不同发硒水平的大骨节病病区儿童血清酶和元素的分析

对不同发硒水平的大骨节病病区儿童血清酶和微量元素的分析结果显示：３组病区儿童血清酶谱表现一致，其中ＡＬＰ，ＧＯＴ和ＬＤＨ明显高于低硒非病区儿童，提示病区儿童特有的血清酶谱似乎并非由低硒。而各组Ｘ线检出率与发硒水平不呈负相关关系，也提示除低硒外的其它因素与本病发生和发展有关。本实验中发硒水平的差异与病区儿童血清锰、铜、铁、铬、镉、锌等６种元素含量无明显关系。     

急性腹泻病患儿的免疫状态与微量元素改变(附100例病例分析)

对100例急性腹泻患儿做了空腹Ig、淋巴母细胞转化率及微量元素测定,结果表明,急性腹泻患儿低血锌者占47％,低血铁者占34％,低血铜者占40％,IgG降低者占61％,IgA降低者占19％;淋巴母细胞转化率降低者占30％。IgG下降与低锌呈明显正相关。提示急性腹泻患儿存在低锌、低铜、低铁、细胞免疫及体液免疫明显受损。     

老年人冠心病红细胞免疫功能及其与硒的关系

目的;探讨老年人冠心病红细胞免疫功能及机体硒状态。方法：测定２０例老年人冠心病及２４名健康对照者的红细胞免疫粘附功能,同时测定其血浆及红细胞硒含量,血清循环免疫复合物水平。结果：冠心病患者红细胞Ｃ３ｂ受体花环率明显下降,而免疫复合物花环率明显升高;其血浆及红细胞硒含量均较低,血清循环免疫复合物含量升高;红细胞免疫粘附功能降低与血硒水平呈一定的平行关系。结论：老年人冠心病红细胞免疫功能降低,微量元素     

缺铁性贫血,隐性缺铁儿童血清IgG亚类及外周血T细胞亚群的改变

测定47例缺铁儿童(IDA14例,隐性缺铁33例)血清IgG亚类、P_nP_g特异性IgG亚类抗体以及外周血T细胞亚群等,发现其中28例伴IgG亚类缺陷,血清P_nP_g特异性IgG亚类抗体水平、外周血CD_4~+细胞百分率及CD_4~+/CD_g~+比值亦较正常儿童明显下降.提示儿童缺铁不仅损害了T细胞功能,也使B细胞功能明显障碍.     

手足口病患儿早期免疫功能改变及其意义

目的研究手足口病患儿外周血T淋巴细胞亚群、免疫球蛋白及补体变化,探讨其免疫功能改变,为早期免疫干预治疗提供参考依据。方法以临床确诊的50例手足口病患儿为研究对象,应用流式细胞仪检测CD3+、CD4+、CD8+,计算CD4/CD8比值;采用免疫比浊法测定患儿血清IgA、IgG、IgM,C3及C4,并与50名健康正常儿童进行比较。结果患儿血清IgA、IgG、IgM显著低于健康儿童(P0.05),C3及C4水平无明显变化(P0.05);CD3+T细胞水平无差异(P0.05)、CD4+T细胞水平明显下降(P0.01),CD8+T细胞水平明显升高(P0.05),CD4/CD8比值降低(P0.01)。结论手足口病患儿早期存在严重的细胞免疫和体液免疫功能抑制,提示早期进行免疫干预治疗的紧迫性和必要性。     

免疫治疗儿童哮喘螨特异性抗体动态观察

应用ELISA间接法测定了57名哮喘儿童血清螨特异性IgE、IgG、IgA和IgM水平,并对其中15名儿童进行随访观察。结果发现:螨过敏性哮喘儿童血清螨特异性IgE、IgG和IgA水平均高于正常儿童。经螨浸液免疫治疗后血清螨特异性IgE下降、IgG升高。结果表明哮喘儿童存在着多克隆的抗体反应增强。     

肠外营养对胃癌患者化疗期间免疫功能的影响研究

目的:研究肠外营养对胃癌患者化疗期间免疫功能的影响。方法:选取2009年6月~2011年6月我院的100例胃癌患者为研究对象,随机分为2组,肠外营养组50例给予全肠外营养治疗,肠内营养组50例化疗期间给予肠内营养,化疗开始后第1天开始行肠外或肠内营养支持,持续8天。检测并记录化疗前和化疗后第8天患者的细胞免疫指标CD3、CD4、CD8和NKC活性及体液免疫指标IgA、IgG、IgM。结果:肠外营养组化疗后CD3、CD4、CD8值及NKC活性明显下降,IgA、IgG、IgM值明显升高;肠内营养组化疗后CD3、CD4、CD8水平和NKC活性与化疗前相比无显著性变化,IgA、IgG、IgM水平也明显升高。结论:肠外营养对胃癌病人化疗后细胞免疫功能无明显改善作用,肠内营养有明显的细胞免疫增强作用,可有效地纠正胃癌病人化疗后细胞免疫抑制状态。     

硒缺乏对儿童生长发育的影响

目的:儿童中硒缺乏的情况和缺硒对儿童生长发育的影响。方法:收集了680名儿童的基本资料,检测了他们体内血硒的含量,并根据血硒正常与否分组并观察儿童的生长发育状况和免疫力情况,采用SPSS18.0软件进行统计学分析。结果:硒缺乏在儿童中非常普遍,在我们调查中硒缺乏率达到88.67%,并且缺硒普遍存在于各个年龄段、而且女儿童硒缺乏的情况较男儿童严重。硒缺乏的儿童组中身高、体重和智力低于正常水平的例数明显多于血硒水平正常的儿童,出现上呼吸道症状和下消化道症状的例数都要多于血硒水平正常的儿童。结论:硒是一类非常重要的并且不可缺少的微量元素,它对于儿童骨骼肌肉系统和神经系统的发育以及免疫功能的维持具有重要的意义。     

学龄前儿童血红蛋白测定分析

贫血是儿童的常见病之一.贫血对儿童健康的影响更为明显,如造成体力下降,尤其缺乏持久性耐力,细胞免疫水平下降,白细胞吞噬能力减退,容易诱发各种疾病,尤其是感冒、气管炎等上呼吸道感.因此,儿童贫血的防治应引起足够重视.     

硒,维生素E缺乏对大鼠胰岛体密度,数密度,平均体积的影响

体视学定量分析的结果表明，饲予低硒饲料引起大鼠胰岛体密度、平均体积下降、血清胰岛素、Ｃ态水平降低，提示低硒可引起胰岛β细胞结构与功能损害。在饲料中补一定剂量硒和维生素Ｅ明显改善胰岛有关形态学参数及功能状态，表明硒和ＶＥ对胰岛细胞具有良好的保护作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.