多药耐药蛋白在肝癌中的表达及临床意义

目的 探讨肝癌中多药耐药相关蛋白1(MRP1)、肺耐药蛋白(LRP)、P-糖蛋白(Pgp)、胎盘型谷胱甘肽-s-转移酶π(GST-π)和拓扑异构酶Ⅱ(TOPOⅡ)的表达情况,为肝癌的化疗提供依据.方法 选取肝癌手术切除标本26例,取肝癌组织和癌旁肝组织进行MRP1、LRP、Pgp、GST-π和TOPOⅡ检测,采用图像分析系统对结果进行分析.结果 五种多药耐药蛋白在肝癌组织中表达量比癌旁肝组织中高,差异有统计学意义(P＜0.05).Pgp、TOPOⅡ和GST-π在肝癌组织中表达量与肝外转移差异有统计学意义(P＜0.05).五种多药耐药蛋白的阳性率在分化不良肝癌组织高于分化良好肝癌组织,但只有TOPOⅡ差异有统计学意义(P＜0.05).五种多药耐药相关蛋白在肝癌组织中表达量与肿瘤直径、AFP水平、门静脉癌栓、肝硬化情况和肝功能分级之间差异无统计学意义(P＞0.05).结论 五种多药耐药蛋白在肝癌组织中均有不同程度的高表达,且与肿瘤某些生物学行为有关,联合检测有助于选择合理的化疗药物及判断预后.

Abstract：

Objective To investigate the expression of multidrug resistance protein such as multidrug resistance-associated protein 1 (MRP1),lung-resistance related protein (LRP), P-glycoprotein (Pgp),glutathione s-transferase (GST-π) and topoismerase Ⅱ (TOPO Ⅱ ) in hepatocellular carcinoma (HCC), which would be supplied for the clinical chemotherapy of HCC. Methods Twenty-six cases of HCC who underwent hepatectomy were enrolled and immunohistochemical (IHC) staining was carried out on all specimens for the detection of expression of MRP1,LRP,Pgp,GST-πand TOPO Ⅱ and the data was analyzed by image analysis system. Results The expression of five multidrug resistance protein in HCC tissue were significantly higher than those in adjacent tissue beyond cancer (P ＜0.05). The significant differences were found in the expression of Pgp,TOPO Ⅱ and GST-π between HCC tissue and distant metastasis (P ＜ 0.05 ). The expression of the five multidrug resistance protein in poorly differentiated HCC tissue was higher than that in well-differentiated tissue,while the significant difference was only found in the expression of TOPO Ⅱ (P ＜ 0.05 ). The significant association was not found between the expressions of five multidrug resistance protein in HCC tissue and the size of tumor,AFP, the portal vein tumor thrombus,hepatic cirrhosis and liver function. Conclusions Five multidrug resistance protein overexpression in various degrees in HCC tissue, which relates to some biological behavior of the cancer. Combined detection is of much benefit to the choice of the drug of chemotherapy and to the prediction of prognosis.     

Fascin在肺癌中的表达及临床探讨

Objective To investigate Fascin expression in human lung cancer tissues and its clinical significance.Methods Immunohistochemistry was performed in 62 patients with different histological types and clinical stages of lung cancer and 92 cases with other malignant tumors.Fascin positive rate in each group was calculated and the differences of pathological characters between the two groups were analyzed.Results Fascin expression in lung cancer tissues was significantly higher than that in normal lung tissues(P ＜ 0.05),its expression varied by different clinical stage of lung cancer tissue differentiation.As differentiation degree decreased,Fascin positive rate increased.Fascin expression was independent with age,sex,smoking history(P ＞ 0.05).Fascin expression had no significant difference between lung cancer group and other tumor group(P ＞ 0.05).Conclusions Fascin expression raising might be common in malignant tumors.Fascin expression in lung tissues indicated the possibility of lung cancer.In lung tissue,high expression of Fasein was a sign of poor differentiation and malignant status of lung cancer.     

p53和热休克蛋白70在胃肠道间质瘤中的表达及意义

目的 探讨p53和热体克蛋白70(HSP70)在胃肠道间质瘤(GIST)中的表达、与GIST临床病理学特征的关系及两者表达的相关性.方法 应用免疫组织化学SP法检测48例GIST组织中p53和HSP70的表达情况,分析其与临床病理特征的关系,并比较两指标在GIST中表达的相关性.结果 48例GIST中,p53和HSP70阳性表达率分别为60.42%(29/48)和64.58%(31/48).p53和HSP70的表达与GIST的部位、患者性别、年龄无关(P>0.05),与组织学分级有关(P<0.05),p53与HSP70的表达呈正相关(X2=4.075,P<0.05).结论 p53和HSP70在GIST中均有表达,两者均随着GIST侵袭程度的增高其表达明显增加.提示p53和HSP70在GIST的发生、发展中发挥作用,两者可作为判断肿瘤侵袭危险性的客观指标.

Abstract：

Objective To investigate the relationship between the expression of p53,heat shock protein 70(HSP70)and clinieopathologie eharacteristies in gastrointestinal stromal tumor(GIST),and the correlation between p53 and HSP70 expression.Methods p53 and HSP70 expression of 48 cases with GIST was detected by immunohistechemieal SP method.Analyzed the correlation between p53 and HSP70 expression.Results Among 48 cases with GIST,the positive rate for p53 and HSP70 were 60.42%(29/48) and 64.58%(31/48)There was no significant difference in the gender,age of patients.localization of tumor (P>0.05).but there was significant difference in the histological grade (P<005).There was positive correlation between the expression of p53 and HSP70(X2=4.075,P<0.05).Conclusions The positive rate of p53 and HSP70 in GIST is high,especially in hish risk patients.The expressions of p53 and HSP70 play an important role in the pathogenesis and development of GIST,which may be used as an objective parameter for distinguishing malignant GIST from benign cases.     

骨桥蛋白和基质金属蛋白酶-9在胰腺癌中的表达及其临床意义

Objective To investigate the expression and the role of osteopontin(OPN)and matrix metalloproteinase-9(MMP-9)in the invasion and metastasis of pancreatic carcinoma.Methods Human pancreatic cancer cell line MIA PaCa-2 was cultured in vitro.The location of OPN protein in pancreatic tumor cell line was detected by immunofluorescence staining.100 cases of pancreatic cancer and 40 cases of adjacent normal pancreatic tissues were used to analysis.The expressions of OPN and MMP-9 were investigated by immunohistochemistry assay.Results OPN expressed in the cell membrane and cytoplasm,OPN and MMP-9 in pancreatic cancer tissues positive rates were 73.0%(73/100)and 68.0%(68/100),which were higher than that in adjacent normal pancreatic tissues(all P ＜ 0.05).OPN and MMP-9 expression were associated with pancreatic tumor grade,lymph node metastasis and perineural invasion of pancreatic cancer(P ＜ 0.05).OPN and MMP-9 in pancreatic cancer tissues were positively correlated(r,=0.39,P ＜ 0.01).Conclusions OPN and M MP-2 might play an important role in the development of invasion and metastasis of pancreatic carcinoma.OPN and MMP-2 might play synergetic roles in the progression of pancreatic carcinoma.     

The Experiment Study of the Apoptosis,PCNA and the Expression of P53 Protein in the Cervix Carcinoma Tissues

ObjectiveThe purpose of this testWas to study the r elationship between the apoptosis and the proliferation cell nuclear antigen(PCN A).MethodsThe apoptosisWas detected by tagging unselected N-end- labeled and the apoptosisWas detected by immunohistochemistry.ResultsThe positive rate of apoptosis P⁵³ protein and PCNAWere 62.50%,53.13 % and 65.63% respectively in the tissues of 32 cases of cervix squamous cancer. The area of cells’distribution of expression of P⁵³ protein and of expre ssion of PCNA,and thereWas no significantly relationWith apoptosis.ThereWere a few cells of apoptosis and of expressing PCNA only in the epithelial tissue of normal cervix tissue and the number of positive cellsWas less than 10% per mic rosopic field of vision.ConclusionThe P⁵³protein may be parti cipated in the occurring and developing of cervix cancer by advance of cells’ p roliferation and by check of apoptosis.     

人肺腺癌肿瘤细胞免疫表型CD133、CD34、CD44的实验研究

目的 拟验证CD133、CD34、CD44作为人肺腺癌肿瘤干细胞表面标记物的合理性.方法 采集新鲜肺腺癌组织标本,利用两种体外培养方法扩增出贴壁细胞和悬浮细胞球两种肿瘤细胞,采用免疫荧光检测比较CD133、CD34和CD44在两种培养细胞中表达的差异.结果 悬浮球肿瘤细胞培养较贴壁肿瘤细胞生长速度慢、维持时间长且成功率高(72.5% vs 47.5%,P＜0.05).CD133、CD34和CD44在悬浮细胞球中表达率和表达量明显高于贴壁肿瘤细胞(68.97%,82.76%,93.10% vs 5.26%,15.79%,5.26%,P＜0.01).结论 CD133、CD34和CD44可能作为分离人肺腺癌肿瘤干细胞的表面蛋白表标记组合.

Abstract：

Objective To validate the possibility of CD133 CD34 CD44 be served as biomarkers in cancer stem cell of human lung adenocarcinoma. Methods Two kinds of culturing methods were performed to generate adhesive tumor cells and floating aggregates, and the differences of expression of CD133 CD34 CD44 between 2 kinds of cultured cells were observed by immunofluorescence. Results Floating aggregates grew more slowly, kept activity for longer period than adhesive cells (72.5% vs 47.5%,P＜0.05). Floating aggregates expressed higher level of CD133, CD34 and CD44 than adhesive cells (68.97%,82.76%,93.10% vs 5.26%,15.79%,5.26%,P＜0.01). Conclusions The combination of CD133, CD34 and CD44 probably can be used as surface markers of cancer stem cells for human lung adenocarcinoma.     

环氧合酶-2在乳腺癌组织中的表达及其临床病理意义

目的 研究环氧合酶-2(COX-2)在乳腺癌组织中的表达及其与临床病理因素之间的关系.方法 应用免疫组织化学方法检测60例乳腺癌组织中和20例乳腺正常组织中COX-2的表达情况,并结合临床及病理学资料进行分析.结果 COX-2在乳腺癌组织中阳性表达率为65.0%(39/60),在乳腺正常组织中阳性表达率为10.0%(2/20),两者比较差异有统计学意义(P<0.01);且COX-2高表达状态与乳腺癌TNM分期、淋巴结转移和肿瘤细胞表皮生长因子受体2(C-erbB-2)表达明显相关(P<0.05或<0.01).结论 COX-2在乳腺癌组织中表达显著增高,其表达在乳腺癌的发生、发展中具有重要意义.

Abstract：

Objective To investigate the expression of cyclooxygenase-2(COX-2)in breast cancer tissues,as well as the relationship between COX-2 and the clinicopathological features.Method The expression of COX-2 was detected in 60 cases of breast cancer tissues and 20 cases of breast normal tissues by using immunohistochemistry,and combined with clinicopathological information for analysis.Results The COX-2 expression rate was 65.0%(39/60)in breast cancer tissues and 10.0%(2/20)in breast normal tissues respectively.There was statistic difference between the two(P<0.01).The over expression of COX-2 was significantly correlated with TNM stages,lymphatic metastasis and the expression of epidermal growuth factor receptor-2(C-erbB-2)(P<0.05 or<0.01).Conclusion The expression of COX-2 in breast cancer tissues is significantly higher,which might play a fairly important role in tumorigenesis and progression of breast cancer.     

铀矿尘致大鼠肺纤维化过程中Ⅰ和Ⅲ型胶原及层粘连蛋白表达的变化

目的 探讨铀矿尘致大鼠纤维化过程中Ⅰ、Ⅲ型胶原、层粘连蛋白(Laminin,LN)表达的变化特征.方法 60只Wistar大鼠随机分为铀矿尘组(30只)和对照组(30只),铀矿尘组大鼠一次性非暴露气管内滴注20 mg/ml的粉尘悬液1 ml,对照组大鼠一次性气管内滴注生理盐水1 ml,分别于处理后7、14、21、30及60 d每组随机处死6只,取肺组织,HE染色观察肺组织形态学改变,天狼猩红染色法观察肺组织Ⅰ、Ⅲ型胶原变化,免疫组织化学法检测LN蛋白的表达.结果 铀矿尘致肺组织纤维化过程中可见Ⅰ、Ⅲ型胶原的大量增生,染尘后早期主要以Ⅰ型胶原增生为主.铀矿尘组21、30、60d时Ⅰ型、Ⅲ型胶原纤维面积百分比明显增高,与对照组比较,差异均有统计学意义(P＜0.05或P＜0.01).LN主要见于肺组织内血管、支气管周围、血窦壁以及肺间隔的基底膜,形态上以增粗的线状或丛状分布为主.铀矿尘组21、30、60 d时肺组织中LN阳性细胞积分吸光度(14.89±2.25、22.98±2.29、30.34±2.19)增加,与对照组(9.06±1.21、8.98±0.86、10.04±2.00)比较,差异有统计学意义(P＜0.05,P＜0.01).结论 铀矿尘诱导大鼠肺组织纤维化早期以Ⅰ型胶原纤维为主,后期Ⅰ型、Ⅲ型胶原纤维均大量增多,分布范围扩大.肺组织过程中有LN高表达.

Abstract：

Objective To explore the characteristics of LN and type Ⅰ, Ⅲ collagen in pulmonary fibrosis induced by uranium ore dust in rats. Methods 60 adult Wistar rats were divided randomly into two groups, control group (30 rats)and uranium ore dust group (30 rats). Non-exposed intratracheal instillation method was used. Uranium ore dust group was exposed 20 mg/ml uranium ore dust suspension 1ml per rat,meanwhile control group was exposed normal saline 1ml per rat. Post-exposed the 7, 14, 21, 30 and 60 d, 6 rats in each group were killed randomly, lung tissue were collected. The pathological changes in lung tissue were observed by microscope using HE staining, the collagen Ⅰ and Ⅲ in lungs were observed by polarizing microscope using Biebrich scarlet staining. The expression of LN protein in lung tissue was observed by immunohistochemistry-SP. Results During lung fibrosis, a large amount of the proliferated Ⅰ and Ⅲ collagen in lungs were observed. Post-exposure to uranium ore dust, the characteristics in proliferated collagen in lungs were type Ⅰ collagen deposited in lung interstitium mainly in the early stage. The area percentage of collagen Ⅰ and Ⅲ was increased significantly at 7,14, 21, 30 and 60 d in the experimental group as compared with that in the control group (P＜0.05 or P＜0.01). The over expression of LN in the lung tissue were observed. The expression of LN was distributed in the lung tissue as thickening of the linear or cluster. The integral optical density of LN was increased significantly at 21,30 and 60d in the experimental group as compared with that in the control group(P＜0.05 or P＜0.01). Conclusions After exposure to uranium ore dust, the characteristics in proliferated collagen in lungs are the type of Ⅰ collagen deposited in lung interstitium mainly in the early stage, while the type of Ⅲ collagen increase significantly at the later period. The overexpression of LN exists in the process of pulmonary fibrosis. It suggests that LN has a role effect in the process of pulmonary fibrosis.     

Ras相关核蛋白在肺鳞癌组织中的表达及意义

目的探讨肺鳞癌组织中Ras相关核蛋白(Ran)的mRNA和蛋白表达水平变化及其意义。方法采用实时荧光定量PCR(RQ-PCR)和Western Blot技术检测22例不同分期及分化程度的肺鳞癌组织及其相应癌旁正常组织中Ran的表达水平,并分析其与临床病理指标的关系。结果肺鳞癌组织中Ran mRNA表达水平是癌旁正常组织的2.35倍(P0.05),Ran在肺鳞癌组织中的蛋白表达水平高于癌旁组织。Ran在非早期(Ⅲ期)肺鳞癌组织中mRNA和蛋白的表达水平均高于早期(Ⅰ期+Ⅱ期)癌组织(P0.05),在低分化肺鳞癌组织和高、中分化癌组织中mRNA和蛋白表达水平的差异无统计学意义(P0.05)。结论 Ran的表达上调可能与肺癌的发生、发展相关。     

G蛋白偶联受体81、单羧酸转运体1、单羧酸转运体4蛋白在宫颈鳞癌及其癌前病变中的表达和临床意义

目的研究GPR81、MCT1、MCT4蛋白在宫颈鳞癌及其癌前病变中的表达和临床意义。方法选择2016年1月-2018年10月于本院经手术获取的156例患者的宫颈病变组织进行观察,其中55例为宫颈鳞癌、73例为宫颈上皮内瘤变(CIN)、28例为正常宫颈组织。选择免疫组化SP法对宫颈鳞癌、CIN组织、正常宫颈组织内GPR81、MCT1、MCT4蛋白阳性表达率进行测定,并分析GPR81、MCT1、MCT4蛋白和宫颈鳞癌相关临床病理参数相关性。结果宫颈鳞癌组、CIN组患者的病变组织内GPR81、MCT1、MCT4蛋白阳性表达率均高于正常宫颈组,宫颈鳞癌组高于CIN组,差异有统计学意义(P<0.05);Ⅲ期~Ⅳ期宫颈鳞癌患者的MCT1、MCT4蛋白阳性表达率与Ⅰ期~Ⅱ期患者相比,差异无统计学意义(P>0.05);有淋巴结转移患者的GPR81、MCT1、MCT4蛋白阳性表达率与无淋巴结转移患者相比,差异无统计学意义(P>0.05);高分化患者的GPR81、MCT1、MCT4蛋白阳性表达率与中低分化患者对比,差异无统计学意义(P>0.05);而GPR81、MCT1、MCT4蛋白于宫颈鳞癌组织内相关表达无显著相关性(P均>0.05)。结论GPR81、MCT1、MCT4蛋白宫颈鳞癌及其癌前病变中表达均增高,故积极检测GPR81、MCT1、MCT4蛋白表达能帮助临床医师诊治宫颈鳞癌及其癌前病变提供信息。     

肿瘤转移抑制蛋白及上皮型钙黏蛋白在上尿路移行上皮癌中的表达

目的探讨肿瘤转移抑制蛋白（MTSS1）与上皮型钙黏蛋白（E-cadherin）在上尿路移行上皮癌中的表达情况及意义。方法收集病理科存档的60例上尿路移行上皮癌石蜡标本为观察组，同时取其中30例患者的膀胱正常组织作为对照组。采用免疫组织化学方法检测组织中MTSS1及E-cadherin的表达情况，并分析其表达与不同病理分期、分化程度及淋巴结转移间的关系。结果MTSS1在对照组的表达率为100．0％（30／30），在观察组表达率为45．0％（27／60），两组比较差异有统计学意义（P〈0．05）；E-cadherin在对照组表达率为96．7％（29／30），在观察组表达率为41．7％（25／60），两组比较差异有统计学意义（P〈0．05）；MTSS1与E—cadherin在不同病理分期、分化程度及淋巴结有无转移中表达率差异有统计学意义，分化程度好、TNM分期低、淋巴结无转移的患者中MTSS1与E-cadherin的表达率较高（P〈0．05）；MTSS1与E—cadherin在癌组织中的表达并无相关性（P〉0．05）。结论在上尿路移行上皮癌中联合检测MTSS1及E—cadherin的表达，对于判断肿瘤的恶性程度、淋巴结转移情况及患者的预后均有重要意义。     

p57~(kip2)和PCNA蛋白在西宁地区食管鳞癌中的表达

目的分析p57kip2和PCNA蛋白在西宁地区食管鳞癌组织中的表达,探讨其在食管鳞癌发生发展中的意义。方法选取60例食管鳞癌及其相对应的31例癌旁正常组织标本,应用免疫组化方法检测p57kip2和PCNA蛋白,并结合临床病理特点分析。结果p57kip2在食管鳞癌中的表达率为28.33%,低于癌旁正常组织的表达率(51.61%),并与食管鳞癌的分化程度、浸润深度有关,差异有统计学意义(P<0.05)。PCNA在食管鳞癌中的表达率为81.67%,高于癌旁正常组织的表达率(61.29%),与食管鳞癌的分化程度、浸润深度、淋巴结转移有关,差异有统计学意义(P<0.05)。p57KIP2与PCNA蛋白的表达呈负相关(rs=-0.611,P<0.01)。结论食管鳞癌组织中p57kip2低表达与PCNA的高表达共同参与食管癌的发生、发展。联合检测可作为预测食管鳞癌恶性程度和预后的重要指标。     

宫颈鳞癌中EZH2和Ki67的表达及其临床病理意义

目的:探讨EZH2和Ki67在宫颈鳞癌中的表达及其临床意义。方法:应用免疫组织化学S-P法,检测40例宫颈鳞癌组织、10例CIN组织、10例正常宫颈组织中EZH2和Ki67的表达及其与临床病理特征的相关性,并探讨二者的相关性。结果:EZH2在正常宫颈组织、CIN、宫颈鳞癌组织中的阳性表达率分别为30.0%,70.0%,92.5%。Ki67在正常宫颈组织、CIN、宫颈鳞癌组织中的阳性表达率分别为10.0%,30.0%,95.0%。EZH2蛋白表达阳性率在淋巴结转移组明显高于无淋巴结转移组,差异有统计学意义(P<0.05)。Ki67蛋白表达与临床特征均无关(P>0.05)。EZH2与Ki67表达间差异无统计学意义(P>0.05)。结论:在正常宫颈组织、CIN和宫颈鳞癌组织中,EZH2和Ki67蛋白表达的阳性率均呈逐渐升高趋势,表明均参与了宫颈鳞癌的发生发展;二指标的无相关性,表明EZH2间接调节了癌细胞的增殖。     

宫颈鳞癌中β-catenin和DKK-1的表达及相关性研究

目的:研究β-catenin蛋白及DKK-1(Dickkopf-1)蛋白在宫颈鳞癌中的表达,探讨两者在宫颈鳞癌侵袭转移中的作用和机制,分析DKK-1表达水平改变时对宫颈鳞癌的侵袭能力和Wnt信号通路活性的影响。方法:应用免疫组织化学染色方法检测54例宫颈鳞癌组织、20例正常宫颈组织中β-catenin的异常表达和DKK-1蛋白的阳性表达,并分析不同宫颈组织临床及病理因素之间的关系。结果:宫颈鳞癌中DKK1蛋白的阳性表达低于正常宫颈组织,而β-catenin蛋白的异常表达高于正常宫颈组织(P<0.01);DKK-1和β-catenin蛋白在宫颈鳞癌中的表达与肿瘤分化程度、临床分期及有无淋巴转移有关(P<0.05)。宫颈鳞癌中DKK1的阳性表达与β-catenin的异常表达呈负相关(r=-0.465,P<0.01)。结论:DKK-1表达的减少或缺失与宫颈鳞癌的发生和发展密切相关,其机制可能与活化Wnt/β-catenin信号通路有关。     

CD_(44)v6、MMP-2在子宫内膜癌的表达及临床意义

目的探讨子宫内膜癌组织中基质金属蛋白酶-2(matrix metalloproteinase,MMP-2)及粘附分子CD44拼构变异体6(CD44splice variant 6,CD44v6)的表达,分析其与子宫内膜癌浸润、转移的关系。方法选取73例子宫内膜癌组织标本及同期正常增生期子宫内膜27例为对照组。用免疫组织化学方法检测CD44v6、MMP-2的表达。结果 CD44v6在EC中的阳性表达显著高于正常增生期子宫内膜(P<0.05);CD44v6与肌层浸润深度有关(P<0.05);CD44v6的阳性表达率随淋巴结转移和病理分级增高有下降的趋势,但差异无统计学意义(P>0.05)。MMP-2在子宫内膜癌中的阳性表达显著高于正常增生期子宫内膜(P<0.05);MMP-2仅与肌层有无浸润有关(P<0.05)。CD44v6和MMP-2在EC组织中的表达呈正相关性(rs=0.268,P<0.05)。结论 EC中CD44v6及MMP-2高表达的患者更易发生浸润、转移,且二者有正协同作用。联合检测CD44v6、MMP-2可作为判断子宫内膜癌恶性程度,预测转移和评估预后的生物学指标。     

DJ-1在子宫内膜癌中的表达及临床意义

目的:探讨DJ-1基因mRNA及其蛋白在子宫内膜癌、癌旁组织及正常子宫内膜组织中的表达规律及临床意义。方法:应用逆转录聚合酶链反应(RT-PCR)、蛋白印迹(Western blot)及免疫组织化学Elivision法检测100例子宫内膜癌、癌旁组织及30例正常子宫内膜组织中DJ-1基因mRNA及其蛋白的表达情况,分析DJ-1基因在子宫内膜癌组织中的表达与肿瘤的病理类型、分化程度、浸润深度、淋巴结转移、期别及患者血CA125水平等临床病理特征之间的关系。结果:子宫内膜癌组织中的DJ-1 mRNA及DJ-1蛋白的表达均明显高于癌旁组织及正常子宫内膜组织,癌旁组织及正常子宫内膜组织间表达无显著差异。DJ-1 mRNA的表达与子宫内膜癌的病理类型、期别及血CA125水平无明显相关性;中低分化的子宫内膜癌组织中DJ-1 mRNA的表达高于高分化者,深肌层浸润的子宫内膜癌组织中DJ-1 mRNA的表达高于局限于黏膜层及浅肌层浸润者,DJ-1 mRNA在有淋巴结转移的患者中的表达高于无淋巴结转移者。结论:DJ-1 mRNA及蛋白在子宫内膜癌组织中高表达,且可能参与了子宫内膜癌的发展、浸润和转移等环节的调控。     

VEGF-C、nm23 H1在宫颈增生性病变中的表达及临床意义

目的：探讨血管内皮生长因子C（ VEGF-C）和肿瘤转移抑制基因（ nm23 H1）在宫颈增生性病变发生、发展过程中的表达及其临床意义。方法采用免疫组织化学法检测51例宫颈鳞状细胞癌组织中VEGF-C和nm23 H1的表达水平,以31例宫颈炎性、修复性增生组织,27例宫颈上皮内瘤变组织做对照,对结果进行分析。结果在宫颈炎性、修复性增生组织,宫颈上皮内瘤变及宫颈鳞状细胞癌组织中,VEGF-C的表达分别为3．2％、40．7％、72．5％,3组间比较有显著性差异（χ^2＝37．702,P＜0．01）;nm23 H1的表达分别为100．0％、59．2％、33．3％,3组间比较有显著性差异（χ^2＝35．349,P＜0．01）。在宫颈鳞癌组织中, VEGF-C和nm23 H1在伴有淋巴结转移的组织中阳性表达率为87．5％和16．7％;未伴淋巴结转移中阳性表达率为59．3％和48．1％。在宫颈增生性病变组织中,无论有无淋巴结转移,VEGF-C与nm23 H1阳性表达始终呈负相关（r＝-0．532,P＜0．05）。结论 VEGF-C和nm23 H1存在相关性,并在宫颈鳞癌的发生、发展、预后和转移方面发挥着重要作用,其可作为评价宫颈病变、推测预后的重要参考指标。     

二磷酸核苷激酶在云锡矿工肺癌中的表达及其与淋巴结转移的关系

目的研究ｎｍ２３基因的表达产物二磷酸核苷激酶（ＮＤＰＫ）在云锡矿工肺癌中的表达及其与肺癌淋巴结转移的关系。方法免疫组化ＬＳＡＢ法。结果ＮＤＰＫ在云锡矿工肺癌中有较高表达,阳性率５７．９％（３３／５７）,其中,鳞癌５６．４％（２２／３９）、腺癌６１．１％（１１／１８）,差异无显著意义（Ｐ＞０．０５）。在云锡矿工肺鳞癌中,无肺门或纵隔淋巴结转移者阳性率为６２．０％（１８／２９）,强阳性占阳性的６６．６％（１２／１８）;有肺门或纵隔淋巴结转移者阳性率为４０．０％（４／１０）,强阳性为０（０／４）。二者差异有显著意义（Ｐ＜０．０５）。ＮＤＰＫ的表达与云锡矿工肺腺癌有、无肺门或纵隔淋巴结转移之间,差异无显著意义（Ｐ＞０．０５）。结论ｎｍ２３基因可能在云锡矿工肺鳞癌和肺腺癌形成及转移过程中起不同的调节作用,其表达程度与云锡矿工肺鳞癌淋巴结转移呈负相关;未发现其与云锡矿工肺腺癌淋巴结转移有相关关系。