TLCS法测定排氚片中黄芪甲苷的含量

目的建立测定排氚片中黄芪甲苷含量方法。方法采用薄层扫描法测定黄芪甲苷含量,硅胶G薄层预制板,氯仿-甲醇-水（13：6：2）10℃以下放置过夜的下层溶液为展开剂,测定波长530nm,参比波长700nm。结果黄芪甲苷点样量在0．20～0．701μg之间和吸收度积分值呈良好的线性关系,平均回收率为96．74％,RSD为2．02％。结论薄层扫描法便捷、灵敏、准确,可作为排氚片的质量控制方法。     

复方苦参注射液在食管癌放疗中的临床效果观察

目的观察分析复方苦参注射液在食管癌放疗中应用的疗效及降低急性放疗副反应的情况。方法对我科住院的67例食管癌患者资料进行回顾性分析,病人分为观察组和对照组两组,对照组行单纯放疗,总剂量DT60-66Gy／30-33次／6-7w,2．0Gy／次／d。观察组在放疗的同时,加用复方苦参注射液静脉滴注治疗。结果放疗结束时以食管吞钡X线造影片判断疗效,观察组和对照组的有效率分别为94．3％,81．3％（X^2=1．60,P〉0．05）,差异无统计学意义;观察组生存质量改善率优于对照组（P〈0．01）;观察组放射性食管炎及放射性气管炎发生率低于对照组（P〈0．05）。结论食管癌放疗的同时加用复方苦参注射液治疗,虽不能明显提高疗效,但可以减少急性放疗副反应。改善患者的一般状况。     

舒痛膏的质量控制

目的 建立舒痛膏的质量控制标准。方法 采用薄层色谱法对方中的栀子、乳香、没药、桃仁进行定性鉴别；用薄层扫描法测定该制剂中栀子苷的含量。结果 栀子苷的回归方程为Y=1238．207X 97．791，r=0．9985，在1．01～7．07μg范围内，线性关系良好；平均回收率为97．82％，RSD为2．55％(n=6)。结论 薄层鉴别方法专属性强；含量测定方法简便、准确、重复性好，适于该复方制剂的质量控制。     

产后康口服液质量标准的研究

目的建立产后康口服液质量标准。方法用双波长薄层反射锯齿扫描测定黄芪甲苷含量。采用薄层色谱法鉴别黄芪、当归、益母草。结果平均加样回收率为97．3％,RSD为1．24％(n=6);在1．0—5．0μg范围内线性良好(r=0．9994)。结论本法操作简易、准确、重复性好,能够控制产后康口服液的质量。     

复方岩连片的质量标准研究

目的完善复方岩连片的质量标准，以控制药品质量。方法采用薄层色谱法对重楼进行定性鉴别，采用HPLC测定石吊兰中石吊兰素的含量。结果薄层色谱法加样鉴别重楼斑点亚色清晰，阴性无干扰，HPLC测得石吊兰素的线性范围为0．200～1．000μg，r=0．9998，平均回收率为99．3％，RSD为0．75％（n=6）。结论本方法操作简单，结果准确，能够有效地控制复方岩连片的质量。     

复方苦参注射液联合化疗治疗中晚期恶性肿瘤的疗效观察

目的观察复方苦参注射液联合化疗治疗中晚期恶性肿瘤的疗效及毒副作用。方法将84例中晚期恶性肿瘤患者随机分为治疗组和对照组,每组42例,治疗组在化疗的基础上加用复方苦参注射液静滴：对照组单纯化疗。2个疗程后评价疗效及毒副反应。结果治疗组与对照组相比,在KPS评分提高率（59．5％VS35．7％）、疾病进展率（19．0％vs40．1％）及化疗毒副反应方面均有明显改善（P〈0．05）;治疗组近期有效率为45．2％,对照组为38．1％,二者无显著性差异（P〉0．05）。结论复方苦参注射液联合化疗能明显提高患者的KPS评分,减轻化疗毒副反应,提高生活质量及延缓疾病进展：     

胃舒颗粒薄层鉴别及含量测定

目的：建立胃舒粒质量标准。方法：采用薄层色谱法鉴别大黄,槟榔,枳实,薄层色谱扫描法测定样品中大黄素的含量。结果：大黄中大黄素在0．15－1．20ug(r=0.9964)范围内呈良好的线性关系。平均回收率为90．82％,RSD为1．98％（n=6)。结论：操作简易,斑点明显,分离效果好;含量测定干扰少,稳定性好,本法能很好地控制胃舒颗粒的质量。     

芎菊上清丸质量标准研究

目的为芎菊上清丸提供质量控制方法。方法采用薄层色谱法对制剂中的栀子、黄芩进行了定性鉴别；以薄层扫描荧光法测定了制剂中盐酸小檗碱的含量。结果薄层色谱斑点清晰；盐酸小檗碱在0．06—0．30μg范围内点样量与荧光强度线性关系良好，平均回收率为101．64％，RSD为0．37％(n=5)。结论栀子和黄芩的定性鉴别及盐酸小檗碱的含量测定方法简便可靠、重现性好，可作为制剂的质量控制方法。     

柱前衍生化HPLC法同时测定复方甘草酸苷胶囊中甘氨酸和蛋氨酸的含量

目的建立同时测定复方甘草酸苷胶囊中甘氨酸和蛋氨酸含量的高效液相色谱法。方法采用C18色谱柱（4．6mm×150mm,5μm）,流动相为乙腈-0．01mol·L^-1磷酸溶液（49：51）,流速：1．0mL／min,检测波长：262nm。结果甘氨酸、蛋氨酸浓度分别在10．6～106．0、10．4～104．0μg·mL^-1范围内呈良好的线性关系;平均回收率分别为100．53％和100．66％,RSD分别为1．08％和1．13％（n=9）。结论本法可用于同时测定复方甘草酸苷胶囊中甘氨酸和蛋氨酸的含量,方法简便,结果准确。     

理气定喘丸的质量标准研究

目的建立理气定喘丸的质量控制方法。方法采用薄层色谱法对方中陈皮、知母、当归、紫苏叶进行鉴别。采用高效液相色谱法测定陈皮中橙皮苷的含量。色谱条件：色谱柱：ODS—C18色谱柱;流动相：乙腈-0．05％磷酸（21：79）;流速：1ml／min;检测波长：283nm;柱温：40℃。结果采用薄层色谱法鉴别了方中陈皮、当归、知母、紫苏叶。含量测定橙皮苷在0．1204～1．8072ug范围内线性良好,r=0．9997。平均回收率为101．9％,RSD为1．7％（n=6）。结论本方法重复性好、简便易行,可作为理气定喘丸的质量控制方法。     

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version

The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

Do Balance Board Training Programs Reduce the Risk of Ankle Sprains in Athletes?

Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).     

High Intensity Interval Training:New Insights

KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief，intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.，≥90%of VO2 peak).     

Endovascular management of carotid-cavernous fistulas

Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas （CCF） and their complications such as pseudoaneurysms. Methods： Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% （4/22）. A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% （8/22）. Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents.     

Developmental validation of the PowerPlex(®) ESI 16 and PowerPlex(®) ESI 17 Systems: STR multiplexes for the new European standard

In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega^® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex^® ESI 16 (European Standard Investigator 16) and the PowerPlex^® ESI 17 Systems. The PowerPlex^® ESI 16 System combines the 11 loci compatible with the UK National DNA Database^®, contained within the AmpFlSTR^® SGM Plus^® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR^® SGM Plus^® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex^® ESI 17 System amplifies the same loci as the PowerPlex^® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR^® SGM Plus^® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex^® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors.     

Bronchial stump fistula :treatment with covered retrievable hinged metallic stents-preliminary clinical experience

Objective To evaluate the preliminaily clinical efficacy and retrievability of a retrievable hinged covered metallic stent in the treatment of the bronchial stump fistula （BSF）. Methods Between April 2003 and March 2005, 8 patients with bronchial stump fistula after pneumonectomy or lobectomy were treated with two types （A and B） of retrievable hinged covered metallic stents. Type A stent was placed in 6 patients and type B in 2 under fluoroscopic guidance. The stent was removed with a retrieval set when BSF was healed or complications occurred. Results Stent placement in the bronchial tree was technically successful in all patients, without procedure-related complications. Immediate closure of the BSF was achieved in all patients after the procedure. Stents were removed from all patients but one. Removal of the stents was difficult in two patients due to tissue hyperplasia. Patients were followed up for 6 - 21 months. Placement of the stents remained stable in all patients except one due to severe cough. Permanent closure of BSF was achieved in 7 （87.5%） of 8 patients. Conclusion Use of a retrievable hinged covered expandable metallic stent is a simple, safe, and effective procedure for closure of the BSF. Retrieval of the stent seems to be feasible. （J Intervent Radiol, 2007, 16： 253-257）     

Prevalence and severity of hip and groin pain in sub‐elite male football: a cross‐sectional cohort study of 695 players

The purpose of this study was twofold: (a) to investigate the prevalence of hip and groin pain in sub‐elite male adult football in Denmark and (b) to explore the association between prevalence and duration of hip and groin pain in the previous season with the Copenhagen Hip and Groin Outcome Score (HAGOS) in the beginning of the new season. In total 695 respondents from 40 teams (Division 1–4) were included. Players completed in the beginning of the new season (July–Sept 2011) a self‐reported paper questionnaire on hip and/or groin pain during the previous season and HAGOS. In total 49% (95% CI: 45–52%) reported hip and/or groin pain during the previous season. Of these, 31% (95% CI: 26–36%) reported pain for >6 weeks. Players with the longest duration of pain during the previous season had the lowest HAGOS scores, when assessed at the beginning of the new season, P < 0.001. This study documents that half of sub‐elite male adult football players report pain in the hip and/or groin during a football season. The football players with the longest duration of pain in previous season displayed the lowest HAGOS scores in the beginning of the new season.