伤害性刺激对躯体感觉皮层单位放电的影响及电针效应

<正> 大脑皮层在针刺镇痛中的作用是研究针刺镇痛原理的课题之一,以往我们曾在正常人、颅脑手术病人以及实验动物上,用大脑皮层诱发电为指标研究机体对痛刺激(或伤害性刺激)时的大脑皮层诱发电位,并观察某些镇痛药及针刺的影响。     

电针不同经穴对不同性周期雌鼠黑质神经元放电的影响

探讨不同经穴的镇痛作用及针刺镇痛中多巴胺（ＤＡ）与性激素的关系。方法在去卵巢（ＯＶＸ）大鼠和ＯＶＸ加注雌二醇（ＯＶＸ＋Ｅ２）大鼠黑质（ＳＮ）神经元的自发放电和几种刺激的诱发反应之后,分别电针“肾俞”或“三阴交”两穴,以观察电针不同经、穴对上述反应的影响。结果电针“三阴交”后,两组自发放电均趋抑制;而电针“肾俞”后,两组放电均有不同程度激活。结论经、穴对同一ＳＮ单位的不同作用表明经、穴是相对独立存在的,各经、穴分别产生不同的调节机制;针刺在镇痛过程中可能对体内的激素水平、镇痛系统和递质系统产生了复杂的综合调节作用。     

电针对帕金森病模型大鼠黑质多巴胺能神经元凋亡的影响

目的 观察电针对帕金森病大鼠黑质DA神经元凋亡的影响.方法 将6-OHDA注入中脑右侧黑质造成单侧黑质损毁的帕金森病大鼠模型,采用电针对模型大鼠进行治疗,并用TH和TUNEL双标法检测正常组、假手术组、模型组、电针组黑质DA神经元凋亡数目.结果 帕金森病大鼠损毁侧DA神经元凋亡数目显著高于正常组（P＜0.01）及未损毁侧（P＜0.01）,电针可有效地减少帕金森病大鼠DA神经元凋亡数目（P＜0.05）.结论 DA神经元凋亡可能参与帕金森病的发生,电针可有效地改善这一病理过程.     

电针风府穴、太冲穴对PD大鼠黑质神经元凋亡的影响

目的 :观察PD大鼠黑质神经元凋亡的变化及电针的影响。方法 :采用 6 -OHAD注入毁损单侧黑质造成PD大鼠模型 ,并用TUNEL法标记凋亡神经元 ,用电镜观察神经元超微结构的变化。结果 :PD大鼠毁损侧黑质凋亡神经元数量显著高于正常组 (P <0 0 1) ,电镜下可见细胞收缩 ,核染色质浓缩等超微病理改变。电针组毁损侧黑质凋亡神经元数量低于模型组 (P <0 0 5 ) ,超微病理改变不如模型明显。结论 :细胞凋亡是PD黑质多巴胺能神经元变性死亡的结果 ,电针可有效地缓解这一病理过程。     

电针对帕金森病大鼠中脑黑质多巴胺能神经元氧化应激损伤的影响

目的:观察电针对帕金森病(PD)大鼠的中脑黑质超氧化物歧化酶(SOD)活力和谷胱甘肽(GSH)、谷胱甘肽过氧化酶(GSH-Px)、丙二醛(MDA)含量及对中脑黑质酪氨酸羟化酶(TH)表达及多巴胺(DA)能神经细胞凋亡的影响,探讨电针治疗PD的可能机制。方法:雄性Wistar大鼠随机分为正常组(n=10)、模型组(n=11)、电针组(n=11)和西药组(n=11)。皮下注射鱼藤酮溶液制备PD大鼠模型。电针组选"百会""三阴交""太冲"穴电针10min,每天1次,7d为1个疗程,治疗2个疗程;西药组给予美多巴混悬液灌胃(1.67mg/kg)。治疗后行酶法测定右脑中脑黑质SOD、GSH-Px活力,比色定量分析法测定GSH、MDA含量;免疫组化(SP法)染色检测左脑中脑黑质TH,TUNEL法检测DA能神经细胞凋亡。结果:模型组SOD、GSH、GSH-Px均低于正常组(P0.05),模型组MDA高于正常组(P0.05);电针组SOD、GSH、GSH-Px均高于模型组(P0.05),电针组、西药组MDA均低于模型组(P0.05);电针组SOD、GSH-Px均高于西药组(P0.05)。电针组、西药组与模型组相比中脑黑质DA能神经元细胞形态较规则,体积较正常,核仁较明显,神经纤维排列较整齐,结构较密集;西药组改善差于电针组,优于模型组。电针组、西药组与模型组相比TH改善明显,阳性神经元数目增多(P0.05),神经元胞体轮廓及突起清晰;西药组改善差于电针组(P0.05)。电针组、西药组与模型组相比,DA能神经细胞凋亡减少(P0.05)。结论:电针可通过提高PD大鼠中脑黑质SOD、GSH-Px活力和GSH含量,降低MDA含量,增加TH免疫反应阳性神经元数目,抑制DA能神经细胞凋亡来治疗PD。     

伤害性刺激对大鼠丘脑腹后外侧核痛兴奋神经元和痛抑制神经元放电的影响及电针对其调制作用

<正> 研究指出,丘脑腹后外侧核(VPL)可接受躯体伤害性传入信息,在VPL中可记录到对伤害性刺激出现放电增加和放电减少的痛反应神经元。表明VPL某些神经元对伤害性刺激的反应与丘脑非特异投射系统核团中痛反应神经元的活动相似     

电针对帕金森病大鼠黑质细胞形态及凋亡的影响

目的:观察针刺对帕金森病(PD)大鼠黑质多巴胺(DA)能神经元的形态数量及黑质细胞凋亡率的影响,探讨电针治疗PD的部分作用机制。方法:Wistar大鼠40只随机分为正常组、假手术组、模型组和电针组,以6-羟基多巴胺右侧纹状体微量注射法制备PD大鼠旋转模型。电针组取"风府""太冲"电针治疗,每日1次,共治疗2周。采用HE染色观察各组黑质的形态学变化,以Nissl染色检测各组黑质的神经元总数,以免疫组织化学法检测各组黑质、纹状体酪氨酸羟化酶(TH)阳性细胞和纤维的数量,采用流式细胞术检测各组黑质TH阳性细胞百分比及细胞凋亡比例。结果:模型组损毁侧黑质致密部神经元总数、TH阳性神经元数量及纹状体TH阳性神经纤维数量显著低于正常组和假手术组(P0.01),电针组与模型组比较各相应指标数值均显著增加(P0.01);电针组损毁侧黑质细胞凋亡率显著低于模型组(P0.01),但仍较正常组和假手术组显著增高(P0.01)。结论:电针治疗能显著提高PD模型大鼠黑质DA能神经元数量及纹状体DA能神经纤维密度,显著降低PD模型鼠黑质细胞凋亡率,说明电针干预对PD模型鼠黑质纹状体系统多巴胺能神经元具有明显的保护作用。     

电针帕金森模型大鼠对黑质TH含量的影响

目的 :观察电针对帕金森病大鼠黑质TH含量的影响。方法 :将 6 OHDA注入黑质造成单侧黑质损毁的帕金森病大鼠模型 ,并用免疫组织化学法检测正常组、假手术组、模型组、电针组黑质TH阳性神经元数目。结果 :帕金森病大鼠损毁侧SNC、SNL的TH阳性神经元数目显著低于正常组 (P <0 .0 1 )及未损毁侧 (P <0 .0 1 ) ,电针可有效地增高帕金森大鼠损毁侧SNC、SNL的TH阳性神经元数目 (P <0 .0 1 ,P <0 .0 5)。结论 :帕金森病大鼠黑质多巴胺能神经元合成多巴胺的功能低下 ,电针可有效地改善这一病理状态     

电针刺激穴位对下腹部皮肤伤害性刺激引起的大脑皮层诱发电位的影响

本工作试图以下腹部皮肤伤害性刺激引起的大脑皮层诱发电位(EP)为指标,探讨电针刺激穴位与手术切口部位之间在神经中枢是否存在相对的特异性联系,同时为临床寻找更为有效的穴位和适宜的电针刺激参数。现将25只兔的实验结果介绍如下:1.用频率为15赫的电针刺激腰1穴(新部位)或“次髎”穴时,对EP的压抑作用最为明显;“足三里”和“三阴交”穴的作用次之;“曲池”穴和“四白”穴无何影响。实验结果说明,穴位对下腹部皮肤伤害性刺激所引起的大脑皮层EP的影响,在空间上具有相对的特异作用,其机理可能与神经的节段性支配有关。2.在刺激强度不变的条件下,用频率为5、15、40和80赫电针刺激“次髎”穴,结果发现以5和15赫的频率对EP的压抑作用最明显;40赫的作用较差;80赫无何作     

急性关节炎大鼠束旁核神经元放电变化及电针效应

<正> 丘脑束旁核(Pf)在疼痛信息的传递,感觉和整合过程中起重要作用,这已被大量的实验资料所证实,但既往的工作多是在正常动物上进行的。我们曾观察到大鼠在局部注射弗氏完全佐剂诱发急性关节炎后处于痛敏状态,表现为电刺激尾尖引起的嘶叫阈降低,患肢局部辐射热引起的缩肢阈降     

EFFECTS OF ELECTROACUPUNCTURE ON THE NEUROENDOCRINE SYSTEM IN HYPOTHYROID AND HYPOADRENOCORTIC RATS

Endocrinehormonesareinvolvedinregula-tionofsubstancemetabolismandimmunnity,antiinf1ammationandsecretionofotherhor-monesintheorganism.Agreatnumberofstud-iesindicatethatacupuctureisinvolvedintreat-mentofmanykindsofdiseasesthroughregulat--ingendocrinef...ti.…     

EFFECTS OF LOW-FREQUENCY ELECTROACUPUNCTURE ON THE IMMUNOLOGIC FUNCTION IN MORPHINE DEPENDENCE RATS

InGoodmanandGilmans’bookPharmaco logicalBasisofTherapeutics[1 ] ,acupuncturetherapyhasbeenregardedasthethirdtypeofremediesforheroinomania .However,uptonow ,majorityoftheresearchesfocusonhowtotreatwithdrawalsymptoms,feweronthereha bilitationofthepatient’simmunefunction .Inthepresentpaper,theeffectoflow frequencyelectroacupuncture (EA)onthecontentofTlymphocytesubgroupsintheperipheralbloodisobservedforrevealingmechanismsofacupunc tureinraisingimmunologicfunction .1　MATERIALSANDMETHOD…     

EFFECT OF ELECTROACUPUNCTURE ON SYNAPTIC PLASTICITY OF HIPPOCAMPAL NEURONS IN CEREBRAL ISCHEMIA RATS

Objective: To observe the effect of electroacupuncture (EA) on synaptic structure of hippocampal nerve felts and synaptophysin(SYN)expression in rats with cerebral ischemic injury. Methods: Sixty Wistar rats were randomized into sham-operation group, cerebral ischemia (CI) group and EA group, each of which was further divided into 1week (W) and 5W subgroups. Cl injury model was established by occlusion of the bilateral common carotid arteries. "Baihui"(百会 GV 20), "Dazhui" (大椎 GV 14), "Renzhong"(人中 GV26) and "Guanyuan"(关元 CV 4) were punctured and stimulated electrically. The brain tissue sections containing hippocampus region were stained with immu nohistochemical technique and observed under light microscope and transmission electronic microscope. Results: After CI, the ischemic injury as degeneration of the presynapse compositions, decrease of the synaptic numeral density, and low expression of SYN were observed in hippocampal CA1 area. By the 5th week after CI, the neonatal synapses of CI and     

家兔黑质内注射多巴胺对电针镇痛作用的影响

<正> 脑内多巴胺能神经元的细胞体主要分布在黑质致密部,由此发出黑质纹体束进入同侧尾核、壳核和杏仁核。尾核内多巴胺(DA)浓度增高时,可能有对抗电针镇痛的作用。本文向黑质内微量注射DA,观察当黑质DA能神经元本身的胞体和树突受到高浓度DA的作用时,对电针镇痛作用的影响。方法实验动物为白色家兔,体重2公斤左右,雌雄不拘。实验前,在氨基甲酸乙酯(0.75克/公斤)轻麻下,按Sawyer氏图谱,向两侧黑质埋植不锈钢管(P_5、H_(-5)、L、R_4)。套     

EFFECT OF ELECTROACUPUNCTURE ON DISCHARGES OF PAINREACTION NEURONS IN CAUDATE NUCLEUS AND PARAFACICULAR NUCLEUS IN RATS WITH CEREBRAL HEMORRHAGE

The bidirectional adjustment of acupuncture means that when the physiological activities of the human body is undergoing an i mbalance state , acupuncture sti mulation of some acupoints may facili- tate the transformation of this abnormal state or nor- malize the body’s physiological performance to recov- er its original equilibriumor establish a newequilibri- um[1]. The electrical activity of neurons is one of the i mportant indexes reflectingthefunctional state of the central nervous system.…     

STUDY ON PROTECTIVE ACTION OF ELECTROACUPUNCTURE ON INJURY OF NEURONS IN RATS OF FOCAL ISCHEMIA BY MICROSCOPY AND ELECTRON MICROSCOPY

Cerebrovasculardiseaseisoneofthefirstsixseverediseasesindeathcausesinourcoun-try.Amongcerebrovasculardiseases,morbidityofischemicdiseaseisthehighest.Ischemicin-juryofneuronsoncereachestheirreversiblestage,regenerationandrenovationofneuronsaredifficult.Thu…     

NEURONS DISCHARGE AND C-FOS EXPRESSION IN NTS FOLLOWING ELECTROACUPUNCTURE AT ACUPOINTS OF STOMACH MERIDIAN OF FOOTYANGMING

The nucleus of solitary tract(NTS) is a primary center for both visceral afferents and somatic afferents.Previous experiments demonstrated that NTS is associated with the connection of the stomach and acupoints in Stomach Meridian of Foot-Yangming(ST Meridian).In this study, extracellular recording and immunochemistry method were used to analyze the discharging neurons and c-Fos protein expression in NTS after electroacupuncture at different acupoints and non-acupoint.A total of 104 discharging neurons were detected in the NTS of 52 rats, of which, 86 were provided with complete data.After acupuncturing at Sibai(ST2), Zusanli(ST36), Neiting(ST44), Quanliao(SI18) and non-acupoint, the response neuron occurring rates(RNORs) in NTS were 65.12%, 51.16%, 46.51%, 34.88% and 31.40% respectively.For RNORs, there was a significant difference among Sibai(ST2), Zusanli(ST36), Neiting(ST44), Quanliao(SI18) and nonacupoint(P0.01 or P0.05).Accordingly, in other 48 rats, the number of c-fos immunoreactive(c-fos-IR) neurons in the NTS after electroacupuncture(EA) at Sibai(ST2) was significantly higher than that of at other acupoints and non-acupoint(P0.05 or P0.01).Both EA at Zusanli(ST36) and Neiting(ST44) increased c-fos-IR neurons significantly compared with that of at Quanliao(SI18) and non-acupoint(P0.05 or P0.01), while there was no difference between EA at Quanliao(SI18) and nonacupoint(P0.05).The experiments demonstrated that the afferent convergence in NTS is different by body surface point stimulus, which suggested that NTS might be a primary center in the central nervous system(CNS) which received acupoint stimulus from ST Meridian.     

不同频率对关节炎大鼠痛反应及中枢γ—氨基丁酸和谷氨酸含量的影响

电针在急性关节炎动物模型上有镇痛作用,但不同电针参数的研究未见报道。本实验用100赫芝和15赫芝两种频率电针关节炎大鼠阳陵泉穴,可见低频电针镇痛效应优于高频电针;低频电针组脊髓谷氨酸含量低于高频电针组,γ-氨基丁酸含量在两组间无明显著差别。结果提示脊髓谷氨酸可能与不同频率电针的镇痛效应有密切关系。