老鹰茶正丁醇部分的化学成分

目的对樟科植物老鹰茶叶醇提取物中正丁醇部分的化学成分进行研究。方法老鹰茶经70%乙醇提取,再经石油醚、氯仿、乙酸乙酯和正丁醇依次萃取,正丁醇部分用大孔树脂、硅胶、Sephadex LH-20以及制备高效液相色谱法等进行分离纯化,通过1H-NMR、13C-NMR、MS等方法鉴定化合物的结构。结果从老鹰茶叶的正丁醇部分分离得到7个化合物,分别鉴定为原儿茶酸、山奈酚-3-O-β-D-(6-O-trans-p-香豆酰基)吡喃葡萄糖苷、山奈酚-3-O-β-D-(6-O-trans-p-香豆酰基)吡喃甘露糖苷、山奈酚-3-O-α-L-鼠李糖苷、反式香豆酸、邻苯二甲酸二正丁酯、水杨酸。结论这7种化合物均为首次从该植物中分离得到。     

Chemical constituents of n-Butanol fraction of Litsea coreana L.

目的 对樟科植物老鹰茶叶醇提取物中正丁醇部分的化学成分进行研究.方法 老鹰茶经70%乙醇提取,再经石油醚、氯仿、乙酸乙酯和正丁醇依次萃取,正丁醇部分用大孔树脂、硅胶、Sephadex LH-20以及制备高效液相色谱法等进行分离纯化,通过1H-NMR、13C-NMR、MS等方法鉴定化合物的结构.结果 从老鹰茶叶的正丁醇部分分离得到7个化合物,分别鉴定为原儿茶酸、山奈酚-3-O-β-D-(6-O-trans-p-香豆酰基) 吡喃葡萄糖苷、山奈酚-3-O-β-D-(6-O-trans-p-香豆酰基) 吡喃甘露糖苷、山奈酚-3-O-α-L-鼠李糖苷、反式香豆酸、邻苯二甲酸二正丁酯、水杨酸.结论 这7种化合物均为首次从该植物中分离得到.     

红凤菜化学成分研究

目的 研究菊科三七草属植物红凤菜Gynura bicolor DC.地上部分的化学成分.方法 采用硅胶、凝胶柱色谱等方法 分离化合物,运用理化性质和现代光谱技术鉴定化合物的结构.结果 分离得到17个化合物,分别鉴定为:十八碳脂肪醇(Ⅰ)、十一碳脂肪酸(Ⅱ)、二十六碳脂肪酸(Ⅲ)、三十碳脂肪酸(Ⅳ)、己烷(Ⅴ)、对羟基苯甲酸(Ⅵ)、山柰酚(Ⅶ)、山柰酚-3-O-β-D-葡萄糖苷(Ⅷ)、高车前苷(Ⅸ)、山柰酚-3-O-β-D-吡喃葡萄糖基(6→1)-α-L-鼠李糖苷(Ⅹ)、槲皮素-3-O-β-D-吡喃葡萄糖基(6→1)-α-L-鼠李糖苷(Ⅺ)、槲皮素-双-3-O-β-葡萄糖苷(Ⅻ)、β-香树脂醇(Ⅻ)、α-香树脂醇(ⅩⅣ)、β-香树脂醇-3-O-β-葡萄糖苷(ⅩⅤ)、乙酰表木栓醇(ⅩⅥ)、β-谷甾醇(Ⅶ).结论 全部17个化合物均为首次从红凤菜中分离得到.     

山栀茶化学成分的研究

目的:对山栀茶的化学成分进行研究.方法:应用正、反相硅胶和Sephadex LH-20等柱层析方法分离纯化山栀茶,根据化合物的理化性质和波谱数据进行结构鉴定.结果:从山栀茶中分离得到7个化合物,分别鉴定为(1)丁香脂素4,4'-双-O-β-D-葡萄糖苷、(2)紫丁香苷、(3)3,4,5-三甲氧基苯-1-O-β-D-呋喃芹糖-(1→6)-β-D-吡喃葡萄糖苷、(4)丁香脂素-4-O-β-D-吡喃葡萄糖苷、(5)异落叶松树脂醇-9'-O-β-D-吡喃葡萄糖苷、(6)南烛木树脂酚-9'-O-β-D-吡喃葡萄糖苷、(7)芥子醛葡萄糖苷.结论:化合物(2),(4),(5),(7)为首次从该植物中分离得到,为山栀茶质量控制指标性成分的选择提供了依据.     

云南蕊木枝叶化学成分研究

目的研究云南蕊木枝叶的化学成分。方法采用柱色谱及薄层色谱进行分离纯化,根据其理化性质及波谱数据鉴定化合物结构。结果分离得到13个化合物,分别为11,12-de(methylenedioxy)danuphyllin(Ⅰ)、蕊木碱甲(methyl chanofruticosinate,Ⅱ)、蕊木碱丙(methyl11,12-methylenedioxychanofruticosinate,Ⅲ)、蕊木碱乙(methyl demethoxycarbonylchanofruticosinate,Ⅳ)、bauerenyl acetate(Ⅴ)、高根二醇(erthrodiol,Ⅵ)、β-香树脂醇(β-amyrin,Ⅶ)、β-香树脂醇乙酸乙酯(β-amyrin acetate,Ⅷ)、羽扇豆醇(lupeol,Ⅸ)、β-谷甾醇(β-sitosterol,Ⅹ)、β-豆甾醇(β-sitosterol,Ⅺ)、myrtifolic acid(ⅩⅡ)、β-谷甾醇-3-O-β-D-葡萄糖苷(β-sitosterol-3-O-β-D-glucoside,ⅩⅢ)。结论化合物Ⅰ～Ⅳ为吲哚生物碱,化合物Ⅴ～ⅩⅢ为首次从该种植物中得到。     

小叶爬崖香中苷类化合物的研究

为探究胡椒属植物小叶爬崖香（Piper sintenense Hatusima）中苷类化学成分,采用硅胶、ODS、MCI GEL CHP20P、Sephadex LH-20柱色谱和半制备高效液相等色谱技术,从小叶爬崖香95%乙醇提取物的正丁醇部位分离获得9个苷类化合物。根据理化性质及核磁共振数据,以上化合物被鉴定为(2S)-2-羟基-1-(4-羟基-3-甲氧基苯基)-1-丙酮-2-O-β-D-葡萄糖苷(1)、苯乙醇-β-D-葡萄糖苷(2)、苄醇-β-巢菜糖苷(3)、苄醇-β-樱草糖苷(4)、苯乙醇-β-D-呋喃芹菜糖基-(1’’→2’)-β-D-葡萄糖苷(5)、红景天苷(6)、苯乙醇-β-樱草糖苷(7)、(顺)-3-己烯醇-β-巢菜糖苷(8)、(顺)-3-己烯醇-β-樱草糖苷(9)。其中化合物1为新化合物,化合物3～9为首次从胡椒属中分离得到。     

太白杜鹃的化学成分研究

目的 研究太白杜鹃 Rhododendron purdomii 的化学成分。方法 利用现代波谱技术(MS、1H-NMR、13C-NMR、DEPT)和化学方法进行结构鉴定。结果 从太白杜鹃全草的醋酸乙酯部分中分离出12个化合物,对其中9个化合物进行了结构鉴定,分别为槲皮素-3-O-α-L-鼠李糖苷(Ⅰ)、金丝桃苷(Ⅱ)、槲皮素(Ⅲ)、异莨菪亭(Ⅳ)、α-香树脂醇(Ⅴ)、β-香树脂醇(Ⅵ)、齐墩果酸(Ⅶ)、β-谷甾醇(Ⅷ)、β-胡萝卜苷(Ⅸ)。结论 所有化合物均为首次从该植物中分得。     

瘤果槲寄生化学成分的研究

【目的】研究槲寄生属植物的化学成分。【方法】应用柱层析和结晶方法进行分离、纯化,并采用光谱学方法进行结构鉴定。【结果】分离并鉴定出3个化合物：羽扇豆醇乙酸酯(lupeol acetate)(Ⅰ)、β—香树脂醇(β—amyrin)(Ⅱ)、齐墩果酸(oleanolic acid)(Ⅲ)。【结论】3个化合物均为首次从瘤果槲寄生中分离得到。     

罗布麻茎的化学成分研究

罗布麻系夹竹桃科罗布麻属植物(Apocynum venetum Linn),自茎部分中分离得五个化合物,经理化性质和波谱分析,推定为α-香树脂醇醋酸酯(α-Amyrin acetate,Ⅰ),齐墩果酸醋酸酯(Oleanolio acid acetate,Ⅱ),β-谷甾醇(β-Sitosterol,Ⅲ),β-谷甾醇-β-D-葡萄糖甙(β-Sitosterol-β-D-glucoside,Ⅳ)和羽扇豆醇-3-羟基花生酸酯(Lupeol-3-hydroxyarachidate,Ⅴ),化合物Ⅴ为一新化合物。     

红花化学成分研究(Ⅱ)

目的:研究红花的化学成分.方法:采用溶剂法和柱层析法分离纯化红花中的化学成分,并通过理化性质和光谱数据鉴定所得化合物的分子结构.结果:从红花乙醇提取物中共分得7个单体化合物,经鉴定分别为芹黄素(Ⅰ)、6-羟基山柰酚-3-O-β-D-葡萄糖苷(Ⅱ)、香豆酸(Ⅲ)、对羟基苯甲酰香豆酸酐(Ⅳ)、山柰酚-3-O-β-D-葡萄糖苷(Ⅴ)、cartormin(Ⅵ)和羟基黄色素A(Ⅶ).结论:化合物Ⅳ为红花中首次分离得到.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.