po,tid。2组疗程均为2－6mo。结果：复方丹参滴丸组左心射血分数上升，心律失常改善ST段及T波改善达89－93％，临床有效率93％。地奥心血康组有效率73％。两组比较显差异，P＜0．001。结论：复方丹参滴丸对心肌炎心律失常和心功能远期疗效显，优于地奥心血康。     

地奥心血康对大鼠实验性心肌缺血的保护作用

研究地奥心血康对大鼠实验性心肌缺血的影响。方法：结扎ｗｉｓｔａｒ大鼠左冠状动脉造成急性心肌缺血，设缺血对照组（生理盐水）、地奥心血康组（地奥心血康０．１０ｇ／ｋｇ），阳性药普萘洛尔组（普萘洛尔０．０１ｇ／ｋｇ），各组均在结扎冠脉１５ｍｉｎ后经十二指肠给药，记录给药前后不同时间胸前多导联心电图（９个点），以ΣＳＴ和ＮＳＴ来反映心肌缺血程度和范围，结扎冠脉３ｈ，摘取心脏，用Ｎ—ＢＴ染色确定心肌梗塞面积。结果：地奥心血康０１０ｇ／ｋｇ在３０、６０、１２０、１８０ｍｉｎ使ΣＳＴ分别下降２４％、２９％、３０％、２７％（与对照组比Ｐ＜０．０１、Ｐ＜０．０５）；使ＮＳＴ降低１６％、１７％、１６％、１７％（与对照组比Ｐ＜０．０１）持续１８０ｍｉｎ，使梗塞面积由对照组的１５１４％降到９．６０％（Ｐ＜０．０５）。结论：地奥心血康可降低大鼠实验性心肌缺血程度和缺血范围，减少梗塞面积。     

地奥心血康治疗肾病综合征疗效观察

为探讨地奥心血康对肾病综合征Ⅱ型的临床疗效，将５６例肾病综合征Ⅱ型患者随机分为治疗组，应用地奥心血康１００ｍｇ，每日３次口服，连用６个月，并且合用强的松、对照组仅应用强的松。治疗结果为：治疗组总有效率９６．８８％，对照组７９．１７％，两组经ｕ检验Ｐ〈０．０５，认为地奥心血康对肾病综合征Ⅱ型临床疗效较好。     

地奥心血康治疗冠心病心绞痛临床观察

地奥心血康治疗冠心病心绞痛临床观察沈德娟（山东省临沂市铁十四局四处医院临沂市２７６０００）关键词冠心病；心绞痛；地奥心血康１９９３年以来应用地奥心血康（以下称心血康）治疗冠心病心绞痛６６例，现将观察结果报告如下。１资料与方法１．１观察对象冠心病心绞痛...     

地奥心血康对冠心病患者左室舒张功能的影响

地奥心血康对冠心病患者左室舒张功能的影响王淑贞，韩思英（临沂地区中医医院）（临沂地区体校医务室）关键词地奥心血康；冠状动脉疾病；药物疗法地奥心血康（以下简称心血康）．为近年来治疗心血管病的一代新药．已广泛用于临床。为观察心血康对冠心病患者左室舒张功能...     

基于网络药理学和分子对接研究地奥心血康治疗心脏神经官能症的相关机制

目的 应用网络药理学相关的数据和分子对接探索地奥心血康干预心脏神经官能症的靶点和作用机制,为其临床使用提供理论基础和思路。方法 检索中国知网、万方数据库、维普网、中国生物医学文献服务系统数据库建库至2022年9月中地奥心血康、黄山药、穿山龙、穿龙薯蓣的相关文献,同时查阅有关地奥心血康的著作。基于网络药理学方法,利用中药系统药理学数据库与分析平台,获取地奥心血康主要活性成分和作用靶点;结合Genecards、OMIM、Pharm GKB、TTD基因数据库筛选出心脏神经官能症的相关致病基因,与药物作用基因取交集,构建调控网络,通过网络拓扑计算选出核心基因进行GO分析和KEGG分析,并将药物活性成分与核心基因进行分子对接,结合相关研究分析预测地奥心血康干预心脏神经官能症的作用机制。结果 共获得地奥心血康的主要活性成分14个,预测靶点43个;与心脏神经官能症的交集靶点41个,其中SLC6A2、SLC6A4、MAOA等蛋白可能是干预心脏神经官能症的关键靶点。对关键靶点及对应的活性成分进行分子对接,结果显示,地奥心血康的活性成分和对应的核心靶点具有强烈的结合能力。结论 地奥心血康干预心脏神经官能症...     

地奥心血康治疗冠心病60例疗效观察

应用地奥心血康治疗冠心病６０例，临床总有效率８８．４％，心电图显效率为８．３３％，有效率为４０．０％，说明地奥心血康对缓解冠心病心绞痛及改善心肌缺血疗效显著。     

地奥心血康的药理及临床应用评价

地奥心血康的药理及临床应用评价南宁市第一人民医院朱尚勤地奥心血康（ＤＫ）是从我国特有药用植物中提取的甾体总皂甙精制而成。经药效学实验证明，ＤＫ治疗心血管疾病的有效成分是８种甾体皂甙，其含量在９０％以上，８种甾体皂甙分别是Ｓａｐｏｎｉｎｌ～８，其中Ｓａ...     

穿山龙根茎叶不同生长期薯蓣皂苷元的含量测定

目的：检测不同生长期穿山龙地上部分和地下部分薯蓣皂苷元的含量,以确定其地上部分的开发利用价值。方法：采用RP—HPLC法检测不同生长期穿山龙中薯蓣皂苷元的含量。色谱柱：Discovery C18（250mm×4．6mm×5μm）,流动相：甲醇：水（84：16）,流量：0．8ml／min,柱温：室温,检测波长：203nm。结果：薯蓣皂苷元在0．10-1．0mg／ml浓度范围内线性关系良好（r=0．9999）,平均加样回收率为97．23％,RSD为0．879％（n=5）。地上部分（茎叶果实种子）中薯蓣皂苷元含量在8、9月份较高,分别为2．294％和2．117％;根茎（地下部分）中薯蓣皂苷元含量在9、10月份较高,分别为2．261％和2．576％;结论：穿山龙地上部分采收期应为8、9月份,地下部分采收期应为9、10月份,且地上部分也有较高的开发利用价值。     

地奥心血康与山楂联用对实验性动脉粥样硬化的影响

目的 以心奥心血康为对照，观察地奥心血康与山楂联合用药对兔动脉粥样硬化模型的影响。方法 进行甘油三酯，总胆固醇，低密度脂蛋白，高密度脂蛋白，过氧化脂质，超氧化物歧化酶，血小板聚集率和主动脉内膜斑块面积百分率测定。结果 地奥心血康与山楂联合用药具有调节血脂，降低血清过氧化脂质，提高超氧化物歧化酶活性。抵制血小板聚集，减少动脉内膜脂质斑块形成。结论 地奥心血康与山楂联用药疗效优于地奥心血康单独用药。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.