REGULATING EFFECTS OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND ANG Ⅱ ON FROG''''S PERICARDIAL STOMATA, MESOTHELIUM AND ANGIOGENESIS

INTRODUCTION Vascular endothelial growth factor (VEGF) is a dimerous polypeptide with a molecular weight of 46kD. Its target is the endothelial cells, especially promoting their mitochysis (1) . The application of VEGF protein or trans- VEGF gene technique could enhance collateral circulation in the ischemic myocardium and extremities and EDRF- dependent expansion of the coronary vessels, as well as improve the blood supply of the ischemic areas (2).The new study revealed that VEG…     

REGULATING EFFECTS OF VASCULAR ENDOTHELIALGROWTH FACTOR AND ANG 11 ON FROG‘S PERICARDIAL STOMATA, MESOTHELIUM AND ANGIOGENESIS

OBJECTIVE: To observe the regulating effects of vascular endothelial growth factor (VEGF) and angiotensin II (ANG II) on the frog's pericardium, lymphatic stomata and angiogenesis so as to reveal their effects and mechanism on the mesothelial permeability, lymphatic stoma regulation and myocardial hypertrophy. METHODS: VEGF and ANG II were injected into the frog's peritoneal cavity so as to examine the changes of the pericardial stromata by using transmission electron microscopy, scanning electron microscopy and computerized imaging analysis. RESULTS: Scattered distributed pericardial stomata were found on the parietal pericardium of the frog with a few sinusoid mesothelial cells, whose blood supply was directly from the cardiac chambers flowing into the trabecular spaces of the myocardium (because there are no blood vessels in the myocardium of the frog). The average diameters of the pericardial stomata in VEGF and ANG II groups were 1. 50 microm and 1.79 microm respectively, which were much larger than those in the control group (0.72 microm, P < 0.01); the average distribution densities of the stomata were 8. 25/0.1 mm2 and 12.80/0.1 mm2 in VEGF and ANG II groups, which were also much higher than those in the control group (3.57/0.1 mm2, P < 0.01); the sinusoid areas in VEGF and ANG II groups were 2442.95 microm2/0.1 mm2 and 2121.79 microm2/0.1 mm2, which were larger than that in the control group (995.08 microm2/0.1 mm2 , P < 0.01); no angiogenesis was found in the frogs of the experimental groups. CONCLUSIONS: VEGF and ANG II could strongly regulate the pericardial stomata by increasing their numbers and openings with larger diameters and higher distribution density. They could also increase the sinusoid areas with the result of the higher permeability of the pericardium, which clearly indicated that VEGF and ANG II could speed up the material transfer of the pericardial cavity and play an important role in preventing myocardial interstitial edema. Yet there was no strong evidence to show the angiogenesis in the myocardium.     

THE EFFECT OF BASIC FIBROBLAST GROWTH FACTOR SLOW-RELEASE MICROCAPSULES ON ANGIOGENESIS IN INFARCTED RABBIT MYOCARDIUM

ＩＮＴＲＯＤＵＣＴＩＯＮ　　Ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ (ｂＦＧＦ)ｉｓａｎｉｎｔｅｎｓｅｌｙｍｉｔｏｇｅｎｉｃａｓｗｅｌｌａｓａｎｇｉｏｇｅｎｉｃｓｉｎｇｌｅｃｈａｉｎ 1 7 2ｋＤｐｅｐ ｔｉｄｅ ,ｗｈｉｃｈｉｓｗｉｄｅｌｙｄｉｓｔｒｉｂｕｔｅｄｉｎｂｌｏｏｄｖｅｓｓｅｌｓ ,ｃａｒｄｉａｃｔｉｓｓｕｅｓａｎｄｏｔｈｅｒｏｒｇａｎｓ .ＩｎｖｉｖｏａｄｍｉｎｉｓｔｒａｔｉｏｎｏｆｂＦＧＦｌｅａｄｓｔｏｍｙｏｃａｒｄｉｕｍａｎｇｉｏｇｅｎｅｓｉｓｔｈａｔｈａｓｂｅｅｎｄｅｍｏｎ…     

EFFECTS OF TRANSFORMING GROWTH FACTOR β AND RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN 2 ON HUMAN PERIODONTAL LIGAMENT FIBROBLASTS

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EFFECTS OF CERTAIN VASOACTIVE PEPTIDES ON PATHOGENESIS OF VASCULAR RESTENOSIS

Objective. To investigate the effects of several vasoactive peptides on the development of arterial restenosis after balloon angioplasty.Methods. In rat aortic artery restenosis model produced by denudation of aortic endothelia, we observed changes of endothelin (ET), angiotensin II (AII), calcitonin gene-related peptide (CGRP) and adrenomedullin (Adm) in plasma and aorta with radioimmunoassay and expression of hypertension-related gene (HRG-1) with semi-quantitative RT-PCR, and studied the effects of these peptides on intimal hyperplasia, intima/media ratio and MAPK activities of aortic artery after angioplasty respectively. Furthermore, in cultured cells, we studied the effects of these peptides on vascular smooth muscle cell (VSMC) proliferation and expression of HRG-1 of VSMC from spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats with 3H-TdR incorporation and RT-PCR respectively.Results. After angioplasty, the levels of ET and AII in plasma and aorta significantly increased, accompan     

INHIBITORY EFFECT OF ANGIOTENSIN Ⅱ TYPE 1 RECEPTOR-ASSOCIATED PROTEIN ON VASCULAR SMOOTH MUSCLE CELL GROWTH AND NEOINTIMAL FORMATION

Objective To investigate the mechanism of a novel angiotensin n type 1 receptor-associated protein (ATRAP) interfering with angiotensin Ⅱ type 1 (AT1) receptor-mediated vascular smooth muscle cell (VSMC) growth and neointimal formation. Methods VSMCs isolated from thoracic aorta of adult Sprague-Dawley ( SD) rats were used in this study. ATRAP Cdna was subcloned into pcDNA3 vector and then transfected into VSMCs. DNA synthesis and extracellular signal-regulated kinase (ERK) and phospho-ERK expressions in VSMCs were assayed by measurement of 3H thymidine incorporation and Western blotting, respectively. Morphological changes were observed in the balloon injured artery with or without transfection of ATRAP Cdna using 12-week-old male SD rats. Results ATRAP overexpression in VSMCs inhibited angiotensin Ⅱ (Ang Ⅱ) -induced 3H thymidine incorporation 48 hours after Ang Ⅱ stimulation (P ＜ 0.05). In VSMC, Ang Ⅱ stimulation increased the phosphorylation of ERK, which reached the peak around 60 minutes. The activation of phospho-ERK was significantly decreased by ATRAP (P ＜ 0.05). Neointimal formation was markedly inhibited by ATRAP overexpression in injuried arteries. Conclusions The AT1 receptor-derived activation of ERK plays an essential role in Ang Ⅱ-induced VSMC growth. The growth inhibitory effects of ATRAP might be due to interfering with AT1 receptor-mediated activation of ERK in VSMC growth and neointimal formation.     

INHIBITORY EFFECT OF ANGIOTENSIN Ⅱ TYPE 1 RECEPTOR-ASSOCIATED PROTEIN ON VASCULAR SMOOTH MUSCLE CELL GROWTH AND NEOINTIMAL FORMATION

Objective To investigate the mechanism of a novel angiotensin Ⅱ type 1 receptor-associated protein （ATRAP） interfering with angiotensin Ⅱ type 1 （AT1） receptor-mediated vascular smooth muscle cell （VSMC） growth and neointimal formation. Methods VSMCs isolated from thoracic aorta of adult Sprague-Dawley （SD） rats were used in this study. ATRAP cDNA was subcloned into pcDNA3 vector and then transfected into VSMCs. DNA synthesis and extracellular signal-regulated kinase （ERK） and phospho-ERK expressions in VSMCs were assayed by measurement of ^3H thymidine incorporation and Western blotting, respectively. Morphological changes were observed in the balloon injured artery with or without transfection of ATRAP cDNA using 12-week-old male SD rats. Results ATRAP overexpression in VSMCs inhibited angiotensin Ⅱ （Ang Ⅱ）-induced ^3H thymidine incorporation 48 hours after Ang Ⅱ stimulation （ P 〈 0. 05 ）. In VSMC, Ang Ⅱ stimulation increased the phosphorylation of ERK, which reached the peak around 60 minutes. The activation of phospho-ERK was significantly decreased by ATRAP （ P 〈 0. 05 ）. Neointimal formation was markedly inhibited by ATRAP overexpression in injuried arteries. Conclusions The AT1 receptor-derived activation of ERK plays an essential role in Ang Ⅱ-induced VSMC growth. The growth inhibitory effects of ATRAP might be due to interfering with AT1 receptor-mediated activation of ERK in VSMC growth and neointimal formation.     

EXPRESSION OF MATRIX METALLOPROTEINASE-7 INVOLVING IN GROWTH, INVASION, METASTASIS AND ANGIOGENESIS OF GASTRIC CANCER

Objective. To investigate the role of matrix metalloproteinase-7 (MMP-7) expression in caricinogenesis and progression of gastric cancer.Methods. We studied MMP-7 expression and microvessel density (MVD) in adjacent mucosa and primary foci of 113 cases of gastric cancer by streptavidin -biotin -immunoperoxidase method using anti -MMP-7 and anti-CD34 antibodies. MMP-7 expression and mean MVD were compared with clinicopatholog-ical features of gastric cancer, with the relationship between MMP-7 expression and MVD concerned in gastric cancer.Results. MMP-7 showed positive expression in adjacent mucosa of gastric cancer (29.20%, 33/113), less than that in gastric cancer (69.03%, 78/113). MMP-7 expression in primary foci of gastric cancer was positively correlated with tumor size, invasive depth, metastasis and TNM staging (P<0.05), but not with differentiation or growth pattern of gastric cancer (P>0.05). Positive correlation of mean MVD with tumor size, invasive depth, metastasis and TNM staging was found     

EFFECTS OF POLLEN TYPHAE, VIT E AND VIT C ON THE SYNTHESIS OF PROSTACYCLIN AND PRODUCTION OF LIPID PEROXIDE BY THE CULTURED RAT ENDOTHELIAL CELLS

It is well known that hyperlipemic serum can cause injury to the vascular endothelial cells. The toxic effect may be due to lipid peroxides. Rat endothelial cells were cultured in hyperlipemic sera with Pollen Typhae components, Vit E (16μg/ml) and Vit C (100μg/ml) added separately. Pollen Typhae and Vit C significantly stimulated the synthesis of prostacylin (determined as 6-oxo-PGP_(1α) by radioimmunoassay) by the cultured cells. Vit E and Vit C markedly decreased the amount of MDA (malondialdehyde, the end product of lipid peroxides, determined by TBA reaction) in these cells. These results indicated that Pollen Typhae, Vit E and Vit C may have some beneficial effect in prevention and treatment of atherosclerosis.     

BOOSTING EFFECTS OF DRUGS BENEFITING VITAL ENERGY AND ACTIVATING BLOOD AND TRANSFER FACTOR ON THE IMMUNITY OF PATIENTS WITH CHRONIC COR PULMONALE

The immunity of patients with chroniccor pulmonale has been investigated and com-pared with that of healthy individuals.Cel-lular immunity was found markedly lowerthan in healthy individuals.In order to im-prove immunity and resistance to disease in     

INHIBITORY EFFECT OF ANGIOTENSIN II TYPE 1 RECEPTOR-ASSOCIATED PROTEIN ON VASCULAR SMOOTH MUSCLE CELL GROWTH AND NEOINTIMAL FORMATION

ANGIOTENSIN II type1(AT1) receptor is akey mediator in the effect of angiotensin I(Ang II) on cardiovascular remodeling aswelas homeostasis.1Several studies have raised the possibility that theC-term inal cytoplasm ic domain of this receptor in- teractswi…     

EFFECTS OF ACUPUNCTURE AND INTRAVENOUS ANESTHESIA ON CARDIOVASCULAR FUNCTION

We have reported less blood pressure changesin open heart surgery with acupuncture as com-pared with intravenous anesthesia.Since 1980we have studied separately the effects of acupunc-ture and intravenous anesthesia using the par-ameters of the impedance cardiogram,cardiaccontractility and pulmonary diastolic pressure indog experiments,and also studied the effects em-ploying the impedance cardiogram in open heartsurgery as an index to evaluate the patients' car-diac performance.     

EFFECTS OF STEROIDS ON CO-CULTURE OF EMBRYOS AND ENDOMEDRIUM IN MICE

An attempt has been madc to dcvelop an in vitro system for the study of implantation in mice. Endomctrial cells were obtained from adult fertile ICR mice on Day I of pregnancy.Cells were cultured for 4 days in one of the following media: I) 0.2 ml CEHFM; 2) 0.2 ml     

EFFECTS OF ANTI-PML/RARα OR ANTI-PML ANTISENCE ON THE GROWTH, DIFFERENTIATION AND APOPTOSISOF NB4 CELL LINES

R6sumeobjectifPourdluciderleseetsd,oligonucleotidesantisensantiusPML/RARaetanti-PMLsurcroissance,dmerenciationetaPoPtosedeligniecellulaireNB4.MdthodesColorationparWrightetaitemPloyeepourobserverlamorPhologiecellulaire,flowucytometrieetelectroPhor6sedeDNAsurgelpouraPoPtosecellulaire,essaiparculturesurmethylcellulosepourmesured'unitedeformationdecoloniedescellulesleucemiques(AML-CFU)etexclusiondecolorationpartryPanbleupournumerationdescellulesvivantes.R/suItatsLesoligonucldotidesant…     

STUDIES ON HAWTHORN AND ITS ACTIVE PRINCIPLE Ⅱ.EFFECTS ON CULTURED RAT HEART CELLS DEPRIVED OF OXYGEN AND GLUCOSE

Vitexin rhamnoside(VR)is one of the activeprinciples extracted from the leaves of hawthorn(Crataegus pinnatifida Bge.vat.major N.E.Br.).Theaction of VR on the performance of cultured ratheart cells and rat heart ceils deprived of oxygen andglucose was observed.Results showed no evidentchanges in the heart beat rate,rhythm,range orstrength of heart ceil clusters before and after me-dication.VR did not counter the actions of ouabainand aconitine at cellular levels.Results also demon-strated VR's protective action on cultured heart cellsdeprived of oxygen and glucose.     

EFFECTS OF GLYCYRRHIZAE AND GLYCYRRHIZICACID ON CELLULAR IMMUNOCOMPETENCE OF γ-RAY-IRRADIATED MICE

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EXPERIMENTAL AND CLINICAL RESEARCH OF THE EFFECTS OF ISOFLURANE ON CIRCULATORY FUNCTION

The purpose of this study was to observe the circulatory effects of Isoflurane on animals and patients, using both invasive and noninvasive methods and to evaluate the possibility of application of this general anesthetic during cardiac operation. According to various MAC. the results were divided into two groups in experimental research (8 dogs ). i.e. group Ⅰ(n=41)<1 MAC, group Ⅱ(n=27)>1 MAC, and in clinical study (31 patients), there were 3 groups, group Ⅰ(n=104)<1 MAC, group Ⅱ(n=59)>1 MAC≤2 MAC, group Ⅲ(n=15)>2 MAC. The main changes following isoflurane were as follows: 1. Preload (CVP, PCWP) CVP elevated both in animals and patients (P>0.05), PCWP increased significantly in Clinical Ⅰand Ⅱ(P<0.05), 2. Afterload (MAP, SVR, PAP, PVR) MAP and SVR decreased in both animals and patients MAP (P<0.01), SVR (P>0.05), but PAP and PVR elevated in both group (P>0.05) except in Clinical ⅢPAP and PVR were increasing (P<0.01, P<0.05); 3. Myocardial contractility, with no variation in all patients (P>0.05), but depressed in animals (P<0.05); 4. HR increased (P<0.01) but no arrhythmia; 5. SV showed no change in animals (P>0.05) but decreased in patients (P<0.05), and CO had no change in both animals and patients. It demonstrated that the effects of Isoflurane on cardiovascular function in animals or patients with noncardiac diseases were quite minimal. Although the deteriorative effect on myocardial function in patients with heart disease was less than that of Enflurane and Fluothane, during administering Isoflurane in open heart surgery especially in poor cardiac function, Isoflurane combined with intravenous anesthesia was suggested.     

EXPERIMENTAL STUDY ON ISCHEMIA AND REPERFUSION INJURY OF RAT LIVER AND EFFECTS OF LIGUSTRAZINE AND SALVIA COMPOUND

ＥＸＰＥＲＩＭＥＮＴＡＬＳＴＵＤＹＯＮＩＳＣＨＥＭＩＡＡＮＤＲＥＰＥＲＦＵＳＩＯＮＩＮＪＵＲＹＯＦＲＡＴＬＩＶＥＲＡＮＤＥＦＦＥＣＴＳＯＦＬＩＧＵＳＴＲＡＺＩＮＥＡＮＤＳＡＬＶＩＡＣＯＭＰＯＵＮＤＷｕＷｅｉｇｕｏ（武卫国）ａｎｄＱｉｕＦａｚｕ（裘法祖...