自身免疫性肝炎患者外周血淋巴细胞亚群的变化

目的 探讨自身免疫性肝炎患者外周血淋巴细胞亚群的分布特点。方法 2011年8月至2013年8月我院收治的50例自身免疫性肝炎患者,常规检测外周血淋巴细胞亚群。结果 自身免疫性肝炎患者CD3⁺CD4⁺T、CD3⁺CD8⁺T、CD3⁺CD4⁺ T/CD3⁺CD8⁺T、CD19⁺B、CD5⁺CD19⁺B、CD5⁺CD19⁺B/CD19⁺B细胞比率分别为（46.952±21.107）%、（33.288±11.308）%、（1.592±0.356）%、（13.510±5.536）%、（8.448±6.089）%、（0.776±0.652）%,均显著高于正常人[（64.739±7.615）%、（25.245±3.076）%、（1.421±0.178）%、（9.940±1.207）%、（2.840±0.915）%、（0.294±0.100）%,P<0.05];AIH患者肝功能指标异常、免疫球蛋白升高,肝组织炎症活动指数升高;AIH患者外周血CD3⁺CD4⁺T、CD3⁺CD8⁺T比率与肝功能指标ALT、AST或HAI评分呈显著的正相关（P<0.05）,但与ALP、γ-GT、TBIL、DBIL无相关性（P>0.05）;B淋巴细胞亚群CD5⁺CD19⁺B细胞比率与肝功能指标中ALT、AST或HAI评分、血清lgG呈显著的正相关（P<0.05）,但与ALP、γ-GT、TBIL、DBIL、lgM、lgA无相关性（P>0.05）。结论 自身免疫性肝炎患者外周血淋巴细胞亚群发生了明显的异常。     

病毒性肝炎患者周围血T淋巴细胞亚群测定的临床意义

本文对１５０例甲，乙型病毒性肝炎病毒，３０例慢性血吸虫病患者和１４０例正常人作周围血Ｔ淋巴细胞亚群，细胞膜表面免疫球蛋白和Ｅ玫瑰花结形成细胞等检测。其结果表明，急，慢性病毒性肝炎患者与正常人比较，除ＥＲＦＣ明显较低外，其周围血ＯＫＴ３，ＯＫＴ４，ＯＫＴ８和ＳｍＩｇ均明显增高，尤以急性乙肝患者和血清ＨＢＶ－ＤＮＡ及ＨＢｅＡｇ阳性者更为明显。ＯＫＴ４／ＯＫＴ８比值下降，与对照组比较均有显著性差异。     

初治和复治肺结核患者外周血淋巴细胞亚群的变化及其临床意义

目的比较初治肺结核与复治肺结核患者外周血淋巴细胞亚群比例的变化及其临床意义。方法采用流式细胞仪抗体双标法测定外周血中淋巴细胞亚群的变化,并进行统计学分析。结果与健康对照组相比,初治肺结核患者外周血中B淋巴细胞和CD_4~(++)CD~+_(28)/CD_3~+比例显著高于对照组,NK细胞水平显著低于对照组(P0.05);T淋巴细胞、CD_4~+T淋巴细胞、CD_8~+T淋巴细胞、CD_4~+/CD_8~+比值、CD_8~+CD~+_(28)/CD_3~+、CD_8~+CD~-_(28)/CD_3~+和CD_4~+CD~-_(28)/CD_3~+无明显变化(P0.05)。与健康对照组相比,复治肺结核患者外周血中CD_8~+CD~+_(28)/CD_3~+、CD_8~+CD~+_(28)/CD_3~+和CD_4~+CD~+_(28)/CD_3~+比例显著高于对照组(P0.05、P0.01),而CD_4~+CD~+_(28)/CD_3~+显著低于对照组(P0.01);B淋巴细胞、T淋巴细胞、NK(Natural killer)细胞、CD_4~+T淋巴细胞、CD_8~+T淋巴细胞、CD_4~+/CD_8~+比值则无明显变化(P0.05)。复治肺结核患者外周血中CD_8~+CD~+_(28)/CD_3~+和CD_4~+CD~+_(28)/CD_3~+比例显著低于初治肺结核组(P0.05、P0.01),而CD_8~+CD~+_(28)/CD~+和CD_4~+CD~+_(28)/CD_3~+比例显著高于初治肺结核组(P0.05、P0.01)。与无空洞的初治肺结核患者相比,有空洞的初治肺结核患者外周血中B_3淋巴细胞明显降低(P0.05),与无空洞的复治肺结核患者相比,有空洞的复治肺结核患者外周血中CD_4~+CD~+_(28)/CD_3~+细胞明显降低(P0.05)。结论初治和复治肺结核患者体内存在不同程度的淋巴细胞亚群变化并,其中T淋巴细胞与NK细胞比例与肺结核的进展有一定的关联性。     

风湿性心脏病患者淋巴细胞亚群变化及其临床意义

本文对30例风心病患者T淋巴细胞亚群的变化进行了研究,结果为:风湿活动组周围血淋巴细胞绝对数显著增加,T抑制细胞亚群明显减少,T辅助细胞与T抑制细胞比值显著升高。慢性心衰组T抑制细胞亚群明显增加,T辅助细胞与T抑制细胞比值显著降低。文中还讨论了上述改变的临床意义。     

T淋巴细胞亚群在急性甲型病毒性肝炎中的临床意义

1992年1～4月,我们共收治67例急性甲型病毒性肝炎患儿。对其中的28例,采用单克隆抗体进行T淋巴细胞检测。结果报道如下。 一、临床资料 依据1990年全国病毒性肝炎防治方案,28例患儿全部诊断为急性甲型病毒性肝炎。男16例,女12例,平均年龄5.5岁。     

小儿重型肝炎患者外周血T淋巴细胞亚群的变化与临床意义

目的了解小儿与成人重型肝炎外周血CD3^＋、CD3^＋CD4^＋及CD3^＋CD8^＋T淋巴细胞数量变化的差别,探讨小儿重型肝炎患者外周血T淋巴细胞亚群数量变化与临床意义。方法对35例小儿及30例成人重型肝炎进行外周血CD3^＋、CD3^＋CD4^＋及CD3^＋CD8^＋T淋巴细胞数量检测。结果小儿重型肝炎成活的患儿外周血CD3^＋、CD3^＋CD4^＋及CD3^＋CD8^＋T淋巴细胞数量明显高于死亡组（P〈0．01）：小儿重型肝炎中1岁以内患儿外周血CD3^＋、CD3^＋CD4^＋及CD3^＋CD8^＋T淋巴细胞数量明显高于2～6岁组（P〈0．01）;小儿重型肝炎组CD3^＋、CD3^＋CD4^＋及CD3^＋CD8^＋T淋巴细胞数量明显高于成人重型肝炎组（P〈0．01）;1岁以内的患儿病死率最高（P〈0．05）。结论／JxJL与成人重型肝炎外周血CD3^＋、CD3^＋CD4^＋及CD3^＋CD8^＋T淋巴细胞数量有明显差异,年龄越小,CD3^＋、CD3^＋CD4＋及CD3^＋CD8^＋T淋巴细胞数量越高;dxJL细胞免疫与成人明显不同,年龄越小,病情越重;病死率越高。     

自身免疫性肝病患者外周血淋巴细胞亚群的频率变化及临床意义

目的调查原发性胆汁性肝硬化(PBC)、自身免疫性肝炎(AIH)及AIH-PBC重叠综合征患者外周血淋巴细胞亚群频率变化及其临床意义。方法本中心2001年6月-2010年12月期间,对41例AIH-PBC、37例AIH和36例PBC患者,以及50例健康人群外周血进行淋巴细胞亚群频率检测。分析患者年龄、性别、肝功能、是否肝硬化及淋巴细胞亚群频率的变化。结果与健康组相比,AIH-PBC重叠综合征组、PBC组和AIH组的CD3+CD4+T细胞频率显著升高,而CD3-CD16+CD56+NK细胞频率显著降低;PBC组和AIH-PBC重叠综合征中CD4+/CD8+比值、CD3-CD19+B细胞频率偏高,CD3+CD8+%T细胞频率降低。在疾病发展的不同阶段,AIH-PBC重叠综合征组和PBC组中,肝硬化组CD3+%T细胞频率、CD3+CD8+%T细胞频率较非肝硬化组偏低。结论通过回顾性分析健康人群和AIH、PBC及AIH-PBC重叠综合征患者淋巴细胞亚群的分布特点及其与疾病进展的关系,为临床科学评价上述自身免疫性肝病人群的免疫状态提供重要的免疫指标。     

急性髓系白血病患者外周血淋巴细胞亚群变化的临床意义

目的 分析探讨免疫因素T淋巴细胞水平在急性髓系白血病中的意义。方法 收集初诊为急性髓系白血病的46例患者(除外急性早幼粒细胞白血病(acute promyelocytic leukemia, APL)为实验组,给与标准的化学治疗,根据治疗效果分为完全缓解组(CR)32例,未缓解组(NR)14例;并在健康体检人群中选择43人作为本次研究的对照组,观察实验组患者中首次化疗前、后的T淋巴细胞亚群变化情况。结果 实验组CD3⁺、CD4⁺,通过计算得出CD4⁺与CD8⁺比值,均低于健康对照组,差异有统计学意义(P<0.05);CD8⁺高于健康对照组,差异有统计学意义(P<0.05)。治疗后CD3⁺、CD4⁺,通过计算得出CD4⁺与CD8⁺的比值,均明显升高,差异有统计学意义(P<0.05);CD8⁺数量降低,差异无统计学意义(P>0.05)。完全缓解组的C...     

乙型病毒性肝炎患者T淋巴细胞亚群的测定与其临床意义分析

目的研究各型乙型病毒性肝炎患者外周血T淋巴细胞亚群的变化,以及在病毒复制状态下免疫功能的紊乱情况。方法应用流式细胞仪检测T淋巴细胞亚群,荧光定量PCR法测定HBV-DNA。结果慢性乙型病毒性肝炎患者CD4+/CD8+值较正常对照组下降。肝硬化组与正常组无显著性差异。在乙型肝炎病毒复制组与不复制组CD4+/CD8+有显著性差异。结论乙型病毒性肝炎为免疫损伤所致,在慢性肝炎及乙型肝炎病毒复制状态下存在严重免疫功能紊乱。     

Usefulness of liver infiltrating CD86-positive mononuclear cells for diagnosis of autoimmune hepatitis

AIM: Although the pathogenic mechanism underlying autoimmune hepatitis (AIH) remains unclear, the immune system is thought to be critical for the progression of the disease. Cellular immune responses may be linked to the hepatocellular damage in AIH. Recently, much attention has been focused on the critical functions of costimulatory molecules expressed on mononuclear cells in the generation of effective T cell-mediated immune responses. Analysis of costimulatory molecule expressed on mononuclear cells from the patients with AIH may give us insight into the pathogenic mechanism of hepatocellular damage in AIH. METHODS: Peripheral blood mononuclear cells (PBMC) were taken from the patients with AIH (34 cases) and healthy controls (25 cases). Liver infiltrating mononuclear cells (LIMCs) were taken from the patients with AIH (18 cases), the patient with chronic hepatitis C (CH-C) (13 cases) and the patients with fatty liver (2 cases). Using flow cytometry, the cells were analyzed for the expression of costimulatory molecules, such as CD80, CD86, and CD152 (CTLA-4). The results were compared with clinical data such as the level of gammaglobulin, histological grade, presence or absence of corticosteroids administration and the response to corticosteroids. RESULTS: The levels of CD80 , CD86 and CD152 PBMC were significantly reduced in the patients with AIH as compared with healthy controls. By contrast, those cells were significantly higher in LIMC than in PBMC of the patients with AIH. Especially, the level of CD86 LIMC showed a marked increase irrespective of the degree of disease activity in the patients with AIH, although CD86 cells were rarely present in PBMC. The levels of CD86 cells were present in significantly higher frequency in patients with AIH than in the patients with CH-C. Furthermore, the patients with AIH with high levels of CD86 LIMC showed good responses to corticosteroids, whereas 2 cases of AIH with low levels of CD86 LIMC did not respond well. CONCLUSION: These results suggest that LIMC overexpressing costimulatory molecules such as CD80 and CD86 appears to play a role in the pathogenesis of AIH. Especially, CD86 molecule expressed on the LIMC may be useful for the diagnosis of AIH and for the prediction of the therapeutic effects of corticosteroids on AIH.     

慢性HBV感染患者外周血CD4~+CD25~+调节性T细胞表达及其临床意义

目的探讨CD4+CD25+调节性T细胞与慢性HBV感染后不同临床转归和临床特点的相关性。方法在26例慢性乙型肝炎(CHB)患者、15例无症状HBsAg携带者(ASC)和11例肝炎肝硬化(LC)患者和16例正常对照者,分离外周血单个核细胞(PBMC),采用流式细胞仪检测CD4+CD25+调节性T细胞的表达水平。结果CHB组和ASC组的CD4+CD25+调节性T细胞占CD4+T细胞的百分率分别为4.40±2.76%和4.43±2.10%,均高于正常对照组(2.70±0.97%),差异显著(P0.01);CD4+CD25+调节性T细胞的表达水平与HBVDNA水平无相关性(r=0.018,P0.05);在HBeAg阳性与阴性组患者CD4+CD25+调节性T细胞的表达也无明显的差异(P0.05)。结论慢性HBV感染者外周血CD4+CD25+调节性T细胞水平升高,可能与HBV感染的慢性化有关。     

乙型肝炎患者外周血单个核细胞端粒酶活性的研究

目的 了解乙型肝炎患者外周血单个核细胞（PBMC）端粒酶活性的表达情况。方法 通过扩增端粒重复序列（TRAP）及光度酶联免疫法，分别检测健康人及各类乙型肝炎患者PBMC的端粒酶水平。结果 各组患者PBMC在植物血凝素（PHA）刺激前均有端粒酶活性的表达，以急性型肝炎组最高，重型肝炎组最低，二者差别具有显著性（P〈0．001）。PHA刺激后与刺激前比较各组端粒酶活性均有显著性升高（P〈0．001），刺激后的端粒酶水平以重型肝炎组为最低，与其他三组比较差别具有显著性（P〈0．05）。慢性重型乙型肝炎经胸腺五肽（TP5）治疗后端粒酶活性显著增高（P〈0．05）。结论 HBV急性感染期PBMC的端粒酶水平升高；慢性感染期PBMC的端粒酶水平在体内被抑制。TP5具有免疫调节作用，能使过低的端粒酶水平趋向于正常。     

自身免疫性肝炎患者外周血NKp46+ILC3细胞和Th17细胞变化及其临床意义探讨*

目的 研究自身免疫性肝炎(AIH)患者外周血NKp46⁺ILC3细胞和Th17细胞百分比变化及其临床意义。方法 2016年2月～2020年2月我院收治的AIH患者43例,使用流式细胞仪检测外周血NKp46⁺3型天然淋巴样细胞(ILC3)和Th17细胞百分比,应用受试者工作特征曲线(ROC)下面积(AUC)分析应用NKp46⁺ILC3细胞和Th17细胞百分比判断AIH患者肝组织炎症活动分级的效能。结果 10例临床重度AIH患者NKp46⁺ILC3细胞和Th17细胞百分比分别为(0.2±0.2)%和(1.3±0.4)%,显著低于或高于33例轻中度AIH患者【分别为(0.5±0.2)%和(0.8±0.3)%,P<0.05】;12例肝组织G3～4级AIH患者 NKp46⁺ILC3细胞百分比和Th17细胞百分比分别为(0.3±0.2)%和(1.3±0.4)%,显著低于或高于31例G1～2级AIH患者【分别为(0.5±0.2)%和(0.8±0.3)%,P<0.05】;以外周血NKp46⁺ILC3细胞百分比≤0.3%为截断点,判断AIH患者肝组织炎症重度活动的AUC为0.774(95% CI:0.592～0.957),其敏感度为75.0%,特异度为87.1%;以外周血Th17细胞百分比＞1.1%为截断点,判断AIH患者肝组织炎症重度活动的AUC为0.853(95% CI:0.734～0.973),其敏感度为66.7%,特异度为90.3%。结论 AIH患者外周血NKp46⁺ILC3细胞水平降低,而Th17细胞水平升高。应用这种变化规律可能有助于判断AIH患者肝组织炎症活动分级,对评估AIH患者病情具有一定的临床意义。     

Increase in CD95 (Fas/APO-1)-positive CD4+ and CD8+ T cells in peripheral blood derived from patients with autoimmune hepatitis or chronic hepatitis C with autoimmune phenomena

BACKGROUND: The expressions of CD95 (Fas/APO-1) and Bcl-2 are determinants of apoptosis in normal lymphocytes, and abnormalities in their expressions might contribute to the induction of autoimmunity. In this study, we examined the expressions of CD95 and Bcl-2 on freshly isolated T and B cells from patients with autoimmune hepatitis (AIH) or chronic hepatitis C associated with autoimmune phenomena (CH-C(AI)). METHODS: The CD95 and Bcl-2 expressions within CD4+ T, CD8+ T, and CD19+ B cell subsets were analysed by two-colour flow cytometry. RESULTS: The surface expression of CD95 was significantly high in both the CD4+ T and CD8+ T cell subsets derived from the patients with AIH and those with CH-C(AI), compared with expression in patients with CH-C and normal subjects. The increase in CD95 expression was associated with the phenotypic conversion of naive CD45RO- to primed CD45RO+ CD4+ T cells. Bcl-2 was detected in the vast majority of peripheral T and B cells. There was no significant difference in the percentage of Bcl-2-positive cells in the CD4+ T cell, CD8+ T cell and CD19+ B cell subsets among the patient groups and normal subjects. CONCLUSIONS: These results indicate that an increase in CD4+ T cells expressing CD45RO and CD95 marks an important subset of AIH and CH-C(AI) patients. These expanded CD95+ CD45RO+ primed T cells most likely reflect a continuous antigen-specific or non-specific activation of T lymphocytes, and/or the persistent presence of activated lymphocytes as a consequence of abnormalities in the peripheral deletion of activated lymphocytes. These persistently activated lymphocytes might play a role in the induction of autoimmunity in AIH and CH-C(AI).     

CD150在成人乙型肝炎疫苗无应答者体外外周血单个核细胞中的表达

目的 了解信号转导淋巴细胞激活分子(SLAM)CD150在乙型肝炎(乙肝)疫苗无应答者体外外周血单个核细胞(PBMC)中的表达.方法 对2007年9月至2009年12月曾行乙肝疫苗接种、HBV标志物检测均阴性的202例患者进行标准程序再免疫,再接种后第7～12个月检测抗-HBs效价.根据抗-HBs效价判定无应答者18例(男11例,女7例);选取应答者18例(男9例,女9例)作为对照.采集无应答者与应答者静脉血18 mL,淋巴细胞分离液密度梯度离心法分离PBMC,流式细胞仪测定细胞膜表面分子CD150.采用SAS统计软件包进行t检验以及Spearman 秩相关检验.结果 乙肝疫苗免疫后,在特异性刺激剂rHBsAg作用下,PBMC中CD150的表达无应答者为(39.20±10.66)%,高于应答者的(23.73±12.41)%,差异有统计学意义(t=2.1947,P<0.05);CD3+CD4+细胞中CD150的表达无应答者为(49.64±11.94)%.亦高于应答者的(37.73±11.02)%,差异无统计学意义(t=1.7175,P>0.05).在非特异性刺激剂植物血凝素(PHA)作用下,PBMC中CD150的表达在无应答者为(39.21±7.37)%,高于应答者的(23.18±12.68)%,差异有统计学意义(t=2.2835,P<0.05).在特异性刺激剂rHBsAg作用下,CD150在PBMC及CD3+CD4+细胞中与抗体效价呈负相关(r=-0.726,P<0.05).结论 CD150可能在机体接种乙肝疫苗后的无应答者中发挥一定作用.

Abstract：

Objective To study the expression of signaling lymphocytic activation molecule (SLAM)CD150 in peripheral blood mononuclear cells(PBMCs)isolated from adult non-responders to recombined yeast gene hepatitis B vaccine.Methods A total of 202 cases were recruited.All these subjects had been immunized with recombined yeast gene hepatitis B vaccine for more than one standard scheme in two years(from Sep 2007 to Dec 2009)and remained negative for hepatitis B markers(HBsAg,anti-HBs,HBeAg,anti-HBe and anti-HBc).After recruitment,all 202 subjects received another standard scheme(0,1 and 6 month)revaccination.The blood samples were collected 7 months later after the first injection of revaccination to detect anti-HBs titer.The PBMCs were isolated from 18 adult non-responders(anti-HBs titer<10 mIU/mL)and 18 adult responders(antiHBs titer≥100 mIU/mL).CD150 expression on cell surface was analyzed by flow cytometry.SAS package was used for t test and spearman rank correlation analysis.Results After rHBsAg stimulation,the percentage of PBMCs expressed CD150 was significantly higher in non-responders (39.20%±10.66%)than responders(23.73%±12.41%)(t=2.1947,P<0.05).The same trend was also observed in rHBsAg stimulated C133+CD4+T cells,but the difference was not statistically significant(49.64%±11.94%vs 37.73%±11.02%)(t=1. 7175,P>0.05).After phytohaemagglutinin (PHA)stimulation,the percentage of CD150-positive PBMCs was also significantly higher in non-responders (39.21%±7.37%)than responders(23.18%±12.68%)(t=2.2835,P<0.05).CD150 expressions in both PBMCs and CD3+CD4+T cells were negatively correlated with anti-HBs titer after rHBsAg stimulation (r=-0.726,P<0.05).Conclusion Activation of CD150 may contribute to the non-response to hepatitis B vaccine.     

慢性乙型肝炎患者干扰素疗效与CD28阳性外周血单个核细胞的关系

探讨干扰素疗效与慢性乙肝患者CD2 8阳性外周血单个核细胞 (PBMCs)的关系。分离 32例慢性乙肝患者及 8例正常对照PBMCs,以间接免疫荧光流式细胞技术检测CD2 8+ PBMCs,结果显示慢性乙肝患者在干扰素治疗前和结束时CD2 8+ PBMCs均较正常对照组显著降低 (分别为P <0 0 1及P <0 0 5 ) ;干扰素治疗结束时患者CD2 8+ PBMCs水平显著升高 ,与治疗前相比差异具有极显著意义 (P <0 0 1) ;抗病毒治疗 3个月后有 2 1例患者达到完全或部分应答标准 ,11例无应答 ;产生完全或部分应答组 ,在治疗前、后CD2 8+ PBMCs均相应高于无应答组 ;进一步分析表明 ,治疗前完全应答组显著高于无应答组 ,差异具有显著性意义 (P <0 0 5 ) ,而部分应答组虽高于无应答组 ,但差异无显著性意义 (P >0 0 5 )。研究结果提示慢性乙肝患者CD2 8+ PBMCs水平可能与患者对干扰素的应答有关 ,似可作为干扰素疗效预测的指标。     

慢性丙型肝炎患者外周血单个核细胞丙型肝炎病毒检测及其意义

目的 探讨外周血单个核细胞（ＰＢＭＣｓ）在丙型肝炎病毒（ＨＣＶ）的感染中的作用。方法 对２２例慢性丙型肝炎患者２１例抗－ＨＣＶ（＋）血液管析患者及１２例健康献血员的ＰＢＭＣｓ分别进行ＨＣＶＲＮＡ,ＨＣＶ抗原检测及电镜观察。结果 （１）２２生丙型肝炎肝炎患者ＰＢＭＣｓ中有７７．３％（１７／２２）ＨＣＶＲＮＡ阳性,（２）感染ＨＣＶ的ＰＢＭＣｓ中电镜下发现复制的ＨＣＶ颗粒;（３）ＨＣＶ颗粒阳笥者的血清和     

Changes of lymphocyte subsets in peripheral blood before and after operation of patients with advanced ovarian carcinoma

Summary Comparison was made between lymphocyte subsets in peripheral blood from patients with benign ovarian tumor and those with advanced ovarian carcinoma. In addition, changes of lymphocyte subsets of patients with ovarian carcinoma before and after operation were also examined. The percentage and absolute number of CD3^–/HLA-DR⁺ (B cells) in peripheral blood from patients with advanced ovarian carcinoma were significantly lower than values from patients with benign ovarian tumor, whereas both percentage and absolute number of CD3^–/HLA-DR^– (null cells) cells in patients with advanced ovarian carcinoma were significantly higher. Although there was no significant difference in natural killer (NK) cell subsets (CD57⁺CD16^– and CD57⁺ CD16⁺ cells) between patients with benign ovarian tumor and ovarian carcinoma, the percentage and absolute number of CD57^–/CD16⁺ (highly differentiated NK cells) cells in patients with ovarian carcinoma were significantly higher than those in patients with benign ovarian tumor. Both the absolute number and percentage of CD3⁺/HLA-DR⁺ (activated T cells) cells in ovarian cancer patients with minimal residual tumors after operation were significantly increased, compared to the levels before operation, while the values in the patients with large residual tumors were significantly decreased. In addition, the percentage and absolute number of CD3^–/HLA-DR^– (null cells) cells in the patients with minimal residual tumors were significantly decreased after operation, while values in the patients with large residual tumors remained unchanged before and after operation. The patients with minimal residual tumors after operation were characterized by a significant increase in the percentage of CD57^–CD16⁺ (highly differentiated NK cells) cells. On the other hand, in the patients with large residual tumors no change of the NK cell subsets was observed before and after operation.Abbreviation NK natural killer - FITC fluorescein isothiocyamate - PE phycoerythrin