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1.
TIG2基因在肺癌细胞中表达及启动子区甲基化的研究   总被引:1,自引:0,他引:1  
 目的 探讨TIG2基因在原发性非小细胞肺癌中的表达及意义.方法 应用RT-PCR法检测TIG2基因在肺癌和正常肺细胞中表达变化,用CpG岛预测软件分析TIG2基因启动子和第一外显子区域CpG岛分布,用甲基化特异PCR法(MSP)检测TIG2基因甲基化.用RT-PCR法检测去甲基化试剂作用后TIG2基因的表达.结果 正常肺细胞和癌旁正常组织中TIG2mRNA呈高表达,而肺癌细胞系和组织中低表达或沉默.TIG2基因的启动子和(或)第一外显子区域存在典型的CpG岛.在肺癌细胞系均发生TIG2甲基化,肺癌组织中常发生甲基化,而肺癌旁组织中未见甲基化.去甲基化试剂作用后TIG2基因恢复表达.5-氮-2'-脱氧胞苷浓度越高,TIG2mRNA表达的强度越大.结论 甲基化是导致TIG2基因转录沉默的重要机制,TIG2可能是一种新的肺癌肿瘤抑制基因.  相似文献   

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目的多重耐药(drug resistance,MDR)是肿瘤化疗失败的主要原因。本研究旨在探讨MDR1甲基化对宫颈癌经皮子宫动脉血管腔内化疗栓塞疗效的影响。 方法选择67例宫颈癌患者接受栓塞化疗,45例正常宫颈组织作为对照。免疫组化检测宫颈癌中P-糖蛋白(P-gp)水平,并与正常组织进行比较。通过使用特异性千碱基裂解法和基质辅助激光解吸电离飞行时间质谱法分析MDR1基因启动子区16个CpG岛的甲基化状态。 结果P-gp在正常宫颈组织中的阳性表达率为0% (0/45),介入栓塞化疗前后的阳性表达率分别为61.19%(41/67)和77.61%(52/67)。宫颈癌组织与正常宫颈组织相比有明显差异(χ2=4.2523,0.0392)。化疗前P-gp的阳性表达率与化疗疗效呈负相关(r=-0.340,P=0.005)。正常组织中13个CpG岛的甲基化率显著高于宫颈组织(P<0.05)。在宫颈癌组织中,经皮子宫动脉血管腔内化疗栓塞前6个CpG岛的甲基化率高于化疗后,但1个CpG岛的甲基化率低于化疗后(P<0.05)。化疗前有效化疗的1个CpG岛甲基化率明显高于无效化疗(P<0.05),其他CpG岛相似(P<0.05)。 结论MDR1编码的P-gp表达水平,部分MDR1基因启动子区CpG岛的甲基化状态,与宫颈癌介入栓塞化疗的疗效密切相关。  相似文献   

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目的探讨人卵巢癌组织差异表达基因,揭示卵巢癌发生、发展的分子机制。方法人卵巢癌组织标本2例,均经病理确诊为低分化浆液性乳头状囊腺癌,临床诊断为卵巢癌Ⅲ期,选用2例正常卵巢组织为对照。提取卵巢组织中mRNA,提取并纯化卵巢癌组织中总RNA,应用cDNA基因芯片技术对总共588个基因进行差异表达基因检测。结果共筛选出差异表达基因44条,其中11条表达上调(包括癌基因c-erbB2、neu、c-fos、c-mycproto-oncogenes、HER2receptor等)、33条表达下调(包括RAR、MMP18、MMP19、p21、DNA-PK等)。结论揭示了人卵巢癌组织中差异表达基因,有望为早期诊断卵巢癌及判断预后提供理想标志物。  相似文献   

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目的:建立ASPP1甲基化状况的检测方法,研究ASPP1基因启动子在HEPG-2肝癌细胞株和成纤维细胞株中的甲基化状况。方法:利用因特网对ASPP1基因启动子的CpG岛进行分析,并设计MSP引物,然后对正常的成纤维细胞和p53野生型肿瘤细胞株HEPG-2的ASPP1 DNA启动子甲基化状况进行检测。结果:MSP分析显示,在正常的成纤维细胞中,ASPP1基因启动子CpG岛未出现甲基化;在p53野生型肿瘤细胞株HEPG-2中,ASPP1基因启动子CpG岛处于甲基化状态。结论:MSP检测ASPP1启动子CpG岛甲基化状况的方法客观、准确、可靠。  相似文献   

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目的:检测上皮性卵巢癌组织中WT1蛋白的表达,探讨其临床病理学意义及在上皮性卵巢癌发生、发展中的作用。方法:采用免疫组化SP法检测正常卵巢组织20例及卵巢良性上皮性肿瘤20例及上皮性卵巢癌20例,上皮性卵巢癌40例标本中WT1蛋白的表达情况。结果:上皮性卵巢癌组织中WT1阳性表达率为57.5%,显著高于正常卵巢组织及卵巢良性上皮性肿瘤(P〈O.01);卵巢交界性上皮性肿瘤组织中WT1阳性表达率为45.0%,也显著高于正常卵巢组织及卵巢良性上皮性肿瘤(P〈O.01);在上皮性卵巢癌中,浆液性囊腺癌WT1蛋白的阳性表达率(90.9%),显著高于其他亚型(P〈0.01)。结论:WT1可能成为卵巢上皮性肿瘤恶性转化和上皮性卵巢癌鉴别诊断的客观辅助指标,并为其诊断及预后判断提供重要参考。  相似文献   

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王莉  惠宁  满晓波  唐亮 《解放军医学杂志》2007,32(10):1017-1019
目的 探讨GPC3在卵巢癌中的表达及其临床意义.方法 利用实时荧光定量RT-PCR技术检测2004年1月~2005年10月手术获得的35例卵巢上皮性癌组织及23例正常卵巢组织中GPC3 mRNA的表达量,并结合卵巢癌的临床病理特点进行分析.利用Western blot技术检测4例卵巢癌组织和3例正常卵巢组织中GPC3蛋白的表达水平.结果 GPC3 mRNA在卵巢癌组织中的表达量低于正常卵巢组织(P<0.05),部分卵巢癌组织无GPC3 mRNA表达.GPC3 mRNA的表达量与卵巢癌临床分期有关,在Ⅲ、Ⅳ期卵巢癌组织中的表达量显著低于Ⅰ、Ⅱ期(P<0.05).GPC3 mRNA与CA125在卵巢癌中的表达无相关性(P>0.05).GPC3蛋白在正常卵巢组织中的表达水平是卵巢癌组织中的2~3倍.结论 GPC3在卵巢癌的发生发展中起着一定作用,其与CA125联合应用有可能提高卵巢癌的阳性诊断率.  相似文献   

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夏光  吕庆杰  孙德飞  姜卫国 《临床军医杂志》2009,37(5):836-838,F0004
目的研究Wnt/β-catenin信号通路的枢纽分子β-连环素(β-catenin)及其下游靶基因c-Myc在卵巢癌中的表达。方法应用免疫组化S-P法检测60例卵巢癌、24例卵巢上皮良性肿瘤以及26例正常卵巢上皮组织中β-catenin和c-Myc的表达情况。结果β-catenin在卵巢癌组织的异位表达与正常卵巢组织相比,差异有统计学意义(P<0.05);β-catenin与卵巢癌的组织学分型以及分化程度具有相关性(P<0.05);c-Myc在卵巢上皮癌中表达分别与卵巢上皮良性肿瘤和正常卵巢上皮组织组相比,差异有统计学意义(P<0.05);c-Myc与卵巢癌分化程度相关(P<0.05);在卵巢癌组织中,β-catenin的异位表达与c-Myc的表达具有相关性(P<0.05)。结论Wnt/β-catenin信号通路可能是卵巢癌的发生途径。  相似文献   

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卵巢癌组织中GST—π和MDR1基因的表达及其意义   总被引:2,自引:0,他引:2  
目的:观察两种耐药基因谷胱甘肽S-转移酶-pi(GST-π)和MDR1在卵巢癌组织中mRNA水平的表达情况,探讨其表达的意义及应用价值,方法:采用逆转录PCR方法检测了18例卵巢癌和10例正常卵巢组织中GST-π和MDR1的表达,应用β-肌动蛋白作为内对照进行定量分析比较,结果:GST-π和MDR1在正常卵巢组织中表达的阳性率分别为20%和10%,在卵巢癌中的阳性表达率分别是61.1%和33.3%,GST-π在癌组织表达高于正常组织,两者比较P<0.05,而DR1在癌组织表达虽高于正常组织但无统计学意义,在癌组织中有4例同时出现了GST-π和MDR1的共建表达均高于正常卵巢组织,但以GST-π为主,(2)GST-π和MDR1在癌组织中的表达不一致,说明两者的作用机制不同,而共表达现象的存在,又说明GST-π和MDR1可能有相的同调节因素存在,(3)GST-πmRNA水平的表达低于蛋白水平的表达,提示GST-π表达达存在转录后加工和(或)翻译水平的调控。  相似文献   

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测定卵巢组织标本中抗原的含量.方法:对33份卵巢组织标本用放射性碘标记单克隆抗体MoV18进行定量放射自显影测定.其中22份为卵巢癌,7份为卵巢良性肿瘤,4份为正常卵巢组织.结果:19份浆液性卵巢癌中,17份有CA-MoV18抗原表达,含量为1.30~59.28pmol/g组织.3份粘液性卵巢癌及11份非恶性卵巢组织中(7份良性病变,4份正常组织)未测得CA-MoV18抗原.结论:CA-MoV18抗原在浆液性卵巢癌中有表达,其含量与肿瘤组织中标记抗体含量(%ID/g)有关.关键词  相似文献   

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高同型半胱氨酸血症致血管平滑肌细胞增殖的机制初探   总被引:1,自引:0,他引:1  
 目的 探讨高同型半胱氨酸血症(hyperhomocysteinemia, HHcy)致血管平滑肌细胞(vascular smooth muscle cells, VSMC)增殖在动脉粥样硬化发病中的机制.方法 整体水平实验:82例研究对象分为动脉粥样硬化(atherosclerosis,AS)组54例,对照组28例.荧光生化法检测血浆总同型半胱氨酸(total homocysteine, tHcy)水平,甲基化特异的 PCR(Methy-specific PCR,MSP)法检测雌激素受体α(estrogen receptor-α ,ER-α)启动子区CpG岛的甲基化状态.细胞水平实验:体外培养的人脐动脉VSMC,用不同浓度同型半胱氨酸(homocy steioe, Hcy)处理,MTT法观察Hcy对平滑肌细胞生长的影响,MS-PCR法和Rt-PCR法检测ER-α基因启动子区甲基化程度及mRNA的表达.结果 整体水平实验中,AS患者中甲基化率为70.4%,对照组甲基化率为28.6%,差异具有统计学意义(P<0.05).细胞水平实验中,MTT结果显示Hcy对VSMC具有明显促增殖效应.Hcy显著增加ER-α基因启动区的甲基化修饰,并使ER-αmRNA的表达进行性减少.结论 Hcy通过干扰DNA的甲基化修饰,使ER-а基因高甲基化可能是高同型半胱氨酸血症促使VSMC增殖,并引起动脉粥样硬化的重要机制.  相似文献   

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The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.  相似文献   

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Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas (CCF) and their complications such as pseudoaneurysms. Methods: Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% (4/22). A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% (8/22). Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents.  相似文献   

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Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.  相似文献   

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Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).  相似文献   

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KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief,intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.,≥90%of VO2 peak).  相似文献   

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In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex® ESI 16 (European Standard Investigator 16) and the PowerPlex® ESI 17 Systems. The PowerPlex® ESI 16 System combines the 11 loci compatible with the UK National DNA Database®, contained within the AmpFlSTR® SGM Plus® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR® SGM Plus® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex® ESI 17 System amplifies the same loci as the PowerPlex® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR® SGM Plus® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors.  相似文献   

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